Dickeya phage vB_DsoM_JA13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Salmondvirus; unclassified Salmondvirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 323 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A384ZWQ1|A0A384ZWQ1_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA13 OX=2283030 GN=JA13_275 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.76SMTRR6 pKa = 11.84VISILAGTDD15 pKa = 3.36EE16 pKa = 4.65LSEE19 pKa = 4.09AFISVLHH26 pKa = 6.36KK27 pKa = 10.91ADD29 pKa = 2.97VDD31 pKa = 4.28VNVDD35 pKa = 2.84IDD37 pKa = 4.96RR38 pKa = 11.84VILLNDD44 pKa = 3.49YY45 pKa = 11.04GSATLVPNDD54 pKa = 3.38NAVEE58 pKa = 4.1IEE60 pKa = 4.01SSNLHH65 pKa = 5.88GSRR68 pKa = 11.84VIINDD73 pKa = 3.63PVSALLFALYY83 pKa = 10.32RR84 pKa = 11.84LNLRR88 pKa = 11.84LDD90 pKa = 3.76VEE92 pKa = 5.19DD93 pKa = 4.21EE94 pKa = 4.21DD95 pKa = 4.95QIGVLPTGYY104 pKa = 9.38PVLPEE109 pKa = 4.07PRR111 pKa = 11.84CTFADD116 pKa = 4.01HH117 pKa = 6.5SAKK120 pKa = 10.52AYY122 pKa = 9.36TKK124 pKa = 10.44EE125 pKa = 3.95QMLEE129 pKa = 3.93YY130 pKa = 10.79ARR132 pKa = 11.84DD133 pKa = 3.35AVVRR137 pKa = 11.84FTGMAPFEE145 pKa = 4.41IEE147 pKa = 3.98ADD149 pKa = 3.66PVPFDD154 pKa = 5.2DD155 pKa = 4.57MYY157 pKa = 11.44KK158 pKa = 8.66EE159 pKa = 5.02HH160 pKa = 7.02AVEE163 pKa = 3.97WCDD166 pKa = 3.41TCGGQIGDD174 pKa = 4.22DD175 pKa = 3.85CSCEE179 pKa = 4.32DD180 pKa = 3.77EE181 pKa = 5.37EE182 pKa = 6.25GDD184 pKa = 5.09EE185 pKa = 4.49DD186 pKa = 5.51EE187 pKa = 4.52EE188 pKa = 5.19WEE190 pKa = 4.96DD191 pKa = 5.15ADD193 pKa = 5.83CEE195 pKa = 4.53HH196 pKa = 7.14CGCTNCDD203 pKa = 3.28CDD205 pKa = 3.79IDD207 pKa = 4.22EE208 pKa = 6.31DD209 pKa = 4.74SICSKK214 pKa = 10.41CHH216 pKa = 6.14SASCQCLPDD225 pKa = 4.06FDD227 pKa = 4.63GQEE230 pKa = 4.13DD231 pKa = 4.25PEE233 pKa = 4.39QVKK236 pKa = 9.89EE237 pKa = 4.03EE238 pKa = 4.13RR239 pKa = 3.87

Molecular weight: 26.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A384ZWQ0|A0A384ZWQ0_9CAUD Uncharacterized protein OS=Dickeya phage vB_DsoM_JA13 OX=2283030 GN=JA13_254 PE=4 SV=1
MM1 pKa = 7.23RR2 pKa = 11.84TSLTVAIMALVAKK15 pKa = 8.05QHH17 pKa = 6.12NIEE20 pKa = 4.46VVCSQSIEE28 pKa = 3.97NDD30 pKa = 3.33ATCGWFEE37 pKa = 4.12RR38 pKa = 11.84EE39 pKa = 3.82RR40 pKa = 11.84EE41 pKa = 4.11QRR43 pKa = 11.84SAPHH47 pKa = 5.38WQTRR51 pKa = 11.84AQYY54 pKa = 10.47KK55 pKa = 10.47KK56 pKa = 10.43LVLPKK61 pKa = 10.08RR62 pKa = 11.84SRR64 pKa = 11.84KK65 pKa = 8.8SKK67 pKa = 10.38RR68 pKa = 11.84RR69 pKa = 3.48

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

323

0

323

80165

33

1944

248.2

27.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.647 ± 0.16

1.094 ± 0.054

6.581 ± 0.152

6.84 ± 0.229

4.307 ± 0.081

5.943 ± 0.173

1.816 ± 0.056

6.14 ± 0.116

6.876 ± 0.242

8.229 ± 0.134

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.489 ± 0.055

4.82 ± 0.095

4.208 ± 0.086

3.393 ± 0.066

5.323 ± 0.109

6.977 ± 0.115

5.783 ± 0.144

6.957 ± 0.108

1.07 ± 0.046

3.508 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski