Amnibacterium setariae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Amnibacterium

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A1TTG2|A0A3A1TTG2_9MICO Tyrosine recombinase XerD OS=Amnibacterium setariae OX=2306585 GN=xerD PE=3 SV=1
MM1 pKa = 7.37LPVVGAVRR9 pKa = 11.84DD10 pKa = 3.95HH11 pKa = 6.47GAMSDD16 pKa = 3.95GEE18 pKa = 4.52DD19 pKa = 3.14EE20 pKa = 4.61GLRR23 pKa = 11.84GLHH26 pKa = 6.3PAVPVAADD34 pKa = 4.4DD35 pKa = 4.99RR36 pKa = 11.84DD37 pKa = 4.11LDD39 pKa = 4.08TPNCPDD45 pKa = 3.47CLVRR49 pKa = 11.84MEE51 pKa = 4.79AEE53 pKa = 3.99EE54 pKa = 4.32SAGGEE59 pKa = 4.28PYY61 pKa = 9.28WACTEE66 pKa = 4.2CGLVALAA73 pKa = 5.22

Molecular weight: 7.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A1TT91|A0A3A1TT91_9MICO GtrA family protein OS=Amnibacterium setariae OX=2306585 GN=D1781_16770 PE=3 SV=1
MM1 pKa = 7.8PPRR4 pKa = 11.84AAFQRR9 pKa = 11.84PRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84PDD15 pKa = 2.77PRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84PRR21 pKa = 11.84RR22 pKa = 11.84PPRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84AGLRR32 pKa = 11.84LRR34 pKa = 11.84SHH36 pKa = 7.6RR37 pKa = 11.84GRR39 pKa = 11.84SPPARR44 pKa = 11.84PPSRR48 pKa = 11.84LPRR51 pKa = 11.84PCRR54 pKa = 11.84SRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84SPRR61 pKa = 11.84RR62 pKa = 11.84SS63 pKa = 3.07

Molecular weight: 7.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3369

0

3369

1049265

27

1587

311.4

33.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.74 ± 0.074

0.438 ± 0.009

6.128 ± 0.037

5.414 ± 0.047

2.802 ± 0.028

9.33 ± 0.043

1.88 ± 0.022

3.491 ± 0.03

1.675 ± 0.029

10.609 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.452 ± 0.015

1.562 ± 0.02

5.842 ± 0.036

2.632 ± 0.026

8.456 ± 0.045

4.962 ± 0.037

5.723 ± 0.035

9.687 ± 0.046

1.452 ± 0.019

1.725 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski