Amino acid dipepetide frequency for Pseudomonas phage GP100

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.692AlaAla: 8.692 ± 1.394
0.778AlaCys: 0.778 ± 0.198
5.319AlaAsp: 5.319 ± 0.664
4.8AlaGlu: 4.8 ± 0.644
2.335AlaPhe: 2.335 ± 0.384
7.654AlaGly: 7.654 ± 0.818
1.751AlaHis: 1.751 ± 0.352
4.54AlaIle: 4.54 ± 0.474
6.551AlaLys: 6.551 ± 0.894
7.784AlaLeu: 7.784 ± 0.787
2.595AlaMet: 2.595 ± 0.452
3.762AlaAsn: 3.762 ± 0.439
4.086AlaPro: 4.086 ± 0.802
3.892AlaGln: 3.892 ± 0.567
4.476AlaArg: 4.476 ± 0.501
5.124AlaSer: 5.124 ± 0.636
4.994AlaThr: 4.994 ± 0.606
6.681AlaVal: 6.681 ± 0.735
1.362AlaTrp: 1.362 ± 0.319
2.595AlaTyr: 2.595 ± 0.314
0.0AlaXaa: 0.0 ± 0.0
Cys
0.778CysAla: 0.778 ± 0.273
0.259CysCys: 0.259 ± 0.116
0.454CysAsp: 0.454 ± 0.197
0.843CysGlu: 0.843 ± 0.288
0.454CysPhe: 0.454 ± 0.199
1.492CysGly: 1.492 ± 0.447
0.389CysHis: 0.389 ± 0.157
0.259CysIle: 0.259 ± 0.138
0.778CysLys: 0.778 ± 0.226
0.973CysLeu: 0.973 ± 0.282
0.389CysMet: 0.389 ± 0.219
0.649CysAsn: 0.649 ± 0.214
0.519CysPro: 0.519 ± 0.184
0.389CysGln: 0.389 ± 0.158
0.843CysArg: 0.843 ± 0.249
0.778CysSer: 0.778 ± 0.265
0.454CysThr: 0.454 ± 0.221
0.713CysVal: 0.713 ± 0.293
0.195CysTrp: 0.195 ± 0.12
0.259CysTyr: 0.259 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
5.513AspAla: 5.513 ± 0.664
0.713AspCys: 0.713 ± 0.25
4.93AspAsp: 4.93 ± 0.759
4.216AspGlu: 4.216 ± 0.613
2.919AspPhe: 2.919 ± 0.462
5.773AspGly: 5.773 ± 0.641
1.622AspHis: 1.622 ± 0.355
4.086AspIle: 4.086 ± 0.59
4.022AspLys: 4.022 ± 0.604
4.8AspLeu: 4.8 ± 0.498
1.751AspMet: 1.751 ± 0.482
2.854AspAsn: 2.854 ± 0.422
2.854AspPro: 2.854 ± 0.435
2.53AspGln: 2.53 ± 0.447
2.789AspArg: 2.789 ± 0.486
2.919AspSer: 2.919 ± 0.376
3.438AspThr: 3.438 ± 0.498
3.567AspVal: 3.567 ± 0.447
1.232AspTrp: 1.232 ± 0.317
3.373AspTyr: 3.373 ± 0.518
0.0AspXaa: 0.0 ± 0.0
Glu
5.578GluAla: 5.578 ± 0.552
0.973GluCys: 0.973 ± 0.31
5.254GluAsp: 5.254 ± 0.703
5.384GluGlu: 5.384 ± 0.761
3.243GluPhe: 3.243 ± 0.527
4.476GluGly: 4.476 ± 0.551
1.168GluHis: 1.168 ± 0.296
3.697GluIle: 3.697 ± 0.567
3.503GluLys: 3.503 ± 0.534
6.227GluLeu: 6.227 ± 0.951
1.232GluMet: 1.232 ± 0.342
1.362GluAsn: 1.362 ± 0.334
2.076GluPro: 2.076 ± 0.391
2.465GluGln: 2.465 ± 0.479
2.659GluArg: 2.659 ± 0.507
2.789GluSer: 2.789 ± 0.468
2.011GluThr: 2.011 ± 0.398
5.967GluVal: 5.967 ± 0.696
1.168GluTrp: 1.168 ± 0.251
2.53GluTyr: 2.53 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
3.049PheAla: 3.049 ± 0.395
0.908PheCys: 0.908 ± 0.258
3.049PheAsp: 3.049 ± 0.494
2.724PheGlu: 2.724 ± 0.51
1.103PhePhe: 1.103 ± 0.278
3.049PheGly: 3.049 ± 0.393
0.649PheHis: 0.649 ± 0.186
2.14PheIle: 2.14 ± 0.399
3.243PheLys: 3.243 ± 0.498
2.465PheLeu: 2.465 ± 0.41
1.038PheMet: 1.038 ± 0.227
1.881PheAsn: 1.881 ± 0.305
1.232PhePro: 1.232 ± 0.259
1.103PheGln: 1.103 ± 0.254
1.751PheArg: 1.751 ± 0.315
1.881PheSer: 1.881 ± 0.356
2.27PheThr: 2.27 ± 0.376
2.659PheVal: 2.659 ± 0.414
0.778PheTrp: 0.778 ± 0.224
1.232PheTyr: 1.232 ± 0.253
0.0PheXaa: 0.0 ± 0.0
Gly
5.903GlyAla: 5.903 ± 0.721
0.908GlyCys: 0.908 ± 0.331
5.449GlyAsp: 5.449 ± 0.534
4.54GlyGlu: 4.54 ± 0.595
3.178GlyPhe: 3.178 ± 0.408
5.838GlyGly: 5.838 ± 0.709
1.557GlyHis: 1.557 ± 0.329
3.308GlyIle: 3.308 ± 0.415
5.449GlyLys: 5.449 ± 0.792
6.811GlyLeu: 6.811 ± 0.776
1.751GlyMet: 1.751 ± 0.371
3.438GlyAsn: 3.438 ± 0.512
1.816GlyPro: 1.816 ± 0.382
2.076GlyGln: 2.076 ± 0.414
3.632GlyArg: 3.632 ± 0.354
4.735GlySer: 4.735 ± 0.92
4.605GlyThr: 4.605 ± 0.912
5.903GlyVal: 5.903 ± 0.691
1.686GlyTrp: 1.686 ± 0.353
2.789GlyTyr: 2.789 ± 0.386
0.0GlyXaa: 0.0 ± 0.0
His
0.973HisAla: 0.973 ± 0.27
0.259HisCys: 0.259 ± 0.162
1.362HisAsp: 1.362 ± 0.303
1.168HisGlu: 1.168 ± 0.306
1.103HisPhe: 1.103 ± 0.315
1.686HisGly: 1.686 ± 0.377
0.454HisHis: 0.454 ± 0.214
1.362HisIle: 1.362 ± 0.313
1.427HisLys: 1.427 ± 0.283
1.946HisLeu: 1.946 ± 0.336
0.389HisMet: 0.389 ± 0.162
0.713HisAsn: 0.713 ± 0.215
0.908HisPro: 0.908 ± 0.293
0.843HisGln: 0.843 ± 0.226
0.843HisArg: 0.843 ± 0.274
1.362HisSer: 1.362 ± 0.283
0.454HisThr: 0.454 ± 0.151
1.881HisVal: 1.881 ± 0.482
0.324HisTrp: 0.324 ± 0.154
0.649HisTyr: 0.649 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.151IleAla: 4.151 ± 0.512
0.454IleCys: 0.454 ± 0.23
4.476IleAsp: 4.476 ± 0.466
3.178IleGlu: 3.178 ± 0.594
2.14IlePhe: 2.14 ± 0.391
3.503IleGly: 3.503 ± 0.481
1.038IleHis: 1.038 ± 0.197
2.205IleIle: 2.205 ± 0.366
3.567IleLys: 3.567 ± 0.486
3.308IleLeu: 3.308 ± 0.47
1.103IleMet: 1.103 ± 0.28
2.659IleAsn: 2.659 ± 0.603
2.27IlePro: 2.27 ± 0.373
2.919IleGln: 2.919 ± 0.432
3.243IleArg: 3.243 ± 0.583
2.27IleSer: 2.27 ± 0.464
3.178IleThr: 3.178 ± 0.546
3.632IleVal: 3.632 ± 0.492
0.389IleTrp: 0.389 ± 0.149
2.205IleTyr: 2.205 ± 0.374
0.0IleXaa: 0.0 ± 0.0
Lys
7.589LysAla: 7.589 ± 1.001
0.778LysCys: 0.778 ± 0.208
4.994LysAsp: 4.994 ± 0.81
4.346LysGlu: 4.346 ± 0.593
2.205LysPhe: 2.205 ± 0.324
4.216LysGly: 4.216 ± 0.724
1.362LysHis: 1.362 ± 0.298
3.113LysIle: 3.113 ± 0.529
4.086LysLys: 4.086 ± 0.763
4.93LysLeu: 4.93 ± 0.588
1.362LysMet: 1.362 ± 0.32
2.205LysAsn: 2.205 ± 0.351
2.789LysPro: 2.789 ± 0.517
2.4LysGln: 2.4 ± 0.485
3.113LysArg: 3.113 ± 0.55
2.919LysSer: 2.919 ± 0.532
3.243LysThr: 3.243 ± 0.47
5.124LysVal: 5.124 ± 0.609
0.908LysTrp: 0.908 ± 0.245
2.14LysTyr: 2.14 ± 0.334
0.0LysXaa: 0.0 ± 0.0
Leu
7.784LeuAla: 7.784 ± 0.756
1.038LeuCys: 1.038 ± 0.287
4.8LeuAsp: 4.8 ± 0.466
6.486LeuGlu: 6.486 ± 0.792
2.854LeuPhe: 2.854 ± 0.423
5.838LeuGly: 5.838 ± 0.516
1.103LeuHis: 1.103 ± 0.269
3.632LeuIle: 3.632 ± 0.478
4.605LeuLys: 4.605 ± 0.75
6.357LeuLeu: 6.357 ± 0.639
1.427LeuMet: 1.427 ± 0.352
3.438LeuAsn: 3.438 ± 0.449
3.762LeuPro: 3.762 ± 0.489
2.595LeuGln: 2.595 ± 0.409
5.059LeuArg: 5.059 ± 0.571
4.8LeuSer: 4.8 ± 0.531
6.162LeuThr: 6.162 ± 0.644
6.097LeuVal: 6.097 ± 0.636
1.427LeuTrp: 1.427 ± 0.387
3.308LeuTyr: 3.308 ± 0.428
0.0LeuXaa: 0.0 ± 0.0
Met
2.659MetAla: 2.659 ± 0.399
0.324MetCys: 0.324 ± 0.172
1.297MetAsp: 1.297 ± 0.3
1.038MetGlu: 1.038 ± 0.277
0.908MetPhe: 0.908 ± 0.246
1.622MetGly: 1.622 ± 0.316
0.389MetHis: 0.389 ± 0.151
1.168MetIle: 1.168 ± 0.297
1.686MetLys: 1.686 ± 0.405
1.622MetLeu: 1.622 ± 0.318
0.584MetMet: 0.584 ± 0.169
1.168MetAsn: 1.168 ± 0.26
1.103MetPro: 1.103 ± 0.265
0.973MetGln: 0.973 ± 0.246
1.168MetArg: 1.168 ± 0.284
2.011MetSer: 2.011 ± 0.336
1.427MetThr: 1.427 ± 0.332
1.038MetVal: 1.038 ± 0.263
0.324MetTrp: 0.324 ± 0.17
1.232MetTyr: 1.232 ± 0.234
0.0MetXaa: 0.0 ± 0.0
Asn
3.892AsnAla: 3.892 ± 0.53
0.454AsnCys: 0.454 ± 0.207
2.076AsnAsp: 2.076 ± 0.366
1.816AsnGlu: 1.816 ± 0.372
1.427AsnPhe: 1.427 ± 0.297
3.373AsnGly: 3.373 ± 0.566
0.713AsnHis: 0.713 ± 0.207
3.243AsnIle: 3.243 ± 0.597
2.335AsnLys: 2.335 ± 0.373
4.281AsnLeu: 4.281 ± 0.559
0.843AsnMet: 0.843 ± 0.243
1.946AsnAsn: 1.946 ± 0.321
2.724AsnPro: 2.724 ± 0.498
2.4AsnGln: 2.4 ± 0.539
2.27AsnArg: 2.27 ± 0.339
2.789AsnSer: 2.789 ± 0.512
2.27AsnThr: 2.27 ± 0.326
3.373AsnVal: 3.373 ± 0.494
0.973AsnTrp: 0.973 ± 0.257
1.427AsnTyr: 1.427 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
3.567ProAla: 3.567 ± 0.614
0.195ProCys: 0.195 ± 0.115
2.465ProAsp: 2.465 ± 0.474
3.503ProGlu: 3.503 ± 0.519
0.843ProPhe: 0.843 ± 0.219
2.659ProGly: 2.659 ± 0.365
1.103ProHis: 1.103 ± 0.289
2.27ProIle: 2.27 ± 0.369
2.465ProLys: 2.465 ± 0.696
3.243ProLeu: 3.243 ± 0.461
0.778ProMet: 0.778 ± 0.221
1.946ProAsn: 1.946 ± 0.27
1.622ProPro: 1.622 ± 0.353
1.492ProGln: 1.492 ± 0.296
1.362ProArg: 1.362 ± 0.318
2.27ProSer: 2.27 ± 0.548
2.595ProThr: 2.595 ± 0.6
3.178ProVal: 3.178 ± 0.529
0.584ProTrp: 0.584 ± 0.216
1.232ProTyr: 1.232 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
4.476GlnAla: 4.476 ± 0.659
0.454GlnCys: 0.454 ± 0.162
2.465GlnAsp: 2.465 ± 0.426
3.438GlnGlu: 3.438 ± 0.532
1.946GlnPhe: 1.946 ± 0.327
2.595GlnGly: 2.595 ± 0.367
1.103GlnHis: 1.103 ± 0.254
2.011GlnIle: 2.011 ± 0.434
2.205GlnLys: 2.205 ± 0.498
3.373GlnLeu: 3.373 ± 0.376
1.103GlnMet: 1.103 ± 0.3
1.492GlnAsn: 1.492 ± 0.373
1.168GlnPro: 1.168 ± 0.315
2.595GlnGln: 2.595 ± 0.731
2.27GlnArg: 2.27 ± 0.406
2.14GlnSer: 2.14 ± 0.369
1.492GlnThr: 1.492 ± 0.355
2.984GlnVal: 2.984 ± 0.385
0.389GlnTrp: 0.389 ± 0.118
1.492GlnTyr: 1.492 ± 0.269
0.0GlnXaa: 0.0 ± 0.0
Arg
5.578ArgAla: 5.578 ± 0.614
0.713ArgCys: 0.713 ± 0.24
3.243ArgAsp: 3.243 ± 0.499
2.919ArgGlu: 2.919 ± 0.536
1.881ArgPhe: 1.881 ± 0.451
3.827ArgGly: 3.827 ± 0.522
1.168ArgHis: 1.168 ± 0.317
1.622ArgIle: 1.622 ± 0.319
3.113ArgLys: 3.113 ± 0.475
4.216ArgLeu: 4.216 ± 0.633
1.297ArgMet: 1.297 ± 0.357
2.595ArgAsn: 2.595 ± 0.464
1.557ArgPro: 1.557 ± 0.359
1.816ArgGln: 1.816 ± 0.35
3.113ArgArg: 3.113 ± 0.575
2.465ArgSer: 2.465 ± 0.442
2.465ArgThr: 2.465 ± 0.447
3.373ArgVal: 3.373 ± 0.527
1.232ArgTrp: 1.232 ± 0.263
1.103ArgTyr: 1.103 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
5.059SerAla: 5.059 ± 0.802
0.649SerCys: 0.649 ± 0.257
2.27SerAsp: 2.27 ± 0.476
2.854SerGlu: 2.854 ± 0.534
2.4SerPhe: 2.4 ± 0.43
4.086SerGly: 4.086 ± 0.589
1.038SerHis: 1.038 ± 0.227
3.373SerIle: 3.373 ± 0.401
4.022SerLys: 4.022 ± 0.622
4.93SerLeu: 4.93 ± 0.491
1.038SerMet: 1.038 ± 0.279
2.724SerAsn: 2.724 ± 0.455
2.465SerPro: 2.465 ± 0.51
3.049SerGln: 3.049 ± 0.599
2.4SerArg: 2.4 ± 0.363
2.984SerSer: 2.984 ± 0.556
3.373SerThr: 3.373 ± 0.55
4.346SerVal: 4.346 ± 0.578
0.519SerTrp: 0.519 ± 0.161
2.011SerTyr: 2.011 ± 0.408
0.0SerXaa: 0.0 ± 0.0
Thr
3.632ThrAla: 3.632 ± 0.711
0.584ThrCys: 0.584 ± 0.191
3.373ThrAsp: 3.373 ± 0.678
2.465ThrGlu: 2.465 ± 0.33
2.335ThrPhe: 2.335 ± 0.583
4.605ThrGly: 4.605 ± 0.737
0.908ThrHis: 0.908 ± 0.232
3.892ThrIle: 3.892 ± 0.741
2.984ThrLys: 2.984 ± 0.507
4.605ThrLeu: 4.605 ± 0.637
1.557ThrMet: 1.557 ± 0.379
2.724ThrAsn: 2.724 ± 0.39
2.14ThrPro: 2.14 ± 0.327
2.465ThrGln: 2.465 ± 0.444
2.27ThrArg: 2.27 ± 0.426
3.503ThrSer: 3.503 ± 0.702
4.086ThrThr: 4.086 ± 0.744
4.151ThrVal: 4.151 ± 0.603
1.232ThrTrp: 1.232 ± 0.274
2.4ThrTyr: 2.4 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
6.811ValAla: 6.811 ± 0.821
0.778ValCys: 0.778 ± 0.196
4.865ValAsp: 4.865 ± 0.544
4.8ValGlu: 4.8 ± 0.616
2.984ValPhe: 2.984 ± 0.391
5.124ValGly: 5.124 ± 0.709
1.557ValHis: 1.557 ± 0.278
3.503ValIle: 3.503 ± 0.565
4.086ValLys: 4.086 ± 0.67
6.746ValLeu: 6.746 ± 0.748
1.622ValMet: 1.622 ± 0.338
3.567ValAsn: 3.567 ± 0.553
2.659ValPro: 2.659 ± 0.583
3.113ValGln: 3.113 ± 0.438
3.697ValArg: 3.697 ± 0.451
4.476ValSer: 4.476 ± 0.618
4.735ValThr: 4.735 ± 0.92
6.551ValVal: 6.551 ± 0.843
0.649ValTrp: 0.649 ± 0.212
2.465ValTyr: 2.465 ± 0.344
0.0ValXaa: 0.0 ± 0.0
Trp
1.362TrpAla: 1.362 ± 0.396
0.195TrpCys: 0.195 ± 0.113
1.168TrpAsp: 1.168 ± 0.29
1.103TrpGlu: 1.103 ± 0.311
0.778TrpPhe: 0.778 ± 0.219
0.649TrpGly: 0.649 ± 0.186
0.324TrpHis: 0.324 ± 0.169
0.649TrpIle: 0.649 ± 0.202
0.973TrpLys: 0.973 ± 0.257
1.168TrpLeu: 1.168 ± 0.262
0.843TrpMet: 0.843 ± 0.252
0.843TrpAsn: 0.843 ± 0.189
0.454TrpPro: 0.454 ± 0.196
0.519TrpGln: 0.519 ± 0.153
0.973TrpArg: 0.973 ± 0.233
1.557TrpSer: 1.557 ± 0.303
0.778TrpThr: 0.778 ± 0.232
0.778TrpVal: 0.778 ± 0.236
0.324TrpTrp: 0.324 ± 0.138
0.713TrpTyr: 0.713 ± 0.228
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.724TyrAla: 2.724 ± 0.464
0.454TyrCys: 0.454 ± 0.171
2.595TyrAsp: 2.595 ± 0.378
2.14TyrGlu: 2.14 ± 0.35
1.168TyrPhe: 1.168 ± 0.25
3.308TyrGly: 3.308 ± 0.522
0.778TyrHis: 0.778 ± 0.244
1.816TyrIle: 1.816 ± 0.411
2.724TyrLys: 2.724 ± 0.397
2.789TyrLeu: 2.789 ± 0.461
0.973TyrMet: 0.973 ± 0.199
2.659TyrAsn: 2.659 ± 0.363
1.168TyrPro: 1.168 ± 0.212
1.557TyrGln: 1.557 ± 0.333
1.362TyrArg: 1.362 ± 0.312
1.816TyrSer: 1.816 ± 0.378
1.881TyrThr: 1.881 ± 0.347
2.789TyrVal: 2.789 ± 0.501
0.454TyrTrp: 0.454 ± 0.138
0.908TyrTyr: 0.908 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (15418 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski