Amino acid dipepetide frequency for Mycobacterium phage Francis47

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.832AlaAla: 13.832 ± 1.11
0.719AlaCys: 0.719 ± 0.2
7.066AlaAsp: 7.066 ± 0.722
6.228AlaGlu: 6.228 ± 0.77
2.934AlaPhe: 2.934 ± 0.506
8.323AlaGly: 8.323 ± 0.866
1.557AlaHis: 1.557 ± 0.364
3.892AlaIle: 3.892 ± 0.519
4.371AlaLys: 4.371 ± 0.531
8.683AlaLeu: 8.683 ± 0.823
2.754AlaMet: 2.754 ± 0.515
2.754AlaAsn: 2.754 ± 0.454
4.85AlaPro: 4.85 ± 0.634
2.874AlaGln: 2.874 ± 0.44
5.868AlaArg: 5.868 ± 0.531
4.97AlaSer: 4.97 ± 0.608
6.048AlaThr: 6.048 ± 0.702
8.323AlaVal: 8.323 ± 0.776
2.096AlaTrp: 2.096 ± 0.434
2.754AlaTyr: 2.754 ± 0.35
0.0AlaXaa: 0.0 ± 0.0
Cys
0.599CysAla: 0.599 ± 0.2
0.0CysCys: 0.0 ± 0.0
0.479CysAsp: 0.479 ± 0.157
0.898CysGlu: 0.898 ± 0.305
0.12CysPhe: 0.12 ± 0.076
0.958CysGly: 0.958 ± 0.287
0.06CysHis: 0.06 ± 0.07
0.18CysIle: 0.18 ± 0.119
0.24CysLys: 0.24 ± 0.117
0.479CysLeu: 0.479 ± 0.199
0.12CysMet: 0.12 ± 0.082
0.24CysAsn: 0.24 ± 0.127
0.419CysPro: 0.419 ± 0.149
0.24CysGln: 0.24 ± 0.116
0.359CysArg: 0.359 ± 0.143
0.359CysSer: 0.359 ± 0.15
0.479CysThr: 0.479 ± 0.177
0.359CysVal: 0.359 ± 0.134
0.24CysTrp: 0.24 ± 0.126
0.12CysTyr: 0.12 ± 0.089
0.0CysXaa: 0.0 ± 0.0
Asp
6.287AspAla: 6.287 ± 0.575
0.599AspCys: 0.599 ± 0.179
4.97AspAsp: 4.97 ± 0.526
3.593AspGlu: 3.593 ± 0.48
2.695AspPhe: 2.695 ± 0.354
5.868AspGly: 5.868 ± 0.648
1.497AspHis: 1.497 ± 0.341
2.216AspIle: 2.216 ± 0.429
2.575AspLys: 2.575 ± 0.41
7.365AspLeu: 7.365 ± 0.79
1.257AspMet: 1.257 ± 0.232
1.916AspAsn: 1.916 ± 0.335
4.79AspPro: 4.79 ± 0.634
1.617AspGln: 1.617 ± 0.371
3.653AspArg: 3.653 ± 0.43
3.413AspSer: 3.413 ± 0.468
3.832AspThr: 3.832 ± 0.41
5.15AspVal: 5.15 ± 0.659
1.737AspTrp: 1.737 ± 0.293
2.036AspTyr: 2.036 ± 0.371
0.0AspXaa: 0.0 ± 0.0
Glu
6.527GluAla: 6.527 ± 0.838
0.24GluCys: 0.24 ± 0.148
4.731GluAsp: 4.731 ± 0.442
4.611GluGlu: 4.611 ± 0.593
1.916GluPhe: 1.916 ± 0.356
3.892GluGly: 3.892 ± 0.442
1.257GluHis: 1.257 ± 0.306
3.653GluIle: 3.653 ± 0.408
2.395GluLys: 2.395 ± 0.39
6.467GluLeu: 6.467 ± 0.541
1.557GluMet: 1.557 ± 0.307
1.617GluAsn: 1.617 ± 0.339
2.455GluPro: 2.455 ± 0.394
3.114GluGln: 3.114 ± 0.408
3.593GluArg: 3.593 ± 0.445
3.533GluSer: 3.533 ± 0.441
4.012GluThr: 4.012 ± 0.489
5.509GluVal: 5.509 ± 0.564
1.377GluTrp: 1.377 ± 0.351
2.695GluTyr: 2.695 ± 0.457
0.0GluXaa: 0.0 ± 0.0
Phe
2.515PheAla: 2.515 ± 0.373
0.419PheCys: 0.419 ± 0.157
2.874PheAsp: 2.874 ± 0.332
1.976PheGlu: 1.976 ± 0.372
0.539PhePhe: 0.539 ± 0.157
3.593PheGly: 3.593 ± 0.42
0.659PheHis: 0.659 ± 0.261
1.497PheIle: 1.497 ± 0.25
1.317PheLys: 1.317 ± 0.24
2.335PheLeu: 2.335 ± 0.413
0.539PheMet: 0.539 ± 0.254
1.677PheAsn: 1.677 ± 0.295
1.617PhePro: 1.617 ± 0.31
0.719PheGln: 0.719 ± 0.165
1.557PheArg: 1.557 ± 0.351
1.737PheSer: 1.737 ± 0.252
2.216PheThr: 2.216 ± 0.392
1.916PheVal: 1.916 ± 0.359
0.539PheTrp: 0.539 ± 0.145
1.018PheTyr: 1.018 ± 0.26
0.0PheXaa: 0.0 ± 0.0
Gly
6.647GlyAla: 6.647 ± 0.894
0.539GlyCys: 0.539 ± 0.168
6.228GlyAsp: 6.228 ± 0.62
4.371GlyGlu: 4.371 ± 0.525
2.635GlyPhe: 2.635 ± 0.476
9.521GlyGly: 9.521 ± 2.384
1.737GlyHis: 1.737 ± 0.34
4.012GlyIle: 4.012 ± 0.682
4.012GlyLys: 4.012 ± 0.483
8.024GlyLeu: 8.024 ± 0.612
2.036GlyMet: 2.036 ± 0.33
3.174GlyAsn: 3.174 ± 0.452
4.132GlyPro: 4.132 ± 0.638
2.156GlyGln: 2.156 ± 0.37
5.15GlyArg: 5.15 ± 0.621
6.048GlySer: 6.048 ± 0.815
5.269GlyThr: 5.269 ± 0.549
5.269GlyVal: 5.269 ± 0.583
2.275GlyTrp: 2.275 ± 0.353
2.934GlyTyr: 2.934 ± 0.4
0.0GlyXaa: 0.0 ± 0.0
His
1.856HisAla: 1.856 ± 0.404
0.12HisCys: 0.12 ± 0.118
1.257HisAsp: 1.257 ± 0.228
1.497HisGlu: 1.497 ± 0.328
0.778HisPhe: 0.778 ± 0.227
1.617HisGly: 1.617 ± 0.403
0.659HisHis: 0.659 ± 0.206
1.018HisIle: 1.018 ± 0.215
1.138HisLys: 1.138 ± 0.286
1.317HisLeu: 1.317 ± 0.318
0.18HisMet: 0.18 ± 0.093
0.18HisAsn: 0.18 ± 0.108
1.257HisPro: 1.257 ± 0.235
1.018HisGln: 1.018 ± 0.23
1.497HisArg: 1.497 ± 0.302
0.659HisSer: 0.659 ± 0.202
1.198HisThr: 1.198 ± 0.292
1.617HisVal: 1.617 ± 0.315
0.539HisTrp: 0.539 ± 0.148
0.838HisTyr: 0.838 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
6.407IleAla: 6.407 ± 0.547
0.24IleCys: 0.24 ± 0.127
3.593IleAsp: 3.593 ± 0.445
3.772IleGlu: 3.772 ± 0.486
0.719IlePhe: 0.719 ± 0.239
3.952IleGly: 3.952 ± 0.449
0.958IleHis: 0.958 ± 0.239
1.796IleIle: 1.796 ± 0.308
1.737IleLys: 1.737 ± 0.349
3.413IleLeu: 3.413 ± 0.378
0.719IleMet: 0.719 ± 0.204
1.856IleAsn: 1.856 ± 0.312
2.874IlePro: 2.874 ± 0.391
1.257IleGln: 1.257 ± 0.309
3.054IleArg: 3.054 ± 0.377
2.874IleSer: 2.874 ± 0.4
3.593IleThr: 3.593 ± 0.439
2.635IleVal: 2.635 ± 0.472
0.778IleTrp: 0.778 ± 0.192
1.437IleTyr: 1.437 ± 0.244
0.0IleXaa: 0.0 ± 0.0
Lys
3.772LysAla: 3.772 ± 0.523
0.359LysCys: 0.359 ± 0.148
2.635LysAsp: 2.635 ± 0.523
2.096LysGlu: 2.096 ± 0.392
1.557LysPhe: 1.557 ± 0.296
2.575LysGly: 2.575 ± 0.398
0.958LysHis: 0.958 ± 0.288
2.275LysIle: 2.275 ± 0.381
2.096LysLys: 2.096 ± 0.462
3.353LysLeu: 3.353 ± 0.384
1.198LysMet: 1.198 ± 0.235
1.317LysAsn: 1.317 ± 0.266
2.635LysPro: 2.635 ± 0.477
1.737LysGln: 1.737 ± 0.424
2.814LysArg: 2.814 ± 0.502
2.515LysSer: 2.515 ± 0.383
2.395LysThr: 2.395 ± 0.402
3.593LysVal: 3.593 ± 0.499
0.719LysTrp: 0.719 ± 0.221
1.078LysTyr: 1.078 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
8.862LeuAla: 8.862 ± 0.796
0.24LeuCys: 0.24 ± 0.117
6.048LeuAsp: 6.048 ± 0.574
5.569LeuGlu: 5.569 ± 0.652
2.275LeuPhe: 2.275 ± 0.339
7.186LeuGly: 7.186 ± 0.701
1.856LeuHis: 1.856 ± 0.346
3.952LeuIle: 3.952 ± 0.473
3.952LeuLys: 3.952 ± 0.476
5.749LeuLeu: 5.749 ± 0.617
1.677LeuMet: 1.677 ± 0.266
3.054LeuAsn: 3.054 ± 0.403
5.509LeuPro: 5.509 ± 0.602
2.575LeuGln: 2.575 ± 0.374
6.168LeuArg: 6.168 ± 0.519
5.749LeuSer: 5.749 ± 0.533
6.228LeuThr: 6.228 ± 0.538
4.671LeuVal: 4.671 ± 0.606
0.958LeuTrp: 0.958 ± 0.33
2.575LeuTyr: 2.575 ± 0.407
0.0LeuXaa: 0.0 ± 0.0
Met
2.635MetAla: 2.635 ± 0.399
0.0MetCys: 0.0 ± 0.0
0.898MetAsp: 0.898 ± 0.224
1.377MetGlu: 1.377 ± 0.284
0.838MetPhe: 0.838 ± 0.198
1.198MetGly: 1.198 ± 0.25
0.24MetHis: 0.24 ± 0.129
1.198MetIle: 1.198 ± 0.263
1.138MetLys: 1.138 ± 0.251
1.138MetLeu: 1.138 ± 0.245
0.06MetMet: 0.06 ± 0.063
0.958MetAsn: 0.958 ± 0.204
0.898MetPro: 0.898 ± 0.203
0.539MetGln: 0.539 ± 0.159
1.138MetArg: 1.138 ± 0.25
2.096MetSer: 2.096 ± 0.363
2.096MetThr: 2.096 ± 0.29
1.497MetVal: 1.497 ± 0.33
0.359MetTrp: 0.359 ± 0.14
0.359MetTyr: 0.359 ± 0.132
0.0MetXaa: 0.0 ± 0.0
Asn
3.413AsnAla: 3.413 ± 0.466
0.12AsnCys: 0.12 ± 0.085
1.856AsnAsp: 1.856 ± 0.355
2.275AsnGlu: 2.275 ± 0.375
0.958AsnPhe: 0.958 ± 0.28
3.653AsnGly: 3.653 ± 0.487
0.838AsnHis: 0.838 ± 0.219
1.856AsnIle: 1.856 ± 0.356
0.659AsnLys: 0.659 ± 0.212
2.515AsnLeu: 2.515 ± 0.326
0.539AsnMet: 0.539 ± 0.156
1.078AsnAsn: 1.078 ± 0.239
2.575AsnPro: 2.575 ± 0.395
0.898AsnGln: 0.898 ± 0.219
1.437AsnArg: 1.437 ± 0.328
1.856AsnSer: 1.856 ± 0.342
2.275AsnThr: 2.275 ± 0.443
2.455AsnVal: 2.455 ± 0.413
0.838AsnTrp: 0.838 ± 0.197
1.018AsnTyr: 1.018 ± 0.243
0.0AsnXaa: 0.0 ± 0.0
Pro
4.551ProAla: 4.551 ± 0.537
0.539ProCys: 0.539 ± 0.19
4.491ProAsp: 4.491 ± 0.521
4.311ProGlu: 4.311 ± 0.539
2.096ProPhe: 2.096 ± 0.382
4.431ProGly: 4.431 ± 0.608
0.898ProHis: 0.898 ± 0.219
2.216ProIle: 2.216 ± 0.374
2.216ProLys: 2.216 ± 0.282
4.192ProLeu: 4.192 ± 0.544
1.138ProMet: 1.138 ± 0.296
1.737ProAsn: 1.737 ± 0.335
2.934ProPro: 2.934 ± 0.47
1.617ProGln: 1.617 ± 0.352
2.455ProArg: 2.455 ± 0.417
3.653ProSer: 3.653 ± 0.395
3.772ProThr: 3.772 ± 0.595
3.772ProVal: 3.772 ± 0.488
0.898ProTrp: 0.898 ± 0.318
1.737ProTyr: 1.737 ± 0.4
0.0ProXaa: 0.0 ± 0.0
Gln
3.174GlnAla: 3.174 ± 0.564
0.12GlnCys: 0.12 ± 0.087
1.257GlnAsp: 1.257 ± 0.341
1.557GlnGlu: 1.557 ± 0.267
0.898GlnPhe: 0.898 ± 0.222
2.754GlnGly: 2.754 ± 0.393
0.719GlnHis: 0.719 ± 0.176
2.754GlnIle: 2.754 ± 0.549
1.198GlnLys: 1.198 ± 0.297
3.593GlnLeu: 3.593 ± 0.438
0.898GlnMet: 0.898 ± 0.257
0.419GlnAsn: 0.419 ± 0.144
1.796GlnPro: 1.796 ± 0.297
1.796GlnGln: 1.796 ± 0.311
1.796GlnArg: 1.796 ± 0.29
1.916GlnSer: 1.916 ± 0.309
1.976GlnThr: 1.976 ± 0.357
2.275GlnVal: 2.275 ± 0.304
0.659GlnTrp: 0.659 ± 0.17
0.659GlnTyr: 0.659 ± 0.186
0.0GlnXaa: 0.0 ± 0.0
Arg
5.449ArgAla: 5.449 ± 0.63
0.778ArgCys: 0.778 ± 0.288
2.934ArgAsp: 2.934 ± 0.393
4.311ArgGlu: 4.311 ± 0.571
1.856ArgPhe: 1.856 ± 0.341
4.97ArgGly: 4.97 ± 0.598
1.138ArgHis: 1.138 ± 0.266
3.174ArgIle: 3.174 ± 0.512
3.174ArgLys: 3.174 ± 0.492
5.928ArgLeu: 5.928 ± 0.687
1.677ArgMet: 1.677 ± 0.306
2.335ArgAsn: 2.335 ± 0.424
2.096ArgPro: 2.096 ± 0.383
1.976ArgGln: 1.976 ± 0.338
5.15ArgArg: 5.15 ± 0.667
3.473ArgSer: 3.473 ± 0.498
2.754ArgThr: 2.754 ± 0.485
4.671ArgVal: 4.671 ± 0.47
1.317ArgTrp: 1.317 ± 0.267
1.796ArgTyr: 1.796 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
6.527SerAla: 6.527 ± 0.75
0.599SerCys: 0.599 ± 0.174
3.353SerAsp: 3.353 ± 0.489
3.952SerGlu: 3.952 ± 0.474
2.036SerPhe: 2.036 ± 0.362
6.108SerGly: 6.108 ± 0.787
1.437SerHis: 1.437 ± 0.294
2.575SerIle: 2.575 ± 0.415
2.275SerLys: 2.275 ± 0.384
4.671SerLeu: 4.671 ± 0.609
1.677SerMet: 1.677 ± 0.352
2.635SerAsn: 2.635 ± 0.461
2.934SerPro: 2.934 ± 0.497
1.796SerGln: 1.796 ± 0.286
3.114SerArg: 3.114 ± 0.424
3.174SerSer: 3.174 ± 0.685
3.353SerThr: 3.353 ± 0.483
4.072SerVal: 4.072 ± 0.535
1.317SerTrp: 1.317 ± 0.339
1.737SerTyr: 1.737 ± 0.302
0.0SerXaa: 0.0 ± 0.0
Thr
6.407ThrAla: 6.407 ± 0.629
0.419ThrCys: 0.419 ± 0.178
4.311ThrAsp: 4.311 ± 0.554
4.671ThrGlu: 4.671 ± 0.54
2.216ThrPhe: 2.216 ± 0.388
6.527ThrGly: 6.527 ± 0.601
1.078ThrHis: 1.078 ± 0.352
2.515ThrIle: 2.515 ± 0.544
2.275ThrLys: 2.275 ± 0.334
6.228ThrLeu: 6.228 ± 0.622
0.778ThrMet: 0.778 ± 0.217
1.737ThrAsn: 1.737 ± 0.367
3.832ThrPro: 3.832 ± 0.52
1.796ThrGln: 1.796 ± 0.368
3.234ThrArg: 3.234 ± 0.516
4.072ThrSer: 4.072 ± 0.496
4.132ThrThr: 4.132 ± 0.547
5.928ThrVal: 5.928 ± 0.669
1.078ThrTrp: 1.078 ± 0.237
1.856ThrTyr: 1.856 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
7.126ValAla: 7.126 ± 0.792
0.359ValCys: 0.359 ± 0.14
5.15ValAsp: 5.15 ± 0.488
4.731ValGlu: 4.731 ± 0.563
2.455ValPhe: 2.455 ± 0.397
4.85ValGly: 4.85 ± 0.666
1.497ValHis: 1.497 ± 0.257
4.072ValIle: 4.072 ± 0.496
3.234ValLys: 3.234 ± 0.516
4.91ValLeu: 4.91 ± 0.533
0.898ValMet: 0.898 ± 0.309
2.335ValAsn: 2.335 ± 0.382
4.132ValPro: 4.132 ± 0.474
2.395ValGln: 2.395 ± 0.345
5.15ValArg: 5.15 ± 0.594
4.551ValSer: 4.551 ± 0.389
5.509ValThr: 5.509 ± 0.503
5.03ValVal: 5.03 ± 0.625
1.737ValTrp: 1.737 ± 0.376
2.515ValTyr: 2.515 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
1.437TrpAla: 1.437 ± 0.293
0.24TrpCys: 0.24 ± 0.103
1.497TrpAsp: 1.497 ± 0.323
0.898TrpGlu: 0.898 ± 0.201
1.018TrpPhe: 1.018 ± 0.254
1.497TrpGly: 1.497 ± 0.271
0.419TrpHis: 0.419 ± 0.148
1.317TrpIle: 1.317 ± 0.25
0.299TrpLys: 0.299 ± 0.134
1.796TrpLeu: 1.796 ± 0.372
0.359TrpMet: 0.359 ± 0.176
0.778TrpAsn: 0.778 ± 0.232
0.778TrpPro: 0.778 ± 0.281
0.898TrpGln: 0.898 ± 0.242
1.317TrpArg: 1.317 ± 0.313
1.138TrpSer: 1.138 ± 0.263
1.856TrpThr: 1.856 ± 0.432
1.916TrpVal: 1.916 ± 0.33
0.659TrpTrp: 0.659 ± 0.28
0.359TrpTyr: 0.359 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.874TyrAla: 2.874 ± 0.402
0.299TyrCys: 0.299 ± 0.178
1.437TyrAsp: 1.437 ± 0.276
2.455TyrGlu: 2.455 ± 0.398
0.719TyrPhe: 0.719 ± 0.227
2.695TyrGly: 2.695 ± 0.421
0.778TyrHis: 0.778 ± 0.252
1.497TyrIle: 1.497 ± 0.35
1.377TyrLys: 1.377 ± 0.294
2.695TyrLeu: 2.695 ± 0.381
0.539TyrMet: 0.539 ± 0.169
1.317TyrAsn: 1.317 ± 0.334
1.138TyrPro: 1.138 ± 0.267
1.198TyrGln: 1.198 ± 0.286
2.515TyrArg: 2.515 ± 0.438
1.557TyrSer: 1.557 ± 0.282
2.096TyrThr: 2.096 ± 0.43
1.976TyrVal: 1.976 ± 0.359
0.359TyrTrp: 0.359 ± 0.135
0.419TyrTyr: 0.419 ± 0.158
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 93 proteins (16701 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski