Salmonella phage vB_SenS_SB28

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TA29|A0A5J6TA29_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SB28 OX=2591136 PE=4 SV=1
MM1 pKa = 7.52SEE3 pKa = 3.88NPANLVCVKK12 pKa = 10.82SKK14 pKa = 10.84IPWFKK19 pKa = 10.85DD20 pKa = 2.65GCSYY24 pKa = 9.49ITYY27 pKa = 10.59SDD29 pKa = 3.99LNYY32 pKa = 10.84DD33 pKa = 3.68YY34 pKa = 11.72VLDD37 pKa = 3.91EE38 pKa = 5.57DD39 pKa = 4.57GNEE42 pKa = 3.94FVIDD46 pKa = 3.65QFGDD50 pKa = 3.72CEE52 pKa = 4.28FGVFDD57 pKa = 4.57GGLVASFVLPP67 pKa = 3.98

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TB42|A0A5J6TB42_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS_SB28 OX=2591136 PE=4 SV=1
MM1 pKa = 7.56AKK3 pKa = 10.17VIYY6 pKa = 9.95RR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.92NLEE12 pKa = 3.92QEE14 pKa = 4.37LGPVGTQNFMSKK26 pKa = 9.68RR27 pKa = 11.84ARR29 pKa = 11.84KK30 pKa = 9.03AMEE33 pKa = 4.37SIRR36 pKa = 11.84VNRR39 pKa = 11.84VVRR42 pKa = 11.84VFNGEE47 pKa = 3.69GKK49 pKa = 10.08RR50 pKa = 11.84RR51 pKa = 11.84VMDD54 pKa = 3.54EE55 pKa = 4.36LIIVFF60 pKa = 4.5

Molecular weight: 7.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13672

31

980

187.3

21.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.66 ± 0.587

1.448 ± 0.204

6.159 ± 0.267

6.824 ± 0.287

3.467 ± 0.153

7.022 ± 0.267

1.865 ± 0.204

6.363 ± 0.216

6.539 ± 0.327

7.014 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.13 ± 0.196

4.849 ± 0.241

3.174 ± 0.248

3.986 ± 0.378

5.508 ± 0.252

6.532 ± 0.312

5.449 ± 0.376

6.641 ± 0.23

1.529 ± 0.114

3.833 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski