Ralstonia phage Gamede

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Cimandefvirus; Ralstonia virus Gamede

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5BA31|A0A7G5BA31_9CAUD Peptidase S74 domain-containing protein OS=Ralstonia phage Gamede OX=2759726 GN=9Ga_00035 PE=4 SV=1
MM1 pKa = 7.53LGIVQKK7 pKa = 9.44TATEE11 pKa = 3.89QLDD14 pKa = 3.56YY15 pKa = 11.26DD16 pKa = 3.64IDD18 pKa = 3.85FARR21 pKa = 11.84WMPDD25 pKa = 2.55GDD27 pKa = 4.34LLQSAGVVITPDD39 pKa = 4.17DD40 pKa = 3.68GTLASPAYY48 pKa = 9.25EE49 pKa = 3.65IDD51 pKa = 3.57GTVVKK56 pKa = 10.55VWLAGGTAGASYY68 pKa = 11.0NVDD71 pKa = 3.27VTVATAAGRR80 pKa = 11.84IKK82 pKa = 9.03EE83 pKa = 4.4TCFKK87 pKa = 10.58IRR89 pKa = 11.84VRR91 pKa = 11.84SCC93 pKa = 2.82

Molecular weight: 9.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5BA01|A0A7G5BA01_9CAUD Uncharacterized protein OS=Ralstonia phage Gamede OX=2759726 GN=9Ga_00005 PE=4 SV=1
MM1 pKa = 7.58AVIRR5 pKa = 11.84NKK7 pKa = 10.49GVVYY11 pKa = 10.37AGKK14 pKa = 10.34VGAKK18 pKa = 8.42MRR20 pKa = 11.84LRR22 pKa = 11.84SSAPRR27 pKa = 11.84KK28 pKa = 6.84TVRR31 pKa = 11.84KK32 pKa = 9.13SAYY35 pKa = 8.31KK36 pKa = 10.44CVGGPMDD43 pKa = 3.8GHH45 pKa = 5.73TLYY48 pKa = 9.86LTTPSTVPMNMAGQRR63 pKa = 11.84GRR65 pKa = 11.84YY66 pKa = 8.95VSDD69 pKa = 3.24VPMGITKK76 pKa = 9.99PILEE80 pKa = 4.32WEE82 pKa = 4.24AAA84 pKa = 3.53

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

17524

29

4639

265.5

28.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.279 ± 0.6

0.685 ± 0.211

6.152 ± 0.204

6.403 ± 0.427

3.03 ± 0.149

8.651 ± 0.402

1.735 ± 0.142

4.451 ± 0.273

4.833 ± 0.318

6.956 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.688 ± 0.139

3.452 ± 0.219

4.862 ± 0.152

4.32 ± 0.245

7.076 ± 0.322

5.649 ± 0.244

5.387 ± 0.306

6.511 ± 0.222

1.312 ± 0.177

2.568 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski