Haemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2002 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8F710|B8F710_HAEPS Putative phage associated protein OS=Haemophilus parasuis serovar 5 (strain SH0165) OX=557723 GN=HAPS_1558 PE=4 SV=1
MM1 pKa = 7.53 SKK3 pKa = 10.22 SICIITGSTLGGAEE17 pKa = 3.95 YY18 pKa = 10.87 VADD21 pKa = 4.16 HH22 pKa = 6.75 LNDD25 pKa = 3.13 VLTEE29 pKa = 3.62 QGYY32 pKa = 8.38 QVGLFNQAEE41 pKa = 4.19 LADD44 pKa = 4.13 IQHH47 pKa = 5.07 QTALLVVTSTHH58 pKa = 6.42 GAGEE62 pKa = 4.35 LPEE65 pKa = 5.07 NIQPLFQQLAEE76 pKa = 4.49 SNLDD80 pKa = 3.43 LSQMKK85 pKa = 10.16 FGVVGLGSSDD95 pKa = 3.48 YY96 pKa = 10.8 DD97 pKa = 3.85 TFCNAVNLVEE107 pKa = 4.51 QGLTAKK113 pKa = 10.54 GATQVCEE120 pKa = 4.0 SLRR123 pKa = 11.84 IDD125 pKa = 3.44 VVNNFDD131 pKa = 3.83 HH132 pKa = 7.56 DD133 pKa = 4.21 GSAEE137 pKa = 3.8 EE138 pKa = 4.28 WLPAFTSTLL147 pKa = 3.51
Molecular weight: 15.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.831
IPC2_protein 4.075
IPC_protein 3.999
Toseland 3.821
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.884
Rodwell 3.846
Grimsley 3.732
Solomon 3.961
Lehninger 3.923
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.126
Patrickios 0.947
IPC_peptide 3.961
IPC2_peptide 4.101
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|B8F7V6|B8F7V6_HAEPS Transcriptional regulator OS=Haemophilus parasuis serovar 5 (strain SH0165) OX=557723 GN=HAPS_1920 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.91 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.15 GRR39 pKa = 11.84 KK40 pKa = 8.87 SLSAA44 pKa = 3.86
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2002
0
2002
604561
35
2159
302.0
33.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.415 ± 0.062
0.991 ± 0.02
5.011 ± 0.039
6.565 ± 0.062
4.315 ± 0.051
6.829 ± 0.102
2.069 ± 0.026
6.886 ± 0.045
6.286 ± 0.054
10.317 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.372 ± 0.023
4.648 ± 0.038
3.821 ± 0.039
4.918 ± 0.048
4.51 ± 0.045
5.737 ± 0.043
5.248 ± 0.042
6.748 ± 0.05
1.133 ± 0.022
3.182 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here