Idiomarina sp. MD25a

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Idiomarina; unclassified Idiomarina

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2SX24|A0A1S2SX24_9GAMM Uncharacterized protein OS=Idiomarina sp. MD25a OX=1889913 GN=BFR57_09790 PE=4 SV=1
MM1 pKa = 7.17KK2 pKa = 9.91HH3 pKa = 5.64IAKK6 pKa = 9.97ILPLAGLALAPMAANADD23 pKa = 4.05TIFGIFAGGQYY34 pKa = 10.26WNGNADD40 pKa = 3.45GSISAFEE47 pKa = 4.15PQVQLGNYY55 pKa = 8.67DD56 pKa = 3.76DD57 pKa = 4.07QSEE60 pKa = 4.72TFSSYY65 pKa = 10.61YY66 pKa = 9.88VALEE70 pKa = 4.17HH71 pKa = 6.48PVPLIPNFKK80 pKa = 10.66ARR82 pKa = 11.84WNDD85 pKa = 3.08MTVDD89 pKa = 3.69GEE91 pKa = 4.8GINPEE96 pKa = 4.11SDD98 pKa = 3.43FSHH101 pKa = 6.97VDD103 pKa = 3.19YY104 pKa = 10.95ILYY107 pKa = 10.68YY108 pKa = 10.47EE109 pKa = 4.91IFDD112 pKa = 3.96NDD114 pKa = 3.42IVSIDD119 pKa = 3.56LGVNAKK125 pKa = 10.29RR126 pKa = 11.84FDD128 pKa = 4.04GNSSYY133 pKa = 10.73IVAGSTVTPVEE144 pKa = 4.21FSGTVPMAYY153 pKa = 10.01AATKK157 pKa = 10.02IGLPFTSWWINAEE170 pKa = 3.79ASAVSFDD177 pKa = 4.55DD178 pKa = 4.0SDD180 pKa = 3.58IRR182 pKa = 11.84DD183 pKa = 3.1ISASVEE189 pKa = 3.98YY190 pKa = 10.8QFLDD194 pKa = 3.23NAAIDD199 pKa = 3.88ASVSLGYY206 pKa = 10.19RR207 pKa = 11.84DD208 pKa = 4.09MEE210 pKa = 4.58IKK212 pKa = 10.69LDD214 pKa = 5.14DD215 pKa = 4.32IDD217 pKa = 4.28DD218 pKa = 4.24TYY220 pKa = 11.98SDD222 pKa = 3.67VRR224 pKa = 11.84FSGPYY229 pKa = 10.01LSVDD233 pKa = 3.16VHH235 pKa = 6.27FF236 pKa = 5.53

Molecular weight: 25.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2SY96|A0A1S2SY96_9GAMM RNA-binding protein Hfq OS=Idiomarina sp. MD25a OX=1889913 GN=hfq PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.38NGRR28 pKa = 11.84KK29 pKa = 9.36VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2463

0

2463

833508

37

1867

338.4

37.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.166 ± 0.045

0.875 ± 0.016

5.846 ± 0.042

6.469 ± 0.048

3.921 ± 0.029

6.789 ± 0.05

2.287 ± 0.027

5.813 ± 0.035

4.536 ± 0.044

10.294 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.441 ± 0.024

3.835 ± 0.033

4.038 ± 0.028

5.26 ± 0.047

5.415 ± 0.033

6.244 ± 0.037

5.205 ± 0.034

7.201 ± 0.039

1.384 ± 0.017

2.98 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski