Lachnoclostridium sp. An131

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium; unclassified Lachnoclostridium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3214 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y4SFH2|A0A1Y4SFH2_9FIRM Type III pantothenate kinase OS=Lachnoclostridium sp. An131 OX=1965555 GN=coaX PE=3 SV=1
MM1 pKa = 7.3KK2 pKa = 10.39RR3 pKa = 11.84KK4 pKa = 9.53LLSLTLAAVLALSLTACGGGSDD26 pKa = 3.88TADD29 pKa = 3.4TATDD33 pKa = 3.32AAEE36 pKa = 4.28TTEE39 pKa = 4.16EE40 pKa = 4.27TTEE43 pKa = 4.07EE44 pKa = 4.03TTDD47 pKa = 3.46AAEE50 pKa = 4.42STDD53 pKa = 3.5TAAGEE58 pKa = 4.16VANADD63 pKa = 3.88KK64 pKa = 10.82PLVWFNRR71 pKa = 11.84QPSNSSTGEE80 pKa = 3.83LDD82 pKa = 3.19MTALNFNEE90 pKa = 3.48NTYY93 pKa = 11.09YY94 pKa = 11.02VGFDD98 pKa = 3.41ANQGAEE104 pKa = 4.2LQGTMIRR111 pKa = 11.84DD112 pKa = 3.68YY113 pKa = 11.24IEE115 pKa = 5.38ANIDD119 pKa = 3.57TIDD122 pKa = 3.66RR123 pKa = 11.84NGDD126 pKa = 3.46GVIGYY131 pKa = 8.74VLAVGDD137 pKa = 4.11IGHH140 pKa = 6.84NDD142 pKa = 3.2SIARR146 pKa = 11.84TRR148 pKa = 11.84GVRR151 pKa = 11.84SALGTAVEE159 pKa = 4.16VDD161 pKa = 3.62GAIDD165 pKa = 3.66STPVGTNTDD174 pKa = 3.07GSSTYY179 pKa = 10.46VQDD182 pKa = 3.37GTLEE186 pKa = 4.27INGTTYY192 pKa = 9.72TVRR195 pKa = 11.84EE196 pKa = 4.12LASQEE201 pKa = 4.09MKK203 pKa = 10.84NSAGATWDD211 pKa = 3.21AATAGNAISTWSASFGDD228 pKa = 3.87QIDD231 pKa = 3.7IVASNNDD238 pKa = 3.37GMGMAMFNGWSKK250 pKa = 11.84AEE252 pKa = 3.93GVPTFGYY259 pKa = 10.15DD260 pKa = 3.27ANSDD264 pKa = 3.32AVAAIAEE271 pKa = 4.99GYY273 pKa = 10.39GGTISQHH280 pKa = 6.65ADD282 pKa = 2.86VQAYY286 pKa = 7.43LTLRR290 pKa = 11.84VLRR293 pKa = 11.84NALDD297 pKa = 3.9GVDD300 pKa = 3.46IDD302 pKa = 4.39TGIGTADD309 pKa = 3.28EE310 pKa = 4.84AGNVLTPDD318 pKa = 2.88VFTYY322 pKa = 10.9NEE324 pKa = 4.07EE325 pKa = 3.9QRR327 pKa = 11.84SYY329 pKa = 10.92YY330 pKa = 9.96ALNVAVTAEE339 pKa = 4.11NYY341 pKa = 10.16QDD343 pKa = 4.63FLDD346 pKa = 4.05STVVYY351 pKa = 10.65APVSNQLDD359 pKa = 4.15AEE361 pKa = 4.32THH363 pKa = 4.83PTKK366 pKa = 10.82NVWLNIYY373 pKa = 9.75NAADD377 pKa = 3.49NFLSATYY384 pKa = 10.31QPLLQNYY391 pKa = 9.65DD392 pKa = 3.59DD393 pKa = 4.97LLNLNVEE400 pKa = 4.63YY401 pKa = 10.44IGGDD405 pKa = 3.26GQTEE409 pKa = 4.46SNITNRR415 pKa = 11.84LGNPSQYY422 pKa = 11.16DD423 pKa = 3.19AFAINMVKK431 pKa = 9.57TDD433 pKa = 3.35NAASYY438 pKa = 10.24TSLLSQQ444 pKa = 4.08

Molecular weight: 47.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y4SE37|A0A1Y4SE37_9FIRM DNA topoisomerase (ATP-hydrolyzing) OS=Lachnoclostridium sp. An131 OX=1965555 GN=B5E77_08100 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.62VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.61GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.59LSAA44 pKa = 3.95

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3214

0

3214

1029157

25

3623

320.2

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.393 ± 0.053

1.547 ± 0.023

5.307 ± 0.042

8.327 ± 0.053

3.991 ± 0.031

7.615 ± 0.048

1.667 ± 0.02

6.4 ± 0.039

5.687 ± 0.042

9.803 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.873 ± 0.023

3.551 ± 0.026

3.525 ± 0.024

3.35 ± 0.022

5.422 ± 0.04

5.694 ± 0.033

5.124 ± 0.05

6.747 ± 0.03

1.007 ± 0.014

3.971 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski