Staphylococcus phage TEM126

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; Staphylococcus virus TEM126

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9LT51|E9LT51_9CAUD Uncharacterized protein OS=Staphylococcus phage TEM126 OX=947066 PE=4 SV=1
MM1 pKa = 7.38TPNLQLYY8 pKa = 8.19NKK10 pKa = 10.13AYY12 pKa = 7.67EE13 pKa = 4.13TLQGYY18 pKa = 7.3GFPVISHH25 pKa = 6.98KK26 pKa = 10.67EE27 pKa = 3.86MQQEE31 pKa = 3.54IPYY34 pKa = 8.78PFFVIKK40 pKa = 10.08MPEE43 pKa = 4.15SNRR46 pKa = 11.84SKK48 pKa = 10.44YY49 pKa = 9.35TFDD52 pKa = 4.18SYY54 pKa = 11.85SGDD57 pKa = 3.55TNLVIDD63 pKa = 4.3IWSVSDD69 pKa = 5.13DD70 pKa = 4.13LGHH73 pKa = 7.54HH74 pKa = 7.1DD75 pKa = 4.81GLVKK79 pKa = 10.54RR80 pKa = 11.84CIDD83 pKa = 3.9DD84 pKa = 3.54LTPSVKK90 pKa = 10.15TNDD93 pKa = 3.22YY94 pKa = 11.29DD95 pKa = 4.92FEE97 pKa = 5.84EE98 pKa = 5.91DD99 pKa = 3.81DD100 pKa = 3.91TNITQLVDD108 pKa = 3.19DD109 pKa = 4.48TTNQEE114 pKa = 4.31LLHH117 pKa = 6.13TSLTISYY124 pKa = 7.98KK125 pKa = 9.72TFF127 pKa = 3.25

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9LT74|E9LT74_9CAUD Phage anti-repressor protein OS=Staphylococcus phage TEM126 OX=947066 PE=4 SV=1
MM1 pKa = 7.81LSPFIFSAIHH11 pKa = 5.75SLGLRR16 pKa = 11.84PLKK19 pKa = 11.09ANGGTFNFSLSIATSSKK36 pKa = 10.19FPAEE40 pKa = 4.04TLPKK44 pKa = 9.54YY45 pKa = 8.26GTSISLSNSGKK56 pKa = 8.43TISSISPKK64 pKa = 10.31RR65 pKa = 3.44

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11513

63

910

217.2

24.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.663 ± 0.428

0.539 ± 0.078

5.958 ± 0.494

7.452 ± 0.63

4.36 ± 0.246

5.654 ± 0.375

1.616 ± 0.167

7.635 ± 0.404

9.068 ± 0.541

7.513 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.163

7.105 ± 0.292

2.614 ± 0.254

3.744 ± 0.213

4.247 ± 0.318

5.863 ± 0.259

6.219 ± 0.284

6.471 ± 0.436

1.129 ± 0.2

4.56 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski