Erysipelothrix phage phi1605
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K5B294|A0A2K5B294_9CAUD Major tail protein OS=Erysipelothrix phage phi1605 OX=2006938 GN=SER90K_49 PE=4 SV=1
MM1 pKa = 7.7 SILEE5 pKa = 4.28 DD6 pKa = 3.6 LQVVLEE12 pKa = 4.23 TLDD15 pKa = 3.46 VPIEE19 pKa = 3.92 IGVFSDD25 pKa = 3.7 VAPGKK30 pKa = 10.83 YY31 pKa = 8.43 MVIVPMSDD39 pKa = 3.1 SFDD42 pKa = 3.48 LHH44 pKa = 7.69 SDD46 pKa = 3.48 NLPCMDD52 pKa = 3.52 VQEE55 pKa = 4.49 ARR57 pKa = 11.84 ISIYY61 pKa = 11.02 SKK63 pKa = 11.04 GSYY66 pKa = 8.2 TALKK70 pKa = 9.15 NQVVQLLLDD79 pKa = 3.5 SSFTITARR87 pKa = 11.84 SYY89 pKa = 10.9 IGFEE93 pKa = 4.5 DD94 pKa = 3.49 DD95 pKa = 2.56 TGYY98 pKa = 11.04 YY99 pKa = 9.56 HH100 pKa = 7.27 YY101 pKa = 11.1 NVDD104 pKa = 3.47 VAKK107 pKa = 10.29 HH108 pKa = 4.82 YY109 pKa = 10.36 EE110 pKa = 3.76 IGGNN114 pKa = 3.43
Molecular weight: 12.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.953
IPC2_protein 4.177
IPC_protein 4.113
Toseland 3.91
ProMoST 4.279
Dawson 4.101
Bjellqvist 4.253
Wikipedia 4.037
Rodwell 3.948
Grimsley 3.821
Solomon 4.088
Lehninger 4.05
Nozaki 4.215
DTASelect 4.457
Thurlkill 3.961
EMBOSS 4.05
Sillero 4.24
Patrickios 0.693
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.103
Protein with the highest isoelectric point:
>tr|A0A2K5B267|A0A2K5B267_9CAUD Uncharacterized protein OS=Erysipelothrix phage phi1605 OX=2006938 GN=SER90K_19 PE=4 SV=1
MM1 pKa = 6.75 VGKK4 pKa = 10.14 RR5 pKa = 11.84 GEE7 pKa = 3.97 IHH9 pKa = 5.23 GQEE12 pKa = 3.9 YY13 pKa = 9.48 KK14 pKa = 11.32 ANIKK18 pKa = 8.25 TSCYY22 pKa = 10.04 GKK24 pKa = 7.81 TAKK27 pKa = 10.22 SVAGSALAQTKK38 pKa = 9.61 TSGKK42 pKa = 10.3
Molecular weight: 4.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.056
IPC2_protein 9.341
IPC_protein 9.268
Toseland 10.175
ProMoST 9.838
Dawson 10.306
Bjellqvist 9.926
Wikipedia 10.423
Rodwell 11.082
Grimsley 10.35
Solomon 10.35
Lehninger 10.335
Nozaki 10.175
DTASelect 9.911
Thurlkill 10.189
EMBOSS 10.555
Sillero 10.218
Patrickios 10.906
IPC_peptide 10.35
IPC2_peptide 8.492
IPC2.peptide.svr19 8.27
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
84
0
84
26662
42
1087
317.4
36.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.237 ± 0.287
0.971 ± 0.109
6.642 ± 0.18
7.655 ± 0.207
3.976 ± 0.14
6.151 ± 0.195
1.515 ± 0.081
7.674 ± 0.221
7.951 ± 0.306
8.732 ± 0.262
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.412 ± 0.105
5.513 ± 0.173
3.162 ± 0.118
3.248 ± 0.149
4.52 ± 0.19
6.339 ± 0.196
5.645 ± 0.206
6.215 ± 0.189
1.125 ± 0.112
4.317 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here