Lactobacillus phage Satyr
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K9V567|A0A2K9V567_9CAUD Glutaredoxin domain-containing protein OS=Lactobacillus phage Satyr OX=2070201 PE=4 SV=1
MM1 pKa = 7.82 IEE3 pKa = 4.01 LLLYY7 pKa = 10.42 RR8 pKa = 11.84 DD9 pKa = 3.73 DD10 pKa = 3.67 LKK12 pKa = 11.6 YY13 pKa = 11.13 YY14 pKa = 8.79 ITEE17 pKa = 4.29 EE18 pKa = 4.01 YY19 pKa = 10.01 TLQLDD24 pKa = 3.87 RR25 pKa = 11.84 DD26 pKa = 3.91 WLVDD30 pKa = 3.59 YY31 pKa = 10.64 IKK33 pKa = 11.16 NSTEE37 pKa = 3.86 YY38 pKa = 11.12 ASVDD42 pKa = 3.3 EE43 pKa = 5.38 FLEE46 pKa = 4.99 FYY48 pKa = 10.81 NSEE51 pKa = 4.01 DD52 pKa = 3.56 TEE54 pKa = 4.63 EE55 pKa = 4.64 IISTLDD61 pKa = 4.79 DD62 pKa = 3.25 MDD64 pKa = 3.95 EE65 pKa = 4.64 PYY67 pKa = 10.51 TLEE70 pKa = 3.81 KK71 pKa = 9.06 TGRR74 pKa = 11.84 YY75 pKa = 8.85 CDD77 pKa = 4.75 CDD79 pKa = 3.8 DD80 pKa = 4.9 LLL82 pKa = 6.19
Molecular weight: 9.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.835
IPC2_protein 3.643
IPC_protein 3.617
Toseland 3.414
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.554
Rodwell 3.452
Grimsley 3.325
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.935
Thurlkill 3.478
EMBOSS 3.554
Sillero 3.745
Patrickios 0.248
IPC_peptide 3.592
IPC2_peptide 3.706
IPC2.peptide.svr19 3.677
Protein with the highest isoelectric point:
>tr|A0A2K9V512|A0A2K9V512_9CAUD Uncharacterized protein OS=Lactobacillus phage Satyr OX=2070201 PE=4 SV=1
MM1 pKa = 7.47 VYY3 pKa = 10.96 GLIRR7 pKa = 11.84 FIIMVTVGTVIAITLALGVGFIVGTPVIWKK37 pKa = 8.48 WVVGVLIIVFGVIDD51 pKa = 3.59 RR52 pKa = 11.84 VVRR55 pKa = 3.86
Molecular weight: 5.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.999
IPC_protein 11.038
Toseland 10.994
ProMoST 11.696
Dawson 11.067
Bjellqvist 10.906
Wikipedia 11.389
Rodwell 11.067
Grimsley 11.125
Solomon 11.345
Lehninger 11.286
Nozaki 10.965
DTASelect 10.906
Thurlkill 10.994
EMBOSS 11.433
Sillero 11.023
Patrickios 11.023
IPC_peptide 11.345
IPC2_peptide 10.101
IPC2.peptide.svr19 8.783
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
111
0
111
22753
29
1887
205.0
23.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.865 ± 0.523
0.602 ± 0.084
7.265 ± 0.487
6.171 ± 0.341
3.89 ± 0.165
6.803 ± 0.451
1.688 ± 0.153
6.338 ± 0.203
8.329 ± 0.375
8.17 ± 0.276
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.496 ± 0.126
5.837 ± 0.221
2.549 ± 0.161
3.665 ± 0.323
3.828 ± 0.22
6.755 ± 0.319
6.311 ± 0.321
6.79 ± 0.251
1.2 ± 0.115
4.448 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here