Bacillus phage Nf (Bacteriophage Nf)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Salasmaviridae; Picovirinae; Beecentumtrevirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 27 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7SSP1|B7SSP1_BPNF DNA replication organizer OS=Bacillus phage Nf OX=10753 GN=16.7 PE=4 SV=1
MM1 pKa = 7.58SFTIKK6 pKa = 10.7CNVCGSTDD14 pKa = 3.19VTFTVGEE21 pKa = 4.11DD22 pKa = 3.38EE23 pKa = 4.68YY24 pKa = 11.26EE25 pKa = 4.3GVLSIDD31 pKa = 4.95CDD33 pKa = 3.53NCDD36 pKa = 4.13NEE38 pKa = 4.58DD39 pKa = 3.88EE40 pKa = 4.87IYY42 pKa = 10.75LFSWTGQIHH51 pKa = 5.0RR52 pKa = 11.84TT53 pKa = 3.61

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7SSP0|B7SSP0_BPNF Uncharacterized protein OS=Bacillus phage Nf OX=10753 PE=4 SV=1
MM1 pKa = 7.64LSTHH5 pKa = 6.68AVATHH10 pKa = 5.53YY11 pKa = 10.44LIKK14 pKa = 10.17PLRR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.62

Molecular weight: 2.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27

0

27

5930

19

861

219.6

24.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.784 ± 0.491

0.877 ± 0.162

6.037 ± 0.34

6.88 ± 0.544

3.997 ± 0.394

6.476 ± 0.81

1.551 ± 0.119

6.965 ± 0.375

7.656 ± 0.618

7.774 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.217

7.066 ± 0.5

2.782 ± 0.184

3.255 ± 0.399

3.963 ± 0.35

6.762 ± 0.485

6.695 ± 0.524

6.796 ± 0.493

1.18 ± 0.201

4.772 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski