Beggiatoa alba B18LD
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3440 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3CHS3|I3CHS3_9GAMM Electron transfer flavoprotein beta subunit OS=Beggiatoa alba B18LD OX=395493 GN=BegalDRAFT_2312 PE=3 SV=1
MM1 pKa = 7.73 KK2 pKa = 9.05 KK3 pKa = 8.41 TKK5 pKa = 10.12 KK6 pKa = 9.92 PSYY9 pKa = 9.12 TVLFEE14 pKa = 4.17 KK15 pKa = 11.13 LEE17 pKa = 4.19 DD18 pKa = 3.9 RR19 pKa = 11.84 LLLSANPIVALDD31 pKa = 3.75 VPEE34 pKa = 4.24 EE35 pKa = 3.9 QAIGSSVAEE44 pKa = 3.94 QLDD47 pKa = 3.73 LHH49 pKa = 6.55 LQASPAVSDD58 pKa = 3.89 SASDD62 pKa = 3.5 ALQTEE67 pKa = 4.8 KK68 pKa = 10.41 EE69 pKa = 4.56 TLTADD74 pKa = 3.52 ASAGQTASSTASIDD88 pKa = 3.43 ASTKK92 pKa = 9.91 SATGNTSTTASNSATATNTEE112 pKa = 4.45 TTTSSTTATDD122 pKa = 3.61 SSDD125 pKa = 3.44 STITTVTKK133 pKa = 10.33 GVKK136 pKa = 8.55 ITVDD140 pKa = 3.56 TDD142 pKa = 3.63 SLTPTASATSASATTSSSTDD162 pKa = 2.9 KK163 pKa = 8.88 TTHH166 pKa = 7.31 DD167 pKa = 4.66 DD168 pKa = 3.71 LSASSTDD175 pKa = 3.01 TTANDD180 pKa = 3.52 TSTQASNTSTTATTSSHH197 pKa = 5.52 ATSVDD202 pKa = 3.45 KK203 pKa = 10.96 PSTTASQSTLASTTDD218 pKa = 2.98 STTDD222 pKa = 3.03 KK223 pKa = 10.99 TSDD226 pKa = 3.34 VPQDD230 pKa = 3.46 DD231 pKa = 4.06 TTSIRR236 pKa = 11.84 VEE238 pKa = 3.71 IAFVDD243 pKa = 3.91 SRR245 pKa = 11.84 IADD248 pKa = 3.66 YY249 pKa = 8.84 QTLVDD254 pKa = 4.06 SYY256 pKa = 11.1 KK257 pKa = 10.26 AQSSEE262 pKa = 3.82 AVQMQVVVLSTEE274 pKa = 3.91 QNGISQITDD283 pKa = 2.95 VLNFYY288 pKa = 10.39 KK289 pKa = 10.85 NEE291 pKa = 4.41 GIAIDD296 pKa = 4.96 GIHH299 pKa = 6.51 ILSHH303 pKa = 6.56 AEE305 pKa = 4.07 SGSLTLGNAHH315 pKa = 7.01 FSLKK319 pKa = 10.49 DD320 pKa = 3.18 IQSNSNAAEE329 pKa = 4.06 LVKK332 pKa = 9.76 TWSDD336 pKa = 3.42 ALSSGADD343 pKa = 3.01 ILLYY347 pKa = 10.88 GCDD350 pKa = 3.47 VASGEE355 pKa = 4.24 LGVEE359 pKa = 5.02 FVQEE363 pKa = 4.04 LQKK366 pKa = 10.67 LTGADD371 pKa = 3.37 VAASTDD377 pKa = 3.35 KK378 pKa = 10.75 TGKK381 pKa = 10.25 DD382 pKa = 3.39 GLGGNWDD389 pKa = 4.7 LEE391 pKa = 4.6 TQTGQIDD398 pKa = 4.07 TQVVTGDD405 pKa = 3.67 IDD407 pKa = 3.59 TLLAGDD413 pKa = 4.65 PAPTTTIGLPSEE425 pKa = 3.83 WMINEE430 pKa = 4.04 NFTFTIDD437 pKa = 3.77 FDD439 pKa = 4.01 NTGTDD444 pKa = 2.87 MGYY447 pKa = 11.0 APYY450 pKa = 9.74 IDD452 pKa = 5.07 LKK454 pKa = 10.61 IPQGMDD460 pKa = 3.48 LLAGKK465 pKa = 9.71 YY466 pKa = 9.6 FIAEE470 pKa = 4.02 LLMVKK475 pKa = 10.04 VATWDD480 pKa = 3.4 GTQWLDD486 pKa = 3.42 SSSNPVTTHH495 pKa = 5.98 YY496 pKa = 10.9 YY497 pKa = 9.66 GDD499 pKa = 3.88 RR500 pKa = 11.84 GVSLPSFGTPQVGDD514 pKa = 3.07 QWYY517 pKa = 8.98 TIEE520 pKa = 5.11 LPFGSFAPDD529 pKa = 3.01 QPVAEE534 pKa = 4.51 FQFTAKK540 pKa = 10.52 LNPLTGAVFNTPLSISATSGFSFAQDD566 pKa = 3.14 EE567 pKa = 4.73 FNNPVADD574 pKa = 4.08 SPNLANITRR583 pKa = 11.84 EE584 pKa = 4.21 TAHH587 pKa = 6.61 SASITPKK594 pKa = 10.49 VITITKK600 pKa = 8.33 TADD603 pKa = 3.22 AAEE606 pKa = 4.83 GEE608 pKa = 4.44 AATGEE613 pKa = 4.21 NFPVKK618 pKa = 9.69 YY619 pKa = 8.93 TLTVNVANGATVTGMDD635 pKa = 4.29 IIDD638 pKa = 4.05 YY639 pKa = 10.49 LPSNLQHH646 pKa = 6.87 LNLANYY652 pKa = 9.06 HH653 pKa = 6.28 IEE655 pKa = 4.2 VAPGTVVTTAAPTTGHH671 pKa = 7.31 PNTAASGNNDD681 pKa = 3.44 YY682 pKa = 11.08 LFEE685 pKa = 4.43 NATVTGTTATNEE697 pKa = 3.91 IVITYY702 pKa = 8.98 YY703 pKa = 11.09 NIVPEE708 pKa = 3.97 NDD710 pKa = 2.95 ANKK713 pKa = 10.45 NVIISPTTGNEE724 pKa = 3.84 VTSVNEE730 pKa = 4.12 SQVSGTYY737 pKa = 9.3 TNQSGIDD744 pKa = 3.42 VTDD747 pKa = 3.41 QVVTDD752 pKa = 3.88 GTVNDD757 pKa = 4.99 ANDD760 pKa = 3.85 GTPTDD765 pKa = 5.46 DD766 pKa = 4.81 EE767 pKa = 6.6 DD768 pKa = 6.19 DD769 pKa = 5.19 DD770 pKa = 4.07 YY771 pKa = 12.08 TLIDD775 pKa = 3.96 RR776 pKa = 11.84 SIAIQKK782 pKa = 8.64 SHH784 pKa = 7.03 EE785 pKa = 4.12 IVSNITATANQALTLPGSVVKK806 pKa = 8.82 YY807 pKa = 8.36 TLEE810 pKa = 4.05 FQISDD815 pKa = 3.54 YY816 pKa = 11.27 FDD818 pKa = 4.18 FNNVVIQDD826 pKa = 4.6 LFSDD830 pKa = 4.54 GQRR833 pKa = 11.84 LYY835 pKa = 10.69 TPTGTYY841 pKa = 10.76 APTLQILGDD850 pKa = 4.12 SDD852 pKa = 4.66 SVLATTAFSGFNTSTQTATNFTLTFDD878 pKa = 3.98 PSAYY882 pKa = 6.21 PTGYY886 pKa = 10.3 EE887 pKa = 3.79 SSSYY891 pKa = 10.81 FNGVPLYY898 pKa = 10.72 DD899 pKa = 3.51 ATLLTFNVSDD909 pKa = 3.83 EE910 pKa = 4.31 LTSQGLDD917 pKa = 3.15 RR918 pKa = 11.84 ILEE921 pKa = 4.22 GNRR924 pKa = 11.84 IAAADD929 pKa = 4.32 PYY931 pKa = 11.52 ASALNPWTNGTNTPNGTRR949 pKa = 11.84 GVITYY954 pKa = 7.43 YY955 pKa = 10.75 TLVQEE960 pKa = 5.39 DD961 pKa = 4.16 FTALHH966 pKa = 5.96 TASGYY971 pKa = 6.21 TTDD974 pKa = 4.26 ASVDD978 pKa = 3.14 TGDD981 pKa = 4.04 RR982 pKa = 11.84 LFNNVAIKK990 pKa = 10.86 GNIVNSSGTVIGTEE1004 pKa = 4.56 DD1005 pKa = 4.25 DD1006 pKa = 3.79 NSADD1010 pKa = 3.56 TLTIADD1016 pKa = 3.43 IDD1018 pKa = 3.91 ITKK1021 pKa = 10.44 SLYY1024 pKa = 10.73 AINGNTAAVTNPTVLQPGDD1043 pKa = 3.55 VVTYY1047 pKa = 10.17 RR1048 pKa = 11.84 LTVEE1052 pKa = 5.15 LPTADD1057 pKa = 3.89 AEE1059 pKa = 4.21 NFYY1062 pKa = 11.07 LSDD1065 pKa = 3.99 YY1066 pKa = 11.05 LPLPTLATISTTTVEE1081 pKa = 4.84 TYY1083 pKa = 10.55 SGSPPAAGHH1092 pKa = 5.67 VSFGPSHH1099 pKa = 6.29 TLNSVIDD1106 pKa = 4.2 NYY1108 pKa = 11.29 DD1109 pKa = 3.18 ATALAPTISKK1119 pKa = 10.89 DD1120 pKa = 4.08 DD1121 pKa = 4.32 LNNSLKK1127 pKa = 10.34 IYY1129 pKa = 10.54 FGDD1132 pKa = 3.78 FDD1134 pKa = 4.86 AVANTPTAGAAVHH1147 pKa = 5.6 ATIDD1151 pKa = 3.41 ILISVQASADD1161 pKa = 3.69 PFSDD1165 pKa = 3.62 GLYY1168 pKa = 9.03 LTNQAEE1174 pKa = 4.44 SGLGNTEE1181 pKa = 4.01 GKK1183 pKa = 10.3 QNATSEE1189 pKa = 4.27 IVQVYY1194 pKa = 8.93 LVNPDD1199 pKa = 3.62 LSIKK1203 pKa = 10.36 KK1204 pKa = 10.47 GIIATDD1210 pKa = 3.21 NTSAVFSATPMPTGVTADD1228 pKa = 4.81 PISDD1232 pKa = 3.51 TTGAAFTGTVNSSNLSTNRR1251 pKa = 11.84 IDD1253 pKa = 4.38 SNLNLSQIDD1262 pKa = 3.25 AGDD1265 pKa = 3.69 TVRR1268 pKa = 11.84 YY1269 pKa = 9.71 AITIEE1274 pKa = 4.04 NTGGRR1279 pKa = 11.84 DD1280 pKa = 3.27 AYY1282 pKa = 10.62 NVQVSDD1288 pKa = 3.74 TVPTGFRR1295 pKa = 11.84 VPDD1298 pKa = 3.67 GTTLSSTNLRR1308 pKa = 11.84 VTDD1311 pKa = 3.91 GAGNAIAFTVDD1322 pKa = 3.14 SFDD1325 pKa = 3.39 ATTGNFTITLTDD1337 pKa = 3.51 GATEE1341 pKa = 4.1 GALNQGYY1348 pKa = 6.26 TTAPSGGYY1356 pKa = 9.25 INKK1359 pKa = 7.52 TTVSNGSNIAIITFDD1374 pKa = 3.91 LVAEE1378 pKa = 5.06 GSSDD1382 pKa = 3.53 ANTVVASSVEE1392 pKa = 4.26 TNTTKK1397 pKa = 10.86 LEE1399 pKa = 4.17 QYY1401 pKa = 10.2 TSVEE1405 pKa = 4.0 TGGTNFVSNGEE1416 pKa = 4.27 EE1417 pKa = 3.97 PSDD1420 pKa = 3.43 TATITIAAPNNLAKK1434 pKa = 10.58 SIITTGVTDD1443 pKa = 3.77 TNNNATGQATIGEE1456 pKa = 4.53 TVQYY1460 pKa = 10.14 QVTVKK1465 pKa = 10.36 IPEE1468 pKa = 4.39 GNTNDD1473 pKa = 3.59 FKK1475 pKa = 11.55 LVDD1478 pKa = 3.7 TLDD1481 pKa = 3.68 AGLAFVSLDD1490 pKa = 3.46 SVTSSSALTVGGSAITLASLNAGLSVTGNGNSTPQTVTFFGSSGSDD1536 pKa = 2.46 IVNSNTNNSVGEE1548 pKa = 4.57 TITLTYY1554 pKa = 8.77 TAVVLNTTGANGTDD1568 pKa = 3.82 HH1569 pKa = 7.4 NDD1571 pKa = 3.3 TVNNSVKK1578 pKa = 10.1 ATWANDD1584 pKa = 3.18 ATGITASAPNVTIRR1598 pKa = 11.84 EE1599 pKa = 4.19 PQVSITISDD1608 pKa = 3.74 GGLTQADD1615 pKa = 3.76 AGDD1618 pKa = 4.08 VITYY1622 pKa = 7.37 TVVVTANSNRR1632 pKa = 11.84 PTAHH1636 pKa = 6.99 DD1637 pKa = 3.61 VQVRR1641 pKa = 11.84 DD1642 pKa = 4.11 VIPTGLTYY1650 pKa = 10.79 VAGSATITGTTGTVVGATISEE1671 pKa = 4.52 SAGTVSASATSIAPNSTLTFTFQATVNTGLSPNSTLTTPAEE1712 pKa = 4.2 VIYY1715 pKa = 10.25 TSMAGSVSDD1724 pKa = 3.91 TSPHH1728 pKa = 5.26 ITGTTDD1734 pKa = 3.08 TEE1736 pKa = 4.24 RR1737 pKa = 11.84 TGTFGGVTQPNDD1749 pKa = 3.38 YY1750 pKa = 10.7 RR1751 pKa = 11.84 STANDD1756 pKa = 3.49 TVTITAPEE1764 pKa = 4.36 PGKK1767 pKa = 10.79 KK1768 pKa = 9.74 IIDD1771 pKa = 3.5 TSEE1774 pKa = 3.44 AHH1776 pKa = 5.76 TSVDD1780 pKa = 3.07 KK1781 pKa = 11.45 SADD1784 pKa = 3.42 GAVQLAVGEE1793 pKa = 4.19 IVRR1796 pKa = 11.84 YY1797 pKa = 9.11 EE1798 pKa = 3.72 LTINLSEE1805 pKa = 4.36 ANSKK1809 pKa = 10.05 NFQIVDD1815 pKa = 3.76 TLPAGLVYY1823 pKa = 10.88 LGDD1826 pKa = 3.73 NTTLSISNAVDD1837 pKa = 3.07 TGGSIVFSNAAIGSALNNTITFPNASVNYY1866 pKa = 8.65 NAAGNTLTFSLGDD1879 pKa = 3.54 INNQQTDD1886 pKa = 3.62 SADD1889 pKa = 3.57 VEE1891 pKa = 4.59 LVTISYY1897 pKa = 10.23 NAIVTNTTGVDD1908 pKa = 3.24 RR1909 pKa = 11.84 GNTLANSAQVYY1920 pKa = 9.71 VNSAPLGSLASVTATVVEE1938 pKa = 4.32 PSVSIDD1944 pKa = 3.26 KK1945 pKa = 11.02 AIIAPTPTNPDD1956 pKa = 2.99 AGDD1959 pKa = 3.51 TVTYY1963 pKa = 9.44 TITVTNDD1970 pKa = 2.63 NNTNGADD1977 pKa = 3.52 AFNLNLTDD1985 pKa = 3.49 VVPSHH1990 pKa = 6.67 LRR1992 pKa = 11.84 FDD1994 pKa = 3.78 SATVSTAPATTPSPNITITPVGGSFPDD2021 pKa = 3.5 NTGDD2025 pKa = 3.53 TFTITADD2032 pKa = 3.72 RR2033 pKa = 11.84 LNKK2036 pKa = 9.99 GDD2038 pKa = 3.95 SFTITVVATVLDD2050 pKa = 4.71 SITSVTNVDD2059 pKa = 2.87 NTANLTYY2066 pKa = 10.43 TSLPNSGTPNGSGDD2080 pKa = 3.46 NTTGSTAGTAGTSTGEE2096 pKa = 4.07 RR2097 pKa = 11.84 TGSGTGANDD2106 pKa = 3.14 YY2107 pKa = 7.85 TATDD2111 pKa = 3.79 TVTFTSPRR2119 pKa = 11.84 PEE2121 pKa = 3.94 GTKK2124 pKa = 9.66 TLVSTSEE2131 pKa = 3.71 AHH2133 pKa = 5.44 TSYY2136 pKa = 11.11 TNGIEE2141 pKa = 3.92 RR2142 pKa = 11.84 VAVGEE2147 pKa = 4.12 VVRR2150 pKa = 11.84 YY2151 pKa = 8.68 QLSAKK2156 pKa = 10.24 LPEE2159 pKa = 4.32 GTFNLLTLQDD2169 pKa = 4.16 YY2170 pKa = 10.61 LPNGLQFLNDD2180 pKa = 3.72 GTAKK2184 pKa = 10.17 IAFVSDD2190 pKa = 3.45 GGITLNAGNGALDD2203 pKa = 4.13 TISAVGNGALVTGNSVVTPTFTLSADD2229 pKa = 4.04 NISSQLNTDD2238 pKa = 3.11 ADD2240 pKa = 4.49 VYY2242 pKa = 11.52 ASGSDD2247 pKa = 3.3 VIFRR2251 pKa = 11.84 LGTVVNADD2259 pKa = 3.39 HH2260 pKa = 7.7 DD2261 pKa = 5.48 SNDD2264 pKa = 2.96 EE2265 pKa = 3.94 WVVIEE2270 pKa = 4.66 FNAIVVNEE2278 pKa = 4.14 VANQAGTTAYY2288 pKa = 10.46 NGFGIILGDD2297 pKa = 3.63 NLTGYY2302 pKa = 9.92 VGPATDD2308 pKa = 2.87 ISNYY2312 pKa = 9.51 VGVSVVEE2319 pKa = 4.54 PIVGVTKK2326 pKa = 9.67 TASPTAGDD2334 pKa = 3.79 ADD2336 pKa = 3.85 DD2337 pKa = 4.02 TVTYY2341 pKa = 9.49 TITVTNSATGNNAAAAFDD2359 pKa = 3.48 IHH2361 pKa = 8.39 VLDD2364 pKa = 4.79 NLNALDD2370 pKa = 4.63 AGAHH2374 pKa = 5.97 SIVSITPNVGATGANLTNASTGNIVDD2400 pKa = 3.71 YY2401 pKa = 10.36 TIDD2404 pKa = 3.49 RR2405 pKa = 11.84 LDD2407 pKa = 3.8 PSEE2410 pKa = 4.76 SVTFTVVTTLTGNVTPAQLVNNEE2433 pKa = 3.96 AKK2435 pKa = 9.03 VTYY2438 pKa = 9.74 TSLPNTGTTSNPTGSNTSGASGDD2461 pKa = 3.65 ADD2463 pKa = 3.71 GEE2465 pKa = 4.31 RR2466 pKa = 11.84 DD2467 pKa = 3.69 GSDD2470 pKa = 3.02 GTGGLNDD2477 pKa = 3.8 YY2478 pKa = 9.57 TDD2480 pKa = 3.73 SDD2482 pKa = 3.9 TTEE2485 pKa = 3.31 ITIYY2489 pKa = 10.7 SNNISGFVYY2498 pKa = 10.48 TDD2500 pKa = 3.18 INNDD2504 pKa = 3.97 GIFDD2508 pKa = 4.39 ANGADD2513 pKa = 3.76 NIAGNDD2519 pKa = 3.69 DD2520 pKa = 5.46 DD2521 pKa = 6.16 EE2522 pKa = 6.41 IPLNNVTITLTGTDD2536 pKa = 3.45 HH2537 pKa = 7.15 LGNAVSEE2544 pKa = 4.51 TTTTNTLGYY2553 pKa = 10.67 YY2554 pKa = 9.55 EE2555 pKa = 4.27 FTGLRR2560 pKa = 11.84 PSNATGYY2567 pKa = 10.13 IVTQTQPTGYY2577 pKa = 11.04 ADD2579 pKa = 3.65 GQEE2582 pKa = 4.04 IAGTGGTTSVVTDD2595 pKa = 3.48 NQIKK2599 pKa = 10.37 VIIPEE2604 pKa = 4.35 GADD2607 pKa = 3.07 TSSTHH2612 pKa = 6.43 NDD2614 pKa = 2.97 FFEE2617 pKa = 5.56 IPAQLGDD2624 pKa = 3.95 RR2625 pKa = 11.84 VWRR2628 pKa = 11.84 DD2629 pKa = 3.16 VNANGIQDD2637 pKa = 3.62 AGEE2640 pKa = 3.78 QGIAGLTVNLLDD2652 pKa = 4.83 NSGNLIKK2659 pKa = 10.89 SAVTDD2664 pKa = 3.57 TNGNYY2669 pKa = 10.02 HH2670 pKa = 7.32 FYY2672 pKa = 11.39 NLIAGDD2678 pKa = 3.79 YY2679 pKa = 9.16 RR2680 pKa = 11.84 IEE2682 pKa = 4.09 FTGVGSGTLADD2693 pKa = 4.02 SAFTTADD2700 pKa = 3.15 EE2701 pKa = 4.41 ATATEE2706 pKa = 4.36 ATDD2709 pKa = 3.81 SDD2711 pKa = 4.16 VTGNTGTGRR2720 pKa = 11.84 TNLITLTAGEE2730 pKa = 4.5 TNNDD2734 pKa = 2.41 IDD2736 pKa = 5.51 AGIRR2740 pKa = 11.84 WTGSIGDD2747 pKa = 4.06 YY2748 pKa = 10.89 VWIDD2752 pKa = 3.46 TNNNNLQDD2760 pKa = 4.29 AGDD2763 pKa = 4.14 ALLSNITVEE2772 pKa = 5.28 LYY2774 pKa = 10.81 DD2775 pKa = 3.61 STGTTLLATTTTDD2788 pKa = 2.74 SSGNYY2793 pKa = 9.72 LFEE2796 pKa = 4.72 NLAAGSYY2803 pKa = 6.88 QVKK2806 pKa = 8.05 VTPPSSMGFVTADD2819 pKa = 2.93 TGGDD2823 pKa = 3.53 DD2824 pKa = 4.83 TIDD2827 pKa = 3.64 SDD2829 pKa = 4.02 VDD2831 pKa = 3.44 STGIVNVTLTTANLNRR2847 pKa = 11.84 TDD2849 pKa = 3.31 IDD2851 pKa = 3.59 AGLKK2855 pKa = 10.52 FNGTGSIGDD2864 pKa = 3.71 FVFIDD2869 pKa = 3.72 ANANGIQDD2877 pKa = 3.62 AGEE2880 pKa = 3.9 IGINNATVQLFDD2892 pKa = 3.84 GLGNLLSTTTTNTTGGYY2909 pKa = 10.64 LFTNLVAGNYY2919 pKa = 6.61 TIKK2922 pKa = 10.73 VSGVSASLTPTGKK2935 pKa = 10.17 DD2936 pKa = 3.04 LGGNDD2941 pKa = 3.96 ATDD2944 pKa = 3.37 SDD2946 pKa = 3.87 IDD2948 pKa = 3.79 ATGEE2952 pKa = 4.06 TSTITLTTGQNKK2964 pKa = 9.99 LDD2966 pKa = 3.44 VDD2968 pKa = 3.71 AGYY2971 pKa = 11.06 VYY2973 pKa = 10.38 TGTGSIGDD2981 pKa = 4.07 QVWLDD2986 pKa = 3.4 SDD2988 pKa = 4.08 ADD2990 pKa = 4.51 GIQDD2994 pKa = 3.45 AGEE2997 pKa = 4.2 SGVAGVKK3004 pKa = 10.22 VEE3006 pKa = 5.18 LYY3008 pKa = 10.63 DD3009 pKa = 3.74 STGTTLLASIDD3020 pKa = 3.79 TDD3022 pKa = 3.55 SSGNYY3027 pKa = 10.21 LFDD3030 pKa = 4.69 NLDD3033 pKa = 3.14 TSLDD3037 pKa = 3.71 YY3038 pKa = 10.24 TVKK3041 pKa = 9.22 VTAPTGRR3048 pKa = 11.84 AFTTANVGGNEE3059 pKa = 4.3 SLDD3062 pKa = 3.61 SDD3064 pKa = 4.15 ADD3066 pKa = 3.7 SSGVISAIDD3075 pKa = 3.55 LSVTANRR3082 pKa = 11.84 TDD3084 pKa = 2.98 IDD3086 pKa = 4.25 AGLTLNGTGSIGNFVFIDD3104 pKa = 3.59 ANANGIQDD3112 pKa = 3.59 AGEE3115 pKa = 3.97 IGITGATVTLLDD3127 pKa = 3.91 NSNNPLATTTTDD3139 pKa = 2.96 SNGAYY3144 pKa = 9.96 VFNGLAAGDD3153 pKa = 3.99 YY3154 pKa = 9.49 KK3155 pKa = 10.85 IQVSGVSASLTPTGKK3170 pKa = 10.17 DD3171 pKa = 3.04 LGGNDD3176 pKa = 4.02 ATDD3179 pKa = 3.38 SDD3181 pKa = 5.34 LNTTGISDD3189 pKa = 5.04 TITLTAGQSNQTVDD3203 pKa = 2.69 AGYY3206 pKa = 10.64 VYY3208 pKa = 10.27 TGTGSIGDD3216 pKa = 4.91 HH3217 pKa = 6.15 IWLDD3221 pKa = 3.41 SDD3223 pKa = 4.45 ADD3225 pKa = 4.51 GIQDD3229 pKa = 3.45 AGEE3232 pKa = 4.2 SGVAGVKK3239 pKa = 10.22 VEE3241 pKa = 5.18 LYY3243 pKa = 10.63 DD3244 pKa = 3.74 STGTTLLASIDD3255 pKa = 3.79 TDD3257 pKa = 3.55 SSGNYY3262 pKa = 10.21 LFDD3265 pKa = 4.69 NLDD3268 pKa = 3.14 TSLDD3272 pKa = 3.71 YY3273 pKa = 9.98 TVKK3276 pKa = 10.78 VIAPTGRR3283 pKa = 11.84 AFTTANVGGNEE3294 pKa = 4.3 SLDD3297 pKa = 3.61 SDD3299 pKa = 4.15 ADD3301 pKa = 3.7 SSGVISAIDD3310 pKa = 3.55 LSVTANRR3317 pKa = 11.84 TDD3319 pKa = 2.98 IDD3321 pKa = 4.25 AGLTLNGTGSIGNFVFIDD3339 pKa = 3.57 ANANGVQDD3347 pKa = 3.84 AGEE3350 pKa = 4.07 IGITGATVTLLDD3362 pKa = 3.91 NSNNPLATTTTDD3374 pKa = 2.96 SNGAYY3379 pKa = 9.96 VFNGLAAGDD3388 pKa = 3.99 YY3389 pKa = 9.49 KK3390 pKa = 10.85 IQVSGVSASLTPTTQNAGADD3410 pKa = 3.8 DD3411 pKa = 4.34 TVDD3414 pKa = 3.39 SDD3416 pKa = 4.59 IDD3418 pKa = 3.68 ATSITATITLTAGQSNQTVDD3438 pKa = 2.69 AGYY3441 pKa = 10.64 VYY3443 pKa = 10.27 TGTGSIGDD3451 pKa = 4.59 RR3452 pKa = 11.84 IWLDD3456 pKa = 3.04 SDD3458 pKa = 3.95 ADD3460 pKa = 4.51 GIQDD3464 pKa = 3.45 AGEE3467 pKa = 4.2 SGVAGVKK3474 pKa = 10.22 VEE3476 pKa = 5.18 LYY3478 pKa = 10.63 DD3479 pKa = 3.74 STGTTLLASIDD3490 pKa = 3.5 TDD3492 pKa = 3.84 TNGNYY3497 pKa = 10.45 LFDD3500 pKa = 4.52 NLDD3503 pKa = 3.14 TSLDD3507 pKa = 3.71 YY3508 pKa = 10.24 TVKK3511 pKa = 9.22 VTAPTGRR3518 pKa = 11.84 AFTTANVGGNEE3529 pKa = 4.3 SLDD3532 pKa = 3.61 SDD3534 pKa = 4.15 ADD3536 pKa = 3.7 SSGVISAIDD3545 pKa = 3.42 LSVTNNRR3552 pKa = 11.84 TDD3554 pKa = 2.9 VDD3556 pKa = 3.96 AGLTLNGTGSIGNFVFIDD3574 pKa = 3.59 ANANGIQDD3582 pKa = 3.59 AGEE3585 pKa = 3.97 IGITGATVTLLDD3597 pKa = 3.91 NSNNPLATTTTDD3609 pKa = 2.96 SNGAYY3614 pKa = 9.96 VFNGLAAGDD3623 pKa = 3.99 YY3624 pKa = 9.49 KK3625 pKa = 10.85 IQVSGVSASLTPTTQNAGADD3645 pKa = 3.8 DD3646 pKa = 4.27 TLDD3649 pKa = 3.54 SDD3651 pKa = 4.77 IDD3653 pKa = 3.77 ATSITATITLTTGQSNQTVDD3673 pKa = 2.69 AGYY3676 pKa = 10.69 VYY3678 pKa = 10.35 TGTSSIGDD3686 pKa = 4.11 RR3687 pKa = 11.84 VWLDD3691 pKa = 3.22 YY3692 pKa = 11.54 DD3693 pKa = 3.89 GDD3695 pKa = 4.31 GVQDD3699 pKa = 3.4 ATEE3702 pKa = 4.26 TVGVAGVTVSLYY3714 pKa = 11.04 DD3715 pKa = 3.51 SAGTTLLASIDD3726 pKa = 3.5 TDD3728 pKa = 3.83 VNGNYY3733 pKa = 10.47 LFDD3736 pKa = 3.98 NLAAGDD3742 pKa = 4.13 YY3743 pKa = 9.75 IIKK3746 pKa = 8.26 VTKK3749 pKa = 9.06 PSSMNISSINQGGNDD3764 pKa = 4.39 ALDD3767 pKa = 3.73 SDD3769 pKa = 4.24 IDD3771 pKa = 3.9 TTTGEE3776 pKa = 4.24 TAVITLAANTDD3787 pKa = 3.32 ITHH3790 pKa = 6.19 VDD3792 pKa = 3.09 AGLQYY3797 pKa = 10.34 PEE3799 pKa = 5.17 SISGTVFHH3807 pKa = 7.44 DD3808 pKa = 3.81 LDD3810 pKa = 4.05 NDD3812 pKa = 4.04 GVIDD3816 pKa = 4.31 AGEE3819 pKa = 3.99 TGIAGVTMTLKK3830 pKa = 10.88 DD3831 pKa = 3.23 SGGTVIATTTTDD3843 pKa = 2.83 SNGQYY3848 pKa = 10.27 FFAVNAGNTYY3858 pKa = 9.97 IIEE3861 pKa = 4.19 EE3862 pKa = 4.49 TQPSAYY3868 pKa = 10.51 LDD3870 pKa = 4.07 GQDD3873 pKa = 3.26 TAGVNGGNTSVNDD3886 pKa = 4.43 TISSITINSGDD3897 pKa = 3.53 NATGYY3902 pKa = 11.03 NFGEE3906 pKa = 4.47 VLTSSVSGYY3915 pKa = 8.83 TYY3917 pKa = 11.07 EE3918 pKa = 6.32 DD3919 pKa = 4.31 DD3920 pKa = 5.9 DD3921 pKa = 5.03 NDD3923 pKa = 4.05 GVKK3926 pKa = 10.57 DD3927 pKa = 3.68 AGEE3930 pKa = 3.97 SAIAGVTITLTGTDD3944 pKa = 3.73 DD3945 pKa = 3.73 LGNAVSLSTTTDD3957 pKa = 2.79 SNGYY3961 pKa = 9.65 YY3962 pKa = 10.16 EE3963 pKa = 4.54 FTDD3966 pKa = 4.35 LRR3968 pKa = 11.84 PSDD3971 pKa = 3.48 ATGYY3975 pKa = 8.68 TLTQTHH3981 pKa = 6.03 PTAYY3985 pKa = 9.65 TDD3987 pKa = 3.75 GTDD3990 pKa = 3.25 TAGTPFGGASSEE4002 pKa = 4.35 TATVRR4007 pKa = 11.84 DD4008 pKa = 4.13 DD4009 pKa = 4.24 KK4010 pKa = 11.0 ITGIVVINASGTTGTDD4026 pKa = 3.35 YY4027 pKa = 11.61 NFGEE4031 pKa = 4.36 LKK4033 pKa = 9.32 KK4034 pKa = 8.85 TSLSGYY4040 pKa = 9.38 VYY4042 pKa = 11.15 NDD4044 pKa = 3.12 IDD4046 pKa = 3.92 NDD4048 pKa = 4.11 GVKK4051 pKa = 10.49 DD4052 pKa = 3.66 ATEE4055 pKa = 4.01 SGIAGTSVRR4064 pKa = 11.84 LTGTDD4069 pKa = 3.45 DD4070 pKa = 3.82 LGNTVDD4076 pKa = 3.6 VTVATDD4082 pKa = 3.26 SNGYY4086 pKa = 9.66 YY4087 pKa = 10.27 EE4088 pKa = 4.54 FTDD4091 pKa = 4.35 LRR4093 pKa = 11.84 PSDD4096 pKa = 3.48 ATGYY4100 pKa = 8.56 TLTEE4104 pKa = 4.0 TQPNGFDD4111 pKa = 4.94 DD4112 pKa = 5.91 GIDD4115 pKa = 3.71 TIGSQGGTSNNDD4127 pKa = 3.01 SFSDD4131 pKa = 3.75 LVLVAGTVGTDD4142 pKa = 3.1 NNFGEE4147 pKa = 4.52 IFNPLLEE4154 pKa = 4.2 KK4155 pKa = 10.41 TIVATSEE4162 pKa = 3.77 TGTTGNNPTIGEE4174 pKa = 4.09 IVRR4177 pKa = 11.84 YY4178 pKa = 9.52 RR4179 pKa = 11.84 LTAEE4183 pKa = 4.17 VYY4185 pKa = 9.88 EE4186 pKa = 4.4 GTMTNLQIRR4195 pKa = 11.84 EE4196 pKa = 4.25 SLPAGLQFLNDD4207 pKa = 3.53 GTATVAFVSTNTSNISTNDD4226 pKa = 3.0 TTLNSLTGLKK4236 pKa = 10.21 VAGDD4240 pKa = 3.92 EE4241 pKa = 4.46 TSVGTITPSAFLPDD4255 pKa = 4.19 ANISTSVSTNSDD4267 pKa = 3.44 VYY4269 pKa = 11.53 ASGTDD4274 pKa = 3.38 VYY4276 pKa = 10.98 FKK4278 pKa = 10.93 LGNLTNSNLTANKK4291 pKa = 8.91 EE4292 pKa = 4.0 FVVIEE4297 pKa = 4.26 FNALVLNEE4305 pKa = 4.41 SANQAGTNLEE4315 pKa = 4.09 NTFTLLYY4322 pKa = 10.18 DD4323 pKa = 3.66 QNGDD4327 pKa = 3.81 TNPEE4331 pKa = 3.91 DD4332 pKa = 4.13 SKK4334 pKa = 10.19 TSDD4337 pKa = 3.11 TVTVTIVEE4345 pKa = 4.26 PEE4347 pKa = 3.61 ISNLTKK4353 pKa = 10.35 TISSSTATPPDD4364 pKa = 3.48 EE4365 pKa = 5.53 GDD4367 pKa = 3.37 TLVYY4371 pKa = 10.42 QITYY4375 pKa = 9.97 SNTGTAPAYY4384 pKa = 10.05 DD4385 pKa = 3.65 VLLTDD4390 pKa = 5.51 FLDD4393 pKa = 4.66 DD4394 pKa = 4.17 ADD4396 pKa = 4.44 KK4397 pKa = 11.15 VAPFDD4402 pKa = 4.03 GSLEE4406 pKa = 4.08 LVDD4409 pKa = 5.36 ARR4411 pKa = 11.84 IASSSPALNAGDD4423 pKa = 3.45 ITITEE4428 pKa = 4.2 NFTDD4432 pKa = 3.76 NSAEE4436 pKa = 3.86 ILINTLEE4443 pKa = 4.28 IGQSVTVEE4451 pKa = 3.73 IEE4453 pKa = 4.31 VKK4455 pKa = 10.56 VKK4457 pKa = 10.92 AGTAPATDD4465 pKa = 4.59 LDD4467 pKa = 3.87 NTADD4471 pKa = 3.13 ISYY4474 pKa = 10.94 NSLPNGGTGTPSNPTGSDD4492 pKa = 3.15 TPTTSGTSTGGRR4504 pKa = 11.84 DD4505 pKa = 3.63 GSDD4508 pKa = 3.06 GSSGLNDD4515 pKa = 3.72 YY4516 pKa = 10.87 VDD4518 pKa = 4.04 SDD4520 pKa = 3.96 NAPTEE4525 pKa = 4.21 TTGNANSLGDD4535 pKa = 3.86 YY4536 pKa = 10.44 VWFDD4540 pKa = 3.77 FNGDD4544 pKa = 3.44 GVQDD4548 pKa = 3.95 ANEE4551 pKa = 3.95 KK4552 pKa = 10.38 GIPNVTVNLYY4562 pKa = 8.98 WAGADD4567 pKa = 4.02 DD4568 pKa = 4.63 VLDD4571 pKa = 4.24 AADD4574 pKa = 4.81 LVTPYY4579 pKa = 9.43 KK4580 pKa = 8.81 TTTTDD4585 pKa = 2.87 ANGYY4589 pKa = 7.88 YY4590 pKa = 9.94 QFTDD4594 pKa = 4.19 LPASTFKK4601 pKa = 11.16 VVLDD4605 pKa = 3.81 STNLTSLTPTSDD4617 pKa = 3.31 YY4618 pKa = 11.74 DD4619 pKa = 4.49 GIATANEE4626 pKa = 3.89 ATVTLTNTTDD4636 pKa = 3.25 EE4637 pKa = 4.37 TDD4639 pKa = 2.82 VDD4641 pKa = 4.48 FGYY4644 pKa = 10.86 VGSNSIGDD4652 pKa = 4.02 TVYY4655 pKa = 11.38 LDD4657 pKa = 4.27 LDD4659 pKa = 3.99 ADD4661 pKa = 4.07 GVEE4664 pKa = 4.54 DD4665 pKa = 3.6 MGEE4668 pKa = 3.71 IGLPNAEE4675 pKa = 4.42 VTLTWTFNSATITTTTTTDD4694 pKa = 2.75 QDD4696 pKa = 3.47 GKK4698 pKa = 10.94 YY4699 pKa = 10.41 LFAGLPAGTDD4709 pKa = 3.53 YY4710 pKa = 11.2 QVTVTPSSVSGANLTTVGSYY4730 pKa = 11.54 SNISLTNNTDD4740 pKa = 2.9 HH4741 pKa = 6.63 LTADD4745 pKa = 4.13 FGFVGTGSIGDD4756 pKa = 4.1 YY4757 pKa = 10.16 VWNDD4761 pKa = 3.12 ANGDD4765 pKa = 3.7 ADD4767 pKa = 3.72 QDD4769 pKa = 3.84 LGEE4772 pKa = 4.82 AGIAGVSLDD4781 pKa = 4.04 IYY4783 pKa = 10.8 LDD4785 pKa = 3.76 RR4786 pKa = 11.84 NGDD4789 pKa = 3.8 GLLDD4793 pKa = 4.33 AGDD4796 pKa = 3.83 VKK4798 pKa = 10.42 ITTTTTNPSGYY4809 pKa = 8.57 YY4810 pKa = 9.21 TFNHH4814 pKa = 6.16 LPEE4817 pKa = 4.42 AKK4819 pKa = 10.37 YY4820 pKa = 10.52 IVDD4823 pKa = 3.65 VTDD4826 pKa = 3.49 TSNLLDD4832 pKa = 3.85 GFILTGGTDD4841 pKa = 3.71 PQAVTLPAGGNIVSVDD4857 pKa = 3.85 FGYY4860 pKa = 9.89 QQQGAVIGDD4869 pKa = 4.3 YY4870 pKa = 10.25 VWHH4873 pKa = 7.22 DD4874 pKa = 3.52 ADD4876 pKa = 4.28 GDD4878 pKa = 4.36 SVQDD4882 pKa = 3.55 VTEE4885 pKa = 3.98 TGIAGVQVDD4894 pKa = 5.44 LYY4896 pKa = 11.67 VDD4898 pKa = 3.7 TNSNGVLDD4906 pKa = 4.69 IGDD4909 pKa = 3.98 TLADD4913 pKa = 3.67 TRR4915 pKa = 11.84 TTDD4918 pKa = 3.23 ANGYY4922 pKa = 7.88 YY4923 pKa = 9.8 QFTDD4927 pKa = 3.58 LTVGRR4932 pKa = 11.84 YY4933 pKa = 8.82 LVDD4936 pKa = 3.03 IVEE4939 pKa = 4.51 ATLPTNYY4946 pKa = 9.97 QNTTNNEE4953 pKa = 3.81 PFVANITTPTQIISNADD4970 pKa = 3.04 FGYY4973 pKa = 10.42 RR4974 pKa = 11.84 EE4975 pKa = 3.73 QAGIGDD4981 pKa = 4.52 YY4982 pKa = 11.02 VWLDD4986 pKa = 3.44 ANGDD4990 pKa = 3.81 GVQDD4994 pKa = 3.55 VGEE4997 pKa = 4.46 TGLSNVSLALYY5008 pKa = 9.95 RR5009 pKa = 11.84 DD5010 pKa = 3.77 NNGDD5014 pKa = 3.84 GIINGTDD5021 pKa = 3.39 SVIKK5025 pKa = 9.79 TVLTDD5030 pKa = 3.55 ANGAYY5035 pKa = 10.46 LFKK5038 pKa = 11.12 NLDD5041 pKa = 3.48 AGSYY5045 pKa = 7.16 VVKK5048 pKa = 9.81 VTDD5051 pKa = 3.44 SFGVLAGLSQSYY5063 pKa = 9.25 GANTYY5068 pKa = 11.07 AKK5070 pKa = 8.89 TLIAGEE5076 pKa = 4.11 YY5077 pKa = 10.72 DD5078 pKa = 3.05 KK5079 pKa = 11.58 DD5080 pKa = 3.65 ADD5082 pKa = 5.01 FGFTDD5087 pKa = 4.53 PATGSIGDD5095 pKa = 4.44 YY5096 pKa = 9.95 IWNDD5100 pKa = 2.98 ADD5102 pKa = 3.84 GDD5104 pKa = 4.53 GIQEE5108 pKa = 4.17 TGEE5111 pKa = 4.02 TGIDD5115 pKa = 3.58 GVTLEE5120 pKa = 4.82 LRR5122 pKa = 11.84 TAGTDD5127 pKa = 3.54 GLFGTGDD5134 pKa = 3.74 DD5135 pKa = 3.93 VVVDD5139 pKa = 3.94 TTTTASGGAYY5149 pKa = 10.02 HH5150 pKa = 6.03 FTGLTKK5156 pKa = 10.26 GAYY5159 pKa = 8.57 QVVVTDD5165 pKa = 4.23 TGNLLNNFALTKK5177 pKa = 10.65 DD5178 pKa = 3.54 PDD5180 pKa = 4.19 SIADD5184 pKa = 3.58 EE5185 pKa = 4.22 KK5186 pKa = 11.21 HH5187 pKa = 6.17 AVTLSVGQMVNTVDD5201 pKa = 4.81 FGYY5204 pKa = 9.19 QQRR5207 pKa = 11.84 DD5208 pKa = 3.23 GRR5210 pKa = 11.84 IGDD5213 pKa = 4.26 LVWNDD5218 pKa = 3.3 TNADD5222 pKa = 4.04 GIVDD5226 pKa = 3.84 GGEE5229 pKa = 4.01 SGVVGVSVTLYY5240 pKa = 10.78 RR5241 pKa = 11.84 DD5242 pKa = 3.54 TNNNGVLDD5250 pKa = 3.95 TGDD5253 pKa = 2.97 VSLAVTTTDD5262 pKa = 3.81 ANGLYY5267 pKa = 10.33 EE5268 pKa = 4.43 FTNLAAGKK5276 pKa = 9.94 YY5277 pKa = 9.75 LVDD5280 pKa = 3.59 VDD5282 pKa = 4.15 TTGTLAGYY5290 pKa = 7.41 TLTSTSTKK5298 pKa = 9.64 GAEE5301 pKa = 4.06 PMPVTLSAGQNFIEE5315 pKa = 5.11 ADD5317 pKa = 3.67 FGYY5320 pKa = 10.57 HH5321 pKa = 7.4 DD5322 pKa = 4.97 PQTGNIGDD5330 pKa = 4.21 RR5331 pKa = 11.84 VWNDD5335 pKa = 2.73 TDD5337 pKa = 3.92 GDD5339 pKa = 4.44 GVQDD5343 pKa = 3.46 TGEE5346 pKa = 4.27 TGLSGVSVTLRR5357 pKa = 11.84 NAGTDD5362 pKa = 4.17 GIFGTGDD5369 pKa = 3.23 DD5370 pKa = 4.04 TTATTTTDD5378 pKa = 2.8 TSGNYY5383 pKa = 10.23 SFTGLDD5389 pKa = 2.96 AGKK5392 pKa = 10.44 YY5393 pKa = 8.81 RR5394 pKa = 11.84 VEE5396 pKa = 3.99 ITNPTGDD5403 pKa = 4.13 LPNYY5407 pKa = 9.23 AQTGDD5412 pKa = 3.56 PDD5414 pKa = 3.39 ATKK5417 pKa = 10.65 DD5418 pKa = 3.56 HH5419 pKa = 5.32 QHH5421 pKa = 6.91 EE5422 pKa = 4.43 ITLSAGQTVTTADD5435 pKa = 4.08 FGYY5438 pKa = 10.43 QLQNSSIGDD5447 pKa = 4.18 TVWSDD5452 pKa = 3.04 TDD5454 pKa = 4.37 GNGLLNGGEE5463 pKa = 4.23 TGISGVTVTLLRR5475 pKa = 11.84 DD5476 pKa = 3.24 NDD5478 pKa = 3.96 GNGTYY5483 pKa = 7.35 EE5484 pKa = 4.27 TTVATQVTDD5493 pKa = 3.69 ANGNYY5498 pKa = 10.31 SFTQLPAGKK5507 pKa = 9.94 YY5508 pKa = 9.16 RR5509 pKa = 11.84 VDD5511 pKa = 3.27 INTTGVLASYY5521 pKa = 10.17 VQTADD5526 pKa = 3.98 PDD5528 pKa = 3.63 ATKK5531 pKa = 11.02 NNSHH5535 pKa = 6.5 TLTLANSSNIDD5546 pKa = 3.55 TVDD5549 pKa = 4.1 FGYY5552 pKa = 10.22 QQLAQIGDD5560 pKa = 4.44 TVWSDD5565 pKa = 3.29 LDD5567 pKa = 3.64 SDD5569 pKa = 4.56 GVQDD5573 pKa = 3.48 TGEE5576 pKa = 4.03 AGIAGVTVALYY5587 pKa = 10.65 RR5588 pKa = 11.84 DD5589 pKa = 4.01 VNNSGTYY5596 pKa = 10.02 DD5597 pKa = 3.43 AGDD5600 pKa = 3.7 TLVTTQTTDD5609 pKa = 2.73 ANGNYY5614 pKa = 9.82 HH5615 pKa = 6.17 FLKK5618 pKa = 10.41 LAAGAYY5624 pKa = 8.85 IVTVTDD5630 pKa = 3.4 TANVLGLYY5638 pKa = 10.27 VPTTSEE5644 pKa = 3.86 TKK5646 pKa = 10.52 AITLSAGEE5654 pKa = 4.43 TNDD5657 pKa = 3.65 TADD5660 pKa = 4.54 FGYY5663 pKa = 10.97 ADD5665 pKa = 4.93 PKK5667 pKa = 9.76 TGSIGDD5673 pKa = 4.34 TIWNDD5678 pKa = 3.06 ANGNGVQDD5686 pKa = 3.6 TGEE5689 pKa = 4.16 VGLSGVTVSLNSAGTDD5705 pKa = 4.22 GIFGTADD5712 pKa = 3.26 DD5713 pKa = 4.51 VVVDD5717 pKa = 4.15 TTSTDD5722 pKa = 3.08 STGTYY5727 pKa = 9.95 HH5728 pKa = 7.28 FNNLPAGKK5736 pKa = 8.74 YY5737 pKa = 7.17 QVVTTPPTGFTQTADD5752 pKa = 3.43 PDD5754 pKa = 3.97 TTKK5757 pKa = 11.33 DD5758 pKa = 3.02 HH5759 pKa = 6.04 TTLVTLTAGQMVTTADD5775 pKa = 3.3 FGYY5778 pKa = 9.58 QQSDD5782 pKa = 3.01 AHH5784 pKa = 8.06 IGDD5787 pKa = 4.83 LVWHH5791 pKa = 7.06 DD5792 pKa = 4.16 ADD5794 pKa = 4.52 GDD5796 pKa = 4.34 GIKK5799 pKa = 10.67 DD5800 pKa = 3.43 AGEE5803 pKa = 3.95 TGIDD5807 pKa = 3.55 GVTVALYY5814 pKa = 10.65 RR5815 pKa = 11.84 DD5816 pKa = 3.41 VDD5818 pKa = 3.88 NNGVLDD5824 pKa = 3.91 IGTDD5828 pKa = 3.58 TLLEE5832 pKa = 4.21 TQTTSGGGIYY5842 pKa = 10.62 AFDD5845 pKa = 3.89 NLIADD5850 pKa = 4.19 NYY5852 pKa = 10.04 LVAVTDD5858 pKa = 3.98 TANVLYY5864 pKa = 10.25 NYY5866 pKa = 9.83 QAINGGQAVATPHH5879 pKa = 6.31 AVSLSAGEE5887 pKa = 4.13 QLDD5890 pKa = 4.22 TVDD5893 pKa = 5.28 FAYY5896 pKa = 10.38 QRR5898 pKa = 11.84 YY5899 pKa = 9.42 ASIGDD5904 pKa = 4.04 YY5905 pKa = 10.95 LWLDD5909 pKa = 4.21 DD5910 pKa = 4.18 GDD5912 pKa = 4.54 GVQEE5916 pKa = 3.91 ADD5918 pKa = 3.27 EE5919 pKa = 4.77 FGVVGNYY5926 pKa = 9.62 GLSGVTVNLYY5936 pKa = 10.84 SDD5938 pKa = 4.76 DD5939 pKa = 4.53 DD5940 pKa = 4.54 SSGTLTFGDD5949 pKa = 3.95 ALIATVTTDD5958 pKa = 3.22 VNGKK5962 pKa = 10.01 YY5963 pKa = 10.57 DD5964 pKa = 3.92 FTKK5967 pKa = 10.76 LPAGTYY5973 pKa = 9.0 IVSVTDD5979 pKa = 3.37 TNNALAGLSASTPQNKK5995 pKa = 7.95 TVTVTSGQDD6004 pKa = 3.38 YY6005 pKa = 11.34 NDD6007 pKa = 3.73 ADD6009 pKa = 3.91 YY6010 pKa = 9.34 PYY6012 pKa = 9.62 TDD6014 pKa = 4.5 PQTASIGDD6022 pKa = 4.0 YY6023 pKa = 10.97 VWDD6026 pKa = 4.56 DD6027 pKa = 3.47 KK6028 pKa = 11.7 NGNGIAEE6035 pKa = 4.2 SGEE6038 pKa = 4.19 TGFEE6042 pKa = 3.96 GVTVNLYY6049 pKa = 10.48 RR6050 pKa = 11.84 DD6051 pKa = 3.49 NNANGVYY6058 pKa = 10.39 DD6059 pKa = 4.99 AGDD6062 pKa = 3.5 TLAATTTTDD6071 pKa = 3.5 PLGHH6075 pKa = 6.25 YY6076 pKa = 10.48 SFTKK6080 pKa = 10.32 LPKK6083 pKa = 9.81 GDD6085 pKa = 4.0 YY6086 pKa = 10.51 LVDD6089 pKa = 3.5 VATGIPTGYY6098 pKa = 10.39 VLTGGTDD6105 pKa = 3.18 PHH6107 pKa = 7.42 ALNLSPAEE6115 pKa = 4.29 TYY6117 pKa = 11.19 NDD6119 pKa = 3.77 ADD6121 pKa = 3.9 FGYY6124 pKa = 10.34 QRR6126 pKa = 11.84 QTASIGDD6133 pKa = 4.44 LVWQDD6138 pKa = 3.38 QNSNGVLDD6146 pKa = 4.96 GIEE6149 pKa = 3.88 TGIAGVTLNLYY6160 pKa = 10.39 RR6161 pKa = 11.84 DD6162 pKa = 3.89 TNNNGTLDD6170 pKa = 3.64 TGDD6173 pKa = 4.06 SLVASTTTDD6182 pKa = 3.02 STGHH6186 pKa = 5.57 YY6187 pKa = 10.06 SFNNLPTGQYY6197 pKa = 10.13 FVDD6200 pKa = 3.81 VTDD6203 pKa = 3.58 TGGKK6207 pKa = 9.69 LGGYY6211 pKa = 8.21 TSTTHH6216 pKa = 6.9 NDD6218 pKa = 3.39 PLLVSLAEE6226 pKa = 4.1 NQTYY6230 pKa = 10.13 LAADD6234 pKa = 4.39 FGYY6237 pKa = 10.67 LSNPVYY6243 pKa = 10.45 QGAVGDD6249 pKa = 4.5 QLWLDD6254 pKa = 3.67 NDD6256 pKa = 4.14 GDD6258 pKa = 4.22 GQLDD6262 pKa = 3.37 TGEE6265 pKa = 4.44 PVLANIDD6272 pKa = 3.53 VTLRR6276 pKa = 11.84 EE6277 pKa = 4.3 AGSDD6281 pKa = 3.9 GVFGTADD6288 pKa = 3.3 DD6289 pKa = 4.64 VIRR6292 pKa = 11.84 TTTTNSAGQYY6302 pKa = 10.06 QFTGLPTGYY6311 pKa = 10.57 YY6312 pKa = 8.83 RR6313 pKa = 11.84 VEE6315 pKa = 4.35 VNTSQLPAGVSTTTPNSSNPFLLSNVQSKK6344 pKa = 10.69 QDD6346 pKa = 2.84 VDD6348 pKa = 3.95 FGYY6351 pKa = 10.58 RR6352 pKa = 11.84 GTGSLGDD6359 pKa = 4.15 YY6360 pKa = 10.27 VWLDD6364 pKa = 3.33 TDD6366 pKa = 4.12 KK6367 pKa = 11.71 DD6368 pKa = 4.37 GIQDD6372 pKa = 3.52 TSEE6375 pKa = 3.78 TGLAGVAVQMTWAGLDD6391 pKa = 3.53 GNLATTADD6399 pKa = 3.55 NVTYY6403 pKa = 9.32 NTVTDD6408 pKa = 3.58 ATGHH6412 pKa = 5.61 YY6413 pKa = 9.31 KK6414 pKa = 10.36 VNHH6417 pKa = 6.42 LPAGQYY6423 pKa = 9.12 QVSLTSGLPTGTTITVGAQSMGSSSATVTLPAGTNKK6459 pKa = 10.18 LDD6461 pKa = 3.29 VDD6463 pKa = 4.39 FGVATTTDD6471 pKa = 3.21 KK6472 pKa = 11.57 GSIGDD6477 pKa = 4.28 TVWLDD6482 pKa = 3.22 NDD6484 pKa = 3.63 GDD6486 pKa = 4.56 GIVDD6490 pKa = 3.65 TDD6492 pKa = 3.93 EE6493 pKa = 5.42 LPLSGMTVTLTGAGTDD6509 pKa = 4.54 GILGTSDD6516 pKa = 3.38 DD6517 pKa = 4.0 THH6519 pKa = 5.79 VTTTTDD6525 pKa = 3.01 SNGKK6529 pKa = 9.21 YY6530 pKa = 10.42 LFDD6533 pKa = 3.77 NLSPNTYY6540 pKa = 8.81 QVSVTTPSGLTLTTPNSLNPVTLTKK6565 pKa = 10.72 DD6566 pKa = 3.41 QVIDD6570 pKa = 3.85 TVDD6573 pKa = 4.04 FGYY6576 pKa = 10.6 KK6577 pKa = 8.74 PQNTASLGDD6586 pKa = 3.94 YY6587 pKa = 10.61 VWIDD6591 pKa = 3.6 SNNDD6595 pKa = 3.01 GLQTAGEE6602 pKa = 4.15 TGIAGVTMQAIWAGVDD6618 pKa = 3.36 GNLNTTADD6626 pKa = 3.75 NLIFTTVTDD6635 pKa = 3.82 SNGLYY6640 pKa = 10.53 HH6641 pKa = 7.14 FADD6644 pKa = 4.64 LPAGVYY6650 pKa = 9.63 QISVSGVSSTLAPFAGGQSVGTGTTTLTLSTGQMRR6685 pKa = 11.84 TDD6687 pKa = 2.59 VDD6689 pKa = 4.24 FGYY6692 pKa = 10.62 RR6693 pKa = 11.84 PTLGSIGDD6701 pKa = 4.29 TVWNDD6706 pKa = 2.9 TDD6708 pKa = 3.91 QDD6710 pKa = 3.87 GTQDD6714 pKa = 3.56 PDD6716 pKa = 3.23 EE6717 pKa = 4.49 VGIAGVTVTLTSAGTDD6733 pKa = 4.28 GILGTADD6740 pKa = 3.95 DD6741 pKa = 4.35 TTQTTTTDD6749 pKa = 2.72 SNGQYY6754 pKa = 10.44 RR6755 pKa = 11.84 FNDD6758 pKa = 4.0 LPTGNYY6764 pKa = 7.22 TVSITPPTGSTLTTPPVVNIPLAPQQIVDD6793 pKa = 3.58 TADD6796 pKa = 3.21 FGLYY6800 pKa = 10.12 NPNQTGSIGDD6810 pKa = 4.22 TVWFDD6815 pKa = 3.47 ADD6817 pKa = 3.17 QDD6819 pKa = 4.13 GAQDD6823 pKa = 3.36 TGEE6826 pKa = 4.11 TGVAGVIVTLTGAGSDD6842 pKa = 4.09 GVFGTADD6849 pKa = 4.65 DD6850 pKa = 3.68 ITRR6853 pKa = 11.84 TLTTDD6858 pKa = 2.61 SNGQYY6863 pKa = 10.14 RR6864 pKa = 11.84 FSGLEE6869 pKa = 3.58 AGNYY6873 pKa = 6.35 TVSVTPPTGNSLTTPPTVSINLTPQQQFDD6902 pKa = 3.43 AADD6905 pKa = 3.87 FGLYY6909 pKa = 10.51 NSTTPPPAPTGRR6921 pKa = 11.84 IGDD6924 pKa = 4.3 TVWNDD6929 pKa = 2.92 TDD6931 pKa = 4.04 GDD6933 pKa = 4.42 GVQDD6937 pKa = 3.65 PTEE6940 pKa = 4.18 TGVAGVTVKK6949 pKa = 9.53 LTNAGADD6956 pKa = 4.3 DD6957 pKa = 4.19 IFGTPDD6963 pKa = 3.32 DD6964 pKa = 4.31 TVLTTTTDD6972 pKa = 3.44 SNGHH6976 pKa = 4.59 YY6977 pKa = 10.3 QFVNLDD6983 pKa = 3.22 GGLYY6987 pKa = 9.36 QVSIVPPTGTTITTPSRR7004 pKa = 11.84 LNSFTLADD7012 pKa = 3.96 GQQIDD7017 pKa = 4.07 TVDD7020 pKa = 3.03 IGLIQPTGTATIGDD7034 pKa = 4.55 TVWSDD7039 pKa = 3.05 TDD7041 pKa = 3.58 GDD7043 pKa = 4.45 GVQDD7047 pKa = 3.45 TGEE7050 pKa = 4.15 TGVAGIVVTLQGAGKK7065 pKa = 10.24 DD7066 pKa = 4.08 GVFGTADD7073 pKa = 3.45 DD7074 pKa = 4.32 TLTNTTTDD7082 pKa = 3.41 SNGHH7086 pKa = 4.76 YY7087 pKa = 10.33 QFTHH7091 pKa = 5.88 VDD7093 pKa = 3.05 AGSYY7097 pKa = 8.51 QVSITPPNGTALTTPNSLNPFSITTGQNIDD7127 pKa = 3.74 TIDD7130 pKa = 3.53 FGLYY7134 pKa = 10.09 KK7135 pKa = 9.57 PTGTSSVGDD7144 pKa = 3.96 TVWNDD7149 pKa = 2.92 TDD7151 pKa = 4.04 GDD7153 pKa = 4.42 GVQDD7157 pKa = 3.65 PTEE7160 pKa = 4.19 TGVAGIVVTLRR7171 pKa = 11.84 GAGNDD7176 pKa = 3.99 GVLGTADD7183 pKa = 4.48 DD7184 pKa = 4.68 LVMSTTTNSAGQYY7197 pKa = 10.08 LFSNLTAGSYY7207 pKa = 9.34 QVTITPPTGTSITTPNSSNPFNLANNQQIDD7237 pKa = 4.13 TIDD7240 pKa = 3.52 FGLNKK7245 pKa = 8.97 PTGTGKK7251 pKa = 10.21 IGDD7254 pKa = 4.44 TVWHH7258 pKa = 6.9 DD7259 pKa = 3.31 VDD7261 pKa = 4.18 SDD7263 pKa = 4.08 GVQDD7267 pKa = 3.91 PDD7269 pKa = 3.32 EE7270 pKa = 4.57 TGVGGVTVILTGAGKK7285 pKa = 10.3 DD7286 pKa = 3.8 GVFGTTDD7293 pKa = 3.35 DD7294 pKa = 3.94 TTATTITDD7302 pKa = 3.23 AKK7304 pKa = 10.03 GQYY7307 pKa = 10.7 SFDD7310 pKa = 3.71 KK7311 pKa = 10.92 LDD7313 pKa = 3.7 AGLYY7317 pKa = 9.87 KK7318 pKa = 9.83 VTIVPPQDD7326 pKa = 3.53 MQVTTPNSYY7335 pKa = 11.33 NPVTLANNQTIDD7347 pKa = 3.77 TIDD7350 pKa = 3.63 FGLTKK7355 pKa = 9.39 PTGNSSIGDD7364 pKa = 3.98 TVWNDD7369 pKa = 2.69 KK7370 pKa = 11.31 DD7371 pKa = 3.6 KK7372 pKa = 11.81 DD7373 pKa = 3.94 GVQDD7377 pKa = 3.53 SDD7379 pKa = 3.65 EE7380 pKa = 4.52 TPVSGVVVTLTGAGKK7395 pKa = 10.37 DD7396 pKa = 3.98 GVFGTADD7403 pKa = 3.77 DD7404 pKa = 4.18 TTLTTTTDD7412 pKa = 3.15 SNGKK7416 pKa = 9.24 YY7417 pKa = 10.58 LFDD7420 pKa = 4.25 NLDD7423 pKa = 3.29 AGIYY7427 pKa = 9.36 KK7428 pKa = 10.13 VSIDD7432 pKa = 3.55 VSSDD7436 pKa = 3.28 KK7437 pKa = 11.08 EE7438 pKa = 3.91 ITTPNSFF7445 pKa = 3.45
Molecular weight: 770.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.363
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.579
Rodwell 3.427
Grimsley 3.261
Solomon 3.617
Lehninger 3.579
Nozaki 3.719
DTASelect 4.05
Thurlkill 3.414
EMBOSS 3.592
Sillero 3.732
Patrickios 1.024
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.684
Protein with the highest isoelectric point:
>tr|I3CEK6|I3CEK6_9GAMM Sec-independent protein translocase protein TatB OS=Beggiatoa alba B18LD OX=395493 GN=tatB PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLVRR12 pKa = 11.84 KK13 pKa = 9.31 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.45 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MQTRR25 pKa = 11.84 GGRR28 pKa = 11.84 LVLKK32 pKa = 10.24 SRR34 pKa = 11.84 RR35 pKa = 11.84 EE36 pKa = 3.93 KK37 pKa = 10.72 GRR39 pKa = 11.84 HH40 pKa = 5.02 RR41 pKa = 11.84 LSAA44 pKa = 3.8
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3440
0
3440
1223585
29
7445
355.7
39.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.409 ± 0.045
1.01 ± 0.018
5.004 ± 0.033
5.994 ± 0.047
4.087 ± 0.035
6.17 ± 0.051
2.374 ± 0.028
6.824 ± 0.037
4.871 ± 0.042
11.293 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.024
4.412 ± 0.044
4.21 ± 0.032
5.435 ± 0.047
4.757 ± 0.042
5.671 ± 0.044
6.321 ± 0.077
6.433 ± 0.041
1.246 ± 0.018
3.294 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here