Drosophila persimilis (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Schizophora; Acalyptratae;

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4H252|B4H252_DROPE GL17850 OS=Drosophila persimilis OX=7234 GN=Dper\GL17850 PE=4 SV=1
MM1 pKa = 7.76ADD3 pKa = 3.35VVLDD7 pKa = 4.77LYY9 pKa = 11.54AEE11 pKa = 4.68DD12 pKa = 4.77LDD14 pKa = 5.09KK15 pKa = 11.64DD16 pKa = 3.96FAGQSQDD23 pKa = 3.04EE24 pKa = 4.43FGGDD28 pKa = 3.89GVDD31 pKa = 4.44LYY33 pKa = 11.52DD34 pKa = 6.06DD35 pKa = 3.55IGGPTEE41 pKa = 3.97AAASGGGGGGTPSADD56 pKa = 3.07GAAGPGSGDD65 pKa = 3.63SASSGGGGPNGVYY78 pKa = 10.09HH79 pKa = 6.15QGGGSLTPTMNRR91 pKa = 11.84RR92 pKa = 11.84YY93 pKa = 9.7QLYY96 pKa = 9.61VGNLTWVDD104 pKa = 3.86NGPKK108 pKa = 9.57TSPCFEE114 pKa = 4.97GYY116 pKa = 8.85WSQRR120 pKa = 11.84LTGGEE125 pKa = 4.19VLL127 pKa = 4.66

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4G5A2|B4G5A2_DROPE GL24328 (Fragment) OS=Drosophila persimilis OX=7234 GN=Dper\GL24328 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84TGHH5 pKa = 6.8LALRR9 pKa = 11.84LLAVSLCIWMTNAATSTTATTSSGTSTTVAATTTTSAAATTTTTAATTAATTSAATTVASTGTVHH74 pKa = 7.14RR75 pKa = 11.84KK76 pKa = 9.32KK77 pKa = 10.47IRR79 pKa = 11.84ISNLKK84 pKa = 8.34WSSKK88 pKa = 9.69RR89 pKa = 11.84RR90 pKa = 11.84IKK92 pKa = 9.26VHH94 pKa = 6.75RR95 pKa = 11.84GTTNRR100 pKa = 11.84STRR103 pKa = 11.84SRR105 pKa = 11.84SVLGRR110 pKa = 11.84RR111 pKa = 11.84ILRR114 pKa = 11.84SRR116 pKa = 11.84NGSRR120 pKa = 11.84ARR122 pKa = 11.84VLVLNQRR129 pKa = 11.84RR130 pKa = 11.84AQGG133 pKa = 3.17

Molecular weight: 14.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16749

5

16754

7194521

23

8120

429.4

47.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.676 ± 0.024

1.937 ± 0.013

5.169 ± 0.016

6.289 ± 0.022

3.481 ± 0.015

6.152 ± 0.024

2.739 ± 0.013

4.77 ± 0.017

5.357 ± 0.022

9.169 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.424 ± 0.009

4.452 ± 0.012

5.501 ± 0.026

5.232 ± 0.027

5.682 ± 0.017

8.151 ± 0.025

5.645 ± 0.016

5.832 ± 0.015

1.044 ± 0.007

2.97 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski