Gemycircularvirus BZ1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas2; Human associated gemykibivirus 2

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3IQD8|A0A0E3IQD8_9VIRU Replication-associated protein OS=Gemycircularvirus BZ1 OX=1588761 PE=3 SV=1
MM1 pKa = 7.54TFHH4 pKa = 7.04CNARR8 pKa = 11.84YY9 pKa = 8.77FLLTYY14 pKa = 7.75SQCGEE19 pKa = 4.04LDD21 pKa = 3.09PWKK24 pKa = 10.99VSDD27 pKa = 4.43HH28 pKa = 6.28LGEE31 pKa = 4.32LRR33 pKa = 11.84GEE35 pKa = 4.24CIVARR40 pKa = 11.84EE41 pKa = 4.0AHH43 pKa = 6.56ADD45 pKa = 3.59GGTHH49 pKa = 6.22LHH51 pKa = 6.48AFVDD55 pKa = 4.82FGRR58 pKa = 11.84KK59 pKa = 7.98FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84TSIFDD69 pKa = 3.44VDD71 pKa = 3.42NHH73 pKa = 6.44HH74 pKa = 7.22PNVSATHH81 pKa = 4.54STPRR85 pKa = 11.84EE86 pKa = 4.05GFDD89 pKa = 3.51YY90 pKa = 10.91ACKK93 pKa = 10.75DD94 pKa = 3.18GDD96 pKa = 3.76IVAGGLEE103 pKa = 4.24RR104 pKa = 11.84PEE106 pKa = 3.72QSDD109 pKa = 3.49RR110 pKa = 11.84VVRR113 pKa = 11.84STGWHH118 pKa = 7.04DD119 pKa = 3.17IVAATSRR126 pKa = 11.84EE127 pKa = 4.12EE128 pKa = 4.12FFEE131 pKa = 4.86LLMAHH136 pKa = 7.25DD137 pKa = 4.86PRR139 pKa = 11.84TLCTCFAQLSKK150 pKa = 11.03YY151 pKa = 10.65ADD153 pKa = 2.74WRR155 pKa = 11.84YY156 pKa = 10.31RR157 pKa = 11.84EE158 pKa = 4.44DD159 pKa = 3.71PEE161 pKa = 4.8PYY163 pKa = 9.65RR164 pKa = 11.84SPDD167 pKa = 3.25SVVIDD172 pKa = 4.02ASAFPEE178 pKa = 4.39LEE180 pKa = 4.17EE181 pKa = 4.21WPHH184 pKa = 6.52RR185 pKa = 11.84AIQPAHH191 pKa = 5.09VRR193 pKa = 11.84RR194 pKa = 11.84GRR196 pKa = 11.84RR197 pKa = 11.84GQSLVLYY204 pKa = 9.68GPSRR208 pKa = 11.84MGKK211 pKa = 6.4TLWARR216 pKa = 11.84SLGSHH221 pKa = 7.06AYY223 pKa = 10.06FGGLFSLQEE232 pKa = 3.78EE233 pKa = 4.65TDD235 pKa = 3.63NVDD238 pKa = 3.18YY239 pKa = 11.36AIFDD243 pKa = 4.31DD244 pKa = 4.79LNGGIPFFPQYY255 pKa = 10.6KK256 pKa = 8.37WWLGHH261 pKa = 5.76QGQFYY266 pKa = 9.1ATDD269 pKa = 3.39KK270 pKa = 11.28YY271 pKa = 10.76KK272 pKa = 10.98GKK274 pKa = 10.69KK275 pKa = 8.08LVHH278 pKa = 6.61WGRR281 pKa = 11.84PAIWISNKK289 pKa = 9.95DD290 pKa = 3.24PRR292 pKa = 11.84EE293 pKa = 3.97EE294 pKa = 4.49HH295 pKa = 6.25GADD298 pKa = 3.86YY299 pKa = 11.16DD300 pKa = 3.89WLNANCLFIYY310 pKa = 10.42VDD312 pKa = 4.24TPIAWEE318 pKa = 3.97IEE320 pKa = 4.06

Molecular weight: 36.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3IQD8|A0A0E3IQD8_9VIRU Replication-associated protein OS=Gemycircularvirus BZ1 OX=1588761 PE=3 SV=1
MM1 pKa = 7.94AYY3 pKa = 9.67GSRR6 pKa = 11.84YY7 pKa = 8.28RR8 pKa = 11.84GYY10 pKa = 9.85RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84MFSRR19 pKa = 11.84SRR21 pKa = 11.84ARR23 pKa = 11.84RR24 pKa = 11.84GNGRR28 pKa = 11.84RR29 pKa = 11.84YY30 pKa = 9.83SRR32 pKa = 11.84APRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84VYY39 pKa = 10.09RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84TRR45 pKa = 11.84RR46 pKa = 11.84SVLNVSTRR54 pKa = 11.84KK55 pKa = 10.17LKK57 pKa = 9.82DD58 pKa = 3.02TMPPVVITEE67 pKa = 4.19GGFAGTPGVPYY78 pKa = 10.88RR79 pKa = 11.84MTGQLEE85 pKa = 4.29YY86 pKa = 10.56TFAFCPSARR95 pKa = 11.84DD96 pKa = 3.57SHH98 pKa = 6.09RR99 pKa = 11.84TTATDD104 pKa = 3.81FPVGQRR110 pKa = 11.84GMYY113 pKa = 9.23QRR115 pKa = 11.84SLTDD119 pKa = 3.09VYY121 pKa = 11.47ARR123 pKa = 11.84GYY125 pKa = 10.47KK126 pKa = 10.12EE127 pKa = 3.93IVSLASNSGANWLWRR142 pKa = 11.84RR143 pKa = 11.84LVVSLKK149 pKa = 10.56NPIWKK154 pKa = 9.36LFPSLTVQRR163 pKa = 11.84EE164 pKa = 3.72IPLDD168 pKa = 3.46SDD170 pKa = 3.53RR171 pKa = 11.84ASGQVRR177 pKa = 11.84TMYY180 pKa = 10.71NIGPTPEE187 pKa = 5.06GEE189 pKa = 4.19TEE191 pKa = 4.07LAQDD195 pKa = 3.9VYY197 pKa = 11.26RR198 pKa = 11.84VIFEE202 pKa = 4.26GTLDD206 pKa = 4.31LDD208 pKa = 3.36WHH210 pKa = 6.17NVFNAKK216 pKa = 9.0PDD218 pKa = 3.32KK219 pKa = 10.7RR220 pKa = 11.84FVRR223 pKa = 11.84VRR225 pKa = 11.84SDD227 pKa = 2.42RR228 pKa = 11.84TMRR231 pKa = 11.84LRR233 pKa = 11.84TTNDD237 pKa = 2.6EE238 pKa = 4.41AYY240 pKa = 10.84LNNYY244 pKa = 7.28QFWDD248 pKa = 3.97GVNANMHH255 pKa = 5.69YY256 pKa = 10.34NEE258 pKa = 5.09KK259 pKa = 10.64EE260 pKa = 3.96SGSDD264 pKa = 2.77IKK266 pKa = 10.43MDD268 pKa = 4.63PDD270 pKa = 3.84LEE272 pKa = 4.72SNEE275 pKa = 3.86NTLSKK280 pKa = 10.51FSAEE284 pKa = 4.21GLSGAGDD291 pKa = 3.54LWVFDD296 pKa = 4.68FFSCGSKK303 pKa = 10.51DD304 pKa = 3.36AADD307 pKa = 3.75EE308 pKa = 5.46LRR310 pKa = 11.84FQPNGTFFWHH320 pKa = 6.15EE321 pKa = 3.88RR322 pKa = 3.51

Molecular weight: 37.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

642

320

322

321.0

37.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.854 ± 0.689

1.402 ± 0.565

7.009 ± 0.577

5.763 ± 0.574

5.452 ± 0.125

7.632 ± 0.129

3.115 ± 1.354

3.115 ± 0.681

3.427 ± 0.008

6.854 ± 0.24

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.869 ± 0.669

3.894 ± 1.002

4.984 ± 0.236

2.648 ± 0.118

10.748 ± 2.109

6.854 ± 0.658

5.452 ± 0.774

5.607 ± 0.437

2.804 ± 0.455

4.517 ± 0.102
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski