SIV-wrc Pbt-05GM-X02

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Simian immunodeficiency virus; Simian immunodeficiency virus - wrc

Average proteome isoelectric point is 7.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3CKG7|B3CKG7_SIV Gag polyprotein OS=SIV-wrc Pbt-05GM-X02 OX=498715 GN=gag PE=4 SV=1
MM1 pKa = 7.44GGVFSKK7 pKa = 11.08DD8 pKa = 3.27SVEE11 pKa = 3.98QRR13 pKa = 11.84MLRR16 pKa = 11.84YY17 pKa = 9.2RR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LLRR23 pKa = 11.84VGRR26 pKa = 11.84KK27 pKa = 6.22EE28 pKa = 3.18RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 7.87TRR35 pKa = 11.84LAAEE39 pKa = 4.38RR40 pKa = 11.84QAEE43 pKa = 4.04AAASGEE49 pKa = 4.19LLPDD53 pKa = 3.73SAQTLIRR60 pKa = 11.84AEE62 pKa = 4.07EE63 pKa = 4.34DD64 pKa = 3.18SSKK67 pKa = 10.99EE68 pKa = 4.09DD69 pKa = 4.09SSDD72 pKa = 3.27DD73 pKa = 3.71DD74 pKa = 5.85AIGFPVRR81 pKa = 11.84PQVPLRR87 pKa = 11.84QATFKK92 pKa = 10.91ILVDD96 pKa = 4.33LSHH99 pKa = 7.11LFKK102 pKa = 11.05EE103 pKa = 4.43KK104 pKa = 10.86GGLEE108 pKa = 4.37DD109 pKa = 4.53IFWSPRR115 pKa = 11.84RR116 pKa = 11.84EE117 pKa = 4.05EE118 pKa = 4.05IVNLYY123 pKa = 10.24AYY125 pKa = 9.46HH126 pKa = 6.55EE127 pKa = 4.22WGIVEE132 pKa = 4.67GWQAYY137 pKa = 8.08TDD139 pKa = 4.35GPGVRR144 pKa = 11.84YY145 pKa = 8.76PKK147 pKa = 10.1TFGWCWKK154 pKa = 9.27LVPVHH159 pKa = 6.8FSEE162 pKa = 5.14EE163 pKa = 4.47SPNSDD168 pKa = 3.49QGFEE172 pKa = 4.33KK173 pKa = 10.9NLLLHH178 pKa = 6.32PAVAEE183 pKa = 4.82SEE185 pKa = 4.9DD186 pKa = 3.39PWKK189 pKa = 10.73EE190 pKa = 3.65YY191 pKa = 10.81LVWKK195 pKa = 9.8FDD197 pKa = 3.57PRR199 pKa = 11.84LACDD203 pKa = 4.13FVAGRR208 pKa = 11.84LADD211 pKa = 3.87GQIATGIEE219 pKa = 3.84TLYY222 pKa = 11.08EE223 pKa = 3.83MKK225 pKa = 10.49KK226 pKa = 10.46RR227 pKa = 11.84NNKK230 pKa = 9.0

Molecular weight: 26.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3CKH0|B3CKH0_SIV Vpr protein OS=SIV-wrc Pbt-05GM-X02 OX=498715 GN=vpr PE=4 SV=1
MM1 pKa = 7.05EE2 pKa = 5.38KK3 pKa = 10.41EE4 pKa = 4.07WIAVPLIKK12 pKa = 9.63ISKK15 pKa = 8.84GRR17 pKa = 11.84FRR19 pKa = 11.84RR20 pKa = 11.84WSYY23 pKa = 10.54WIAEE27 pKa = 4.84LIQEE31 pKa = 4.16KK32 pKa = 8.26QARR35 pKa = 11.84FLFGSWHH42 pKa = 4.24VHH44 pKa = 5.16AEE46 pKa = 3.9CFTRR50 pKa = 11.84HH51 pKa = 5.46IVRR54 pKa = 11.84IPLQGHH60 pKa = 5.66WEE62 pKa = 4.0MQIRR66 pKa = 11.84CYY68 pKa = 10.27WNLAPEE74 pKa = 4.48IGWIDD79 pKa = 3.33TLGFNIRR86 pKa = 11.84MVWEE90 pKa = 4.36NKK92 pKa = 8.25WEE94 pKa = 4.04TFITVKK100 pKa = 10.62LADD103 pKa = 3.83VLIHH107 pKa = 5.78SQHH110 pKa = 5.97FEE112 pKa = 3.93CWTQSAVKK120 pKa = 8.67NTIRR124 pKa = 11.84QGYY127 pKa = 7.36LHH129 pKa = 7.37PIDD132 pKa = 3.46SCKK135 pKa = 10.17YY136 pKa = 8.0HH137 pKa = 6.09QGHH140 pKa = 6.93LGVLTLQALCLQALQHH156 pKa = 5.33EE157 pKa = 4.73QLRR160 pKa = 11.84KK161 pKa = 9.66RR162 pKa = 11.84KK163 pKa = 9.41APNRR167 pKa = 11.84RR168 pKa = 11.84TPGNTRR174 pKa = 11.84GSSRR178 pKa = 11.84YY179 pKa = 9.49ARR181 pKa = 11.84AVCHH185 pKa = 6.38GKK187 pKa = 7.75TAFPPGSPCKK197 pKa = 10.57SISLLLATEE206 pKa = 4.27KK207 pKa = 11.01LL208 pKa = 3.79

Molecular weight: 24.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3209

23

1012

356.6

40.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.703 ± 0.422

2.586 ± 0.649

3.615 ± 0.321

7.822 ± 0.466

2.898 ± 0.395

7.074 ± 0.409

2.4 ± 0.298

6.793 ± 0.855

7.323 ± 0.657

8.632 ± 0.421

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.994 ± 0.224

4.207 ± 0.588

4.612 ± 0.745

6.139 ± 0.497

5.765 ± 0.815

5.64 ± 0.477

5.765 ± 0.552

5.36 ± 0.532

2.773 ± 0.384

2.898 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski