Amino acid dipepetide frequency for Mycobacterium phage Hope4ever

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.313AlaAla: 13.313 ± 1.439
0.691AlaCys: 0.691 ± 0.19
7.222AlaAsp: 7.222 ± 0.75
6.719AlaGlu: 6.719 ± 0.857
3.768AlaPhe: 3.768 ± 0.615
8.101AlaGly: 8.101 ± 0.878
1.319AlaHis: 1.319 ± 0.35
4.207AlaIle: 4.207 ± 0.533
4.898AlaLys: 4.898 ± 0.604
9.231AlaLeu: 9.231 ± 0.814
2.324AlaMet: 2.324 ± 0.424
2.135AlaAsn: 2.135 ± 0.339
4.396AlaPro: 4.396 ± 0.797
2.952AlaGln: 2.952 ± 0.46
5.966AlaArg: 5.966 ± 0.545
5.149AlaSer: 5.149 ± 0.566
5.966AlaThr: 5.966 ± 0.629
8.352AlaVal: 8.352 ± 0.807
1.821AlaTrp: 1.821 ± 0.376
2.7AlaTyr: 2.7 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.691CysAla: 0.691 ± 0.257
0.0CysCys: 0.0 ± 0.0
0.565CysAsp: 0.565 ± 0.171
0.754CysGlu: 0.754 ± 0.168
0.126CysPhe: 0.126 ± 0.095
0.565CysGly: 0.565 ± 0.208
0.126CysHis: 0.126 ± 0.095
0.251CysIle: 0.251 ± 0.12
0.251CysLys: 0.251 ± 0.13
0.377CysLeu: 0.377 ± 0.194
0.188CysMet: 0.188 ± 0.115
0.188CysAsn: 0.188 ± 0.098
0.251CysPro: 0.251 ± 0.129
0.188CysGln: 0.188 ± 0.095
0.377CysArg: 0.377 ± 0.145
0.502CysSer: 0.502 ± 0.194
0.314CysThr: 0.314 ± 0.14
0.314CysVal: 0.314 ± 0.141
0.251CysTrp: 0.251 ± 0.134
0.126CysTyr: 0.126 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
6.468AspAla: 6.468 ± 0.606
0.377AspCys: 0.377 ± 0.132
4.584AspAsp: 4.584 ± 0.547
3.58AspGlu: 3.58 ± 0.577
2.324AspPhe: 2.324 ± 0.403
6.217AspGly: 6.217 ± 0.655
1.13AspHis: 1.13 ± 0.29
3.014AspIle: 3.014 ± 0.4
2.449AspLys: 2.449 ± 0.409
6.971AspLeu: 6.971 ± 0.773
1.319AspMet: 1.319 ± 0.273
2.01AspAsn: 2.01 ± 0.361
4.773AspPro: 4.773 ± 0.497
1.633AspGln: 1.633 ± 0.338
3.203AspArg: 3.203 ± 0.419
3.266AspSer: 3.266 ± 0.524
3.831AspThr: 3.831 ± 0.388
4.459AspVal: 4.459 ± 0.572
1.758AspTrp: 1.758 ± 0.309
2.01AspTyr: 2.01 ± 0.307
0.0AspXaa: 0.0 ± 0.0
Glu
6.091GluAla: 6.091 ± 0.739
0.377GluCys: 0.377 ± 0.184
4.459GluAsp: 4.459 ± 0.613
5.84GluGlu: 5.84 ± 0.831
2.261GluPhe: 2.261 ± 0.458
3.831GluGly: 3.831 ± 0.499
1.821GluHis: 1.821 ± 0.373
3.705GluIle: 3.705 ± 0.52
2.7GluLys: 2.7 ± 0.408
6.782GluLeu: 6.782 ± 0.587
1.696GluMet: 1.696 ± 0.352
1.758GluAsn: 1.758 ± 0.384
2.826GluPro: 2.826 ± 0.431
2.386GluGln: 2.386 ± 0.447
3.517GluArg: 3.517 ± 0.527
3.893GluSer: 3.893 ± 0.694
3.642GluThr: 3.642 ± 0.49
5.338GluVal: 5.338 ± 0.603
1.696GluTrp: 1.696 ± 0.32
2.072GluTyr: 2.072 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.7PheAla: 2.7 ± 0.498
0.314PheCys: 0.314 ± 0.155
2.952PheAsp: 2.952 ± 0.373
2.261PheGlu: 2.261 ± 0.333
0.691PhePhe: 0.691 ± 0.203
3.454PheGly: 3.454 ± 0.429
0.816PheHis: 0.816 ± 0.287
1.444PheIle: 1.444 ± 0.289
1.319PheLys: 1.319 ± 0.345
2.826PheLeu: 2.826 ± 0.482
0.628PheMet: 0.628 ± 0.223
1.507PheAsn: 1.507 ± 0.305
1.758PhePro: 1.758 ± 0.33
1.068PheGln: 1.068 ± 0.292
1.821PheArg: 1.821 ± 0.373
1.758PheSer: 1.758 ± 0.381
1.947PheThr: 1.947 ± 0.381
1.821PheVal: 1.821 ± 0.32
0.628PheTrp: 0.628 ± 0.203
1.068PheTyr: 1.068 ± 0.27
0.0PheXaa: 0.0 ± 0.0
Gly
7.347GlyAla: 7.347 ± 1.01
0.754GlyCys: 0.754 ± 0.255
5.526GlyAsp: 5.526 ± 0.606
4.396GlyGlu: 4.396 ± 0.458
2.826GlyPhe: 2.826 ± 0.406
9.734GlyGly: 9.734 ± 2.274
1.633GlyHis: 1.633 ± 0.28
4.835GlyIle: 4.835 ± 0.832
3.642GlyLys: 3.642 ± 0.51
7.285GlyLeu: 7.285 ± 0.918
1.884GlyMet: 1.884 ± 0.305
3.014GlyAsn: 3.014 ± 0.404
3.768GlyPro: 3.768 ± 0.546
2.198GlyGln: 2.198 ± 0.321
4.459GlyArg: 4.459 ± 0.601
6.594GlySer: 6.594 ± 0.927
4.773GlyThr: 4.773 ± 0.693
5.715GlyVal: 5.715 ± 0.575
3.014GlyTrp: 3.014 ± 0.446
3.14GlyTyr: 3.14 ± 0.461
0.0GlyXaa: 0.0 ± 0.0
His
1.947HisAla: 1.947 ± 0.374
0.188HisCys: 0.188 ± 0.147
1.068HisAsp: 1.068 ± 0.204
1.696HisGlu: 1.696 ± 0.345
0.754HisPhe: 0.754 ± 0.204
1.319HisGly: 1.319 ± 0.317
0.565HisHis: 0.565 ± 0.167
0.816HisIle: 0.816 ± 0.219
0.942HisLys: 0.942 ± 0.285
1.13HisLeu: 1.13 ± 0.353
0.126HisMet: 0.126 ± 0.129
0.377HisAsn: 0.377 ± 0.17
1.193HisPro: 1.193 ± 0.236
0.942HisGln: 0.942 ± 0.299
1.633HisArg: 1.633 ± 0.35
0.691HisSer: 0.691 ± 0.211
1.13HisThr: 1.13 ± 0.313
1.696HisVal: 1.696 ± 0.331
0.565HisTrp: 0.565 ± 0.184
0.565HisTyr: 0.565 ± 0.209
0.0HisXaa: 0.0 ± 0.0
Ile
5.777IleAla: 5.777 ± 0.724
0.188IleCys: 0.188 ± 0.093
3.328IleAsp: 3.328 ± 0.435
3.831IleGlu: 3.831 ± 0.533
1.005IlePhe: 1.005 ± 0.208
4.082IleGly: 4.082 ± 0.492
0.816IleHis: 0.816 ± 0.228
2.01IleIle: 2.01 ± 0.315
2.198IleLys: 2.198 ± 0.369
3.077IleLeu: 3.077 ± 0.392
0.816IleMet: 0.816 ± 0.211
1.947IleAsn: 1.947 ± 0.321
3.14IlePro: 3.14 ± 0.407
1.507IleGln: 1.507 ± 0.391
3.203IleArg: 3.203 ± 0.459
3.705IleSer: 3.705 ± 0.486
3.956IleThr: 3.956 ± 0.445
3.077IleVal: 3.077 ± 0.526
0.691IleTrp: 0.691 ± 0.21
1.758IleTyr: 1.758 ± 0.269
0.0IleXaa: 0.0 ± 0.0
Lys
3.705LysAla: 3.705 ± 0.5
0.377LysCys: 0.377 ± 0.14
2.638LysAsp: 2.638 ± 0.443
2.072LysGlu: 2.072 ± 0.359
1.633LysPhe: 1.633 ± 0.337
2.512LysGly: 2.512 ± 0.434
1.13LysHis: 1.13 ± 0.32
2.449LysIle: 2.449 ± 0.372
2.072LysLys: 2.072 ± 0.427
3.58LysLeu: 3.58 ± 0.484
1.13LysMet: 1.13 ± 0.197
1.57LysAsn: 1.57 ± 0.316
2.826LysPro: 2.826 ± 0.454
2.198LysGln: 2.198 ± 0.474
3.391LysArg: 3.391 ± 0.619
2.7LysSer: 2.7 ± 0.451
2.449LysThr: 2.449 ± 0.363
2.952LysVal: 2.952 ± 0.465
0.628LysTrp: 0.628 ± 0.217
0.942LysTyr: 0.942 ± 0.238
0.0LysXaa: 0.0 ± 0.0
Leu
9.294LeuAla: 9.294 ± 0.991
0.251LeuCys: 0.251 ± 0.127
6.405LeuAsp: 6.405 ± 0.623
5.149LeuGlu: 5.149 ± 0.638
2.449LeuPhe: 2.449 ± 0.383
7.285LeuGly: 7.285 ± 0.745
1.507LeuHis: 1.507 ± 0.296
4.71LeuIle: 4.71 ± 0.518
4.521LeuLys: 4.521 ± 0.51
5.652LeuLeu: 5.652 ± 0.628
1.821LeuMet: 1.821 ± 0.277
2.7LeuAsn: 2.7 ± 0.385
5.966LeuPro: 5.966 ± 0.687
2.826LeuGln: 2.826 ± 0.513
5.777LeuArg: 5.777 ± 0.561
5.652LeuSer: 5.652 ± 0.54
5.338LeuThr: 5.338 ± 0.495
4.333LeuVal: 4.333 ± 0.677
1.256LeuTrp: 1.256 ± 0.36
1.947LeuTyr: 1.947 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
2.512MetAla: 2.512 ± 0.313
0.0MetCys: 0.0 ± 0.0
1.193MetAsp: 1.193 ± 0.277
1.256MetGlu: 1.256 ± 0.278
0.691MetPhe: 0.691 ± 0.17
1.633MetGly: 1.633 ± 0.269
0.377MetHis: 0.377 ± 0.157
0.628MetIle: 0.628 ± 0.196
1.193MetLys: 1.193 ± 0.29
1.13MetLeu: 1.13 ± 0.258
0.188MetMet: 0.188 ± 0.107
1.005MetAsn: 1.005 ± 0.231
1.256MetPro: 1.256 ± 0.259
0.251MetGln: 0.251 ± 0.124
1.256MetArg: 1.256 ± 0.297
2.01MetSer: 2.01 ± 0.361
2.386MetThr: 2.386 ± 0.29
1.13MetVal: 1.13 ± 0.278
0.188MetTrp: 0.188 ± 0.113
0.502MetTyr: 0.502 ± 0.193
0.0MetXaa: 0.0 ± 0.0
Asn
3.328AsnAla: 3.328 ± 0.499
0.126AsnCys: 0.126 ± 0.099
2.01AsnAsp: 2.01 ± 0.356
1.947AsnGlu: 1.947 ± 0.366
1.13AsnPhe: 1.13 ± 0.259
3.266AsnGly: 3.266 ± 0.473
0.816AsnHis: 0.816 ± 0.245
1.57AsnIle: 1.57 ± 0.417
0.754AsnLys: 0.754 ± 0.254
2.512AsnLeu: 2.512 ± 0.34
0.565AsnMet: 0.565 ± 0.183
0.754AsnAsn: 0.754 ± 0.207
2.638AsnPro: 2.638 ± 0.327
1.193AsnGln: 1.193 ± 0.279
1.256AsnArg: 1.256 ± 0.304
1.696AsnSer: 1.696 ± 0.395
1.947AsnThr: 1.947 ± 0.348
2.638AsnVal: 2.638 ± 0.369
0.816AsnTrp: 0.816 ± 0.221
1.382AsnTyr: 1.382 ± 0.261
0.0AsnXaa: 0.0 ± 0.0
Pro
5.024ProAla: 5.024 ± 0.535
0.502ProCys: 0.502 ± 0.201
4.396ProAsp: 4.396 ± 0.52
4.835ProGlu: 4.835 ± 0.625
2.324ProPhe: 2.324 ± 0.392
5.024ProGly: 5.024 ± 0.541
0.879ProHis: 0.879 ± 0.229
2.449ProIle: 2.449 ± 0.387
2.01ProLys: 2.01 ± 0.274
4.584ProLeu: 4.584 ± 0.574
1.005ProMet: 1.005 ± 0.229
1.696ProAsn: 1.696 ± 0.323
2.826ProPro: 2.826 ± 0.461
1.696ProGln: 1.696 ± 0.363
2.952ProArg: 2.952 ± 0.546
3.768ProSer: 3.768 ± 0.483
3.391ProThr: 3.391 ± 0.472
3.956ProVal: 3.956 ± 0.548
0.879ProTrp: 0.879 ± 0.285
1.57ProTyr: 1.57 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
3.14GlnAla: 3.14 ± 0.544
0.063GlnCys: 0.063 ± 0.062
1.382GlnAsp: 1.382 ± 0.389
1.696GlnGlu: 1.696 ± 0.332
1.444GlnPhe: 1.444 ± 0.286
2.449GlnGly: 2.449 ± 0.347
0.565GlnHis: 0.565 ± 0.154
2.889GlnIle: 2.889 ± 0.541
1.382GlnLys: 1.382 ± 0.336
3.893GlnLeu: 3.893 ± 0.439
1.005GlnMet: 1.005 ± 0.263
0.502GlnAsn: 0.502 ± 0.18
2.01GlnPro: 2.01 ± 0.364
1.821GlnGln: 1.821 ± 0.413
1.696GlnArg: 1.696 ± 0.363
1.57GlnSer: 1.57 ± 0.315
1.758GlnThr: 1.758 ± 0.351
2.01GlnVal: 2.01 ± 0.295
0.691GlnTrp: 0.691 ± 0.17
0.565GlnTyr: 0.565 ± 0.17
0.0GlnXaa: 0.0 ± 0.0
Arg
5.715ArgAla: 5.715 ± 0.688
0.628ArgCys: 0.628 ± 0.236
2.763ArgAsp: 2.763 ± 0.394
4.333ArgGlu: 4.333 ± 0.57
1.507ArgPhe: 1.507 ± 0.303
4.961ArgGly: 4.961 ± 0.767
0.816ArgHis: 0.816 ± 0.214
3.203ArgIle: 3.203 ± 0.465
2.952ArgLys: 2.952 ± 0.494
5.338ArgLeu: 5.338 ± 0.74
1.633ArgMet: 1.633 ± 0.397
2.324ArgAsn: 2.324 ± 0.436
2.638ArgPro: 2.638 ± 0.438
2.01ArgGln: 2.01 ± 0.376
4.647ArgArg: 4.647 ± 0.684
3.454ArgSer: 3.454 ± 0.488
3.203ArgThr: 3.203 ± 0.562
4.773ArgVal: 4.773 ± 0.481
1.444ArgTrp: 1.444 ± 0.306
1.947ArgTyr: 1.947 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
7.033SerAla: 7.033 ± 0.937
0.502SerCys: 0.502 ± 0.186
3.203SerAsp: 3.203 ± 0.35
3.58SerGlu: 3.58 ± 0.435
2.135SerPhe: 2.135 ± 0.424
6.908SerGly: 6.908 ± 0.955
1.256SerHis: 1.256 ± 0.3
2.7SerIle: 2.7 ± 0.397
2.072SerLys: 2.072 ± 0.351
4.898SerLeu: 4.898 ± 0.489
1.444SerMet: 1.444 ± 0.272
2.324SerAsn: 2.324 ± 0.425
3.077SerPro: 3.077 ± 0.469
1.947SerGln: 1.947 ± 0.3
3.014SerArg: 3.014 ± 0.443
3.391SerSer: 3.391 ± 0.624
3.077SerThr: 3.077 ± 0.446
3.956SerVal: 3.956 ± 0.563
1.507SerTrp: 1.507 ± 0.389
1.507SerTyr: 1.507 ± 0.307
0.0SerXaa: 0.0 ± 0.0
Thr
6.091ThrAla: 6.091 ± 0.787
0.251ThrCys: 0.251 ± 0.118
4.145ThrAsp: 4.145 ± 0.57
4.71ThrGlu: 4.71 ± 0.475
2.01ThrPhe: 2.01 ± 0.429
6.28ThrGly: 6.28 ± 0.603
1.256ThrHis: 1.256 ± 0.306
2.638ThrIle: 2.638 ± 0.544
2.826ThrLys: 2.826 ± 0.34
5.652ThrLeu: 5.652 ± 0.722
0.816ThrMet: 0.816 ± 0.188
1.947ThrAsn: 1.947 ± 0.41
3.517ThrPro: 3.517 ± 0.472
1.507ThrGln: 1.507 ± 0.329
3.454ThrArg: 3.454 ± 0.525
3.14ThrSer: 3.14 ± 0.601
3.831ThrThr: 3.831 ± 0.528
5.024ThrVal: 5.024 ± 0.563
1.13ThrTrp: 1.13 ± 0.297
1.758ThrTyr: 1.758 ± 0.399
0.0ThrXaa: 0.0 ± 0.0
Val
6.908ValAla: 6.908 ± 0.758
0.314ValCys: 0.314 ± 0.169
4.898ValAsp: 4.898 ± 0.641
4.71ValGlu: 4.71 ± 0.517
2.261ValPhe: 2.261 ± 0.348
4.961ValGly: 4.961 ± 0.604
1.444ValHis: 1.444 ± 0.286
3.642ValIle: 3.642 ± 0.494
3.077ValLys: 3.077 ± 0.522
5.401ValLeu: 5.401 ± 0.548
0.942ValMet: 0.942 ± 0.275
2.889ValAsn: 2.889 ± 0.352
4.27ValPro: 4.27 ± 0.57
2.324ValGln: 2.324 ± 0.466
4.835ValArg: 4.835 ± 0.662
4.082ValSer: 4.082 ± 0.49
5.212ValThr: 5.212 ± 0.642
4.773ValVal: 4.773 ± 0.694
1.193ValTrp: 1.193 ± 0.304
2.449ValTyr: 2.449 ± 0.397
0.0ValXaa: 0.0 ± 0.0
Trp
1.444TrpAla: 1.444 ± 0.342
0.251TrpCys: 0.251 ± 0.123
1.507TrpAsp: 1.507 ± 0.255
1.13TrpGlu: 1.13 ± 0.203
0.942TrpPhe: 0.942 ± 0.256
1.696TrpGly: 1.696 ± 0.317
0.44TrpHis: 0.44 ± 0.183
1.193TrpIle: 1.193 ± 0.235
0.44TrpLys: 0.44 ± 0.228
1.947TrpLeu: 1.947 ± 0.341
0.565TrpMet: 0.565 ± 0.199
0.377TrpAsn: 0.377 ± 0.129
1.005TrpPro: 1.005 ± 0.293
0.879TrpGln: 0.879 ± 0.222
1.444TrpArg: 1.444 ± 0.31
1.068TrpSer: 1.068 ± 0.218
1.633TrpThr: 1.633 ± 0.409
2.135TrpVal: 2.135 ± 0.37
0.502TrpTrp: 0.502 ± 0.183
0.377TrpTyr: 0.377 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.7TyrAla: 2.7 ± 0.428
0.251TyrCys: 0.251 ± 0.166
1.13TyrAsp: 1.13 ± 0.269
2.198TyrGlu: 2.198 ± 0.313
0.502TyrPhe: 0.502 ± 0.166
2.261TyrGly: 2.261 ± 0.404
0.628TyrHis: 0.628 ± 0.212
1.758TyrIle: 1.758 ± 0.369
1.256TyrLys: 1.256 ± 0.282
2.575TyrLeu: 2.575 ± 0.43
0.628TyrMet: 0.628 ± 0.177
1.319TyrAsn: 1.319 ± 0.244
1.633TyrPro: 1.633 ± 0.336
1.005TyrGln: 1.005 ± 0.308
2.449TyrArg: 2.449 ± 0.325
1.444TyrSer: 1.444 ± 0.302
2.198TyrThr: 2.198 ± 0.453
2.135TyrVal: 2.135 ± 0.34
0.314TyrTrp: 0.314 ± 0.135
0.565TyrTyr: 0.565 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (15925 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski