Rhizophagus sp. RF1 mitovirus
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2RWR3|E2RWR3_9VIRU RNA-directed RNA polymerase OS=Rhizophagus sp. RF1 mitovirus OX=758866 GN=RdRp PE=4 SV=2
MM1 pKa = 7.15 STFNFNSPNEE11 pKa = 4.04 VRR13 pKa = 11.84 RR14 pKa = 11.84 VTRR17 pKa = 11.84 SNIASLFAALMHH29 pKa = 6.55 LNSLVTVTYY38 pKa = 9.13 SQRR41 pKa = 11.84 LLAFLGAVYY50 pKa = 10.82 NRR52 pKa = 11.84 ILVLFDD58 pKa = 3.76 ANARR62 pKa = 11.84 SLISEE67 pKa = 4.59 LKK69 pKa = 10.32 LVRR72 pKa = 11.84 RR73 pKa = 11.84 WFLEE77 pKa = 4.45 FIRR80 pKa = 11.84 NGNTDD85 pKa = 3.39 NPGLEE90 pKa = 3.86 WDD92 pKa = 4.1 RR93 pKa = 11.84 WDD95 pKa = 5.14 DD96 pKa = 4.2 SNNCPVLLEE105 pKa = 4.52 GLDD108 pKa = 3.85 ALWDD112 pKa = 4.55 EE113 pKa = 4.94 IEE115 pKa = 4.56 SPSDD119 pKa = 3.12 SHH121 pKa = 5.98 YY122 pKa = 9.92 TDD124 pKa = 4.25 HH125 pKa = 7.42 AAQLIFTLLSIDD137 pKa = 4.15 RR138 pKa = 11.84 IIVVPAVPNYY148 pKa = 9.48 STIEE152 pKa = 4.35 DD153 pKa = 4.19 GPVIEE158 pKa = 4.36 PVGYY162 pKa = 6.95 PTNEE166 pKa = 3.85 EE167 pKa = 4.02 LVNALSSLHH176 pKa = 6.74 IDD178 pKa = 3.4 PAAFKK183 pKa = 10.73 AFYY186 pKa = 9.08 NQQVHH191 pKa = 6.66 DD192 pKa = 4.22 FDD194 pKa = 5.39 YY195 pKa = 11.22 EE196 pKa = 4.3 VLSTRR201 pKa = 11.84 GPNGDD206 pKa = 3.94 ATWTAHH212 pKa = 6.22 LDD214 pKa = 3.31 ARR216 pKa = 11.84 AWALDD221 pKa = 3.42 TEE223 pKa = 4.5 LFRR226 pKa = 11.84 RR227 pKa = 11.84 FSAWLEE233 pKa = 3.76 EE234 pKa = 4.16 SRR236 pKa = 11.84 LTRR239 pKa = 11.84 ILRR242 pKa = 11.84 DD243 pKa = 3.17 LFGCIRR249 pKa = 11.84 SAAAEE254 pKa = 4.66 AIPNLSPILGKK265 pKa = 10.73 LSVIEE270 pKa = 4.01 EE271 pKa = 4.27 WGGKK275 pKa = 9.01 ARR277 pKa = 11.84 IVAQMDD283 pKa = 3.68 YY284 pKa = 7.44 WTQMALTPLHH294 pKa = 5.34 NTINHH299 pKa = 6.63 FLRR302 pKa = 11.84 ALKK305 pKa = 10.43 EE306 pKa = 4.21 DD307 pKa = 3.65 GTFNQHH313 pKa = 7.04 AIAEE317 pKa = 4.48 RR318 pKa = 11.84 VRR320 pKa = 11.84 QWTADD325 pKa = 3.04 PSMEE329 pKa = 4.14 VFSFDD334 pKa = 3.07 LTAATDD340 pKa = 3.63 RR341 pKa = 11.84 VPITFQEE348 pKa = 4.57 SILSYY353 pKa = 11.4 LMTSKK358 pKa = 10.86 SFGNGWASILVDD370 pKa = 4.94 RR371 pKa = 11.84 EE372 pKa = 3.87 FLTPNGDD379 pKa = 3.92 LISYY383 pKa = 7.6 NTGQPMGARR392 pKa = 11.84 SSFPMLALTHH402 pKa = 6.48 HH403 pKa = 7.23 IIVQIAAARR412 pKa = 11.84 AGLTVYY418 pKa = 10.44 RR419 pKa = 11.84 DD420 pKa = 3.73 YY421 pKa = 11.74 VVLGDD426 pKa = 6.02 DD427 pKa = 3.61 VTLTNAQVAAHH438 pKa = 6.11 YY439 pKa = 7.21 QTIMRR444 pKa = 11.84 CLGVPINLSKK454 pKa = 11.05 SIVHH458 pKa = 5.82 VDD460 pKa = 3.32 GGVSMAEE467 pKa = 3.23 ICKK470 pKa = 9.85 RR471 pKa = 11.84 VFMDD475 pKa = 3.91 GVEE478 pKa = 3.91 ISRR481 pKa = 11.84 FNPKK485 pKa = 10.33 LIVNVIRR492 pKa = 11.84 DD493 pKa = 3.74 GRR495 pKa = 11.84 LGPDD499 pKa = 3.7 LQNDD503 pKa = 4.77 LIIRR507 pKa = 11.84 GWDD510 pKa = 3.29 PSNEE514 pKa = 4.14 VFWKK518 pKa = 10.25 FMAGLLSIDD527 pKa = 4.01 NLTLLIRR534 pKa = 11.84 LNCAPISITGLLRR547 pKa = 11.84 QFASNSKK554 pKa = 9.73 LAQLSAWIPAYY565 pKa = 10.19 QDD567 pKa = 3.69 LKK569 pKa = 10.82 PEE571 pKa = 4.19 HH572 pKa = 6.55 LVEE575 pKa = 4.29 LFTYY579 pKa = 8.26 VTASEE584 pKa = 3.96 ALKK587 pKa = 10.76 RR588 pKa = 11.84 LDD590 pKa = 4.43 GILRR594 pKa = 11.84 AAVTINDD601 pKa = 3.94 SLSIIAAANAHH612 pKa = 7.13 PDD614 pKa = 4.05 RR615 pKa = 11.84 IPQYY619 pKa = 11.31 VRR621 pKa = 11.84 DD622 pKa = 3.61 TWLGEE627 pKa = 3.86 GLTKK631 pKa = 10.28 EE632 pKa = 3.75 EE633 pKa = 4.12 RR634 pKa = 11.84 ARR636 pKa = 11.84 LEE638 pKa = 3.79 GLIASTGPITPNHH651 pKa = 6.42 PLVSASRR658 pKa = 11.84 AEE660 pKa = 3.93 ANRR663 pKa = 11.84 ISEE666 pKa = 4.98 LLHH669 pKa = 5.75 QLNSHH674 pKa = 6.45 DD675 pKa = 3.57 TAIITRR681 pKa = 11.84 ARR683 pKa = 11.84 LGLLDD688 pKa = 3.58 VFRR691 pKa = 11.84 TSISSIWLDD700 pKa = 3.82 DD701 pKa = 3.82 GNIRR705 pKa = 11.84 AGEE708 pKa = 4.02 SRR710 pKa = 11.84 SIFTRR715 pKa = 11.84 MLTTLVSLFTSEE727 pKa = 4.23 KK728 pKa = 9.76 RR729 pKa = 11.84 VSKK732 pKa = 10.52 SGRR735 pKa = 11.84 NLSLSYY741 pKa = 10.88 SVVLTSLSRR750 pKa = 11.84 LWTVALDD757 pKa = 3.61 FGGQVTVNALRR768 pKa = 11.84 ANVTRR773 pKa = 11.84 DD774 pKa = 2.63 IHH776 pKa = 6.35 NAVDD780 pKa = 3.5 NLKK783 pKa = 10.35 AAEE786 pKa = 4.0 EE787 pKa = 4.01 AAVLISSSSVPTTSPTPATPKK808 pKa = 9.6 GIRR811 pKa = 11.84 RR812 pKa = 11.84 RR813 pKa = 11.84 RR814 pKa = 11.84 AFARR818 pKa = 11.84 ISS820 pKa = 3.22
Molecular weight: 91.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.99
IPC2_protein 5.842
IPC_protein 5.919
Toseland 6.389
ProMoST 6.275
Dawson 6.198
Bjellqvist 6.198
Wikipedia 6.224
Rodwell 6.186
Grimsley 6.576
Solomon 6.198
Lehninger 6.198
Nozaki 6.478
DTASelect 6.664
Thurlkill 6.678
EMBOSS 6.649
Sillero 6.576
Patrickios 4.482
IPC_peptide 6.224
IPC2_peptide 6.576
IPC2.peptide.svr19 6.497
Protein with the highest isoelectric point:
>tr|E2RWR3|E2RWR3_9VIRU RNA-directed RNA polymerase OS=Rhizophagus sp. RF1 mitovirus OX=758866 GN=RdRp PE=4 SV=2
MM1 pKa = 7.15 STFNFNSPNEE11 pKa = 4.04 VRR13 pKa = 11.84 RR14 pKa = 11.84 VTRR17 pKa = 11.84 SNIASLFAALMHH29 pKa = 6.55 LNSLVTVTYY38 pKa = 9.13 SQRR41 pKa = 11.84 LLAFLGAVYY50 pKa = 10.82 NRR52 pKa = 11.84 ILVLFDD58 pKa = 3.76 ANARR62 pKa = 11.84 SLISEE67 pKa = 4.59 LKK69 pKa = 10.32 LVRR72 pKa = 11.84 RR73 pKa = 11.84 WFLEE77 pKa = 4.45 FIRR80 pKa = 11.84 NGNTDD85 pKa = 3.39 NPGLEE90 pKa = 3.86 WDD92 pKa = 4.1 RR93 pKa = 11.84 WDD95 pKa = 5.14 DD96 pKa = 4.2 SNNCPVLLEE105 pKa = 4.52 GLDD108 pKa = 3.85 ALWDD112 pKa = 4.55 EE113 pKa = 4.94 IEE115 pKa = 4.56 SPSDD119 pKa = 3.12 SHH121 pKa = 5.98 YY122 pKa = 9.92 TDD124 pKa = 4.25 HH125 pKa = 7.42 AAQLIFTLLSIDD137 pKa = 4.15 RR138 pKa = 11.84 IIVVPAVPNYY148 pKa = 9.48 STIEE152 pKa = 4.35 DD153 pKa = 4.19 GPVIEE158 pKa = 4.36 PVGYY162 pKa = 6.95 PTNEE166 pKa = 3.85 EE167 pKa = 4.02 LVNALSSLHH176 pKa = 6.74 IDD178 pKa = 3.4 PAAFKK183 pKa = 10.73 AFYY186 pKa = 9.08 NQQVHH191 pKa = 6.66 DD192 pKa = 4.22 FDD194 pKa = 5.39 YY195 pKa = 11.22 EE196 pKa = 4.3 VLSTRR201 pKa = 11.84 GPNGDD206 pKa = 3.94 ATWTAHH212 pKa = 6.22 LDD214 pKa = 3.31 ARR216 pKa = 11.84 AWALDD221 pKa = 3.42 TEE223 pKa = 4.5 LFRR226 pKa = 11.84 RR227 pKa = 11.84 FSAWLEE233 pKa = 3.76 EE234 pKa = 4.16 SRR236 pKa = 11.84 LTRR239 pKa = 11.84 ILRR242 pKa = 11.84 DD243 pKa = 3.17 LFGCIRR249 pKa = 11.84 SAAAEE254 pKa = 4.66 AIPNLSPILGKK265 pKa = 10.73 LSVIEE270 pKa = 4.01 EE271 pKa = 4.27 WGGKK275 pKa = 9.01 ARR277 pKa = 11.84 IVAQMDD283 pKa = 3.68 YY284 pKa = 7.44 WTQMALTPLHH294 pKa = 5.34 NTINHH299 pKa = 6.63 FLRR302 pKa = 11.84 ALKK305 pKa = 10.43 EE306 pKa = 4.21 DD307 pKa = 3.65 GTFNQHH313 pKa = 7.04 AIAEE317 pKa = 4.48 RR318 pKa = 11.84 VRR320 pKa = 11.84 QWTADD325 pKa = 3.04 PSMEE329 pKa = 4.14 VFSFDD334 pKa = 3.07 LTAATDD340 pKa = 3.63 RR341 pKa = 11.84 VPITFQEE348 pKa = 4.57 SILSYY353 pKa = 11.4 LMTSKK358 pKa = 10.86 SFGNGWASILVDD370 pKa = 4.94 RR371 pKa = 11.84 EE372 pKa = 3.87 FLTPNGDD379 pKa = 3.92 LISYY383 pKa = 7.6 NTGQPMGARR392 pKa = 11.84 SSFPMLALTHH402 pKa = 6.48 HH403 pKa = 7.23 IIVQIAAARR412 pKa = 11.84 AGLTVYY418 pKa = 10.44 RR419 pKa = 11.84 DD420 pKa = 3.73 YY421 pKa = 11.74 VVLGDD426 pKa = 6.02 DD427 pKa = 3.61 VTLTNAQVAAHH438 pKa = 6.11 YY439 pKa = 7.21 QTIMRR444 pKa = 11.84 CLGVPINLSKK454 pKa = 11.05 SIVHH458 pKa = 5.82 VDD460 pKa = 3.32 GGVSMAEE467 pKa = 3.23 ICKK470 pKa = 9.85 RR471 pKa = 11.84 VFMDD475 pKa = 3.91 GVEE478 pKa = 3.91 ISRR481 pKa = 11.84 FNPKK485 pKa = 10.33 LIVNVIRR492 pKa = 11.84 DD493 pKa = 3.74 GRR495 pKa = 11.84 LGPDD499 pKa = 3.7 LQNDD503 pKa = 4.77 LIIRR507 pKa = 11.84 GWDD510 pKa = 3.29 PSNEE514 pKa = 4.14 VFWKK518 pKa = 10.25 FMAGLLSIDD527 pKa = 4.01 NLTLLIRR534 pKa = 11.84 LNCAPISITGLLRR547 pKa = 11.84 QFASNSKK554 pKa = 9.73 LAQLSAWIPAYY565 pKa = 10.19 QDD567 pKa = 3.69 LKK569 pKa = 10.82 PEE571 pKa = 4.19 HH572 pKa = 6.55 LVEE575 pKa = 4.29 LFTYY579 pKa = 8.26 VTASEE584 pKa = 3.96 ALKK587 pKa = 10.76 RR588 pKa = 11.84 LDD590 pKa = 4.43 GILRR594 pKa = 11.84 AAVTINDD601 pKa = 3.94 SLSIIAAANAHH612 pKa = 7.13 PDD614 pKa = 4.05 RR615 pKa = 11.84 IPQYY619 pKa = 11.31 VRR621 pKa = 11.84 DD622 pKa = 3.61 TWLGEE627 pKa = 3.86 GLTKK631 pKa = 10.28 EE632 pKa = 3.75 EE633 pKa = 4.12 RR634 pKa = 11.84 ARR636 pKa = 11.84 LEE638 pKa = 3.79 GLIASTGPITPNHH651 pKa = 6.42 PLVSASRR658 pKa = 11.84 AEE660 pKa = 3.93 ANRR663 pKa = 11.84 ISEE666 pKa = 4.98 LLHH669 pKa = 5.75 QLNSHH674 pKa = 6.45 DD675 pKa = 3.57 TAIITRR681 pKa = 11.84 ARR683 pKa = 11.84 LGLLDD688 pKa = 3.58 VFRR691 pKa = 11.84 TSISSIWLDD700 pKa = 3.82 DD701 pKa = 3.82 GNIRR705 pKa = 11.84 AGEE708 pKa = 4.02 SRR710 pKa = 11.84 SIFTRR715 pKa = 11.84 MLTTLVSLFTSEE727 pKa = 4.23 KK728 pKa = 9.76 RR729 pKa = 11.84 VSKK732 pKa = 10.52 SGRR735 pKa = 11.84 NLSLSYY741 pKa = 10.88 SVVLTSLSRR750 pKa = 11.84 LWTVALDD757 pKa = 3.61 FGGQVTVNALRR768 pKa = 11.84 ANVTRR773 pKa = 11.84 DD774 pKa = 2.63 IHH776 pKa = 6.35 NAVDD780 pKa = 3.5 NLKK783 pKa = 10.35 AAEE786 pKa = 4.0 EE787 pKa = 4.01 AAVLISSSSVPTTSPTPATPKK808 pKa = 9.6 GIRR811 pKa = 11.84 RR812 pKa = 11.84 RR813 pKa = 11.84 RR814 pKa = 11.84 AFARR818 pKa = 11.84 ISS820 pKa = 3.22
Molecular weight: 91.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.99
IPC2_protein 5.842
IPC_protein 5.919
Toseland 6.389
ProMoST 6.275
Dawson 6.198
Bjellqvist 6.198
Wikipedia 6.224
Rodwell 6.186
Grimsley 6.576
Solomon 6.198
Lehninger 6.198
Nozaki 6.478
DTASelect 6.664
Thurlkill 6.678
EMBOSS 6.649
Sillero 6.576
Patrickios 4.482
IPC_peptide 6.224
IPC2_peptide 6.576
IPC2.peptide.svr19 6.497
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
820
820
820
820.0
91.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.512 ± 0.0
0.61 ± 0.0
5.732 ± 0.0
4.756 ± 0.0
4.024 ± 0.0
5.0 ± 0.0
2.317 ± 0.0
7.195 ± 0.0
2.195 ± 0.0
11.829 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.585 ± 0.0
5.366 ± 0.0
4.268 ± 0.0
2.439 ± 0.0
7.317 ± 0.0
8.537 ± 0.0
6.585 ± 0.0
6.585 ± 0.0
2.073 ± 0.0
2.073 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here