Klebsiella phage KMI1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Przondovirus; unclassified Przondovirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514U6C1|A0A514U6C1_9CAUD Uncharacterized protein OS=Klebsiella phage KMI1 OX=2589653 GN=KMI1_25 PE=4 SV=1
MM1 pKa = 7.38NMQDD5 pKa = 4.11AYY7 pKa = 10.62FGSAAEE13 pKa = 4.04LDD15 pKa = 4.97AINEE19 pKa = 3.9MLAAIGEE26 pKa = 4.7SPVTTLDD33 pKa = 3.33EE34 pKa = 5.18DD35 pKa = 4.25GSADD39 pKa = 3.27VANARR44 pKa = 11.84RR45 pKa = 11.84ILNRR49 pKa = 11.84INRR52 pKa = 11.84QIQSKK57 pKa = 9.61GWAFNINTAATLTPDD72 pKa = 3.38VDD74 pKa = 3.43TGLIPFRR81 pKa = 11.84PAYY84 pKa = 10.44LSILGGQYY92 pKa = 10.17VNRR95 pKa = 11.84GGWVYY100 pKa = 11.2DD101 pKa = 3.54KK102 pKa = 11.07STGTDD107 pKa = 3.14TFSGPITVTLITLQDD122 pKa = 3.68YY123 pKa = 11.66DD124 pKa = 4.32EE125 pKa = 4.72MPEE128 pKa = 5.08CFRR131 pKa = 11.84QWIVTKK137 pKa = 10.66ASRR140 pKa = 11.84QFNSRR145 pKa = 11.84FFGAEE150 pKa = 3.73DD151 pKa = 3.76VEE153 pKa = 4.46NSLAQEE159 pKa = 3.88EE160 pKa = 4.75MEE162 pKa = 5.66ARR164 pKa = 11.84MACNEE169 pKa = 3.84YY170 pKa = 10.91EE171 pKa = 4.14MDD173 pKa = 3.84FGQYY177 pKa = 11.01NMLDD181 pKa = 3.06GDD183 pKa = 4.58AYY185 pKa = 10.18VQGLIGRR192 pKa = 4.48

Molecular weight: 21.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514U6F1|A0A514U6F1_9CAUD Uncharacterized protein OS=Klebsiella phage KMI1 OX=2589653 GN=KMI1_2 PE=4 SV=1
MM1 pKa = 7.38LRR3 pKa = 11.84NMARR7 pKa = 11.84GIITAGLARR16 pKa = 11.84LAIWRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84KK24 pKa = 8.64MVHH27 pKa = 6.82CDD29 pKa = 3.58TPHH32 pKa = 6.21SPASEE37 pKa = 4.12EE38 pKa = 4.31LRR40 pKa = 11.84TTQKK44 pKa = 10.67RR45 pKa = 11.84KK46 pKa = 9.72YY47 pKa = 8.92KK48 pKa = 9.34YY49 pKa = 10.17SKK51 pKa = 9.59SAPSGVVSATGKK63 pKa = 9.31PIGYY67 pKa = 8.91KK68 pKa = 10.35RR69 pKa = 11.84KK70 pKa = 9.99DD71 pKa = 3.74GYY73 pKa = 10.32WLLSYY78 pKa = 10.49GGKK81 pKa = 9.42HH82 pKa = 5.75LLSHH86 pKa = 6.21RR87 pKa = 11.84VVWMLNFGVIPEE99 pKa = 4.37GLVIDD104 pKa = 5.35HH105 pKa = 7.17INRR108 pKa = 11.84DD109 pKa = 3.33PSDD112 pKa = 3.52NRR114 pKa = 11.84IEE116 pKa = 4.06NLRR119 pKa = 11.84AVTTSANNANSRR131 pKa = 11.84VRR133 pKa = 11.84SHH135 pKa = 6.47CVSGEE140 pKa = 3.78KK141 pKa = 10.51NIAVDD146 pKa = 3.16KK147 pKa = 9.2RR148 pKa = 11.84TGRR151 pKa = 11.84FTVRR155 pKa = 11.84ISRR158 pKa = 11.84KK159 pKa = 9.65SYY161 pKa = 8.52GTYY164 pKa = 9.08GTLAEE169 pKa = 5.02AIAVRR174 pKa = 11.84DD175 pKa = 3.9ANDD178 pKa = 2.89

Molecular weight: 19.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

41

0

41

11895

67

1321

290.1

32.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.928 ± 0.485

0.916 ± 0.152

6.297 ± 0.243

6.818 ± 0.463

3.573 ± 0.162

8.113 ± 0.344

1.799 ± 0.205

4.952 ± 0.207

6.49 ± 0.387

8.045 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.53 ± 0.172

4.161 ± 0.249

3.691 ± 0.21

4.128 ± 0.3

5.523 ± 0.242

6.49 ± 0.362

5.834 ± 0.281

6.969 ± 0.375

1.295 ± 0.164

3.447 ± 0.217
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski