Tortoise microvirus 24

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6Z9|A0A4P8W6Z9_9VIRU Replication initiation protein OS=Tortoise microvirus 24 OX=2583126 PE=4 SV=1
MM1 pKa = 7.95DD2 pKa = 5.28NDD4 pKa = 3.87YY5 pKa = 11.18LYY7 pKa = 10.53TIYY10 pKa = 11.04DD11 pKa = 3.63RR12 pKa = 11.84VAEE15 pKa = 4.19EE16 pKa = 4.33GFPPFVARR24 pKa = 11.84TDD26 pKa = 3.58GVAIRR31 pKa = 11.84QFMASLAKK39 pKa = 10.15IEE41 pKa = 4.35NPGFNPDD48 pKa = 3.13EE49 pKa = 4.19FVLYY53 pKa = 10.28RR54 pKa = 11.84VGTWNSRR61 pKa = 11.84SMVIEE66 pKa = 4.19PLDD69 pKa = 3.76PEE71 pKa = 4.51QIVVTPRR78 pKa = 11.84EE79 pKa = 4.05QVEE82 pKa = 4.27VV83 pKa = 3.56

Molecular weight: 9.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9D0|A0A4P8W9D0_9VIRU Uncharacterized protein OS=Tortoise microvirus 24 OX=2583126 PE=4 SV=1
MM1 pKa = 7.83VDD3 pKa = 3.18EE4 pKa = 4.81GLVDD8 pKa = 3.75EE9 pKa = 5.45EE10 pKa = 4.65GTGDD14 pKa = 4.27VEE16 pKa = 5.14CTSKK20 pKa = 10.45WKK22 pKa = 10.45IKK24 pKa = 9.07TGQLVRR30 pKa = 11.84CGVCMSCRR38 pKa = 11.84ITRR41 pKa = 11.84TEE43 pKa = 3.55EE44 pKa = 3.01WAMRR48 pKa = 11.84IEE50 pKa = 4.43HH51 pKa = 6.36EE52 pKa = 4.35CHH54 pKa = 6.14GKK56 pKa = 9.7EE57 pKa = 4.1GCFITLTYY65 pKa = 10.33RR66 pKa = 11.84DD67 pKa = 4.77DD68 pKa = 5.58PITLNKK74 pKa = 9.78RR75 pKa = 11.84DD76 pKa = 3.53LQLFFKK82 pKa = 10.62RR83 pKa = 11.84LRR85 pKa = 11.84RR86 pKa = 11.84RR87 pKa = 11.84LDD89 pKa = 3.31VPIKK93 pKa = 10.38YY94 pKa = 8.73YY95 pKa = 11.13ACGEE99 pKa = 4.12YY100 pKa = 10.9GDD102 pKa = 4.98LNSRR106 pKa = 11.84PHH108 pKa = 4.85YY109 pKa = 9.81HH110 pKa = 7.21AIIIGWRR117 pKa = 11.84PMDD120 pKa = 4.23LLKK123 pKa = 10.21TGKK126 pKa = 9.93NYY128 pKa = 9.3RR129 pKa = 11.84SKK131 pKa = 10.21MIEE134 pKa = 4.08EE135 pKa = 4.47LWPFGFNVVGSIDD148 pKa = 3.84PKK150 pKa = 9.7SIRR153 pKa = 11.84YY154 pKa = 4.64VTGYY158 pKa = 9.56IRR160 pKa = 11.84KK161 pKa = 9.24KK162 pKa = 10.7LYY164 pKa = 9.61GHH166 pKa = 7.43AAAEE170 pKa = 4.32YY171 pKa = 10.43GDD173 pKa = 4.03RR174 pKa = 11.84LPPFSLMSVGIGKK187 pKa = 10.02EE188 pKa = 3.81YY189 pKa = 11.01ALNNAEE195 pKa = 4.2RR196 pKa = 11.84LSKK199 pKa = 11.12NLMQTRR205 pKa = 11.84NGKK208 pKa = 7.1TVGLPRR214 pKa = 11.84YY215 pKa = 9.5YY216 pKa = 10.62KK217 pKa = 10.3EE218 pKa = 4.76LYY220 pKa = 10.37DD221 pKa = 4.32RR222 pKa = 11.84DD223 pKa = 3.86GAQVTSRR230 pKa = 11.84VRR232 pKa = 11.84WSTKK236 pKa = 8.49LSRR239 pKa = 11.84VKK241 pKa = 10.32KK242 pKa = 9.06VKK244 pKa = 10.28EE245 pKa = 3.86YY246 pKa = 10.9LDD248 pKa = 3.43SDD250 pKa = 3.5RR251 pKa = 11.84TYY253 pKa = 11.41SEE255 pKa = 4.21FVEE258 pKa = 5.52DD259 pKa = 4.07EE260 pKa = 3.95QSDD263 pKa = 3.54LSRR266 pKa = 11.84RR267 pKa = 11.84RR268 pKa = 11.84RR269 pKa = 11.84HH270 pKa = 5.2IDD272 pKa = 2.96ARR274 pKa = 11.84STLTKK279 pKa = 10.16KK280 pKa = 10.86GKK282 pKa = 9.79LL283 pKa = 3.5

Molecular weight: 33.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1382

83

524

276.4

31.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.525 ± 1.368

0.796 ± 0.435

6.223 ± 0.916

7.019 ± 1.326

3.907 ± 1.031

6.946 ± 0.501

1.809 ± 0.364

5.065 ± 0.357

5.355 ± 1.132

7.381 ± 0.659

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.256 ± 0.527

4.197 ± 0.412

4.92 ± 1.165

5.427 ± 1.224

7.742 ± 0.688

4.631 ± 0.518

6.006 ± 0.791

5.861 ± 0.857

1.737 ± 0.445

4.197 ± 0.541
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski