Amino acid dipepetide frequency for Pseudomonas phage Persinger

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.849AlaAla: 15.849 ± 1.545
1.358AlaCys: 1.358 ± 0.38
8.151AlaAsp: 8.151 ± 0.953
9.358AlaGlu: 9.358 ± 1.082
3.774AlaPhe: 3.774 ± 0.524
9.962AlaGly: 9.962 ± 1.124
2.038AlaHis: 2.038 ± 0.534
6.189AlaIle: 6.189 ± 0.545
5.962AlaLys: 5.962 ± 0.769
9.887AlaLeu: 9.887 ± 0.841
3.472AlaMet: 3.472 ± 0.503
4.075AlaAsn: 4.075 ± 0.597
4.075AlaPro: 4.075 ± 0.699
5.208AlaGln: 5.208 ± 0.699
8.0AlaArg: 8.0 ± 0.821
6.113AlaSer: 6.113 ± 0.649
7.321AlaThr: 7.321 ± 0.631
8.075AlaVal: 8.075 ± 0.893
2.264AlaTrp: 2.264 ± 0.386
3.094AlaTyr: 3.094 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
1.283CysAla: 1.283 ± 0.341
0.377CysCys: 0.377 ± 0.153
0.679CysAsp: 0.679 ± 0.271
1.132CysGlu: 1.132 ± 0.31
0.075CysPhe: 0.075 ± 0.069
1.057CysGly: 1.057 ± 0.348
0.302CysHis: 0.302 ± 0.146
0.377CysIle: 0.377 ± 0.18
0.604CysLys: 0.604 ± 0.263
0.302CysLeu: 0.302 ± 0.169
0.226CysMet: 0.226 ± 0.13
0.377CysAsn: 0.377 ± 0.222
0.906CysPro: 0.906 ± 0.238
0.226CysGln: 0.226 ± 0.117
1.132CysArg: 1.132 ± 0.296
0.453CysSer: 0.453 ± 0.167
0.453CysThr: 0.453 ± 0.171
0.981CysVal: 0.981 ± 0.273
0.226CysTrp: 0.226 ± 0.125
0.226CysTyr: 0.226 ± 0.129
0.0CysXaa: 0.0 ± 0.0
Asp
7.396AspAla: 7.396 ± 0.786
0.679AspCys: 0.679 ± 0.179
4.302AspAsp: 4.302 ± 0.694
4.0AspGlu: 4.0 ± 0.683
1.962AspPhe: 1.962 ± 0.45
7.245AspGly: 7.245 ± 0.662
0.981AspHis: 0.981 ± 0.319
1.887AspIle: 1.887 ± 0.404
2.415AspLys: 2.415 ± 0.404
5.736AspLeu: 5.736 ± 0.505
1.358AspMet: 1.358 ± 0.233
2.113AspAsn: 2.113 ± 0.347
3.019AspPro: 3.019 ± 0.414
3.245AspGln: 3.245 ± 0.447
3.321AspArg: 3.321 ± 0.506
1.811AspSer: 1.811 ± 0.358
2.491AspThr: 2.491 ± 0.477
4.755AspVal: 4.755 ± 0.673
1.057AspTrp: 1.057 ± 0.229
1.509AspTyr: 1.509 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
6.566GluAla: 6.566 ± 0.783
0.679GluCys: 0.679 ± 0.221
2.34GluAsp: 2.34 ± 0.487
3.321GluGlu: 3.321 ± 0.766
1.962GluPhe: 1.962 ± 0.411
4.83GluGly: 4.83 ± 0.642
1.283GluHis: 1.283 ± 0.322
3.849GluIle: 3.849 ± 0.58
2.792GluLys: 2.792 ± 0.621
7.396GluLeu: 7.396 ± 0.704
2.264GluMet: 2.264 ± 0.394
1.736GluAsn: 1.736 ± 0.303
1.962GluPro: 1.962 ± 0.43
3.396GluGln: 3.396 ± 0.539
4.604GluArg: 4.604 ± 0.676
2.491GluSer: 2.491 ± 0.511
2.642GluThr: 2.642 ± 0.379
4.0GluVal: 4.0 ± 0.541
1.736GluTrp: 1.736 ± 0.423
1.509GluTyr: 1.509 ± 0.371
0.0GluXaa: 0.0 ± 0.0
Phe
3.321PheAla: 3.321 ± 0.421
0.226PheCys: 0.226 ± 0.124
2.038PheAsp: 2.038 ± 0.366
1.811PheGlu: 1.811 ± 0.349
0.679PhePhe: 0.679 ± 0.249
2.792PheGly: 2.792 ± 0.417
0.302PheHis: 0.302 ± 0.136
1.509PheIle: 1.509 ± 0.282
1.887PheLys: 1.887 ± 0.393
1.962PheLeu: 1.962 ± 0.356
0.679PheMet: 0.679 ± 0.168
1.811PheAsn: 1.811 ± 0.351
0.906PhePro: 0.906 ± 0.231
1.283PheGln: 1.283 ± 0.3
1.811PheArg: 1.811 ± 0.296
1.358PheSer: 1.358 ± 0.283
2.038PheThr: 2.038 ± 0.533
1.736PheVal: 1.736 ± 0.351
0.453PheTrp: 0.453 ± 0.151
0.528PheTyr: 0.528 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
8.981GlyAla: 8.981 ± 1.163
0.83GlyCys: 0.83 ± 0.247
4.151GlyAsp: 4.151 ± 0.514
5.736GlyGlu: 5.736 ± 0.651
3.019GlyPhe: 3.019 ± 0.601
7.547GlyGly: 7.547 ± 0.919
1.736GlyHis: 1.736 ± 0.392
3.774GlyIle: 3.774 ± 0.477
5.811GlyLys: 5.811 ± 0.59
5.585GlyLeu: 5.585 ± 0.849
2.113GlyMet: 2.113 ± 0.405
2.792GlyAsn: 2.792 ± 0.46
2.415GlyPro: 2.415 ± 0.435
4.83GlyGln: 4.83 ± 0.562
5.509GlyArg: 5.509 ± 0.824
4.302GlySer: 4.302 ± 0.543
6.264GlyThr: 6.264 ± 0.769
6.868GlyVal: 6.868 ± 0.7
1.962GlyTrp: 1.962 ± 0.518
2.491GlyTyr: 2.491 ± 0.367
0.0GlyXaa: 0.0 ± 0.0
His
2.415HisAla: 2.415 ± 0.471
0.453HisCys: 0.453 ± 0.189
1.057HisAsp: 1.057 ± 0.239
1.132HisGlu: 1.132 ± 0.25
0.377HisPhe: 0.377 ± 0.177
1.887HisGly: 1.887 ± 0.273
0.151HisHis: 0.151 ± 0.105
0.528HisIle: 0.528 ± 0.188
0.679HisLys: 0.679 ± 0.236
2.264HisLeu: 2.264 ± 0.51
0.226HisMet: 0.226 ± 0.108
0.302HisAsn: 0.302 ± 0.144
0.528HisPro: 0.528 ± 0.197
0.83HisGln: 0.83 ± 0.216
1.132HisArg: 1.132 ± 0.256
0.981HisSer: 0.981 ± 0.229
0.83HisThr: 0.83 ± 0.229
0.453HisVal: 0.453 ± 0.171
0.377HisTrp: 0.377 ± 0.14
0.604HisTyr: 0.604 ± 0.248
0.0HisXaa: 0.0 ± 0.0
Ile
6.189IleAla: 6.189 ± 0.77
0.528IleCys: 0.528 ± 0.189
3.321IleAsp: 3.321 ± 0.433
2.868IleGlu: 2.868 ± 0.549
1.962IlePhe: 1.962 ± 0.449
4.755IleGly: 4.755 ± 0.828
0.453IleHis: 0.453 ± 0.246
2.113IleIle: 2.113 ± 0.427
2.113IleLys: 2.113 ± 0.38
2.415IleLeu: 2.415 ± 0.407
0.679IleMet: 0.679 ± 0.225
1.585IleAsn: 1.585 ± 0.396
1.962IlePro: 1.962 ± 0.284
1.887IleGln: 1.887 ± 0.453
2.717IleArg: 2.717 ± 0.402
2.264IleSer: 2.264 ± 0.38
2.34IleThr: 2.34 ± 0.438
2.868IleVal: 2.868 ± 0.485
0.528IleTrp: 0.528 ± 0.187
0.302IleTyr: 0.302 ± 0.146
0.0IleXaa: 0.0 ± 0.0
Lys
6.415LysAla: 6.415 ± 0.83
0.528LysCys: 0.528 ± 0.202
1.962LysAsp: 1.962 ± 0.382
1.66LysGlu: 1.66 ± 0.306
1.283LysPhe: 1.283 ± 0.264
4.0LysGly: 4.0 ± 0.737
0.906LysHis: 0.906 ± 0.258
1.585LysIle: 1.585 ± 0.306
1.509LysLys: 1.509 ± 0.303
3.849LysLeu: 3.849 ± 0.547
1.132LysMet: 1.132 ± 0.29
1.736LysAsn: 1.736 ± 0.272
3.396LysPro: 3.396 ± 0.708
2.189LysGln: 2.189 ± 0.422
3.849LysArg: 3.849 ± 0.511
2.34LysSer: 2.34 ± 0.377
2.34LysThr: 2.34 ± 0.329
3.094LysVal: 3.094 ± 0.613
0.604LysTrp: 0.604 ± 0.171
0.83LysTyr: 0.83 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
11.245LeuAla: 11.245 ± 1.229
0.679LeuCys: 0.679 ± 0.225
5.736LeuAsp: 5.736 ± 0.822
5.811LeuGlu: 5.811 ± 0.765
2.113LeuPhe: 2.113 ± 0.307
6.642LeuGly: 6.642 ± 0.733
1.283LeuHis: 1.283 ± 0.309
3.472LeuIle: 3.472 ± 0.502
2.792LeuLys: 2.792 ± 0.32
6.491LeuLeu: 6.491 ± 0.634
1.811LeuMet: 1.811 ± 0.404
2.717LeuAsn: 2.717 ± 0.413
3.925LeuPro: 3.925 ± 0.516
3.849LeuGln: 3.849 ± 0.553
7.019LeuArg: 7.019 ± 0.796
3.623LeuSer: 3.623 ± 0.576
5.434LeuThr: 5.434 ± 0.6
7.396LeuVal: 7.396 ± 0.507
0.981LeuTrp: 0.981 ± 0.25
1.283LeuTyr: 1.283 ± 0.349
0.0LeuXaa: 0.0 ± 0.0
Met
4.0MetAla: 4.0 ± 0.443
0.0MetCys: 0.0 ± 0.0
1.132MetAsp: 1.132 ± 0.296
0.906MetGlu: 0.906 ± 0.238
0.604MetPhe: 0.604 ± 0.198
1.585MetGly: 1.585 ± 0.32
0.453MetHis: 0.453 ± 0.183
0.981MetIle: 0.981 ± 0.278
1.208MetLys: 1.208 ± 0.362
2.264MetLeu: 2.264 ± 0.384
0.377MetMet: 0.377 ± 0.192
1.208MetAsn: 1.208 ± 0.277
1.283MetPro: 1.283 ± 0.289
1.057MetGln: 1.057 ± 0.262
2.113MetArg: 2.113 ± 0.414
1.132MetSer: 1.132 ± 0.29
1.509MetThr: 1.509 ± 0.313
1.736MetVal: 1.736 ± 0.458
0.075MetTrp: 0.075 ± 0.079
0.075MetTyr: 0.075 ± 0.07
0.0MetXaa: 0.0 ± 0.0
Asn
5.434AsnAla: 5.434 ± 0.546
0.528AsnCys: 0.528 ± 0.217
3.094AsnAsp: 3.094 ± 0.45
1.509AsnGlu: 1.509 ± 0.386
0.679AsnPhe: 0.679 ± 0.275
2.943AsnGly: 2.943 ± 0.402
0.604AsnHis: 0.604 ± 0.202
0.83AsnIle: 0.83 ± 0.223
1.358AsnLys: 1.358 ± 0.244
2.943AsnLeu: 2.943 ± 0.365
0.906AsnMet: 0.906 ± 0.225
1.434AsnAsn: 1.434 ± 0.33
2.038AsnPro: 2.038 ± 0.403
1.66AsnGln: 1.66 ± 0.382
2.113AsnArg: 2.113 ± 0.353
1.358AsnSer: 1.358 ± 0.342
1.66AsnThr: 1.66 ± 0.313
2.415AsnVal: 2.415 ± 0.38
0.604AsnTrp: 0.604 ± 0.172
0.679AsnTyr: 0.679 ± 0.188
0.0AsnXaa: 0.0 ± 0.0
Pro
6.491ProAla: 6.491 ± 0.689
0.528ProCys: 0.528 ± 0.195
3.396ProAsp: 3.396 ± 0.516
2.113ProGlu: 2.113 ± 0.377
1.434ProPhe: 1.434 ± 0.28
3.925ProGly: 3.925 ± 0.61
0.981ProHis: 0.981 ± 0.255
2.038ProIle: 2.038 ± 0.39
2.491ProLys: 2.491 ± 0.511
2.189ProLeu: 2.189 ± 0.336
1.283ProMet: 1.283 ± 0.337
1.434ProAsn: 1.434 ± 0.288
2.415ProPro: 2.415 ± 0.453
2.415ProGln: 2.415 ± 0.571
2.038ProArg: 2.038 ± 0.429
1.66ProSer: 1.66 ± 0.398
3.019ProThr: 3.019 ± 0.367
2.792ProVal: 2.792 ± 0.407
0.453ProTrp: 0.453 ± 0.213
1.057ProTyr: 1.057 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
5.736GlnAla: 5.736 ± 0.703
0.453GlnCys: 0.453 ± 0.186
2.113GlnAsp: 2.113 ± 0.415
2.868GlnGlu: 2.868 ± 0.439
1.434GlnPhe: 1.434 ± 0.345
3.547GlnGly: 3.547 ± 0.599
1.132GlnHis: 1.132 ± 0.292
1.434GlnIle: 1.434 ± 0.289
1.283GlnLys: 1.283 ± 0.22
4.906GlnLeu: 4.906 ± 0.672
1.283GlnMet: 1.283 ± 0.351
0.981GlnAsn: 0.981 ± 0.197
2.264GlnPro: 2.264 ± 0.335
3.17GlnGln: 3.17 ± 0.9
3.925GlnArg: 3.925 ± 0.708
2.566GlnSer: 2.566 ± 0.518
2.868GlnThr: 2.868 ± 0.386
3.094GlnVal: 3.094 ± 0.58
0.906GlnTrp: 0.906 ± 0.268
1.132GlnTyr: 1.132 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
8.151ArgAla: 8.151 ± 0.762
0.83ArgCys: 0.83 ± 0.277
4.528ArgAsp: 4.528 ± 0.672
5.66ArgGlu: 5.66 ± 0.786
1.736ArgPhe: 1.736 ± 0.33
5.887ArgGly: 5.887 ± 0.594
1.132ArgHis: 1.132 ± 0.271
2.491ArgIle: 2.491 ± 0.356
3.245ArgLys: 3.245 ± 0.692
6.264ArgLeu: 6.264 ± 0.6
1.736ArgMet: 1.736 ± 0.384
2.264ArgAsn: 2.264 ± 0.36
3.019ArgPro: 3.019 ± 0.596
3.547ArgGln: 3.547 ± 0.68
6.491ArgArg: 6.491 ± 0.765
2.868ArgSer: 2.868 ± 0.497
3.623ArgThr: 3.623 ± 0.478
4.679ArgVal: 4.679 ± 0.515
1.057ArgTrp: 1.057 ± 0.301
1.358ArgTyr: 1.358 ± 0.364
0.0ArgXaa: 0.0 ± 0.0
Ser
5.057SerAla: 5.057 ± 0.544
0.604SerCys: 0.604 ± 0.21
3.094SerAsp: 3.094 ± 0.475
2.491SerGlu: 2.491 ± 0.491
1.585SerPhe: 1.585 ± 0.382
3.925SerGly: 3.925 ± 0.691
0.755SerHis: 0.755 ± 0.239
3.396SerIle: 3.396 ± 0.48
3.094SerLys: 3.094 ± 0.394
3.698SerLeu: 3.698 ± 0.525
0.755SerMet: 0.755 ± 0.235
1.283SerAsn: 1.283 ± 0.312
1.585SerPro: 1.585 ± 0.369
1.585SerGln: 1.585 ± 0.364
2.717SerArg: 2.717 ± 0.386
1.887SerSer: 1.887 ± 0.293
2.264SerThr: 2.264 ± 0.425
4.0SerVal: 4.0 ± 0.545
0.981SerTrp: 0.981 ± 0.412
0.679SerTyr: 0.679 ± 0.28
0.0SerXaa: 0.0 ± 0.0
Thr
7.774ThrAla: 7.774 ± 0.942
0.302ThrCys: 0.302 ± 0.136
3.019ThrAsp: 3.019 ± 0.415
2.792ThrGlu: 2.792 ± 0.445
2.264ThrPhe: 2.264 ± 0.412
5.434ThrGly: 5.434 ± 0.591
0.528ThrHis: 0.528 ± 0.228
3.396ThrIle: 3.396 ± 0.413
1.811ThrLys: 1.811 ± 0.312
5.736ThrLeu: 5.736 ± 0.763
1.208ThrMet: 1.208 ± 0.33
2.113ThrAsn: 2.113 ± 0.587
4.0ThrPro: 4.0 ± 0.507
1.887ThrGln: 1.887 ± 0.362
2.566ThrArg: 2.566 ± 0.425
2.491ThrSer: 2.491 ± 0.38
4.755ThrThr: 4.755 ± 0.828
4.453ThrVal: 4.453 ± 0.65
1.057ThrTrp: 1.057 ± 0.245
1.358ThrTyr: 1.358 ± 0.301
0.0ThrXaa: 0.0 ± 0.0
Val
7.698ValAla: 7.698 ± 0.837
0.906ValCys: 0.906 ± 0.302
4.755ValAsp: 4.755 ± 0.673
4.226ValGlu: 4.226 ± 0.551
1.358ValPhe: 1.358 ± 0.295
5.358ValGly: 5.358 ± 0.74
1.283ValHis: 1.283 ± 0.344
3.17ValIle: 3.17 ± 0.594
2.642ValLys: 2.642 ± 0.424
6.566ValLeu: 6.566 ± 0.637
1.434ValMet: 1.434 ± 0.345
2.792ValAsn: 2.792 ± 0.454
3.094ValPro: 3.094 ± 0.504
3.094ValGln: 3.094 ± 0.459
5.962ValArg: 5.962 ± 0.67
4.151ValSer: 4.151 ± 0.592
5.283ValThr: 5.283 ± 0.744
5.509ValVal: 5.509 ± 0.727
0.906ValTrp: 0.906 ± 0.286
1.585ValTyr: 1.585 ± 0.37
0.0ValXaa: 0.0 ± 0.0
Trp
1.434TrpAla: 1.434 ± 0.386
0.226TrpCys: 0.226 ± 0.11
0.906TrpAsp: 0.906 ± 0.229
1.132TrpGlu: 1.132 ± 0.271
0.377TrpPhe: 0.377 ± 0.169
1.509TrpGly: 1.509 ± 0.415
0.528TrpHis: 0.528 ± 0.176
0.226TrpIle: 0.226 ± 0.102
0.981TrpLys: 0.981 ± 0.274
1.66TrpLeu: 1.66 ± 0.387
0.453TrpMet: 0.453 ± 0.191
0.981TrpAsn: 0.981 ± 0.266
0.453TrpPro: 0.453 ± 0.215
0.981TrpGln: 0.981 ± 0.35
1.358TrpArg: 1.358 ± 0.305
0.83TrpSer: 0.83 ± 0.307
1.057TrpThr: 1.057 ± 0.35
1.358TrpVal: 1.358 ± 0.295
0.604TrpTrp: 0.604 ± 0.203
0.377TrpTyr: 0.377 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.792TyrAla: 2.792 ± 0.389
0.679TyrCys: 0.679 ± 0.224
1.509TyrAsp: 1.509 ± 0.332
0.83TyrGlu: 0.83 ± 0.267
0.453TyrPhe: 0.453 ± 0.153
1.887TyrGly: 1.887 ± 0.426
0.377TyrHis: 0.377 ± 0.173
0.604TyrIle: 0.604 ± 0.244
0.528TyrLys: 0.528 ± 0.166
2.189TyrLeu: 2.189 ± 0.434
0.226TyrMet: 0.226 ± 0.112
1.057TyrAsn: 1.057 ± 0.28
0.981TyrPro: 0.981 ± 0.26
0.755TyrGln: 0.755 ± 0.249
2.189TyrArg: 2.189 ± 0.447
0.755TyrSer: 0.755 ± 0.189
0.755TyrThr: 0.755 ± 0.276
1.509TyrVal: 1.509 ± 0.37
0.604TyrTrp: 0.604 ± 0.188
0.755TyrTyr: 0.755 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (13251 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski