Enterobacteria phage 9g

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Queuovirinae; Nonagvirus; Escherichia virus 9g

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KU98|X2KU98_9CAUD Uncharacterized protein OS=Enterobacteria phage 9g OX=1468411 GN=9g_00049 PE=4 SV=1
MM1 pKa = 6.79TQDD4 pKa = 3.39LVVYY8 pKa = 8.5PIYY11 pKa = 11.0GDD13 pKa = 3.78GKK15 pKa = 10.91LGLPDD20 pKa = 3.5TNEE23 pKa = 4.41FIAEE27 pKa = 4.45GSAFEE32 pKa = 4.35FLMGAMAGGCTAEE45 pKa = 4.04EE46 pKa = 4.35AVRR49 pKa = 11.84LACVYY54 pKa = 10.51CDD56 pKa = 3.32SCGQGVKK63 pKa = 10.27FYY65 pKa = 11.35DD66 pKa = 3.07VAYY69 pKa = 7.94TLKK72 pKa = 10.58VAQCKK77 pKa = 9.89IEE79 pKa = 4.11EE80 pKa = 4.32KK81 pKa = 10.72PFF83 pKa = 4.28

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2L044|X2L044_9CAUD Uncharacterized protein OS=Enterobacteria phage 9g OX=1468411 GN=9g_00048 PE=4 SV=1
MM1 pKa = 7.58SLQRR5 pKa = 11.84FDD7 pKa = 3.91NAEE10 pKa = 4.08LACEE14 pKa = 3.76FAKK17 pKa = 10.61RR18 pKa = 11.84KK19 pKa = 8.18FAKK22 pKa = 9.66TGTPRR27 pKa = 11.84CVHH30 pKa = 6.41AAIGQNGAKK39 pKa = 8.49YY40 pKa = 8.9WYY42 pKa = 9.12VGTVAQIKK50 pKa = 10.12RR51 pKa = 11.84RR52 pKa = 11.84FATTPEE58 pKa = 3.82MRR60 pKa = 11.84RR61 pKa = 11.84LGSPTLMRR69 pKa = 11.84NRR71 pKa = 11.84QLDD74 pKa = 3.52KK75 pKa = 11.47EE76 pKa = 4.37EE77 pKa = 4.38IYY79 pKa = 10.95QFLRR83 pKa = 11.84NYY85 pKa = 8.23TGKK88 pKa = 10.43EE89 pKa = 3.41NRR91 pKa = 11.84FQVAAKK97 pKa = 9.03EE98 pKa = 4.11FNIGLSTAYY107 pKa = 9.54KK108 pKa = 9.21IYY110 pKa = 10.65HH111 pKa = 5.6MYY113 pKa = 10.64GRR115 pKa = 11.84LII117 pKa = 3.74

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

16973

24

1211

239.1

26.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.514 ± 0.741

1.361 ± 0.17

5.809 ± 0.198

6.622 ± 0.293

3.506 ± 0.212

7.129 ± 0.27

1.95 ± 0.144

6.08 ± 0.257

6.015 ± 0.244

7.353 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.893 ± 0.18

4.878 ± 0.205

3.871 ± 0.24

4.654 ± 0.456

5.232 ± 0.253

6.08 ± 0.3

6.151 ± 0.311

6.905 ± 0.269

1.426 ± 0.133

3.57 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski