Cathartes aura (Turkey vulture) (Vultur aura)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091L3X3|A0A091L3X3_CATAU Matrix metalloproteinase-15 (Fragment) OS=Cathartes aura OX=43455 GN=N323_06363 PE=3 SV=1
SS1 pKa = 6.72 RR2 pKa = 11.84 LSWLEE7 pKa = 3.51 NSGVGFSLDD16 pKa = 3.46 YY17 pKa = 9.24 PTISLHH23 pKa = 6.36 AVSRR27 pKa = 11.84 DD28 pKa = 3.07 LNAYY32 pKa = 7.9 PWEE35 pKa = 4.19 HH36 pKa = 6.86 LYY38 pKa = 11.74 VMVNAKK44 pKa = 10.31 FEE46 pKa = 4.15 EE47 pKa = 4.78 EE48 pKa = 4.13 EE49 pKa = 4.25 TKK51 pKa = 10.06 EE52 pKa = 3.96 APMAEE57 pKa = 4.2 GEE59 pKa = 4.24 EE60 pKa = 4.28 EE61 pKa = 4.66 EE62 pKa = 4.91 EE63 pKa = 5.45 DD64 pKa = 3.82 SDD66 pKa = 6.17 DD67 pKa = 3.89 DD68 pKa = 4.15 VEE70 pKa = 5.15 PIAEE74 pKa = 4.09 FRR76 pKa = 11.84 FVPSDD81 pKa = 3.36 KK82 pKa = 10.88 SALEE86 pKa = 3.9 AMFSAMCEE94 pKa = 4.27 CQALHH99 pKa = 7.41 PDD101 pKa = 4.21 PDD103 pKa = 5.77 DD104 pKa = 4.05 EE105 pKa = 7.61 DD106 pKa = 4.46 SDD108 pKa = 3.76 NDD110 pKa = 3.97 YY111 pKa = 11.37 EE112 pKa = 4.59 GEE114 pKa = 4.16 EE115 pKa = 4.41 YY116 pKa = 10.56 DD117 pKa = 4.4 VEE119 pKa = 4.23 AHH121 pKa = 6.13 EE122 pKa = 5.43 LEE124 pKa = 4.54 QGDD127 pKa = 3.63 IPTFYY132 pKa = 10.0 TYY134 pKa = 11.09 EE135 pKa = 4.17 EE136 pKa = 4.62 GLSHH140 pKa = 6.9 LTAEE144 pKa = 4.71 GQATLEE150 pKa = 4.11 RR151 pKa = 11.84 LEE153 pKa = 4.74 GMLAQSVSSQYY164 pKa = 11.66 NMAGVRR170 pKa = 11.84 TEE172 pKa = 3.7 DD173 pKa = 3.58 SIRR176 pKa = 11.84 EE177 pKa = 4.1 FEE179 pKa = 5.37 DD180 pKa = 3.48 GMEE183 pKa = 3.92 VDD185 pKa = 3.78 IAPVVAGQFEE195 pKa = 4.42 DD196 pKa = 5.26 AEE198 pKa = 4.32 VDD200 pKa = 3.59 HH201 pKa = 7.08
Molecular weight: 22.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.76
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.567
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.592
Grimsley 3.478
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 3.973
Thurlkill 3.592
EMBOSS 3.617
Sillero 3.872
Patrickios 1.825
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A091LBA1|A0A091LBA1_CATAU Perilipin-1 (Fragment) OS=Cathartes aura OX=43455 GN=N323_03660 PE=3 SV=1
PP1 pKa = 6.86 TAPSQEE7 pKa = 4.06 RR8 pKa = 11.84 ARR10 pKa = 11.84 QGKK13 pKa = 9.5 AGQGKK18 pKa = 9.33 ARR20 pKa = 11.84 QGKK23 pKa = 8.73 ARR25 pKa = 11.84 QGKK28 pKa = 8.73 ARR30 pKa = 11.84 QGKK33 pKa = 8.73 ARR35 pKa = 11.84 QGKK38 pKa = 8.7 ARR40 pKa = 11.84 QGRR43 pKa = 11.84 ARR45 pKa = 11.84 QGKK48 pKa = 8.92 ARR50 pKa = 11.84 RR51 pKa = 11.84 AKK53 pKa = 10.35
Molecular weight: 5.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.427
IPC2_protein 10.921
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.07
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7760
0
7760
3012677
31
4590
388.2
43.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.304 ± 0.025
2.29 ± 0.027
5.035 ± 0.019
7.096 ± 0.031
3.987 ± 0.021
5.78 ± 0.031
2.531 ± 0.016
5.144 ± 0.024
6.477 ± 0.029
9.675 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.265 ± 0.01
4.262 ± 0.02
5.206 ± 0.032
4.623 ± 0.026
5.131 ± 0.022
8.193 ± 0.04
5.444 ± 0.02
6.306 ± 0.023
1.198 ± 0.01
3.042 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here