Vanilla virus X
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220NQ60|A0A220NQ60_9VIRU Triple gene block protein 2 OS=Vanilla virus X OX=2016427 GN=TGBp2 PE=4 SV=1
MM1 pKa = 7.43 ANIRR5 pKa = 11.84 AALDD9 pKa = 3.48 RR10 pKa = 11.84 FTDD13 pKa = 3.67 TSIKK17 pKa = 10.51 SALQEE22 pKa = 3.76 QAYY25 pKa = 10.87 SDD27 pKa = 3.84 VKK29 pKa = 10.98 PEE31 pKa = 3.69 LRR33 pKa = 11.84 AINSLNPYY41 pKa = 8.97 AQSTSGADD49 pKa = 3.5 DD50 pKa = 4.44 LEE52 pKa = 4.16 QLGIGTNPFSIKK64 pKa = 10.4 LHH66 pKa = 4.52 THH68 pKa = 6.18 AAAKK72 pKa = 10.11 AVEE75 pKa = 3.89 NRR77 pKa = 11.84 MLEE80 pKa = 4.18 IVGHH84 pKa = 5.71 HH85 pKa = 6.93 LPRR88 pKa = 11.84 DD89 pKa = 3.38 SCTFLWLKK97 pKa = 9.87 KK98 pKa = 10.59 AKK100 pKa = 10.54 LNLLRR105 pKa = 11.84 RR106 pKa = 11.84 NNPLDD111 pKa = 3.26 TFRR114 pKa = 11.84 NVPFEE119 pKa = 4.49 PKK121 pKa = 10.3 DD122 pKa = 3.25 FARR125 pKa = 11.84 YY126 pKa = 9.0 EE127 pKa = 4.14 EE128 pKa = 4.23 EE129 pKa = 4.61 TICSSINGITDD140 pKa = 3.14 ATVYY144 pKa = 10.59 ISDD147 pKa = 4.46 ALHH150 pKa = 5.92 FMKK153 pKa = 10.52 PSFVLGLFAGNPSIQQVYY171 pKa = 7.99 ATLVLPPEE179 pKa = 4.73 ALHH182 pKa = 5.95 KK183 pKa = 10.62 HH184 pKa = 6.0 PSAHH188 pKa = 6.39 PNLYY192 pKa = 10.03 NINYY196 pKa = 9.25 NFGGFQYY203 pKa = 10.57 IPGKK207 pKa = 9.59 HH208 pKa = 6.09 AGGAYY213 pKa = 9.41 HH214 pKa = 7.22 HH215 pKa = 6.47 EE216 pKa = 4.51 LEE218 pKa = 4.26 TLEE221 pKa = 3.92 WLKK224 pKa = 10.63 IGKK227 pKa = 8.86 IFYY230 pKa = 10.8 NKK232 pKa = 10.26 GLTDD236 pKa = 3.33 QKK238 pKa = 11.32 VITVQLLEE246 pKa = 4.25 SLGANHH252 pKa = 7.35 LFLFRR257 pKa = 11.84 RR258 pKa = 11.84 GDD260 pKa = 3.19 WVTPRR265 pKa = 11.84 VRR267 pKa = 11.84 TFSLDD272 pKa = 3.34 EE273 pKa = 4.32 YY274 pKa = 9.96 VTLPQIFHH282 pKa = 7.32 PKK284 pKa = 8.85 LANASRR290 pKa = 11.84 PIRR293 pKa = 11.84 FTLANQLLLYY303 pKa = 10.05 VKK305 pKa = 10.03 SIKK308 pKa = 9.6 TATQRR313 pKa = 11.84 DD314 pKa = 3.72 IFAKK318 pKa = 10.2 LRR320 pKa = 11.84 QLIKK324 pKa = 10.18 TEE326 pKa = 4.08 DD327 pKa = 3.59 LSRR330 pKa = 11.84 YY331 pKa = 10.0 APDD334 pKa = 3.59 EE335 pKa = 4.0 LVHH338 pKa = 6.15 IANYY342 pKa = 9.98 FLFVGEE348 pKa = 5.09 LSAEE352 pKa = 3.92 NCYY355 pKa = 11.44 DD356 pKa = 3.61 NLLKK360 pKa = 10.4 MGFFRR365 pKa = 11.84 KK366 pKa = 8.49 CTVPIKK372 pKa = 10.75 NAFLKK377 pKa = 10.47 LWRR380 pKa = 11.84 TLFGKK385 pKa = 10.8 LEE387 pKa = 4.0 FEE389 pKa = 4.46 QLLQSLEE396 pKa = 4.24 WKK398 pKa = 9.41 PFSYY402 pKa = 10.89 SLLVIDD408 pKa = 5.69 RR409 pKa = 11.84 EE410 pKa = 4.46 YY411 pKa = 11.21 NDD413 pKa = 3.05 YY414 pKa = 9.37 TVRR417 pKa = 11.84 VEE419 pKa = 3.86 ARR421 pKa = 11.84 KK422 pKa = 9.53 EE423 pKa = 3.95 KK424 pKa = 9.54 YY425 pKa = 9.8 HH426 pKa = 6.43 KK427 pKa = 10.69 LNSQFHH433 pKa = 5.1 STWAIEE439 pKa = 4.08 DD440 pKa = 3.99 TEE442 pKa = 5.07 ADD444 pKa = 3.02 IDD446 pKa = 4.18 DD447 pKa = 4.67 LVTRR451 pKa = 11.84 MTTEE455 pKa = 3.47 LHH457 pKa = 6.39 IPLPLPSPNDD467 pKa = 3.69 DD468 pKa = 3.63 GQKK471 pKa = 10.84 GNSEE475 pKa = 4.02 EE476 pKa = 5.55 AKK478 pKa = 8.45 TQPEE482 pKa = 4.4 APMPAAEE489 pKa = 5.27 KK490 pKa = 10.93 GPTDD494 pKa = 3.62 DD495 pKa = 3.96 TSSPFAGGEE504 pKa = 4.08 EE505 pKa = 4.4 EE506 pKa = 4.61 PSNIADD512 pKa = 3.28 DD513 pKa = 4.11 HH514 pKa = 6.21 EE515 pKa = 4.76 RR516 pKa = 11.84 VNSSCEE522 pKa = 3.62 ASTSQNAPAHH532 pKa = 6.38 PGTSKK537 pKa = 10.62 EE538 pKa = 3.91 RR539 pKa = 11.84 TVRR542 pKa = 11.84 NAYY545 pKa = 9.57 FLHH548 pKa = 6.57 AMRR551 pKa = 11.84 KK552 pKa = 8.63 AASDD556 pKa = 3.83 PNVFTDD562 pKa = 3.82 INDD565 pKa = 4.88 AILGACEE572 pKa = 3.46 KK573 pKa = 9.68 MEE575 pKa = 4.59 RR576 pKa = 11.84 SGCVDD581 pKa = 3.74 SPWSPWASILNACGFSGDD599 pKa = 4.44 YY600 pKa = 9.9 IQHH603 pKa = 5.78 DD604 pKa = 3.42 QRR606 pKa = 11.84 YY607 pKa = 8.2 NLIYY611 pKa = 9.8 PIQDD615 pKa = 2.56 IKK617 pKa = 11.35 AVNQCPWPITVPTRR631 pKa = 11.84 LAAALEE637 pKa = 4.3 KK638 pKa = 9.83 MGRR641 pKa = 11.84 PATHH645 pKa = 6.77 AKK647 pKa = 9.81 IEE649 pKa = 4.19 SRR651 pKa = 11.84 RR652 pKa = 11.84 AAAFASDD659 pKa = 3.98 VKK661 pKa = 10.99 NSRR664 pKa = 11.84 TGMLLPKK671 pKa = 10.33 QPIDD675 pKa = 3.13 WKK677 pKa = 10.66 QAFASKK683 pKa = 10.71 CDD685 pKa = 3.67 FADD688 pKa = 3.59 TKK690 pKa = 11.13 NFPCVVIHH698 pKa = 6.43 GAGGSGKK705 pKa = 10.18 SRR707 pKa = 11.84 LIQNFLKK714 pKa = 10.1 TVDD717 pKa = 2.99 RR718 pKa = 11.84 RR719 pKa = 11.84 EE720 pKa = 3.77 RR721 pKa = 11.84 RR722 pKa = 11.84 FCVVTPTLEE731 pKa = 5.14 LRR733 pKa = 11.84 NDD735 pKa = 3.43 WLKK738 pKa = 10.86 KK739 pKa = 10.02 VPQLPLEE746 pKa = 4.2 VFKK749 pKa = 10.7 TFEE752 pKa = 3.86 KK753 pKa = 11.12 AMVQGSASIVIMDD766 pKa = 5.78 DD767 pKa = 3.55 YY768 pKa = 11.37 TKK770 pKa = 11.07 LPAGYY775 pKa = 8.87 IEE777 pKa = 4.9 AYY779 pKa = 9.76 IMQHH783 pKa = 6.28 PNVDD787 pKa = 4.22 LLILTGDD794 pKa = 4.29 PKK796 pKa = 10.75 QSSHH800 pKa = 7.13 HH801 pKa = 5.72 EE802 pKa = 4.18 QNSQAQISHH811 pKa = 7.34 LEE813 pKa = 3.82 PAHH816 pKa = 6.2 TIFGQYY822 pKa = 9.31 AKK824 pKa = 10.66 YY825 pKa = 10.26 YY826 pKa = 10.33 INATHH831 pKa = 6.97 RR832 pKa = 11.84 NCSEE836 pKa = 3.83 LANPLGVYY844 pKa = 9.89 SEE846 pKa = 4.49 NFLPLKK852 pKa = 10.24 LALSSQVSDD861 pKa = 4.16 GPILVPSQLKK871 pKa = 10.51 SEE873 pKa = 4.43 ALHH876 pKa = 5.26 EE877 pKa = 4.76 LGRR880 pKa = 11.84 KK881 pKa = 8.81 AYY883 pKa = 9.15 TYY885 pKa = 10.73 AGCQGLTTPSIQIMLDD901 pKa = 3.22 QNTPLCSNEE910 pKa = 4.16 VIYY913 pKa = 9.14 TALSRR918 pKa = 11.84 AVDD921 pKa = 4.52 EE922 pKa = 4.22 ITFINTGSNSTDD934 pKa = 3.51 FWRR937 pKa = 11.84 KK938 pKa = 8.26 MDD940 pKa = 3.63 CTPYY944 pKa = 11.05 LKK946 pKa = 9.89 TFLQAVRR953 pKa = 11.84 EE954 pKa = 4.18 PKK956 pKa = 10.67 LPTEE960 pKa = 4.27 KK961 pKa = 11.03 CEE963 pKa = 4.17 DD964 pKa = 3.73 PQPRR968 pKa = 11.84 EE969 pKa = 4.23 PIIKK973 pKa = 8.3 THH975 pKa = 6.53 FPVEE979 pKa = 3.97 NEE981 pKa = 3.67 KK982 pKa = 11.26 VIFEE986 pKa = 4.29 KK987 pKa = 10.57 EE988 pKa = 3.57 ISEE991 pKa = 4.19 LQDD994 pKa = 2.93 KK995 pKa = 10.09 FSRR998 pKa = 11.84 EE999 pKa = 4.04 LHH1001 pKa = 5.73 TPAHH1005 pKa = 5.51 GHH1007 pKa = 5.77 SNAIQTEE1014 pKa = 4.19 DD1015 pKa = 3.83 TIVQLFQHH1023 pKa = 5.59 QQAKK1027 pKa = 10.42 DD1028 pKa = 3.36 QTLYY1032 pKa = 10.05 WATIDD1037 pKa = 4.94 KK1038 pKa = 10.24 RR1039 pKa = 11.84 IKK1041 pKa = 10.56 LATEE1045 pKa = 3.61 EE1046 pKa = 4.52 ANLKK1050 pKa = 10.42 EE1051 pKa = 4.28 FVFKK1055 pKa = 10.94 KK1056 pKa = 10.65 DD1057 pKa = 2.8 IGDD1060 pKa = 3.78 ILFLNYY1066 pKa = 9.51 KK1067 pKa = 9.85 EE1068 pKa = 5.27 AMNLPNQPIPFDD1080 pKa = 3.5 LQLYY1084 pKa = 6.82 EE1085 pKa = 4.06 NCKK1088 pKa = 10.54 SEE1090 pKa = 4.33 VQNTYY1095 pKa = 9.92 LKK1097 pKa = 10.8 KK1098 pKa = 10.48 PISQLMNGEE1107 pKa = 4.55 LRR1109 pKa = 11.84 QSPDD1113 pKa = 3.29 FHH1115 pKa = 6.66 PHH1117 pKa = 7.33 AIQLFLKK1124 pKa = 9.31 SQWVKK1129 pKa = 11.17 KK1130 pKa = 9.94 NDD1132 pKa = 4.39 CIGLLQVKK1140 pKa = 9.75 AGQTIASFMQQTVMIFGTMARR1161 pKa = 11.84 YY1162 pKa = 7.4 MRR1164 pKa = 11.84 RR1165 pKa = 11.84 MRR1167 pKa = 11.84 QRR1169 pKa = 11.84 YY1170 pKa = 5.77 QPRR1173 pKa = 11.84 KK1174 pKa = 9.91 IMINCEE1180 pKa = 4.05 TTPEE1184 pKa = 4.94 DD1185 pKa = 2.88 ISKK1188 pKa = 10.23 FLKK1191 pKa = 10.49 QEE1193 pKa = 3.94 WNFKK1197 pKa = 10.12 RR1198 pKa = 11.84 PAYY1201 pKa = 10.49 ANDD1204 pKa = 3.24 YY1205 pKa = 10.91 SAFDD1209 pKa = 3.64 QSQDD1213 pKa = 2.94 GAMLQFEE1220 pKa = 4.74 VIKK1223 pKa = 10.67 AKK1225 pKa = 8.22 YY1226 pKa = 8.65 HH1227 pKa = 6.39 CIPEE1231 pKa = 4.4 EE1232 pKa = 5.13 IIDD1235 pKa = 3.85 AYY1237 pKa = 10.06 IAIKK1241 pKa = 10.66 LNAKK1245 pKa = 9.71 IFLGTLAIMRR1255 pKa = 11.84 LTGEE1259 pKa = 4.65 GPTFDD1264 pKa = 6.01 ANTEE1268 pKa = 4.14 CNIAYY1273 pKa = 9.91 HH1274 pKa = 5.83 FARR1277 pKa = 11.84 FNMPPDD1283 pKa = 3.44 TAYY1286 pKa = 11.19 LFAGDD1291 pKa = 5.95 DD1292 pKa = 3.84 MAQDD1296 pKa = 3.59 APVEE1300 pKa = 4.39 EE1301 pKa = 4.67 KK1302 pKa = 11.12 SSFAKK1307 pKa = 10.41 IKK1309 pKa = 10.64 DD1310 pKa = 3.74 RR1311 pKa = 11.84 LSLTSKK1317 pKa = 10.48 PLTFKK1322 pKa = 10.7 QGPGDD1327 pKa = 3.61 YY1328 pKa = 10.99 ASFCGSLITPLGLLKK1343 pKa = 10.24 EE1344 pKa = 4.18 ARR1346 pKa = 11.84 KK1347 pKa = 9.02 LAASLEE1353 pKa = 4.23 LANRR1357 pKa = 11.84 TGNLKK1362 pKa = 10.11 NVAASYY1368 pKa = 10.47 AQDD1371 pKa = 3.17 IFPTFVQGDD1380 pKa = 4.45 RR1381 pKa = 11.84 IYY1383 pKa = 10.86 DD1384 pKa = 3.21 IFTPEE1389 pKa = 4.06 EE1390 pKa = 3.99 QTHH1393 pKa = 5.96 HH1394 pKa = 6.46 RR1395 pKa = 11.84 NSVRR1399 pKa = 11.84 FLVKK1403 pKa = 10.11 SAHH1406 pKa = 5.01 QNFGEE1411 pKa = 4.6 LVAQKK1416 pKa = 10.64 LAGDD1420 pKa = 4.14 SDD1422 pKa = 4.7 SDD1424 pKa = 5.15 DD1425 pKa = 4.14 EE1426 pKa = 4.47 DD1427 pKa = 3.96 TGDD1430 pKa = 4.26 SLMKK1434 pKa = 10.44 HH1435 pKa = 6.35 LSS1437 pKa = 3.28
Molecular weight: 162.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.15
IPC2_protein 6.122
IPC_protein 6.198
Toseland 6.532
ProMoST 6.59
Dawson 6.532
Bjellqvist 6.532
Wikipedia 6.547
Rodwell 6.532
Grimsley 6.678
Solomon 6.532
Lehninger 6.532
Nozaki 6.825
DTASelect 6.985
Thurlkill 7.015
EMBOSS 6.985
Sillero 6.927
Patrickios 4.24
IPC_peptide 6.547
IPC2_peptide 6.781
IPC2.peptide.svr19 6.78
Protein with the highest isoelectric point:
>tr|A0A220NQ63|A0A220NQ63_9VIRU Movement protein TGBp3 OS=Vanilla virus X OX=2016427 GN=TGBp3 PE=3 SV=1
MM1 pKa = 7.6 PLIHH5 pKa = 7.15 PPDD8 pKa = 3.6 HH9 pKa = 6.51 TNAIRR14 pKa = 11.84 IAAVFLGVTLTIYY27 pKa = 11.01 ALRR30 pKa = 11.84 VNTLPHH36 pKa = 6.52 VGDD39 pKa = 4.69 NIHH42 pKa = 6.79 ALPHH46 pKa = 5.85 GGRR49 pKa = 11.84 YY50 pKa = 8.24 QDD52 pKa = 3.33 GTKK55 pKa = 10.07 TIFYY59 pKa = 9.27 NSPFRR64 pKa = 11.84 SQVPSTLPAILALILPAIIYY84 pKa = 9.86 ALSRR88 pKa = 11.84 HH89 pKa = 5.39 HH90 pKa = 6.48 RR91 pKa = 11.84 TRR93 pKa = 11.84 VVTAWADD100 pKa = 3.59 THH102 pKa = 6.39 HH103 pKa = 6.72 HH104 pKa = 5.93 HH105 pKa = 6.78 HH106 pKa = 6.9 HH107 pKa = 6.66 PAA109 pKa = 3.89
Molecular weight: 12.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.026
IPC2_protein 9.853
IPC_protein 10.672
Toseland 9.955
ProMoST 9.999
Dawson 10.306
Bjellqvist 10.145
Wikipedia 10.613
Rodwell 10.306
Grimsley 10.452
Solomon 10.394
Lehninger 10.335
Nozaki 9.911
DTASelect 10.145
Thurlkill 10.101
EMBOSS 10.409
Sillero 10.218
Patrickios 7.571
IPC_peptide 10.365
IPC2_peptide 9.209
IPC2.peptide.svr19 8.333
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2052
62
1437
410.4
45.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.064 ± 1.373
1.559 ± 0.194
5.458 ± 0.491
5.068 ± 1.467
4.581 ± 0.702
4.873 ± 0.424
3.46 ± 1.25
6.823 ± 1.092
5.653 ± 1.333
9.698 ± 1.147
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.657 ± 0.351
4.63 ± 0.559
6.287 ± 0.693
4.678 ± 0.516
4.678 ± 0.322
5.75 ± 0.637
7.115 ± 1.384
4.922 ± 1.021
0.975 ± 0.141
3.07 ± 0.665
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here