Ruminobacter amylophilus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales; Succinivibrionaceae; Ruminobacter

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A662ZL84|A0A662ZL84_9GAMM Uncharacterized protein OS=Ruminobacter amylophilus OX=867 GN=SAMN02910344_02349 PE=4 SV=1
MM1 pKa = 7.29SLDD4 pKa = 3.66YY5 pKa = 10.76EE6 pKa = 4.44DD7 pKa = 5.56RR8 pKa = 11.84GPLSAIKK15 pKa = 10.28DD16 pKa = 3.66YY17 pKa = 11.81AKK19 pKa = 10.94FNDD22 pKa = 4.0LDD24 pKa = 3.93NFYY27 pKa = 11.2HH28 pKa = 5.66MTDD31 pKa = 3.49QIVGEE36 pKa = 4.26ILADD40 pKa = 3.74GNPEE44 pKa = 3.9DD45 pKa = 4.06EE46 pKa = 5.0EE47 pKa = 4.68YY48 pKa = 10.99DD49 pKa = 3.54IIHH52 pKa = 6.23YY53 pKa = 9.75FSSLNFANLEE63 pKa = 4.11KK64 pKa = 10.67FVVEE68 pKa = 4.15VNNVFGDD75 pKa = 3.96EE76 pKa = 4.6IYY78 pKa = 10.4IEE80 pKa = 5.38DD81 pKa = 4.4PDD83 pKa = 3.67EE84 pKa = 5.36CFDD87 pKa = 3.88EE88 pKa = 4.99EE89 pKa = 4.39YY90 pKa = 10.88KK91 pKa = 10.04EE92 pKa = 4.47TFFSVSIIITHH103 pKa = 6.94PKK105 pKa = 10.12LDD107 pKa = 3.36AQAIYY112 pKa = 11.0DD113 pKa = 4.61EE114 pKa = 4.31ITKK117 pKa = 10.43LVSICNEE124 pKa = 3.49FNVAYY129 pKa = 9.89DD130 pKa = 3.25GWGTQFEE137 pKa = 4.49DD138 pKa = 4.2EE139 pKa = 4.37EE140 pKa = 4.49

Molecular weight: 16.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A662ZF70|A0A662ZF70_9GAMM 30S ribosomal protein S13 OS=Ruminobacter amylophilus OX=867 GN=rpsM PE=3 SV=1
MM1 pKa = 7.71AIVNCKK7 pKa = 7.47PTSPGRR13 pKa = 11.84RR14 pKa = 11.84HH15 pKa = 4.78VVKK18 pKa = 10.53IVTPDD23 pKa = 3.53LYY25 pKa = 10.97KK26 pKa = 10.66GAPFAGLVEE35 pKa = 4.86EE36 pKa = 4.33KK37 pKa = 10.53RR38 pKa = 11.84KK39 pKa = 8.46TGGRR43 pKa = 11.84NNYY46 pKa = 8.87GRR48 pKa = 11.84ITTRR52 pKa = 11.84HH53 pKa = 5.49IGGGHH58 pKa = 4.82KK59 pKa = 9.56QRR61 pKa = 11.84YY62 pKa = 7.5RR63 pKa = 11.84VIDD66 pKa = 4.15FKK68 pKa = 10.86RR69 pKa = 11.84VKK71 pKa = 10.5DD72 pKa = 4.14GIPAKK77 pKa = 9.65VEE79 pKa = 4.13RR80 pKa = 11.84IEE82 pKa = 4.01YY83 pKa = 10.08DD84 pKa = 3.39PNRR87 pKa = 11.84SSHH90 pKa = 6.42IALICYY96 pKa = 10.01ADD98 pKa = 3.72GVRR101 pKa = 11.84SYY103 pKa = 11.35ILAPKK108 pKa = 9.2GLKK111 pKa = 10.52AGDD114 pKa = 3.78VVEE117 pKa = 5.13SGAHH121 pKa = 5.57ASIKK125 pKa = 10.4VGNALPLRR133 pKa = 11.84NIPVGTNVHH142 pKa = 5.86SVEE145 pKa = 4.47LYY147 pKa = 9.41PGKK150 pKa = 10.3GAQFARR156 pKa = 11.84SAGAFCQILARR167 pKa = 11.84EE168 pKa = 4.11GDD170 pKa = 4.3YY171 pKa = 10.07VTLRR175 pKa = 11.84MRR177 pKa = 11.84SGEE180 pKa = 4.01MRR182 pKa = 11.84RR183 pKa = 11.84VLADD187 pKa = 3.11GRR189 pKa = 11.84ATIGEE194 pKa = 4.41VGNSEE199 pKa = 4.09HH200 pKa = 7.01MLRR203 pKa = 11.84QLGKK207 pKa = 10.35AGAKK211 pKa = 8.46RR212 pKa = 11.84WLGIRR217 pKa = 11.84PTVRR221 pKa = 11.84GMSMNPIDD229 pKa = 4.36HH230 pKa = 6.77PHH232 pKa = 6.54GGGEE236 pKa = 4.12GRR238 pKa = 11.84NKK240 pKa = 10.26GIQPRR245 pKa = 11.84SPWGTLCKK253 pKa = 10.26GYY255 pKa = 8.48KK256 pKa = 7.12TRR258 pKa = 11.84KK259 pKa = 8.97NKK261 pKa = 9.12RR262 pKa = 11.84TDD264 pKa = 2.7KK265 pKa = 10.46YY266 pKa = 10.07IVRR269 pKa = 11.84RR270 pKa = 11.84RR271 pKa = 11.84DD272 pKa = 3.06KK273 pKa = 11.22

Molecular weight: 30.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2275

0

2275

767510

20

2529

337.4

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.312 ± 0.053

1.475 ± 0.021

6.014 ± 0.042

6.708 ± 0.055

4.304 ± 0.033

6.587 ± 0.05

1.875 ± 0.022

7.141 ± 0.045

6.586 ± 0.05

8.961 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.6 ± 0.024

5.275 ± 0.039

3.511 ± 0.028

2.921 ± 0.027

4.845 ± 0.048

7.124 ± 0.046

5.235 ± 0.042

6.835 ± 0.048

0.851 ± 0.016

3.84 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski