Taibaiella soli
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4294 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W2BJB6|A0A2W2BJB6_9BACT Uncharacterized protein OS=Taibaiella soli OX=1649169 GN=DN068_07365 PE=4 SV=1
MM1 pKa = 7.27 GGCVATDD8 pKa = 4.53 RR9 pKa = 11.84 ITVDD13 pKa = 3.56 GNRR16 pKa = 11.84 VGYY19 pKa = 7.82 MCRR22 pKa = 11.84 TEE24 pKa = 4.96 PINDD28 pKa = 4.63 LDD30 pKa = 4.94 SGWQFLAGDD39 pKa = 4.27 EE40 pKa = 4.3 SQEE43 pKa = 4.18 YY44 pKa = 10.93 VDD46 pKa = 4.14 VADD49 pKa = 3.78 NSGVYY54 pKa = 9.84 DD55 pKa = 3.63 VNTIANYY62 pKa = 10.27 DD63 pKa = 3.65 PEE65 pKa = 6.15 IIPFLHH71 pKa = 6.51 FPIGSEE77 pKa = 4.0 FEE79 pKa = 3.94 RR80 pKa = 11.84 DD81 pKa = 3.44 EE82 pKa = 4.24 VSGNLTQIRR91 pKa = 3.88
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.748
IPC2_protein 3.872
IPC_protein 3.821
Toseland 3.605
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.643
Grimsley 3.528
Solomon 3.795
Lehninger 3.745
Nozaki 3.935
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.855
Protein with the highest isoelectric point:
>tr|A0A2W2BV27|A0A2W2BV27_9BACT Probable membrane transporter protein OS=Taibaiella soli OX=1649169 GN=DN068_16475 PE=3 SV=1
MM1 pKa = 7.64 SFPSDD6 pKa = 3.47 LTNSQWKK13 pKa = 9.83 AISAFLPVKK22 pKa = 9.98 TKK24 pKa = 10.74 GIHH27 pKa = 5.38 NLRR30 pKa = 11.84 SIINALFYY38 pKa = 10.26 IVKK41 pKa = 9.0 TGCPWRR47 pKa = 11.84 YY48 pKa = 9.39 LPRR51 pKa = 11.84 CFPLWQSVYY60 pKa = 7.84 YY61 pKa = 9.94 HH62 pKa = 6.23 FRR64 pKa = 11.84 RR65 pKa = 11.84 LQSIGVLNRR74 pKa = 11.84 LMSKK78 pKa = 10.11 FRR80 pKa = 11.84 TLLRR84 pKa = 11.84 VNAGKK89 pKa = 10.35 SPQPSVALIDD99 pKa = 3.79 SQSIRR104 pKa = 11.84 TAAGVSKK111 pKa = 10.95 SKK113 pKa = 10.27 GWDD116 pKa = 3.17 GAKK119 pKa = 10.06 RR120 pKa = 11.84 LVGRR124 pKa = 11.84 KK125 pKa = 7.85 RR126 pKa = 11.84 HH127 pKa = 5.72 LVTDD131 pKa = 3.96 TLGLPLALCVTSASASDD148 pKa = 3.92 RR149 pKa = 11.84 EE150 pKa = 4.62 GMAAMTPYY158 pKa = 11.02 LNRR161 pKa = 11.84 IYY163 pKa = 10.82 NLKK166 pKa = 8.84 TLFADD171 pKa = 3.56 SGYY174 pKa = 9.61 MGIHH178 pKa = 5.77 ITDD181 pKa = 3.34 ARR183 pKa = 11.84 LIITSRR189 pKa = 11.84 KK190 pKa = 9.87 AIADD194 pKa = 3.57 KK195 pKa = 10.95 CNSLSGVLTEE205 pKa = 4.67 TSGTKK210 pKa = 8.76 PKK212 pKa = 9.92 RR213 pKa = 11.84 WVVEE217 pKa = 4.61 RR218 pKa = 11.84 SFAWIGHH225 pKa = 4.35 YY226 pKa = 9.92 RR227 pKa = 11.84 RR228 pKa = 11.84 LAKK231 pKa = 10.54 DD232 pKa = 3.76 FEE234 pKa = 4.46 RR235 pKa = 11.84 HH236 pKa = 4.41 TASSEE241 pKa = 3.76 AMIKK245 pKa = 10.03 IVFISIMLRR254 pKa = 11.84 RR255 pKa = 11.84 LNVVV259 pKa = 3.04
Molecular weight: 29.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.332
IPC2_protein 9.867
IPC_protein 10.643
Toseland 10.833
ProMoST 10.511
Dawson 10.921
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 11.213
Grimsley 10.965
Solomon 11.038
Lehninger 10.994
Nozaki 10.818
DTASelect 10.613
Thurlkill 10.818
EMBOSS 11.228
Sillero 10.847
Patrickios 10.921
IPC_peptide 11.038
IPC2_peptide 9.619
IPC2.peptide.svr19 8.46
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4294
0
4294
1541204
26
3735
358.9
39.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.956 ± 0.042
1.016 ± 0.017
5.281 ± 0.032
5.055 ± 0.055
4.674 ± 0.028
7.041 ± 0.041
1.98 ± 0.021
6.768 ± 0.03
6.056 ± 0.051
8.857 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.531 ± 0.02
5.752 ± 0.044
4.018 ± 0.023
3.953 ± 0.022
3.821 ± 0.034
6.528 ± 0.042
6.651 ± 0.084
6.576 ± 0.032
1.223 ± 0.013
4.264 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here