Amino acid dipepetide frequency for Streptococcus phage Javan174

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.539AlaAla: 6.539 ± 1.431
0.43AlaCys: 0.43 ± 0.205
6.023AlaAsp: 6.023 ± 0.859
6.023AlaGlu: 6.023 ± 0.822
2.839AlaPhe: 2.839 ± 0.881
5.163AlaGly: 5.163 ± 0.914
0.774AlaHis: 0.774 ± 0.232
4.991AlaIle: 4.991 ± 0.607
7.142AlaLys: 7.142 ± 0.62
7.142AlaLeu: 7.142 ± 1.211
3.614AlaMet: 3.614 ± 0.908
4.044AlaAsn: 4.044 ± 0.632
1.979AlaPro: 1.979 ± 0.403
2.839AlaGln: 2.839 ± 0.63
3.614AlaArg: 3.614 ± 1.057
4.991AlaSer: 4.991 ± 1.067
4.302AlaThr: 4.302 ± 0.552
6.281AlaVal: 6.281 ± 1.446
0.774AlaTrp: 0.774 ± 0.203
2.581AlaTyr: 2.581 ± 0.479
0.0AlaXaa: 0.0 ± 0.0
Cys
0.172CysAla: 0.172 ± 0.117
0.086CysCys: 0.086 ± 0.088
0.344CysAsp: 0.344 ± 0.159
0.43CysGlu: 0.43 ± 0.181
0.172CysPhe: 0.172 ± 0.112
0.258CysGly: 0.258 ± 0.147
0.0CysHis: 0.0 ± 0.0
0.172CysIle: 0.172 ± 0.111
0.172CysLys: 0.172 ± 0.131
0.602CysLeu: 0.602 ± 0.243
0.0CysMet: 0.0 ± 0.0
0.086CysAsn: 0.086 ± 0.081
0.086CysPro: 0.086 ± 0.094
0.086CysGln: 0.086 ± 0.077
0.172CysArg: 0.172 ± 0.113
0.43CysSer: 0.43 ± 0.222
0.172CysThr: 0.172 ± 0.114
0.344CysVal: 0.344 ± 0.168
0.086CysTrp: 0.086 ± 0.095
0.172CysTyr: 0.172 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
4.818AspAla: 4.818 ± 0.987
0.0AspCys: 0.0 ± 0.0
6.023AspAsp: 6.023 ± 0.728
5.421AspGlu: 5.421 ± 0.965
3.27AspPhe: 3.27 ± 0.518
5.765AspGly: 5.765 ± 0.811
0.602AspHis: 0.602 ± 0.191
3.7AspIle: 3.7 ± 0.474
4.818AspLys: 4.818 ± 0.569
4.732AspLeu: 4.732 ± 0.668
1.205AspMet: 1.205 ± 0.303
4.388AspAsn: 4.388 ± 0.574
1.119AspPro: 1.119 ± 0.377
1.205AspGln: 1.205 ± 0.368
2.495AspArg: 2.495 ± 0.555
3.442AspSer: 3.442 ± 0.585
4.388AspThr: 4.388 ± 0.62
3.184AspVal: 3.184 ± 0.542
0.602AspTrp: 0.602 ± 0.189
3.442AspTyr: 3.442 ± 0.743
0.0AspXaa: 0.0 ± 0.0
Glu
5.163GluAla: 5.163 ± 0.679
0.258GluCys: 0.258 ± 0.131
2.925GluAsp: 2.925 ± 0.576
3.872GluGlu: 3.872 ± 0.759
2.925GluPhe: 2.925 ± 0.41
2.581GluGly: 2.581 ± 0.439
1.205GluHis: 1.205 ± 0.309
3.786GluIle: 3.786 ± 0.528
4.646GluLys: 4.646 ± 0.654
7.572GluLeu: 7.572 ± 1.17
2.753GluMet: 2.753 ± 0.609
3.786GluAsn: 3.786 ± 0.557
1.377GluPro: 1.377 ± 0.379
4.216GluGln: 4.216 ± 0.593
2.925GluArg: 2.925 ± 0.459
2.667GluSer: 2.667 ± 0.469
3.442GluThr: 3.442 ± 0.681
4.818GluVal: 4.818 ± 0.777
0.86GluTrp: 0.86 ± 0.274
1.893GluTyr: 1.893 ± 0.394
0.0GluXaa: 0.0 ± 0.0
Phe
2.151PheAla: 2.151 ± 0.331
0.258PheCys: 0.258 ± 0.138
3.356PheAsp: 3.356 ± 0.631
3.184PheGlu: 3.184 ± 0.555
0.86PhePhe: 0.86 ± 0.261
3.27PheGly: 3.27 ± 0.726
0.688PheHis: 0.688 ± 0.226
2.065PheIle: 2.065 ± 0.414
2.409PheLys: 2.409 ± 0.624
2.667PheLeu: 2.667 ± 0.401
0.946PheMet: 0.946 ± 0.235
2.925PheAsn: 2.925 ± 0.462
0.774PhePro: 0.774 ± 0.216
1.119PheGln: 1.119 ± 0.342
1.463PheArg: 1.463 ± 0.376
3.528PheSer: 3.528 ± 0.572
2.581PheThr: 2.581 ± 0.484
2.667PheVal: 2.667 ± 0.38
0.516PheTrp: 0.516 ± 0.189
1.291PheTyr: 1.291 ± 0.334
0.0PheXaa: 0.0 ± 0.0
Gly
5.077GlyAla: 5.077 ± 1.282
0.43GlyCys: 0.43 ± 0.186
4.56GlyAsp: 4.56 ± 1.081
3.872GlyGlu: 3.872 ± 0.595
3.528GlyPhe: 3.528 ± 0.626
4.474GlyGly: 4.474 ± 0.465
1.119GlyHis: 1.119 ± 0.31
5.335GlyIle: 5.335 ± 0.774
5.421GlyLys: 5.421 ± 0.643
5.765GlyLeu: 5.765 ± 0.939
1.979GlyMet: 1.979 ± 0.437
4.044GlyAsn: 4.044 ± 0.781
0.172GlyPro: 0.172 ± 0.107
4.388GlyGln: 4.388 ± 0.615
3.614GlyArg: 3.614 ± 0.693
3.958GlySer: 3.958 ± 1.079
5.249GlyThr: 5.249 ± 0.842
5.851GlyVal: 5.851 ± 0.812
0.688GlyTrp: 0.688 ± 0.202
2.925GlyTyr: 2.925 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
1.119HisAla: 1.119 ± 0.241
0.086HisCys: 0.086 ± 0.097
0.774HisAsp: 0.774 ± 0.26
1.119HisGlu: 1.119 ± 0.252
0.688HisPhe: 0.688 ± 0.219
0.86HisGly: 0.86 ± 0.308
0.086HisHis: 0.086 ± 0.077
1.033HisIle: 1.033 ± 0.282
1.033HisLys: 1.033 ± 0.365
1.033HisLeu: 1.033 ± 0.306
0.344HisMet: 0.344 ± 0.169
0.86HisAsn: 0.86 ± 0.269
0.602HisPro: 0.602 ± 0.26
0.43HisGln: 0.43 ± 0.162
0.774HisArg: 0.774 ± 0.264
1.119HisSer: 1.119 ± 0.299
1.205HisThr: 1.205 ± 0.343
0.86HisVal: 0.86 ± 0.246
0.516HisTrp: 0.516 ± 0.229
0.86HisTyr: 0.86 ± 0.332
0.0HisXaa: 0.0 ± 0.0
Ile
5.421IleAla: 5.421 ± 0.725
0.172IleCys: 0.172 ± 0.122
4.388IleAsp: 4.388 ± 0.528
4.646IleGlu: 4.646 ± 0.687
1.979IlePhe: 1.979 ± 0.445
4.732IleGly: 4.732 ± 0.639
1.291IleHis: 1.291 ± 0.293
3.786IleIle: 3.786 ± 0.556
4.904IleLys: 4.904 ± 0.648
3.786IleLeu: 3.786 ± 0.642
0.602IleMet: 0.602 ± 0.228
3.442IleAsn: 3.442 ± 0.436
2.237IlePro: 2.237 ± 0.549
2.581IleGln: 2.581 ± 0.391
2.581IleArg: 2.581 ± 0.419
4.818IleSer: 4.818 ± 0.622
4.56IleThr: 4.56 ± 0.646
3.356IleVal: 3.356 ± 0.578
1.119IleTrp: 1.119 ± 0.338
1.979IleTyr: 1.979 ± 0.321
0.0IleXaa: 0.0 ± 0.0
Lys
6.625LysAla: 6.625 ± 0.845
0.086LysCys: 0.086 ± 0.097
4.474LysAsp: 4.474 ± 0.911
5.421LysGlu: 5.421 ± 0.877
1.979LysPhe: 1.979 ± 0.291
4.904LysGly: 4.904 ± 0.627
1.291LysHis: 1.291 ± 0.352
4.302LysIle: 4.302 ± 0.524
5.593LysLys: 5.593 ± 0.796
5.765LysLeu: 5.765 ± 0.954
2.925LysMet: 2.925 ± 0.573
3.786LysAsn: 3.786 ± 0.437
2.323LysPro: 2.323 ± 0.42
2.495LysGln: 2.495 ± 0.45
4.044LysArg: 4.044 ± 0.767
4.474LysSer: 4.474 ± 0.562
5.077LysThr: 5.077 ± 0.554
4.818LysVal: 4.818 ± 0.701
0.602LysTrp: 0.602 ± 0.201
1.979LysTyr: 1.979 ± 0.377
0.0LysXaa: 0.0 ± 0.0
Leu
8.002LeuAla: 8.002 ± 1.025
0.43LeuCys: 0.43 ± 0.223
6.023LeuAsp: 6.023 ± 0.71
5.507LeuGlu: 5.507 ± 0.619
2.581LeuPhe: 2.581 ± 0.425
5.679LeuGly: 5.679 ± 0.854
0.688LeuHis: 0.688 ± 0.261
4.13LeuIle: 4.13 ± 0.547
7.572LeuLys: 7.572 ± 0.866
5.851LeuLeu: 5.851 ± 0.583
1.033LeuMet: 1.033 ± 0.303
4.216LeuAsn: 4.216 ± 0.75
2.581LeuPro: 2.581 ± 0.52
2.667LeuGln: 2.667 ± 0.414
2.323LeuArg: 2.323 ± 0.504
6.281LeuSer: 6.281 ± 0.664
5.077LeuThr: 5.077 ± 0.648
5.421LeuVal: 5.421 ± 0.727
0.43LeuTrp: 0.43 ± 0.19
3.012LeuTyr: 3.012 ± 0.535
0.0LeuXaa: 0.0 ± 0.0
Met
2.581MetAla: 2.581 ± 0.493
0.172MetCys: 0.172 ± 0.118
0.688MetAsp: 0.688 ± 0.225
1.033MetGlu: 1.033 ± 0.291
0.688MetPhe: 0.688 ± 0.211
1.291MetGly: 1.291 ± 0.404
0.086MetHis: 0.086 ± 0.097
1.291MetIle: 1.291 ± 0.407
1.635MetLys: 1.635 ± 0.408
1.893MetLeu: 1.893 ± 0.457
0.258MetMet: 0.258 ± 0.132
0.86MetAsn: 0.86 ± 0.255
0.688MetPro: 0.688 ± 0.245
1.463MetGln: 1.463 ± 0.443
1.205MetArg: 1.205 ± 0.321
1.893MetSer: 1.893 ± 0.33
2.839MetThr: 2.839 ± 0.487
1.549MetVal: 1.549 ± 0.401
0.344MetTrp: 0.344 ± 0.136
0.946MetTyr: 0.946 ± 0.363
0.0MetXaa: 0.0 ± 0.0
Asn
4.818AsnAla: 4.818 ± 0.807
0.086AsnCys: 0.086 ± 0.079
2.839AsnAsp: 2.839 ± 0.54
2.839AsnGlu: 2.839 ± 0.73
1.635AsnPhe: 1.635 ± 0.337
4.904AsnGly: 4.904 ± 0.734
0.688AsnHis: 0.688 ± 0.284
3.872AsnIle: 3.872 ± 0.759
3.614AsnLys: 3.614 ± 0.579
4.56AsnLeu: 4.56 ± 0.634
0.946AsnMet: 0.946 ± 0.295
3.184AsnAsn: 3.184 ± 0.542
2.409AsnPro: 2.409 ± 0.515
2.925AsnGln: 2.925 ± 0.544
1.549AsnArg: 1.549 ± 0.332
3.958AsnSer: 3.958 ± 0.585
3.098AsnThr: 3.098 ± 0.498
3.27AsnVal: 3.27 ± 0.709
0.774AsnTrp: 0.774 ± 0.262
2.151AsnTyr: 2.151 ± 0.478
0.0AsnXaa: 0.0 ± 0.0
Pro
2.151ProAla: 2.151 ± 0.352
0.172ProCys: 0.172 ± 0.108
2.495ProAsp: 2.495 ± 0.615
1.721ProGlu: 1.721 ± 0.515
1.463ProPhe: 1.463 ± 0.325
1.377ProGly: 1.377 ± 0.406
0.774ProHis: 0.774 ± 0.269
1.807ProIle: 1.807 ± 0.375
2.151ProLys: 2.151 ± 0.489
1.549ProLeu: 1.549 ± 0.427
0.688ProMet: 0.688 ± 0.21
1.377ProAsn: 1.377 ± 0.356
0.688ProPro: 0.688 ± 0.222
0.86ProGln: 0.86 ± 0.308
0.688ProArg: 0.688 ± 0.315
2.151ProSer: 2.151 ± 0.34
1.721ProThr: 1.721 ± 0.396
2.409ProVal: 2.409 ± 0.487
0.172ProTrp: 0.172 ± 0.124
1.463ProTyr: 1.463 ± 0.43
0.0ProXaa: 0.0 ± 0.0
Gln
3.614GlnAla: 3.614 ± 0.715
0.172GlnCys: 0.172 ± 0.11
1.291GlnAsp: 1.291 ± 0.333
2.323GlnGlu: 2.323 ± 0.472
1.549GlnPhe: 1.549 ± 0.364
4.56GlnGly: 4.56 ± 0.949
0.602GlnHis: 0.602 ± 0.232
3.012GlnIle: 3.012 ± 0.541
3.27GlnLys: 3.27 ± 0.5
3.786GlnLeu: 3.786 ± 0.479
0.946GlnMet: 0.946 ± 0.222
2.237GlnAsn: 2.237 ± 0.544
0.86GlnPro: 0.86 ± 0.332
1.893GlnGln: 1.893 ± 0.463
1.377GlnArg: 1.377 ± 0.329
2.839GlnSer: 2.839 ± 0.57
2.151GlnThr: 2.151 ± 0.53
2.237GlnVal: 2.237 ± 0.335
0.516GlnTrp: 0.516 ± 0.171
1.377GlnTyr: 1.377 ± 0.337
0.0GlnXaa: 0.0 ± 0.0
Arg
2.667ArgAla: 2.667 ± 0.508
0.172ArgCys: 0.172 ± 0.114
1.721ArgAsp: 1.721 ± 0.36
2.151ArgGlu: 2.151 ± 0.495
1.463ArgPhe: 1.463 ± 0.345
2.839ArgGly: 2.839 ± 0.519
0.774ArgHis: 0.774 ± 0.244
2.409ArgIle: 2.409 ± 0.498
2.495ArgLys: 2.495 ± 0.577
3.528ArgLeu: 3.528 ± 0.556
0.86ArgMet: 0.86 ± 0.243
2.495ArgAsn: 2.495 ± 0.468
1.377ArgPro: 1.377 ± 0.31
1.635ArgGln: 1.635 ± 0.35
1.549ArgArg: 1.549 ± 0.405
2.065ArgSer: 2.065 ± 0.412
2.581ArgThr: 2.581 ± 0.51
3.7ArgVal: 3.7 ± 1.101
1.033ArgTrp: 1.033 ± 0.345
3.356ArgTyr: 3.356 ± 0.677
0.0ArgXaa: 0.0 ± 0.0
Ser
5.421SerAla: 5.421 ± 1.055
0.344SerCys: 0.344 ± 0.164
3.872SerAsp: 3.872 ± 0.646
3.442SerGlu: 3.442 ± 0.546
2.581SerPhe: 2.581 ± 0.605
6.023SerGly: 6.023 ± 1.638
1.205SerHis: 1.205 ± 0.349
3.872SerIle: 3.872 ± 0.665
3.442SerLys: 3.442 ± 0.578
5.851SerLeu: 5.851 ± 0.7
0.946SerMet: 0.946 ± 0.293
3.958SerAsn: 3.958 ± 0.587
2.495SerPro: 2.495 ± 0.334
2.581SerGln: 2.581 ± 0.486
2.151SerArg: 2.151 ± 0.563
5.077SerSer: 5.077 ± 0.824
4.904SerThr: 4.904 ± 0.8
4.388SerVal: 4.388 ± 0.708
1.377SerTrp: 1.377 ± 0.309
2.925SerTyr: 2.925 ± 0.635
0.0SerXaa: 0.0 ± 0.0
Thr
5.507ThrAla: 5.507 ± 0.743
0.258ThrCys: 0.258 ± 0.135
3.528ThrAsp: 3.528 ± 0.667
3.442ThrGlu: 3.442 ± 0.738
3.098ThrPhe: 3.098 ± 0.527
5.335ThrGly: 5.335 ± 0.662
1.463ThrHis: 1.463 ± 0.435
5.507ThrIle: 5.507 ± 0.604
4.904ThrLys: 4.904 ± 0.66
5.335ThrLeu: 5.335 ± 0.709
1.033ThrMet: 1.033 ± 0.341
2.237ThrAsn: 2.237 ± 0.364
2.925ThrPro: 2.925 ± 0.502
2.839ThrGln: 2.839 ± 0.628
2.409ThrArg: 2.409 ± 0.508
3.27ThrSer: 3.27 ± 0.69
4.732ThrThr: 4.732 ± 0.679
5.851ThrVal: 5.851 ± 0.823
0.86ThrTrp: 0.86 ± 0.28
2.753ThrTyr: 2.753 ± 0.611
0.0ThrXaa: 0.0 ± 0.0
Val
6.797ValAla: 6.797 ± 1.463
0.086ValCys: 0.086 ± 0.092
6.281ValAsp: 6.281 ± 0.653
4.646ValGlu: 4.646 ± 0.719
3.098ValPhe: 3.098 ± 0.484
4.904ValGly: 4.904 ± 0.866
0.946ValHis: 0.946 ± 0.285
4.216ValIle: 4.216 ± 0.589
3.872ValLys: 3.872 ± 0.46
3.442ValLeu: 3.442 ± 0.629
1.205ValMet: 1.205 ± 0.297
3.356ValAsn: 3.356 ± 0.471
1.807ValPro: 1.807 ± 0.348
1.549ValGln: 1.549 ± 0.388
2.581ValArg: 2.581 ± 0.597
6.625ValSer: 6.625 ± 1.318
5.249ValThr: 5.249 ± 0.666
4.818ValVal: 4.818 ± 0.554
0.86ValTrp: 0.86 ± 0.269
3.184ValTyr: 3.184 ± 0.657
0.0ValXaa: 0.0 ± 0.0
Trp
1.119TrpAla: 1.119 ± 0.203
0.0TrpCys: 0.0 ± 0.0
0.258TrpAsp: 0.258 ± 0.144
0.86TrpGlu: 0.86 ± 0.198
0.516TrpPhe: 0.516 ± 0.183
0.86TrpGly: 0.86 ± 0.28
0.258TrpHis: 0.258 ± 0.153
0.86TrpIle: 0.86 ± 0.212
0.946TrpLys: 0.946 ± 0.199
1.205TrpLeu: 1.205 ± 0.37
0.0TrpMet: 0.0 ± 0.0
0.43TrpAsn: 0.43 ± 0.158
0.0TrpPro: 0.0 ± 0.0
0.86TrpGln: 0.86 ± 0.297
0.86TrpArg: 0.86 ± 0.241
1.033TrpSer: 1.033 ± 0.309
0.774TrpThr: 0.774 ± 0.298
1.119TrpVal: 1.119 ± 0.292
0.344TrpTrp: 0.344 ± 0.175
0.602TrpTyr: 0.602 ± 0.281
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.323TyrAla: 2.323 ± 0.493
0.344TyrCys: 0.344 ± 0.188
2.753TyrAsp: 2.753 ± 0.565
1.721TyrGlu: 1.721 ± 0.343
1.807TyrPhe: 1.807 ± 0.523
2.667TyrGly: 2.667 ± 0.594
0.86TyrHis: 0.86 ± 0.306
2.237TyrIle: 2.237 ± 0.444
3.012TyrLys: 3.012 ± 0.613
3.442TyrLeu: 3.442 ± 0.701
1.033TyrMet: 1.033 ± 0.258
2.409TyrAsn: 2.409 ± 0.495
1.377TyrPro: 1.377 ± 0.367
1.979TyrGln: 1.979 ± 0.351
2.495TyrArg: 2.495 ± 0.416
2.237TyrSer: 2.237 ± 0.354
3.184TyrThr: 3.184 ± 0.522
2.581TyrVal: 2.581 ± 0.393
0.43TyrTrp: 0.43 ± 0.16
1.807TyrTyr: 1.807 ± 0.443
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (11623 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski