TM7 phylum sp. oral taxon 351
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 797 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A563C6B8|A0A563C6B8_9BACT Uncharacterized protein OS=TM7 phylum sp. oral taxon 351 OX=713053 GN=EUA77_03720 PE=4 SV=1
MM1 pKa = 7.7 FEE3 pKa = 4.47 EE4 pKa = 4.63 YY5 pKa = 11.07 NNGVPDD11 pKa = 4.21 RR12 pKa = 11.84 LRR14 pKa = 11.84 QVTAEE19 pKa = 4.15 TMSPEE24 pKa = 3.95 ALYY27 pKa = 10.1 TLDD30 pKa = 3.73 TYY32 pKa = 11.83 SDD34 pKa = 3.78 VSPQDD39 pKa = 3.13 VIYY42 pKa = 10.24 IDD44 pKa = 3.65 EE45 pKa = 4.33 SRR47 pKa = 11.84 AEE49 pKa = 3.99 GSGRR53 pKa = 11.84 LIISKK58 pKa = 9.42 GQSVKK63 pKa = 10.76 LLEE66 pKa = 4.52 EE67 pKa = 5.2 ADD69 pKa = 4.21 LSTPDD74 pKa = 4.44 LVILMSVWQRR84 pKa = 11.84 NDD86 pKa = 3.0 QGDD89 pKa = 3.84 LCRR92 pKa = 11.84 GIVADD97 pKa = 3.59 CRR99 pKa = 11.84 FYY101 pKa = 11.24 SGCFDD106 pKa = 3.81 AEE108 pKa = 4.31 YY109 pKa = 10.8 GDD111 pKa = 5.46 EE112 pKa = 5.33 EE113 pKa = 5.87 IDD115 pKa = 3.96 DD116 pKa = 4.55 CDD118 pKa = 5.74 GIDD121 pKa = 4.3 DD122 pKa = 5.19 EE123 pKa = 5.79 IYY125 pKa = 11.18 NKK127 pKa = 10.43 DD128 pKa = 3.58 DD129 pKa = 3.62 EE130 pKa = 4.6 QVISAVVDD138 pKa = 3.44 YY139 pKa = 11.56 GDD141 pKa = 4.33 DD142 pKa = 3.74 GKK144 pKa = 11.68 VKK146 pKa = 10.03 IWGDD150 pKa = 3.34 PTFFDD155 pKa = 3.9 AARR158 pKa = 11.84 YY159 pKa = 7.2 MAGLVDD165 pKa = 3.81 NEE167 pKa = 4.52 SIEE170 pKa = 4.21 KK171 pKa = 10.96 NEE173 pKa = 3.89 VCASSHH179 pKa = 5.66 EE180 pKa = 4.19 LQDD183 pKa = 3.61 CRR185 pKa = 11.84 SYY187 pKa = 10.56 QHH189 pKa = 6.35 NVGASALPCSLIFLALASAII209 pKa = 3.95
Molecular weight: 23.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.872
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.719
EMBOSS 3.808
Sillero 3.999
Patrickios 1.24
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.887
Protein with the highest isoelectric point:
>tr|A0A563CAN7|A0A563CAN7_9BACT Holliday junction ATP-dependent DNA helicase RuvB OS=TM7 phylum sp. oral taxon 351 OX=713053 GN=ruvB PE=3 SV=1
MM1 pKa = 7.29 QDD3 pKa = 3.0 EE4 pKa = 4.84 SRR6 pKa = 11.84 TSIRR10 pKa = 11.84 ISQPSSARR18 pKa = 11.84 TRR20 pKa = 11.84 MAQSQASYY28 pKa = 10.69 RR29 pKa = 11.84 RR30 pKa = 11.84 MDD32 pKa = 4.13 FAPVQTVRR40 pKa = 11.84 RR41 pKa = 11.84 AAASIPTDD49 pKa = 3.32 RR50 pKa = 11.84 DD51 pKa = 3.44 LEE53 pKa = 4.02 RR54 pKa = 11.84 HH55 pKa = 6.27 RR56 pKa = 11.84 IAKK59 pKa = 9.75 QEE61 pKa = 3.89 MQRR64 pKa = 11.84 RR65 pKa = 11.84 EE66 pKa = 4.49 AIRR69 pKa = 11.84 QQIEE73 pKa = 3.78 EE74 pKa = 4.06 QNRR77 pKa = 11.84 LKK79 pKa = 10.88 NLNQRR84 pKa = 11.84 RR85 pKa = 11.84 IEE87 pKa = 3.95 AARR90 pKa = 11.84 QEE92 pKa = 4.1 LSEE95 pKa = 4.11 YY96 pKa = 9.98 QAAEE100 pKa = 4.1 EE101 pKa = 3.98 QRR103 pKa = 11.84 TRR105 pKa = 11.84 ALEE108 pKa = 3.91 AEE110 pKa = 4.24 RR111 pKa = 11.84 QRR113 pKa = 11.84 AIEE116 pKa = 4.16 SQRR119 pKa = 11.84 LAQRR123 pKa = 11.84 EE124 pKa = 4.26 ALEE127 pKa = 4.07 KK128 pKa = 9.16 MAARR132 pKa = 11.84 RR133 pKa = 11.84 RR134 pKa = 11.84 AMEE137 pKa = 3.46 QEE139 pKa = 3.3 ARR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 QIEE146 pKa = 3.95 EE147 pKa = 3.46 QDD149 pKa = 2.94 RR150 pKa = 11.84 KK151 pKa = 8.99 MRR153 pKa = 11.84 AMQEE157 pKa = 3.79 MQRR160 pKa = 11.84 QQEE163 pKa = 3.78 ASKK166 pKa = 10.56 INVSRR171 pKa = 11.84 RR172 pKa = 11.84 ISVAPVQTSPFKK184 pKa = 10.94 KK185 pKa = 8.78 VTNPAARR192 pKa = 11.84 IQARR196 pKa = 11.84 SVDD199 pKa = 3.92 MQAKK203 pKa = 5.59 TTISVSDD210 pKa = 3.82 TQAKK214 pKa = 8.22 PALRR218 pKa = 11.84 SAGDD222 pKa = 3.74 TKK224 pKa = 11.03 VLPASRR230 pKa = 11.84 IQVVASPNRR239 pKa = 11.84 KK240 pKa = 8.7 LFGRR244 pKa = 11.84 IRR246 pKa = 11.84 PAGPSRR252 pKa = 11.84 QVPQDD257 pKa = 3.39 EE258 pKa = 4.16 QDD260 pKa = 3.84 PLVQSARR267 pKa = 11.84 NSHH270 pKa = 6.4 IEE272 pKa = 3.77 LSTSGASITSDD283 pKa = 3.09 SDD285 pKa = 4.24 LEE287 pKa = 4.38 DD288 pKa = 3.34 MLYY291 pKa = 10.87 DD292 pKa = 3.64 AFEE295 pKa = 5.67 DD296 pKa = 4.08 STSDD300 pKa = 3.19 TPEE303 pKa = 3.99 RR304 pKa = 11.84 LSSKK308 pKa = 10.37 SRR310 pKa = 11.84 NQSQNSS316 pKa = 3.35
Molecular weight: 36.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.443
IPC_protein 10.423
Toseland 10.818
ProMoST 10.701
Dawson 10.862
Bjellqvist 10.628
Wikipedia 11.125
Rodwell 10.891
Grimsley 10.891
Solomon 11.096
Lehninger 11.052
Nozaki 10.789
DTASelect 10.628
Thurlkill 10.804
EMBOSS 11.242
Sillero 10.804
Patrickios 10.613
IPC_peptide 11.096
IPC2_peptide 9.502
IPC2.peptide.svr19 8.964
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
797
0
797
235844
38
1280
295.9
33.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.545 ± 0.08
0.639 ± 0.024
5.837 ± 0.069
6.884 ± 0.099
4.046 ± 0.061
6.418 ± 0.083
1.749 ± 0.038
7.543 ± 0.08
7.425 ± 0.088
9.246 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.369 ± 0.04
4.987 ± 0.08
3.641 ± 0.055
3.685 ± 0.061
4.765 ± 0.071
6.663 ± 0.082
5.691 ± 0.078
6.643 ± 0.069
0.896 ± 0.032
3.327 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here