Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541) (Haloarcula mukohataei)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Halomicrobium; Halomicrobium mukohataei

Average proteome isoelectric point is 4.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7P3E9|C7P3E9_HALMD Isocitrate dehydrogenase (NADP(+)) OS=Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541) OX=485914 GN=Hmuk_1506 PE=4 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84EE3 pKa = 3.53TRR5 pKa = 11.84RR6 pKa = 11.84NILRR10 pKa = 11.84KK11 pKa = 9.86ASALSALAIGVSGTASAADD30 pKa = 3.88CSDD33 pKa = 3.34VQQWQSGTAYY43 pKa = 10.5NGGDD47 pKa = 2.72RR48 pKa = 11.84VVYY51 pKa = 10.75DD52 pKa = 3.67DD53 pKa = 4.79VLWEE57 pKa = 4.22AEE59 pKa = 3.43WWTQANEE66 pKa = 4.14PEE68 pKa = 4.44EE69 pKa = 4.52SDD71 pKa = 4.81SVWTKK76 pKa = 10.72IGDD79 pKa = 3.73CSGDD83 pKa = 3.75TEE85 pKa = 4.43NEE87 pKa = 3.81APVASFTASISTPAPGEE104 pKa = 4.21SVTFDD109 pKa = 3.33ASGSSDD115 pKa = 3.34PDD117 pKa = 3.63GSVSSYY123 pKa = 11.34AWDD126 pKa = 4.12FGDD129 pKa = 4.89GDD131 pKa = 4.05TATGQTASHH140 pKa = 7.09TYY142 pKa = 10.56GSAGDD147 pKa = 3.77YY148 pKa = 9.42TVTLTVTDD156 pKa = 4.44DD157 pKa = 4.3DD158 pKa = 4.8GASGTASTTVSVSEE172 pKa = 4.78SDD174 pKa = 3.72NEE176 pKa = 3.94APNASFTVSPSSPTTGEE193 pKa = 4.16SVTVDD198 pKa = 3.12AADD201 pKa = 3.87SSDD204 pKa = 3.55ADD206 pKa = 3.92GSISSYY212 pKa = 11.3AWDD215 pKa = 4.43FGDD218 pKa = 5.12DD219 pKa = 3.6ATASGQTATHH229 pKa = 7.08TYY231 pKa = 10.24DD232 pKa = 2.94SSGEE236 pKa = 3.94YY237 pKa = 10.21TITLTVTDD245 pKa = 5.2DD246 pKa = 5.38DD247 pKa = 5.15GATDD251 pKa = 3.72TNATTVSVGGDD262 pKa = 3.06GGEE265 pKa = 4.14CSGVSEE271 pKa = 4.35WDD273 pKa = 2.99SGTTYY278 pKa = 10.47TGGDD282 pKa = 3.22QVIYY286 pKa = 10.84DD287 pKa = 3.74GTLWEE292 pKa = 4.48AKK294 pKa = 8.03WWSKK298 pKa = 10.92GDD300 pKa = 3.72EE301 pKa = 4.26PSSDD305 pKa = 3.19GGPWKK310 pKa = 10.52QITACGPPEE319 pKa = 4.42PVTKK323 pKa = 9.9TLADD327 pKa = 4.21LVPKK331 pKa = 10.43PGDD334 pKa = 3.09ITTADD339 pKa = 3.97DD340 pKa = 4.02GFEE343 pKa = 4.17ITSSTTIVAEE353 pKa = 4.42GSGTEE358 pKa = 3.93VGQYY362 pKa = 10.54LADD365 pKa = 4.01LLGPATGFDD374 pKa = 3.92LSVEE378 pKa = 4.47SGSSASADD386 pKa = 3.76SIALLLNGAPSSVGDD401 pKa = 3.63EE402 pKa = 4.45GYY404 pKa = 11.29EE405 pKa = 3.82MSVDD409 pKa = 3.45SDD411 pKa = 3.8GVTIRR416 pKa = 11.84ANEE419 pKa = 3.91AAGLFYY425 pKa = 10.75GVQSLRR431 pKa = 11.84QVLPAAVEE439 pKa = 3.94ADD441 pKa = 3.43TDD443 pKa = 3.85QSVDD447 pKa = 2.95WVVPGGSVTDD457 pKa = 3.74TPRR460 pKa = 11.84FEE462 pKa = 4.44YY463 pKa = 10.22RR464 pKa = 11.84GAMLDD469 pKa = 3.6VARR472 pKa = 11.84HH473 pKa = 5.36FFDD476 pKa = 3.39KK477 pKa = 11.08SVVKK481 pKa = 10.18EE482 pKa = 4.67FIDD485 pKa = 3.45QVAAYY490 pKa = 8.87KK491 pKa = 10.31INHH494 pKa = 6.12LHH496 pKa = 6.57LHH498 pKa = 5.7LTDD501 pKa = 3.87DD502 pKa = 3.6QGWRR506 pKa = 11.84IEE508 pKa = 3.96IDD510 pKa = 3.18DD511 pKa = 4.27WPNLTDD517 pKa = 3.55EE518 pKa = 4.88GADD521 pKa = 3.69SEE523 pKa = 4.75VDD525 pKa = 3.58GGPGGYY531 pKa = 7.59FTKK534 pKa = 10.58ADD536 pKa = 3.68YY537 pKa = 11.12QEE539 pKa = 4.75IIQYY543 pKa = 10.2AQDD546 pKa = 2.85RR547 pKa = 11.84HH548 pKa = 4.47MTVVPEE554 pKa = 3.8IDD556 pKa = 3.67MPGHH560 pKa = 6.09TGAALEE566 pKa = 4.93SYY568 pKa = 11.36AEE570 pKa = 4.37LNCDD574 pKa = 3.26DD575 pKa = 4.48TKK577 pKa = 11.11RR578 pKa = 11.84EE579 pKa = 4.0EE580 pKa = 4.05DD581 pKa = 3.1TGINVGDD588 pKa = 3.86TTLCMDD594 pKa = 5.14DD595 pKa = 3.45EE596 pKa = 4.72HH597 pKa = 8.73KK598 pKa = 9.48EE599 pKa = 4.07TSLQFAADD607 pKa = 3.93VISAVAEE614 pKa = 4.34MTDD617 pKa = 3.05GPYY620 pKa = 10.54FHH622 pKa = 7.23VGGDD626 pKa = 3.6EE627 pKa = 4.44ADD629 pKa = 3.62VLSDD633 pKa = 3.28AKK635 pKa = 11.15YY636 pKa = 10.89EE637 pKa = 4.05EE638 pKa = 5.31FIDD641 pKa = 4.24AVLPMIEE648 pKa = 4.88DD649 pKa = 3.52AGKK652 pKa = 8.33TPIGWHH658 pKa = 5.76QIASTEE664 pKa = 4.16PVTSALLHH672 pKa = 5.26YY673 pKa = 9.69WGTDD677 pKa = 3.02AQAPEE682 pKa = 3.96VAARR686 pKa = 11.84ASEE689 pKa = 4.17GNDD692 pKa = 3.18VIASPAHH699 pKa = 6.45LAYY702 pKa = 10.6LDD704 pKa = 3.38QDD706 pKa = 3.67YY707 pKa = 11.02NYY709 pKa = 10.33QDD711 pKa = 4.21GVGQDD716 pKa = 2.89WAGPVSVEE724 pKa = 5.49DD725 pKa = 4.55AYY727 pKa = 10.07TWDD730 pKa = 3.86PGSYY734 pKa = 9.22IDD736 pKa = 5.86GVDD739 pKa = 3.52EE740 pKa = 4.45SSVAGVEE747 pKa = 4.14APLWTEE753 pKa = 3.88FVEE756 pKa = 4.51TQDD759 pKa = 4.26DD760 pKa = 3.7IEE762 pKa = 5.01YY763 pKa = 9.81MVFPRR768 pKa = 11.84LAAIAEE774 pKa = 4.55LGWSSSSDD782 pKa = 2.87IGDD785 pKa = 3.66FDD787 pKa = 5.26AFSQRR792 pKa = 11.84LALQGPRR799 pKa = 11.84WAQANVNYY807 pKa = 9.74YY808 pKa = 10.54QSDD811 pKa = 3.82LVDD814 pKa = 3.41WQQ816 pKa = 3.44

Molecular weight: 86.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7NXY6|C7NXY6_HALMD Uncharacterized protein OS=Halomicrobium mukohataei (strain ATCC 700874 / DSM 12286 / JCM 9738 / NCIMB 13541) OX=485914 GN=Hmuk_0440 PE=4 SV=1
MM1 pKa = 7.48AVLLAIATAPLTGIALVLAALIVALLARR29 pKa = 11.84AARR32 pKa = 11.84PRR34 pKa = 11.84RR35 pKa = 11.84PATSPRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84TTVVPGTTRR52 pKa = 2.91

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3343

0

3343

957314

30

1589

286.4

31.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.084 ± 0.054

0.735 ± 0.013

8.656 ± 0.06

8.292 ± 0.053

3.158 ± 0.027

8.481 ± 0.039

1.972 ± 0.021

3.926 ± 0.032

1.674 ± 0.023

8.899 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.017

2.158 ± 0.024

4.616 ± 0.028

2.736 ± 0.03

6.655 ± 0.044

5.595 ± 0.033

6.677 ± 0.038

9.179 ± 0.045

1.149 ± 0.016

2.66 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski