Oceanibaculum indicum P24
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3754 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K2KHU1|K2KHU1_9PROT Ribokinase-like domain-containing protein OS=Oceanibaculum indicum P24 OX=1207063 GN=P24_06671 PE=4 SV=1
MM1 pKa = 6.69 TTTTAVTASGTSYY14 pKa = 10.7 IDD16 pKa = 3.89 SLLHH20 pKa = 4.8 GQKK23 pKa = 9.5 WDD25 pKa = 3.69 GTTLTYY31 pKa = 10.87 AFPDD35 pKa = 3.13 AAANVGYY42 pKa = 10.14 SIDD45 pKa = 3.62 GAYY48 pKa = 7.82 FTALSGAEE56 pKa = 3.75 QTAFEE61 pKa = 4.71 AVLQAWARR69 pKa = 11.84 VSGLTFTEE77 pKa = 4.41 VAAADD82 pKa = 3.78 AATADD87 pKa = 3.52 ISVYY91 pKa = 9.4 WYY93 pKa = 10.26 RR94 pKa = 11.84 SPDD97 pKa = 3.31 NLTARR102 pKa = 11.84 VVTFPDD108 pKa = 3.79 GTVEE112 pKa = 4.83 GGDD115 pKa = 3.33 IQLGSGINGGDD126 pKa = 3.35 LSSPGSYY133 pKa = 9.95 SHH135 pKa = 7.51 FIAMHH140 pKa = 5.78 EE141 pKa = 4.28 LGHH144 pKa = 6.86 ALGLKK149 pKa = 9.75 HH150 pKa = 5.66 PHH152 pKa = 5.47 EE153 pKa = 4.84 VEE155 pKa = 4.08 GSFPADD161 pKa = 2.52 TGMSVEE167 pKa = 4.48 LSVMSYY173 pKa = 10.83 VSFTGGSVSSYY184 pKa = 10.87 SIADD188 pKa = 3.41 GSYY191 pKa = 8.3 PTAPMLNDD199 pKa = 3.28 IAAIQYY205 pKa = 10.58 LYY207 pKa = 11.13 GVNTNALTANLGDD220 pKa = 3.4 TTYY223 pKa = 10.51 TYY225 pKa = 11.31 DD226 pKa = 3.76 PGAAIIFQARR236 pKa = 11.84 WDD238 pKa = 3.63 GGGYY242 pKa = 8.4 DD243 pKa = 3.57 TFNFASYY250 pKa = 8.4 GTDD253 pKa = 2.87 LSVDD257 pKa = 3.9 LRR259 pKa = 11.84 PGQWIDD265 pKa = 4.11 LGGQYY270 pKa = 11.11 AVLDD274 pKa = 3.76 TGDD277 pKa = 3.64 INVTPLGNIAIPYY290 pKa = 8.17 LHH292 pKa = 7.22 EE293 pKa = 5.13 GNTAYY298 pKa = 10.82 LIEE301 pKa = 4.26 AAYY304 pKa = 10.07 GGSGNDD310 pKa = 3.52 LIVGNEE316 pKa = 3.82 ANNLLRR322 pKa = 11.84 GNGGNDD328 pKa = 3.49 TLRR331 pKa = 11.84 GGAGTDD337 pKa = 3.48 TLVGSAGQDD346 pKa = 2.96 TFDD349 pKa = 4.08 FTDD352 pKa = 4.42 GAASSNTVSDD362 pKa = 4.22 FTADD366 pKa = 3.44 DD367 pKa = 4.36 TIVLRR372 pKa = 11.84 DD373 pKa = 3.67 AVVGGIVRR381 pKa = 11.84 GNDD384 pKa = 3.25 GATLAQGEE392 pKa = 4.91 VSFAWNGVYY401 pKa = 10.34 NVLYY405 pKa = 10.75 VGLDD409 pKa = 3.26 ATAGSDD415 pKa = 3.85 LQIVLANSTVFDD427 pKa = 4.03 NLSLSGNTISFIADD441 pKa = 3.52 TVAPEE446 pKa = 4.17 FLSGTGPADD455 pKa = 3.32 NAVGVARR462 pKa = 11.84 DD463 pKa = 3.82 ATPTLIFNEE472 pKa = 4.36 EE473 pKa = 3.77 VSAGTGEE480 pKa = 4.01 FIIYY484 pKa = 10.36 NVTNPSVVKK493 pKa = 9.67 TIAANSAAVTGWGTYY508 pKa = 10.02 RR509 pKa = 11.84 LTIDD513 pKa = 4.12 IDD515 pKa = 4.06 PDD517 pKa = 3.63 GLTPLPYY524 pKa = 10.01 GAEE527 pKa = 3.79 IAIMWDD533 pKa = 3.16 EE534 pKa = 4.14 SAVRR538 pKa = 11.84 DD539 pKa = 3.99 LAGNPLAEE547 pKa = 4.39 NTSEE551 pKa = 4.04 TLYY554 pKa = 11.36 SFIANVGPTATASAANVAHH573 pKa = 6.55 TAAGQTSYY581 pKa = 11.34 SFTVTYY587 pKa = 10.91 SDD589 pKa = 4.25 ADD591 pKa = 3.62 GTIDD595 pKa = 4.67 DD596 pKa = 4.66 TSIDD600 pKa = 4.02 TNDD603 pKa = 3.55 VTVTAPDD610 pKa = 3.65 TSTLTVTGAVWNGDD624 pKa = 3.8 TNTATYY630 pKa = 8.85 TVEE633 pKa = 4.13 VPGGNGWEE641 pKa = 3.99 AALEE645 pKa = 4.07 GTYY648 pKa = 10.21 TIGLVEE654 pKa = 5.26 GEE656 pKa = 4.29 VTDD659 pKa = 3.53 NHH661 pKa = 6.68 GDD663 pKa = 3.74 GVAGNANADD672 pKa = 3.84 SFTVDD677 pKa = 3.34 LTVPPTPDD685 pKa = 4.0 PGPGPSLPPPTDD697 pKa = 3.21 TSVGGVDD704 pKa = 2.6 IATRR708 pKa = 11.84 YY709 pKa = 8.18 STDD712 pKa = 2.98 ASDD715 pKa = 3.66 RR716 pKa = 11.84 QIEE719 pKa = 4.57 TVTITPNGTQQTTQGVPLAGPASNPVLSGTLPSTVSVTVTRR760 pKa = 11.84 PSAPISSTALVNSLLAEE777 pKa = 4.27 VPFGIVLPDD786 pKa = 4.15 DD787 pKa = 4.1 PSLPVLAALRR797 pKa = 11.84 PNVPITARR805 pKa = 11.84 TITMTPYY812 pKa = 9.85 GTDD815 pKa = 3.52 DD816 pKa = 4.26 LLEE819 pKa = 4.45 AMIIEE824 pKa = 5.03 GSPDD828 pKa = 2.96 SGQASGIVLDD838 pKa = 4.73 ARR840 pKa = 11.84 NLPSGAPIQLNNVDD854 pKa = 3.61 VAIISGDD861 pKa = 3.7 VVVSGGAGPSIVLGDD876 pKa = 4.08 AGSQNIVLGEE886 pKa = 4.09 GTDD889 pKa = 3.93 YY890 pKa = 11.63 LHH892 pKa = 7.22 GGAGNDD898 pKa = 4.07 TIGSATGSDD907 pKa = 3.27 TLLGGEE913 pKa = 4.67 GDD915 pKa = 3.91 DD916 pKa = 4.77 SVFGGMNGDD925 pKa = 4.04 SIEE928 pKa = 4.34 GGEE931 pKa = 4.44 GNDD934 pKa = 3.26 ILRR937 pKa = 11.84 GGKK940 pKa = 9.36 GHH942 pKa = 7.79 DD943 pKa = 4.12 SLDD946 pKa = 3.55 GGAGDD951 pKa = 5.55 DD952 pKa = 3.44 ILYY955 pKa = 10.82 SGFGDD960 pKa = 3.64 DD961 pKa = 4.01 TLTGGEE967 pKa = 4.21 GADD970 pKa = 3.41 LFVLRR975 pKa = 11.84 GFDD978 pKa = 3.68 ANFAGAVLKK987 pKa = 10.12 ATVTDD992 pKa = 4.89 FAQGTDD998 pKa = 2.92 RR999 pKa = 11.84 FAVEE1003 pKa = 4.27 NVSVAALEE1011 pKa = 4.24 AALALQSVTEE1021 pKa = 4.15 AGVVIEE1027 pKa = 4.27 VAGATLTFIGISQLTAADD1045 pKa = 3.77 IDD1047 pKa = 3.63 QSFYY1051 pKa = 11.76 AA1052 pKa = 4.75
Molecular weight: 107.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.554
IPC_protein 3.617
Toseland 3.376
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.567
Rodwell 3.439
Grimsley 3.274
Solomon 3.617
Lehninger 3.579
Nozaki 3.719
DTASelect 4.012
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.745
Patrickios 0.68
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|K2KLP3|K2KLP3_9PROT YlxR domain-containing protein OS=Oceanibaculum indicum P24 OX=1207063 GN=P24_02326 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 8.7 IVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.49 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.32 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.9 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3754
0
3754
1192065
27
6700
317.5
34.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.631 ± 0.062
0.837 ± 0.012
5.65 ± 0.036
5.898 ± 0.039
3.546 ± 0.027
8.851 ± 0.04
1.968 ± 0.018
5.051 ± 0.033
3.25 ± 0.039
10.715 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.612 ± 0.023
2.417 ± 0.022
5.328 ± 0.037
3.197 ± 0.023
7.12 ± 0.041
4.956 ± 0.021
5.17 ± 0.028
7.209 ± 0.036
1.288 ± 0.016
2.306 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here