Amino acid dipepetide frequency for Actinoplanes friuliensis DSM 7358

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.255AlaAla: 22.255 ± 0.155	0.877AlaCys: 0.877 ± 0.018	8.543AlaAsp: 8.543 ± 0.06	7.951AlaGlu: 7.951 ± 0.069	3.486AlaPhe: 3.486 ± 0.039	14.359AlaGly: 14.359 ± 0.086	2.304AlaHis: 2.304 ± 0.034	4.297AlaIle: 4.297 ± 0.044	2.65AlaLys: 2.65 ± 0.043	14.086AlaLeu: 14.086 ± 0.101	2.505AlaMet: 2.505 ± 0.034	2.277AlaAsn: 2.277 ± 0.034	6.597AlaPro: 6.597 ± 0.072	3.729AlaGln: 3.729 ± 0.043	9.354AlaArg: 9.354 ± 0.069	5.929AlaSer: 5.929 ± 0.058	8.111AlaThr: 8.111 ± 0.074	12.387AlaVal: 12.387 ± 0.087	1.932AlaTrp: 1.932 ± 0.031	2.837AlaTyr: 2.837 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	0.85CysAla: 0.85 ± 0.018	0.071CysCys: 0.071 ± 0.005	0.421CysAsp: 0.421 ± 0.011	0.291CysGlu: 0.291 ± 0.011	0.198CysPhe: 0.198 ± 0.008	0.78CysGly: 0.78 ± 0.017	0.156CysHis: 0.156 ± 0.008	0.156CysIle: 0.156 ± 0.007	0.092CysLys: 0.092 ± 0.006	0.65CysLeu: 0.65 ± 0.014	0.084CysMet: 0.084 ± 0.005	0.144CysAsn: 0.144 ± 0.009	0.399CysPro: 0.399 ± 0.014	0.149CysGln: 0.149 ± 0.008	0.506CysArg: 0.506 ± 0.015	0.388CysSer: 0.388 ± 0.011	0.456CysThr: 0.456 ± 0.019	0.547CysVal: 0.547 ± 0.016	0.115CysTrp: 0.115 ± 0.008	0.142CysTyr: 0.142 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	7.247AspAla: 7.247 ± 0.064	0.38AspCys: 0.38 ± 0.014	3.986AspAsp: 3.986 ± 0.043	3.752AspGlu: 3.752 ± 0.041	1.652AspPhe: 1.652 ± 0.025	6.155AspGly: 6.155 ± 0.052	1.339AspHis: 1.339 ± 0.023	1.845AspIle: 1.845 ± 0.028	1.202AspLys: 1.202 ± 0.025	6.942AspLeu: 6.942 ± 0.044	0.731AspMet: 0.731 ± 0.018	1.233AspAsn: 1.233 ± 0.025	4.568AspPro: 4.568 ± 0.043	1.85AspGln: 1.85 ± 0.027	5.015AspArg: 5.015 ± 0.051	2.547AspSer: 2.547 ± 0.032	3.131AspThr: 3.131 ± 0.033	5.028AspVal: 5.028 ± 0.046	0.947AspTrp: 0.947 ± 0.018	1.189AspTyr: 1.189 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	6.56GluAla: 6.56 ± 0.063	0.285GluCys: 0.285 ± 0.01	2.461GluAsp: 2.461 ± 0.032	2.545GluGlu: 2.545 ± 0.039	1.473GluPhe: 1.473 ± 0.023	3.342GluGly: 3.342 ± 0.04	1.274GluHis: 1.274 ± 0.021	2.301GluIle: 2.301 ± 0.031	1.2GluLys: 1.2 ± 0.024	6.325GluLeu: 6.325 ± 0.054	0.855GluMet: 0.855 ± 0.019	0.975GluAsn: 0.975 ± 0.017	3.258GluPro: 3.258 ± 0.047	2.223GluGln: 2.223 ± 0.03	4.48GluArg: 4.48 ± 0.047	2.397GluSer: 2.397 ± 0.03	2.82GluThr: 2.82 ± 0.039	4.501GluVal: 4.501 ± 0.043	0.698GluTrp: 0.698 ± 0.014	0.971GluTyr: 0.971 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.98PheAla: 3.98 ± 0.041	0.256PheCys: 0.256 ± 0.011	2.081PheAsp: 2.081 ± 0.027	1.37PheGlu: 1.37 ± 0.022	0.899PhePhe: 0.899 ± 0.02	3.04PheGly: 3.04 ± 0.035	0.573PheHis: 0.573 ± 0.014	0.801PheIle: 0.801 ± 0.017	0.528PheLys: 0.528 ± 0.016	2.651PheLeu: 2.651 ± 0.04	0.363PheMet: 0.363 ± 0.012	0.601PheAsn: 0.601 ± 0.016	1.378PhePro: 1.378 ± 0.022	0.692PheGln: 0.692 ± 0.015	1.837PheArg: 1.837 ± 0.024	1.539PheSer: 1.539 ± 0.026	2.148PheThr: 2.148 ± 0.03	2.537PheVal: 2.537 ± 0.032	0.457PheTrp: 0.457 ± 0.014	0.624PheTyr: 0.624 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	10.602GlyAla: 10.602 ± 0.076	0.697GlyCys: 0.697 ± 0.02	5.503GlyAsp: 5.503 ± 0.057	4.548GlyGlu: 4.548 ± 0.042	3.021GlyPhe: 3.021 ± 0.037	8.397GlyGly: 8.397 ± 0.084	2.034GlyHis: 2.034 ± 0.03	3.481GlyIle: 3.481 ± 0.04	2.332GlyLys: 2.332 ± 0.04	9.836GlyLeu: 9.836 ± 0.076	1.767GlyMet: 1.767 ± 0.032	1.991GlyAsn: 1.991 ± 0.037	4.996GlyPro: 4.996 ± 0.047	2.645GlyGln: 2.645 ± 0.039	7.234GlyArg: 7.234 ± 0.06	5.313GlySer: 5.313 ± 0.053	6.303GlyThr: 6.303 ± 0.076	7.729GlyVal: 7.729 ± 0.063	1.812GlyTrp: 1.812 ± 0.027	2.378GlyTyr: 2.378 ± 0.036	0.0GlyXaa: 0.0 ± 0.0
His	2.276HisAla: 2.276 ± 0.032	0.152HisCys: 0.152 ± 0.008	1.346HisAsp: 1.346 ± 0.026	1.083HisGlu: 1.083 ± 0.022	0.597HisPhe: 0.597 ± 0.017	1.97HisGly: 1.97 ± 0.031	0.595HisHis: 0.595 ± 0.017	0.592HisIle: 0.592 ± 0.015	0.29HisLys: 0.29 ± 0.012	2.37HisLeu: 2.37 ± 0.032	0.258HisMet: 0.258 ± 0.011	0.403HisAsn: 0.403 ± 0.011	1.557HisPro: 1.557 ± 0.026	0.633HisGln: 0.633 ± 0.017	1.842HisArg: 1.842 ± 0.028	0.912HisSer: 0.912 ± 0.021	1.068HisThr: 1.068 ± 0.022	1.585HisVal: 1.585 ± 0.025	0.317HisTrp: 0.317 ± 0.01	0.433HisTyr: 0.433 ± 0.011	0.0HisXaa: 0.0 ± 0.0
Ile	5.356IleAla: 5.356 ± 0.052	0.286IleCys: 0.286 ± 0.01	2.562IleAsp: 2.562 ± 0.031	2.074IleGlu: 2.074 ± 0.032	1.003IlePhe: 1.003 ± 0.021	3.822IleGly: 3.822 ± 0.047	0.571IleHis: 0.571 ± 0.015	1.185IleIle: 1.185 ± 0.027	0.779IleLys: 0.779 ± 0.018	2.858IleLeu: 2.858 ± 0.036	0.519IleMet: 0.519 ± 0.014	0.885IleAsn: 0.885 ± 0.02	1.757IlePro: 1.757 ± 0.027	0.771IleGln: 0.771 ± 0.018	2.451IleArg: 2.451 ± 0.032	2.041IleSer: 2.041 ± 0.028	2.69IleThr: 2.69 ± 0.035	3.325IleVal: 3.325 ± 0.04	0.472IleTrp: 0.472 ± 0.013	0.671IleTyr: 0.671 ± 0.012	0.0IleXaa: 0.0 ± 0.0
Lys	2.591LysAla: 2.591 ± 0.044	0.127LysCys: 0.127 ± 0.008	1.064LysAsp: 1.064 ± 0.024	0.852LysGlu: 0.852 ± 0.02	0.543LysPhe: 0.543 ± 0.017	1.454LysGly: 1.454 ± 0.027	0.404LysHis: 0.404 ± 0.012	0.975LysIle: 0.975 ± 0.024	0.69LysLys: 0.69 ± 0.026	2.158LysLeu: 2.158 ± 0.035	0.383LysMet: 0.383 ± 0.013	0.513LysAsn: 0.513 ± 0.016	1.323LysPro: 1.323 ± 0.026	0.675LysGln: 0.675 ± 0.017	1.379LysArg: 1.379 ± 0.027	1.047LysSer: 1.047 ± 0.022	1.262LysThr: 1.262 ± 0.027	1.87LysVal: 1.87 ± 0.031	0.283LysTrp: 0.283 ± 0.013	0.448LysTyr: 0.448 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	15.343LeuAla: 15.343 ± 0.112	0.671LeuCys: 0.671 ± 0.014	6.825LeuAsp: 6.825 ± 0.061	4.376LeuGlu: 4.376 ± 0.046	2.687LeuPhe: 2.687 ± 0.039	9.503LeuGly: 9.503 ± 0.074	2.201LeuHis: 2.201 ± 0.03	4.14LeuIle: 4.14 ± 0.042	1.75LeuLys: 1.75 ± 0.03	11.559LeuLeu: 11.559 ± 0.117	1.529LeuMet: 1.529 ± 0.024	1.859LeuAsn: 1.859 ± 0.025	6.417LeuPro: 6.417 ± 0.053	2.431LeuGln: 2.431 ± 0.031	8.548LeuArg: 8.548 ± 0.07	5.678LeuSer: 5.678 ± 0.047	7.491LeuThr: 7.491 ± 0.062	9.282LeuVal: 9.282 ± 0.079	1.291LeuTrp: 1.291 ± 0.02	1.742LeuTyr: 1.742 ± 0.024	0.0LeuXaa: 0.0 ± 0.0
Met	2.078MetAla: 2.078 ± 0.034	0.105MetCys: 0.105 ± 0.006	0.765MetAsp: 0.765 ± 0.016	0.598MetGlu: 0.598 ± 0.017	0.473MetPhe: 0.473 ± 0.014	1.124MetGly: 1.124 ± 0.022	0.329MetHis: 0.329 ± 0.011	0.816MetIle: 0.816 ± 0.018	0.395MetLys: 0.395 ± 0.014	1.845MetLeu: 1.845 ± 0.028	0.31MetMet: 0.31 ± 0.012	0.389MetAsn: 0.389 ± 0.011	1.11MetPro: 1.11 ± 0.02	0.467MetGln: 0.467 ± 0.013	1.362MetArg: 1.362 ± 0.023	1.222MetSer: 1.222 ± 0.022	1.649MetThr: 1.649 ± 0.026	1.312MetVal: 1.312 ± 0.024	0.21MetTrp: 0.21 ± 0.009	0.295MetTyr: 0.295 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	2.438AsnAla: 2.438 ± 0.03	0.166AsnCys: 0.166 ± 0.008	1.117AsnAsp: 1.117 ± 0.021	0.833AsnGlu: 0.833 ± 0.018	0.571AsnPhe: 0.571 ± 0.017	2.195AsnGly: 2.195 ± 0.052	0.395AsnHis: 0.395 ± 0.012	0.665AsnIle: 0.665 ± 0.017	0.417AsnLys: 0.417 ± 0.014	1.955AsnLeu: 1.955 ± 0.029	0.315AsnMet: 0.315 ± 0.011	0.58AsnAsn: 0.58 ± 0.018	1.503AsnPro: 1.503 ± 0.024	0.611AsnGln: 0.611 ± 0.019	1.433AsnArg: 1.433 ± 0.025	1.001AsnSer: 1.001 ± 0.024	1.265AsnThr: 1.265 ± 0.026	1.543AsnVal: 1.543 ± 0.026	0.341AsnTrp: 0.341 ± 0.013	0.464AsnTyr: 0.464 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.298ProAla: 9.298 ± 0.092	0.259ProCys: 0.259 ± 0.01	4.406ProAsp: 4.406 ± 0.044	3.832ProGlu: 3.832 ± 0.044	1.525ProPhe: 1.525 ± 0.024	6.528ProGly: 6.528 ± 0.061	1.057ProHis: 1.057 ± 0.02	1.763ProIle: 1.763 ± 0.029	1.081ProLys: 1.081 ± 0.025	5.054ProLeu: 5.054 ± 0.046	1.026ProMet: 1.026 ± 0.02	0.996ProAsn: 0.996 ± 0.022	3.693ProPro: 3.693 ± 0.054	1.63ProGln: 1.63 ± 0.026	3.523ProArg: 3.523 ± 0.04	3.232ProSer: 3.232 ± 0.044	3.304ProThr: 3.304 ± 0.045	5.503ProVal: 5.503 ± 0.053	0.891ProTrp: 0.891 ± 0.021	1.227ProTyr: 1.227 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	3.838GlnAla: 3.838 ± 0.041	0.144GlnCys: 0.144 ± 0.007	1.319GlnAsp: 1.319 ± 0.023	1.252GlnGlu: 1.252 ± 0.021	0.801GlnPhe: 0.801 ± 0.018	2.047GlnGly: 2.047 ± 0.031	0.631GlnHis: 0.631 ± 0.017	1.248GlnIle: 1.248 ± 0.025	0.562GlnLys: 0.562 ± 0.015	3.307GlnLeu: 3.307 ± 0.038	0.478GlnMet: 0.478 ± 0.014	0.58GlnAsn: 0.58 ± 0.016	1.823GlnPro: 1.823 ± 0.033	1.28GlnGln: 1.28 ± 0.025	2.534GlnArg: 2.534 ± 0.031	1.376GlnSer: 1.376 ± 0.025	1.506GlnThr: 1.506 ± 0.026	2.673GlnVal: 2.673 ± 0.031	0.497GlnTrp: 0.497 ± 0.014	0.575GlnTyr: 0.575 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	9.061ArgAla: 9.061 ± 0.073	0.453ArgCys: 0.453 ± 0.014	4.341ArgAsp: 4.341 ± 0.049	4.056ArgGlu: 4.056 ± 0.039	2.321ArgPhe: 2.321 ± 0.031	5.306ArgGly: 5.306 ± 0.054	1.895ArgHis: 1.895 ± 0.028	3.274ArgIle: 3.274 ± 0.034	1.54ArgLys: 1.54 ± 0.024	8.714ArgLeu: 8.714 ± 0.071	1.742ArgMet: 1.742 ± 0.026	1.489ArgAsn: 1.489 ± 0.022	4.663ArgPro: 4.663 ± 0.051	2.34ArgGln: 2.34 ± 0.031	7.399ArgArg: 7.399 ± 0.072	4.116ArgSer: 4.116 ± 0.04	5.001ArgThr: 5.001 ± 0.041	5.697ArgVal: 5.697 ± 0.053	1.429ArgTrp: 1.429 ± 0.025	1.757ArgTyr: 1.757 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	6.94SerAla: 6.94 ± 0.068	0.359SerCys: 0.359 ± 0.013	2.741SerAsp: 2.741 ± 0.032	2.192SerGlu: 2.192 ± 0.032	1.641SerPhe: 1.641 ± 0.024	5.807SerGly: 5.807 ± 0.06	0.926SerHis: 0.926 ± 0.018	1.836SerIle: 1.836 ± 0.03	0.99SerLys: 0.99 ± 0.022	4.858SerLeu: 4.858 ± 0.043	1.151SerMet: 1.151 ± 0.018	0.985SerAsn: 0.985 ± 0.021	3.3SerPro: 3.3 ± 0.038	1.299SerGln: 1.299 ± 0.025	3.802SerArg: 3.802 ± 0.039	3.084SerSer: 3.084 ± 0.043	3.407SerThr: 3.407 ± 0.047	4.363SerVal: 4.363 ± 0.041	1.035SerTrp: 1.035 ± 0.021	1.22SerTyr: 1.22 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	9.084ThrAla: 9.084 ± 0.077	0.424ThrCys: 0.424 ± 0.016	3.822ThrAsp: 3.822 ± 0.037	3.126ThrGlu: 3.126 ± 0.037	1.933ThrPhe: 1.933 ± 0.03	7.012ThrGly: 7.012 ± 0.078	1.05ThrHis: 1.05 ± 0.02	2.399ThrIle: 2.399 ± 0.032	1.161ThrLys: 1.161 ± 0.025	5.976ThrLeu: 5.976 ± 0.053	1.063ThrMet: 1.063 ± 0.023	1.244ThrAsn: 1.244 ± 0.027	4.184ThrPro: 4.184 ± 0.058	1.421ThrGln: 1.421 ± 0.023	4.064ThrArg: 4.064 ± 0.042	3.399ThrSer: 3.399 ± 0.041	4.543ThrThr: 4.543 ± 0.065	6.517ThrVal: 6.517 ± 0.079	1.064ThrTrp: 1.064 ± 0.025	1.396ThrTyr: 1.396 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	12.124ValAla: 12.124 ± 0.085	0.597ValCys: 0.597 ± 0.014	5.253ValAsp: 5.253 ± 0.052	4.475ValGlu: 4.475 ± 0.039	2.345ValPhe: 2.345 ± 0.031	6.725ValGly: 6.725 ± 0.052	1.753ValHis: 1.753 ± 0.029	3.245ValIle: 3.245 ± 0.039	1.684ValLys: 1.684 ± 0.028	10.018ValLeu: 10.018 ± 0.088	1.272ValMet: 1.272 ± 0.022	1.767ValAsn: 1.767 ± 0.029	5.277ValPro: 5.277 ± 0.047	2.265ValGln: 2.265 ± 0.027	6.68ValArg: 6.68 ± 0.057	4.666ValSer: 4.666 ± 0.047	6.39ValThr: 6.39 ± 0.072	8.66ValVal: 8.66 ± 0.073	1.145ValTrp: 1.145 ± 0.021	1.497ValTyr: 1.497 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.71TrpAla: 1.71 ± 0.025	0.131TrpCys: 0.131 ± 0.007	0.767TrpAsp: 0.767 ± 0.019	0.655TrpGlu: 0.655 ± 0.015	0.531TrpPhe: 0.531 ± 0.015	0.996TrpGly: 0.996 ± 0.02	0.389TrpHis: 0.389 ± 0.013	0.61TrpIle: 0.61 ± 0.014	0.337TrpLys: 0.337 ± 0.011	1.892TrpLeu: 1.892 ± 0.03	0.298TrpMet: 0.298 ± 0.01	0.457TrpAsn: 0.457 ± 0.015	0.895TrpPro: 0.895 ± 0.02	0.686TrpGln: 0.686 ± 0.017	1.369TrpArg: 1.369 ± 0.026	1.057TrpSer: 1.057 ± 0.02	1.105TrpThr: 1.105 ± 0.023	1.074TrpVal: 1.074 ± 0.019	0.39TrpTrp: 0.39 ± 0.013	0.328TrpTyr: 0.328 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.834TyrAla: 2.834 ± 0.032	0.153TyrCys: 0.153 ± 0.008	1.417TyrAsp: 1.417 ± 0.027	1.083TyrGlu: 1.083 ± 0.024	0.656TyrPhe: 0.656 ± 0.014	2.057TyrGly: 2.057 ± 0.033	0.402TyrHis: 0.402 ± 0.012	0.45TyrIle: 0.45 ± 0.013	0.362TyrLys: 0.362 ± 0.013	2.271TyrLeu: 2.271 ± 0.032	0.202TyrMet: 0.202 ± 0.008	0.44TyrAsn: 0.44 ± 0.016	1.132TyrPro: 1.132 ± 0.022	0.671TyrGln: 0.671 ± 0.016	1.783TyrArg: 1.783 ± 0.031	0.993TyrSer: 0.993 ± 0.021	1.229TyrThr: 1.229 ± 0.023	1.705TyrVal: 1.705 ± 0.025	0.352TyrTrp: 0.352 ± 0.013	0.473TyrTyr: 0.473 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8558 proteins (2802956 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---