Wenzhou narna-like virus 8
Average proteome isoelectric point is 8.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIN7|A0A1L3KIN7_9VIRU RNA-dependent RNA polymerase OS=Wenzhou narna-like virus 8 OX=1923583 PE=4 SV=1
MM1 pKa = 7.46 RR2 pKa = 11.84 RR3 pKa = 11.84 EE4 pKa = 4.12 KK5 pKa = 10.57 PKK7 pKa = 10.91 SPPCKK12 pKa = 9.1 RR13 pKa = 11.84 KK14 pKa = 7.01 TWRR17 pKa = 11.84 TTRR20 pKa = 11.84 NSANRR25 pKa = 11.84 DD26 pKa = 2.89 ASADD30 pKa = 3.42 KK31 pKa = 11.09 NLISRR36 pKa = 11.84 KK37 pKa = 9.42 RR38 pKa = 11.84 LIHH41 pKa = 6.74 SFQTCLEE48 pKa = 3.62 VWMRR52 pKa = 11.84 KK53 pKa = 8.93 SFSFSIKK60 pKa = 10.29 HH61 pKa = 5.53 PGKK64 pKa = 10.47 FIEE67 pKa = 4.32 TEE69 pKa = 3.78 KK70 pKa = 10.07 FQKK73 pKa = 10.12 IFEE76 pKa = 4.87 GIPLKK81 pKa = 10.4 EE82 pKa = 3.64 LPKK85 pKa = 10.23 RR86 pKa = 11.84 FKK88 pKa = 10.82 HH89 pKa = 5.64 LLCDD93 pKa = 3.61 NFSVLTNQSCPPADD107 pKa = 3.93 YY108 pKa = 11.17 ARR110 pKa = 11.84 VPIKK114 pKa = 10.78 GLIAKK119 pKa = 9.58 CSRR122 pKa = 11.84 TSFHH126 pKa = 7.19 TIMCRR131 pKa = 11.84 LRR133 pKa = 11.84 SHH135 pKa = 6.29 KK136 pKa = 10.41 RR137 pKa = 11.84 YY138 pKa = 8.33 VACWNLLQCKK148 pKa = 9.45 SLAHH152 pKa = 5.78 EE153 pKa = 4.65 VPDD156 pKa = 5.05 EE157 pKa = 4.64 FIQDD161 pKa = 3.5 ALVKK165 pKa = 10.47 HH166 pKa = 6.28 RR167 pKa = 11.84 ATMEE171 pKa = 4.03 KK172 pKa = 10.07 EE173 pKa = 4.03 PEE175 pKa = 3.87 PLEE178 pKa = 4.36 IEE180 pKa = 4.11 VEE182 pKa = 3.66 EE183 pKa = 4.46 DD184 pKa = 2.83 FRR186 pKa = 11.84 HH187 pKa = 4.91 FVKK190 pKa = 10.69 PFVDD194 pKa = 3.81 QVIEE198 pKa = 4.07 QFDD201 pKa = 4.04 GKK203 pKa = 9.32 TSYY206 pKa = 7.91 PTNHH210 pKa = 5.97 SCVGFTRR217 pKa = 11.84 SGGGQLAAHH226 pKa = 6.25 SHH228 pKa = 7.22 RR229 pKa = 11.84 IRR231 pKa = 11.84 TSSGRR236 pKa = 11.84 RR237 pKa = 11.84 LPMGGRR243 pKa = 11.84 VGRR246 pKa = 11.84 KK247 pKa = 8.57 EE248 pKa = 3.91 PVVLTLEE255 pKa = 4.93 GPPSSGKK262 pKa = 8.56 STMVKK267 pKa = 10.38 YY268 pKa = 10.17 LVKK271 pKa = 10.08 EE272 pKa = 4.04 LQRR275 pKa = 11.84 QFQLKK280 pKa = 10.12 SDD282 pKa = 3.52 TVYY285 pKa = 10.79 YY286 pKa = 10.43 RR287 pKa = 11.84 SAATDD292 pKa = 2.66 HH293 pKa = 6.89 WDD295 pKa = 4.21 GYY297 pKa = 8.53 WGQPITVLDD306 pKa = 3.95 DD307 pKa = 3.41 VGAFASSMTQPSADD321 pKa = 3.78 LKK323 pKa = 11.11 EE324 pKa = 4.01 LLQLVSEE331 pKa = 4.82 CDD333 pKa = 3.61 YY334 pKa = 10.7 VLPMADD340 pKa = 4.5 LKK342 pKa = 11.5 DD343 pKa = 3.54 KK344 pKa = 10.77 GRR346 pKa = 11.84 KK347 pKa = 6.19 FTSPIIIMTTNYY359 pKa = 9.27 GARR362 pKa = 11.84 YY363 pKa = 7.68 NQTGFSCSAAIARR376 pKa = 11.84 RR377 pKa = 11.84 FGTVYY382 pKa = 10.32 RR383 pKa = 11.84 VVKK386 pKa = 9.57 GQAYY390 pKa = 7.62 DD391 pKa = 3.47 TYY393 pKa = 10.61 TQSTDD398 pKa = 3.24 QGCVDD403 pKa = 4.33 EE404 pKa = 5.05 MSHH407 pKa = 6.67 PLDD410 pKa = 3.56 SFVRR414 pKa = 11.84 KK415 pKa = 8.32 MRR417 pKa = 11.84 KK418 pKa = 9.16 DD419 pKa = 3.22 RR420 pKa = 11.84 PVSIRR425 pKa = 11.84 AMVNRR430 pKa = 11.84 LRR432 pKa = 11.84 TQYY435 pKa = 11.56 DD436 pKa = 3.58 EE437 pKa = 3.99 FHH439 pKa = 6.66 GNLRR443 pKa = 11.84 QEE445 pKa = 4.37 VLGMGTSLVFKK456 pKa = 10.94 KK457 pKa = 10.51 EE458 pKa = 4.09 DD459 pKa = 3.52 LSSLINTCEE468 pKa = 3.75 VTAIPEE474 pKa = 4.07 PLKK477 pKa = 10.86 VRR479 pKa = 11.84 TITRR483 pKa = 11.84 PEE485 pKa = 4.05 PLTYY489 pKa = 10.52 ALKK492 pKa = 10.22 PIQRR496 pKa = 11.84 AMFEE500 pKa = 3.8 ALKK503 pKa = 10.1 RR504 pKa = 11.84 WKK506 pKa = 10.24 CFQPCWDD513 pKa = 4.5 PNYY516 pKa = 10.87 SLQEE520 pKa = 3.77 LLRR523 pKa = 11.84 PEE525 pKa = 4.32 EE526 pKa = 4.65 GEE528 pKa = 4.15 TLLSGDD534 pKa = 3.9 YY535 pKa = 10.58 TSATDD540 pKa = 3.89 DD541 pKa = 3.43 LHH543 pKa = 6.92 PRR545 pKa = 11.84 LSQIVGEE552 pKa = 4.45 MIADD556 pKa = 3.99 RR557 pKa = 11.84 LGDD560 pKa = 3.85 GFLSDD565 pKa = 4.67 LIRR568 pKa = 11.84 WEE570 pKa = 4.33 SGPHH574 pKa = 6.4 LISYY578 pKa = 9.08 PKK580 pKa = 10.45 DD581 pKa = 3.28 SGLAAVWQTNGQLMGSLLSFPILCLANAFTMTRR614 pKa = 11.84 ATGTTLDD621 pKa = 3.34 TVRR624 pKa = 11.84 AVFHH628 pKa = 7.0 GDD630 pKa = 3.54 DD631 pKa = 3.31 IAAVATEE638 pKa = 4.09 SQISKK643 pKa = 9.37 WEE645 pKa = 4.05 KK646 pKa = 8.19 EE647 pKa = 3.95 ASSVGLSLSLGKK659 pKa = 10.42 NYY661 pKa = 10.09 VSRR664 pKa = 11.84 HH665 pKa = 4.49 FVSIDD670 pKa = 3.16 SQLFLWEE677 pKa = 4.8 DD678 pKa = 3.87 GEE680 pKa = 4.55 LVRR683 pKa = 11.84 KK684 pKa = 8.12 VTGKK688 pKa = 9.74 FRR690 pKa = 11.84 LVNRR694 pKa = 11.84 EE695 pKa = 3.76 AGEE698 pKa = 4.4 SCFEE702 pKa = 3.67 QALRR706 pKa = 11.84 QGFNKK711 pKa = 9.9 QLIRR715 pKa = 11.84 TLCKK719 pKa = 10.14 SQLAKK724 pKa = 10.34 SLRR727 pKa = 11.84 SLEE730 pKa = 4.24 VAVEE734 pKa = 3.91 QGGLGIQTTRR744 pKa = 11.84 PLTLWEE750 pKa = 3.63 KK751 pKa = 10.52 CYY753 pKa = 11.05 YY754 pKa = 9.78 LNRR757 pKa = 11.84 YY758 pKa = 7.2 QARR761 pKa = 11.84 TSCKK765 pKa = 10.18 EE766 pKa = 3.49 MGEE769 pKa = 4.4 GIRR772 pKa = 11.84 VPKK775 pKa = 9.98 EE776 pKa = 3.05 LAVRR780 pKa = 11.84 LRR782 pKa = 11.84 LTSVKK787 pKa = 11.04 SMMLPEE793 pKa = 4.74 EE794 pKa = 4.52 KK795 pKa = 10.09 PSEE798 pKa = 4.22 KK799 pKa = 10.46 NVNKK803 pKa = 10.07 QVWRR807 pKa = 11.84 LMKK810 pKa = 10.46 KK811 pKa = 9.82 CLKK814 pKa = 10.22 NSKK817 pKa = 10.06 FYY819 pKa = 11.32 EE820 pKa = 4.16 KK821 pKa = 10.61 VQNLSCSRR829 pKa = 11.84 PLSTLLTSVVRR840 pKa = 11.84 CDD842 pKa = 3.71 YY843 pKa = 11.12 SPQEE847 pKa = 3.99 LSRR850 pKa = 11.84 VYY852 pKa = 10.8 QQMFPMKK859 pKa = 10.39
Molecular weight: 97.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.797
IPC2_protein 8.624
IPC_protein 8.478
Toseland 9.194
ProMoST 9.092
Dawson 9.516
Bjellqvist 9.37
Wikipedia 9.663
Rodwell 9.78
Grimsley 9.545
Solomon 9.545
Lehninger 9.516
Nozaki 9.575
DTASelect 9.253
Thurlkill 9.414
EMBOSS 9.692
Sillero 9.575
Patrickios 4.952
IPC_peptide 9.545
IPC2_peptide 8.141
IPC2.peptide.svr19 7.793
Protein with the highest isoelectric point:
>tr|A0A1L3KIN7|A0A1L3KIN7_9VIRU RNA-dependent RNA polymerase OS=Wenzhou narna-like virus 8 OX=1923583 PE=4 SV=1
MM1 pKa = 7.46 RR2 pKa = 11.84 RR3 pKa = 11.84 EE4 pKa = 4.12 KK5 pKa = 10.57 PKK7 pKa = 10.91 SPPCKK12 pKa = 9.1 RR13 pKa = 11.84 KK14 pKa = 7.01 TWRR17 pKa = 11.84 TTRR20 pKa = 11.84 NSANRR25 pKa = 11.84 DD26 pKa = 2.89 ASADD30 pKa = 3.42 KK31 pKa = 11.09 NLISRR36 pKa = 11.84 KK37 pKa = 9.42 RR38 pKa = 11.84 LIHH41 pKa = 6.74 SFQTCLEE48 pKa = 3.62 VWMRR52 pKa = 11.84 KK53 pKa = 8.93 SFSFSIKK60 pKa = 10.29 HH61 pKa = 5.53 PGKK64 pKa = 10.47 FIEE67 pKa = 4.32 TEE69 pKa = 3.78 KK70 pKa = 10.07 FQKK73 pKa = 10.12 IFEE76 pKa = 4.87 GIPLKK81 pKa = 10.4 EE82 pKa = 3.64 LPKK85 pKa = 10.23 RR86 pKa = 11.84 FKK88 pKa = 10.82 HH89 pKa = 5.64 LLCDD93 pKa = 3.61 NFSVLTNQSCPPADD107 pKa = 3.93 YY108 pKa = 11.17 ARR110 pKa = 11.84 VPIKK114 pKa = 10.78 GLIAKK119 pKa = 9.58 CSRR122 pKa = 11.84 TSFHH126 pKa = 7.19 TIMCRR131 pKa = 11.84 LRR133 pKa = 11.84 SHH135 pKa = 6.29 KK136 pKa = 10.41 RR137 pKa = 11.84 YY138 pKa = 8.33 VACWNLLQCKK148 pKa = 9.45 SLAHH152 pKa = 5.78 EE153 pKa = 4.65 VPDD156 pKa = 5.05 EE157 pKa = 4.64 FIQDD161 pKa = 3.5 ALVKK165 pKa = 10.47 HH166 pKa = 6.28 RR167 pKa = 11.84 ATMEE171 pKa = 4.03 KK172 pKa = 10.07 EE173 pKa = 4.03 PEE175 pKa = 3.87 PLEE178 pKa = 4.36 IEE180 pKa = 4.11 VEE182 pKa = 3.66 EE183 pKa = 4.46 DD184 pKa = 2.83 FRR186 pKa = 11.84 HH187 pKa = 4.91 FVKK190 pKa = 10.69 PFVDD194 pKa = 3.81 QVIEE198 pKa = 4.07 QFDD201 pKa = 4.04 GKK203 pKa = 9.32 TSYY206 pKa = 7.91 PTNHH210 pKa = 5.97 SCVGFTRR217 pKa = 11.84 SGGGQLAAHH226 pKa = 6.25 SHH228 pKa = 7.22 RR229 pKa = 11.84 IRR231 pKa = 11.84 TSSGRR236 pKa = 11.84 RR237 pKa = 11.84 LPMGGRR243 pKa = 11.84 VGRR246 pKa = 11.84 KK247 pKa = 8.57 EE248 pKa = 3.91 PVVLTLEE255 pKa = 4.93 GPPSSGKK262 pKa = 8.56 STMVKK267 pKa = 10.38 YY268 pKa = 10.17 LVKK271 pKa = 10.08 EE272 pKa = 4.04 LQRR275 pKa = 11.84 QFQLKK280 pKa = 10.12 SDD282 pKa = 3.52 TVYY285 pKa = 10.79 YY286 pKa = 10.43 RR287 pKa = 11.84 SAATDD292 pKa = 2.66 HH293 pKa = 6.89 WDD295 pKa = 4.21 GYY297 pKa = 8.53 WGQPITVLDD306 pKa = 3.95 DD307 pKa = 3.41 VGAFASSMTQPSADD321 pKa = 3.78 LKK323 pKa = 11.11 EE324 pKa = 4.01 LLQLVSEE331 pKa = 4.82 CDD333 pKa = 3.61 YY334 pKa = 10.7 VLPMADD340 pKa = 4.5 LKK342 pKa = 11.5 DD343 pKa = 3.54 KK344 pKa = 10.77 GRR346 pKa = 11.84 KK347 pKa = 6.19 FTSPIIIMTTNYY359 pKa = 9.27 GARR362 pKa = 11.84 YY363 pKa = 7.68 NQTGFSCSAAIARR376 pKa = 11.84 RR377 pKa = 11.84 FGTVYY382 pKa = 10.32 RR383 pKa = 11.84 VVKK386 pKa = 9.57 GQAYY390 pKa = 7.62 DD391 pKa = 3.47 TYY393 pKa = 10.61 TQSTDD398 pKa = 3.24 QGCVDD403 pKa = 4.33 EE404 pKa = 5.05 MSHH407 pKa = 6.67 PLDD410 pKa = 3.56 SFVRR414 pKa = 11.84 KK415 pKa = 8.32 MRR417 pKa = 11.84 KK418 pKa = 9.16 DD419 pKa = 3.22 RR420 pKa = 11.84 PVSIRR425 pKa = 11.84 AMVNRR430 pKa = 11.84 LRR432 pKa = 11.84 TQYY435 pKa = 11.56 DD436 pKa = 3.58 EE437 pKa = 3.99 FHH439 pKa = 6.66 GNLRR443 pKa = 11.84 QEE445 pKa = 4.37 VLGMGTSLVFKK456 pKa = 10.94 KK457 pKa = 10.51 EE458 pKa = 4.09 DD459 pKa = 3.52 LSSLINTCEE468 pKa = 3.75 VTAIPEE474 pKa = 4.07 PLKK477 pKa = 10.86 VRR479 pKa = 11.84 TITRR483 pKa = 11.84 PEE485 pKa = 4.05 PLTYY489 pKa = 10.52 ALKK492 pKa = 10.22 PIQRR496 pKa = 11.84 AMFEE500 pKa = 3.8 ALKK503 pKa = 10.1 RR504 pKa = 11.84 WKK506 pKa = 10.24 CFQPCWDD513 pKa = 4.5 PNYY516 pKa = 10.87 SLQEE520 pKa = 3.77 LLRR523 pKa = 11.84 PEE525 pKa = 4.32 EE526 pKa = 4.65 GEE528 pKa = 4.15 TLLSGDD534 pKa = 3.9 YY535 pKa = 10.58 TSATDD540 pKa = 3.89 DD541 pKa = 3.43 LHH543 pKa = 6.92 PRR545 pKa = 11.84 LSQIVGEE552 pKa = 4.45 MIADD556 pKa = 3.99 RR557 pKa = 11.84 LGDD560 pKa = 3.85 GFLSDD565 pKa = 4.67 LIRR568 pKa = 11.84 WEE570 pKa = 4.33 SGPHH574 pKa = 6.4 LISYY578 pKa = 9.08 PKK580 pKa = 10.45 DD581 pKa = 3.28 SGLAAVWQTNGQLMGSLLSFPILCLANAFTMTRR614 pKa = 11.84 ATGTTLDD621 pKa = 3.34 TVRR624 pKa = 11.84 AVFHH628 pKa = 7.0 GDD630 pKa = 3.54 DD631 pKa = 3.31 IAAVATEE638 pKa = 4.09 SQISKK643 pKa = 9.37 WEE645 pKa = 4.05 KK646 pKa = 8.19 EE647 pKa = 3.95 ASSVGLSLSLGKK659 pKa = 10.42 NYY661 pKa = 10.09 VSRR664 pKa = 11.84 HH665 pKa = 4.49 FVSIDD670 pKa = 3.16 SQLFLWEE677 pKa = 4.8 DD678 pKa = 3.87 GEE680 pKa = 4.55 LVRR683 pKa = 11.84 KK684 pKa = 8.12 VTGKK688 pKa = 9.74 FRR690 pKa = 11.84 LVNRR694 pKa = 11.84 EE695 pKa = 3.76 AGEE698 pKa = 4.4 SCFEE702 pKa = 3.67 QALRR706 pKa = 11.84 QGFNKK711 pKa = 9.9 QLIRR715 pKa = 11.84 TLCKK719 pKa = 10.14 SQLAKK724 pKa = 10.34 SLRR727 pKa = 11.84 SLEE730 pKa = 4.24 VAVEE734 pKa = 3.91 QGGLGIQTTRR744 pKa = 11.84 PLTLWEE750 pKa = 3.63 KK751 pKa = 10.52 CYY753 pKa = 11.05 YY754 pKa = 9.78 LNRR757 pKa = 11.84 YY758 pKa = 7.2 QARR761 pKa = 11.84 TSCKK765 pKa = 10.18 EE766 pKa = 3.49 MGEE769 pKa = 4.4 GIRR772 pKa = 11.84 VPKK775 pKa = 9.98 EE776 pKa = 3.05 LAVRR780 pKa = 11.84 LRR782 pKa = 11.84 LTSVKK787 pKa = 11.04 SMMLPEE793 pKa = 4.74 EE794 pKa = 4.52 KK795 pKa = 10.09 PSEE798 pKa = 4.22 KK799 pKa = 10.46 NVNKK803 pKa = 10.07 QVWRR807 pKa = 11.84 LMKK810 pKa = 10.46 KK811 pKa = 9.82 CLKK814 pKa = 10.22 NSKK817 pKa = 10.06 FYY819 pKa = 11.32 EE820 pKa = 4.16 KK821 pKa = 10.61 VQNLSCSRR829 pKa = 11.84 PLSTLLTSVVRR840 pKa = 11.84 CDD842 pKa = 3.71 YY843 pKa = 11.12 SPQEE847 pKa = 3.99 LSRR850 pKa = 11.84 VYY852 pKa = 10.8 QQMFPMKK859 pKa = 10.39
Molecular weight: 97.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.797
IPC2_protein 8.624
IPC_protein 8.478
Toseland 9.194
ProMoST 9.092
Dawson 9.516
Bjellqvist 9.37
Wikipedia 9.663
Rodwell 9.78
Grimsley 9.545
Solomon 9.545
Lehninger 9.516
Nozaki 9.575
DTASelect 9.253
Thurlkill 9.414
EMBOSS 9.692
Sillero 9.575
Patrickios 4.952
IPC_peptide 9.545
IPC2_peptide 8.141
IPC2.peptide.svr19 7.793
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
859
859
859
859.0
97.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.355 ± 0.0
2.678 ± 0.0
4.54 ± 0.0
6.286 ± 0.0
4.191 ± 0.0
5.588 ± 0.0
2.095 ± 0.0
3.958 ± 0.0
7.218 ± 0.0
10.012 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.678 ± 0.0
2.678 ± 0.0
5.006 ± 0.0
4.657 ± 0.0
7.567 ± 0.0
8.498 ± 0.0
6.286 ± 0.0
6.286 ± 0.0
1.513 ± 0.0
2.91 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here