Wilkie partiti-like virus 1
Average proteome isoelectric point is 8.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTA3|A0A1Z2RTA3_9VIRU RdRp OS=Wilkie partiti-like virus 1 OX=2010283 PE=4 SV=1
MM1 pKa = 7.71 TDD3 pKa = 4.12 FEE5 pKa = 4.88 FFKK8 pKa = 10.55 IDD10 pKa = 3.47 NDD12 pKa = 4.43 RR13 pKa = 11.84 KK14 pKa = 10.55 FEE16 pKa = 4.66 DD17 pKa = 3.24 NRR19 pKa = 11.84 IPKK22 pKa = 9.0 TGIKK26 pKa = 10.26 GYY28 pKa = 8.15 PQYY31 pKa = 10.98 VYY33 pKa = 10.64 HH34 pKa = 6.54 VNQSFLQTNLEE45 pKa = 3.99 QSQFGPKK52 pKa = 9.86 PMTGVEE58 pKa = 4.08 EE59 pKa = 4.57 IIRR62 pKa = 11.84 GEE64 pKa = 3.85 FMEE67 pKa = 4.47 YY68 pKa = 10.62 TNVLYY73 pKa = 9.02 TYY75 pKa = 9.09 CRR77 pKa = 11.84 PKK79 pKa = 10.95 ADD81 pKa = 3.0 VDD83 pKa = 4.17 AVFNDD88 pKa = 3.87 FNKK91 pKa = 8.08 QTKK94 pKa = 9.01 PIKK97 pKa = 10.59 NLDD100 pKa = 3.6 RR101 pKa = 11.84 DD102 pKa = 3.76 TCEE105 pKa = 3.91 ATFLAIDD112 pKa = 4.23 RR113 pKa = 11.84 FMEE116 pKa = 3.98 IQPYY120 pKa = 10.42 DD121 pKa = 3.38 IVHH124 pKa = 5.85 WCDD127 pKa = 2.76 TRR129 pKa = 11.84 FYY131 pKa = 10.62 KK132 pKa = 10.35 WNLATKK138 pKa = 8.6 VDD140 pKa = 4.57 YY141 pKa = 10.9 YY142 pKa = 10.84 HH143 pKa = 6.62 SHH145 pKa = 5.57 SKK147 pKa = 8.65 TRR149 pKa = 11.84 LAEE152 pKa = 4.07 AKK154 pKa = 10.12 VNHH157 pKa = 6.67 PEE159 pKa = 4.06 EE160 pKa = 4.61 AKK162 pKa = 10.88 SPSKK166 pKa = 10.66 KK167 pKa = 9.07 HH168 pKa = 4.95 WFINTHH174 pKa = 6.33 LYY176 pKa = 10.11 RR177 pKa = 11.84 DD178 pKa = 3.4 RR179 pKa = 11.84 TVCHH183 pKa = 6.35 NIKK186 pKa = 10.46 LYY188 pKa = 10.61 GYY190 pKa = 8.58 PFVPTSGKK198 pKa = 9.36 NANLEE203 pKa = 3.99 LHH205 pKa = 6.16 KK206 pKa = 10.38 WFLKK210 pKa = 10.63 HH211 pKa = 5.02 PTEE214 pKa = 4.11 MLVRR218 pKa = 11.84 SHH220 pKa = 6.43 ISKK223 pKa = 10.44 RR224 pKa = 11.84 EE225 pKa = 3.83 KK226 pKa = 10.49 LKK228 pKa = 10.73 VRR230 pKa = 11.84 PVYY233 pKa = 10.02 CAPLLLLRR241 pKa = 11.84 LEE243 pKa = 5.66 CMLFWPLLAQCRR255 pKa = 11.84 KK256 pKa = 9.97 EE257 pKa = 4.38 EE258 pKa = 3.88 NCIMYY263 pKa = 10.44 GLEE266 pKa = 4.22 TIRR269 pKa = 11.84 GGMDD273 pKa = 3.6 YY274 pKa = 10.77 INRR277 pKa = 11.84 TATEE281 pKa = 3.92 YY282 pKa = 10.64 QRR284 pKa = 11.84 YY285 pKa = 9.64 LMLDD289 pKa = 3.37 WSSFDD294 pKa = 3.12 QLAPFKK300 pKa = 11.2 VIDD303 pKa = 3.99 LFFDD307 pKa = 3.09 EE308 pKa = 4.46 WLPRR312 pKa = 11.84 KK313 pKa = 9.73 ILVDD317 pKa = 3.32 NGYY320 pKa = 10.88 ALIWNYY326 pKa = 8.18 QAHH329 pKa = 4.94 VHH331 pKa = 6.06 NFQQEE336 pKa = 4.39 SKK338 pKa = 10.94 AHH340 pKa = 5.57 GTQSSPQPEE349 pKa = 4.25 NQEE352 pKa = 3.96 IPDD355 pKa = 4.1 LFPNKK360 pKa = 9.19 VRR362 pKa = 11.84 NLISFVKK369 pKa = 9.88 KK370 pKa = 9.16 WYY372 pKa = 10.29 KK373 pKa = 11.19 DD374 pKa = 3.48 MIFITPDD381 pKa = 2.59 GNAYY385 pKa = 10.06 RR386 pKa = 11.84 RR387 pKa = 11.84 INAGVPSGILMTQFLDD403 pKa = 3.09 SWINLFVFSYY413 pKa = 10.56 IQLKK417 pKa = 10.46 SGWTMKK423 pKa = 10.06 TLLANHH429 pKa = 6.92 IFVMGDD435 pKa = 3.24 DD436 pKa = 3.92 NVIFLPTGKK445 pKa = 9.74 RR446 pKa = 11.84 SHH448 pKa = 5.93 EE449 pKa = 3.88 QLLNSITNYY458 pKa = 10.04 AEE460 pKa = 4.76 KK461 pKa = 10.52 IFGMKK466 pKa = 10.33 LNQEE470 pKa = 4.13 KK471 pKa = 8.97 STFTANKK478 pKa = 10.01 NKK480 pKa = 10.02 IEE482 pKa = 4.28 VIGYY486 pKa = 5.57 TNKK489 pKa = 10.41 NGLPHH494 pKa = 7.21 RR495 pKa = 11.84 DD496 pKa = 2.91 IGKK499 pKa = 9.6 LVAQLAYY506 pKa = 9.69 PEE508 pKa = 4.33 RR509 pKa = 11.84 HH510 pKa = 6.58 CNDD513 pKa = 3.22 EE514 pKa = 4.24 DD515 pKa = 3.43 MCMRR519 pKa = 11.84 AVGMAYY525 pKa = 10.2 ASCGVNTTFYY535 pKa = 9.0 MFCKK539 pKa = 10.04 RR540 pKa = 11.84 VYY542 pKa = 10.11 DD543 pKa = 3.83 HH544 pKa = 6.8 YY545 pKa = 10.96 RR546 pKa = 11.84 SKK548 pKa = 11.31 LPLEE552 pKa = 3.89 YY553 pKa = 10.4 QIPFKK558 pKa = 10.4 RR559 pKa = 11.84 KK560 pKa = 8.25 NVIGIFKK567 pKa = 10.44 ALPEE571 pKa = 4.16 EE572 pKa = 4.35 VLSEE576 pKa = 4.19 FNFVEE581 pKa = 5.24 FPTLNEE587 pKa = 3.67 IFCRR591 pKa = 11.84 VQTHH595 pKa = 5.6 QGFLDD600 pKa = 3.56 IYY602 pKa = 10.09 KK603 pKa = 9.99 FWRR606 pKa = 11.84 SDD608 pKa = 3.42 YY609 pKa = 10.44 FLQPPVPKK617 pKa = 9.73 RR618 pKa = 11.84 RR619 pKa = 11.84 KK620 pKa = 8.99 RR621 pKa = 11.84 VTLHH625 pKa = 5.89 EE626 pKa = 4.35 YY627 pKa = 10.19 YY628 pKa = 10.22 RR629 pKa = 11.84 SKK631 pKa = 10.63 VWSTVTGFNRR641 pKa = 11.84 QADD644 pKa = 4.15 FRR646 pKa = 11.84 RR647 pKa = 11.84 LEE649 pKa = 4.04 SS650 pKa = 3.32
Molecular weight: 77.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.375
IPC2_protein 8.361
IPC_protein 8.2
Toseland 8.609
ProMoST 8.726
Dawson 9.077
Bjellqvist 9.077
Wikipedia 9.224
Rodwell 9.194
Grimsley 8.99
Solomon 9.136
Lehninger 9.092
Nozaki 9.151
DTASelect 8.931
Thurlkill 9.004
EMBOSS 9.209
Sillero 9.209
Patrickios 4.317
IPC_peptide 9.121
IPC2_peptide 7.819
IPC2.peptide.svr19 7.699
Protein with the highest isoelectric point:
>tr|A0A1Z2RTA3|A0A1Z2RTA3_9VIRU RdRp OS=Wilkie partiti-like virus 1 OX=2010283 PE=4 SV=1
MM1 pKa = 7.71 TDD3 pKa = 4.12 FEE5 pKa = 4.88 FFKK8 pKa = 10.55 IDD10 pKa = 3.47 NDD12 pKa = 4.43 RR13 pKa = 11.84 KK14 pKa = 10.55 FEE16 pKa = 4.66 DD17 pKa = 3.24 NRR19 pKa = 11.84 IPKK22 pKa = 9.0 TGIKK26 pKa = 10.26 GYY28 pKa = 8.15 PQYY31 pKa = 10.98 VYY33 pKa = 10.64 HH34 pKa = 6.54 VNQSFLQTNLEE45 pKa = 3.99 QSQFGPKK52 pKa = 9.86 PMTGVEE58 pKa = 4.08 EE59 pKa = 4.57 IIRR62 pKa = 11.84 GEE64 pKa = 3.85 FMEE67 pKa = 4.47 YY68 pKa = 10.62 TNVLYY73 pKa = 9.02 TYY75 pKa = 9.09 CRR77 pKa = 11.84 PKK79 pKa = 10.95 ADD81 pKa = 3.0 VDD83 pKa = 4.17 AVFNDD88 pKa = 3.87 FNKK91 pKa = 8.08 QTKK94 pKa = 9.01 PIKK97 pKa = 10.59 NLDD100 pKa = 3.6 RR101 pKa = 11.84 DD102 pKa = 3.76 TCEE105 pKa = 3.91 ATFLAIDD112 pKa = 4.23 RR113 pKa = 11.84 FMEE116 pKa = 3.98 IQPYY120 pKa = 10.42 DD121 pKa = 3.38 IVHH124 pKa = 5.85 WCDD127 pKa = 2.76 TRR129 pKa = 11.84 FYY131 pKa = 10.62 KK132 pKa = 10.35 WNLATKK138 pKa = 8.6 VDD140 pKa = 4.57 YY141 pKa = 10.9 YY142 pKa = 10.84 HH143 pKa = 6.62 SHH145 pKa = 5.57 SKK147 pKa = 8.65 TRR149 pKa = 11.84 LAEE152 pKa = 4.07 AKK154 pKa = 10.12 VNHH157 pKa = 6.67 PEE159 pKa = 4.06 EE160 pKa = 4.61 AKK162 pKa = 10.88 SPSKK166 pKa = 10.66 KK167 pKa = 9.07 HH168 pKa = 4.95 WFINTHH174 pKa = 6.33 LYY176 pKa = 10.11 RR177 pKa = 11.84 DD178 pKa = 3.4 RR179 pKa = 11.84 TVCHH183 pKa = 6.35 NIKK186 pKa = 10.46 LYY188 pKa = 10.61 GYY190 pKa = 8.58 PFVPTSGKK198 pKa = 9.36 NANLEE203 pKa = 3.99 LHH205 pKa = 6.16 KK206 pKa = 10.38 WFLKK210 pKa = 10.63 HH211 pKa = 5.02 PTEE214 pKa = 4.11 MLVRR218 pKa = 11.84 SHH220 pKa = 6.43 ISKK223 pKa = 10.44 RR224 pKa = 11.84 EE225 pKa = 3.83 KK226 pKa = 10.49 LKK228 pKa = 10.73 VRR230 pKa = 11.84 PVYY233 pKa = 10.02 CAPLLLLRR241 pKa = 11.84 LEE243 pKa = 5.66 CMLFWPLLAQCRR255 pKa = 11.84 KK256 pKa = 9.97 EE257 pKa = 4.38 EE258 pKa = 3.88 NCIMYY263 pKa = 10.44 GLEE266 pKa = 4.22 TIRR269 pKa = 11.84 GGMDD273 pKa = 3.6 YY274 pKa = 10.77 INRR277 pKa = 11.84 TATEE281 pKa = 3.92 YY282 pKa = 10.64 QRR284 pKa = 11.84 YY285 pKa = 9.64 LMLDD289 pKa = 3.37 WSSFDD294 pKa = 3.12 QLAPFKK300 pKa = 11.2 VIDD303 pKa = 3.99 LFFDD307 pKa = 3.09 EE308 pKa = 4.46 WLPRR312 pKa = 11.84 KK313 pKa = 9.73 ILVDD317 pKa = 3.32 NGYY320 pKa = 10.88 ALIWNYY326 pKa = 8.18 QAHH329 pKa = 4.94 VHH331 pKa = 6.06 NFQQEE336 pKa = 4.39 SKK338 pKa = 10.94 AHH340 pKa = 5.57 GTQSSPQPEE349 pKa = 4.25 NQEE352 pKa = 3.96 IPDD355 pKa = 4.1 LFPNKK360 pKa = 9.19 VRR362 pKa = 11.84 NLISFVKK369 pKa = 9.88 KK370 pKa = 9.16 WYY372 pKa = 10.29 KK373 pKa = 11.19 DD374 pKa = 3.48 MIFITPDD381 pKa = 2.59 GNAYY385 pKa = 10.06 RR386 pKa = 11.84 RR387 pKa = 11.84 INAGVPSGILMTQFLDD403 pKa = 3.09 SWINLFVFSYY413 pKa = 10.56 IQLKK417 pKa = 10.46 SGWTMKK423 pKa = 10.06 TLLANHH429 pKa = 6.92 IFVMGDD435 pKa = 3.24 DD436 pKa = 3.92 NVIFLPTGKK445 pKa = 9.74 RR446 pKa = 11.84 SHH448 pKa = 5.93 EE449 pKa = 3.88 QLLNSITNYY458 pKa = 10.04 AEE460 pKa = 4.76 KK461 pKa = 10.52 IFGMKK466 pKa = 10.33 LNQEE470 pKa = 4.13 KK471 pKa = 8.97 STFTANKK478 pKa = 10.01 NKK480 pKa = 10.02 IEE482 pKa = 4.28 VIGYY486 pKa = 5.57 TNKK489 pKa = 10.41 NGLPHH494 pKa = 7.21 RR495 pKa = 11.84 DD496 pKa = 2.91 IGKK499 pKa = 9.6 LVAQLAYY506 pKa = 9.69 PEE508 pKa = 4.33 RR509 pKa = 11.84 HH510 pKa = 6.58 CNDD513 pKa = 3.22 EE514 pKa = 4.24 DD515 pKa = 3.43 MCMRR519 pKa = 11.84 AVGMAYY525 pKa = 10.2 ASCGVNTTFYY535 pKa = 9.0 MFCKK539 pKa = 10.04 RR540 pKa = 11.84 VYY542 pKa = 10.11 DD543 pKa = 3.83 HH544 pKa = 6.8 YY545 pKa = 10.96 RR546 pKa = 11.84 SKK548 pKa = 11.31 LPLEE552 pKa = 3.89 YY553 pKa = 10.4 QIPFKK558 pKa = 10.4 RR559 pKa = 11.84 KK560 pKa = 8.25 NVIGIFKK567 pKa = 10.44 ALPEE571 pKa = 4.16 EE572 pKa = 4.35 VLSEE576 pKa = 4.19 FNFVEE581 pKa = 5.24 FPTLNEE587 pKa = 3.67 IFCRR591 pKa = 11.84 VQTHH595 pKa = 5.6 QGFLDD600 pKa = 3.56 IYY602 pKa = 10.09 KK603 pKa = 9.99 FWRR606 pKa = 11.84 SDD608 pKa = 3.42 YY609 pKa = 10.44 FLQPPVPKK617 pKa = 9.73 RR618 pKa = 11.84 RR619 pKa = 11.84 KK620 pKa = 8.99 RR621 pKa = 11.84 VTLHH625 pKa = 5.89 EE626 pKa = 4.35 YY627 pKa = 10.19 YY628 pKa = 10.22 RR629 pKa = 11.84 SKK631 pKa = 10.63 VWSTVTGFNRR641 pKa = 11.84 QADD644 pKa = 4.15 FRR646 pKa = 11.84 RR647 pKa = 11.84 LEE649 pKa = 4.04 SS650 pKa = 3.32
Molecular weight: 77.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.375
IPC2_protein 8.361
IPC_protein 8.2
Toseland 8.609
ProMoST 8.726
Dawson 9.077
Bjellqvist 9.077
Wikipedia 9.224
Rodwell 9.194
Grimsley 8.99
Solomon 9.136
Lehninger 9.092
Nozaki 9.151
DTASelect 8.931
Thurlkill 9.004
EMBOSS 9.209
Sillero 9.209
Patrickios 4.317
IPC_peptide 9.121
IPC2_peptide 7.819
IPC2.peptide.svr19 7.699
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
650
650
650
650.0
77.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.308 ± 0.0
2.0 ± 0.0
5.077 ± 0.0
5.846 ± 0.0
6.769 ± 0.0
4.154 ± 0.0
3.231 ± 0.0
5.846 ± 0.0
7.692 ± 0.0
8.308 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.769 ± 0.0
6.154 ± 0.0
5.077 ± 0.0
4.0 ± 0.0
5.846 ± 0.0
4.462 ± 0.0
5.538 ± 0.0
5.538 ± 0.0
2.0 ± 0.0
5.385 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here