Pseudomonas phage ZC08

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Zicotriavirus; Pseudomonas virus ZC08

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2C9A1|A0A1L2C9A1_9CAUD Uncharacterized protein OS=Pseudomonas phage ZC08 OX=1622116 GN=ZC08_085 PE=4 SV=1
MM1 pKa = 7.68INTSLFKK8 pKa = 10.52NAHH11 pKa = 5.46AVMKK15 pKa = 9.67TVEE18 pKa = 4.27EE19 pKa = 4.04LSDD22 pKa = 3.4YY23 pKa = 10.98KK24 pKa = 10.64FAYY27 pKa = 10.36RR28 pKa = 11.84NGNDD32 pKa = 3.21TLFFSIGGFDD42 pKa = 3.36VSAVYY47 pKa = 10.37EE48 pKa = 4.13NYY50 pKa = 10.42SIEE53 pKa = 4.28LYY55 pKa = 10.7EE56 pKa = 5.19VDD58 pKa = 4.77LQGNLIKK65 pKa = 10.72DD66 pKa = 3.64GEE68 pKa = 4.53YY69 pKa = 10.31IEE71 pKa = 5.59VDD73 pKa = 3.87IPDD76 pKa = 3.69TDD78 pKa = 3.8ADD80 pKa = 3.94AVLTTLLKK88 pKa = 10.49HH89 pKa = 5.84GLVPVVMVPEE99 pKa = 4.49ASSYY103 pKa = 5.98TTEE106 pKa = 4.3PEE108 pKa = 3.91EE109 pKa = 4.18EE110 pKa = 4.21EE111 pKa = 4.56PEE113 pKa = 3.98EE114 pKa = 5.47DD115 pKa = 4.45EE116 pKa = 4.83QKK118 pKa = 10.81CDD120 pKa = 4.2CSLCSAQDD128 pKa = 3.78SEE130 pKa = 4.78SLSALFDD137 pKa = 5.19LIFVLNKK144 pKa = 10.26DD145 pKa = 3.97FKK147 pKa = 11.07NN148 pKa = 3.58

Molecular weight: 16.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2C9D5|A0A1L2C9D5_9CAUD Uncharacterized protein OS=Pseudomonas phage ZC08 OX=1622116 GN=ZC08_045 PE=4 SV=1
MM1 pKa = 7.71INTTLLVTRR10 pKa = 11.84LNARR14 pKa = 11.84CLQIVAEE21 pKa = 4.29EE22 pKa = 4.43SGVSYY27 pKa = 11.12SCLSNIRR34 pKa = 11.84HH35 pKa = 5.98GRR37 pKa = 11.84TKK39 pKa = 10.71LPQHH43 pKa = 5.65RR44 pKa = 11.84TLLKK48 pKa = 10.19LLPVLGLKK56 pKa = 10.61LEE58 pKa = 4.44IKK60 pKa = 10.69NDD62 pKa = 3.41

Molecular weight: 6.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

21760

47

3677

262.2

29.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.155 ± 0.429

0.804 ± 0.191

5.933 ± 0.176

7.817 ± 0.281

3.883 ± 0.181

6.006 ± 0.267

1.811 ± 0.162

6.402 ± 0.182

6.379 ± 0.347

8.911 ± 0.19

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.449 ± 0.125

5.8 ± 0.208

4.265 ± 0.178

3.897 ± 0.168

5.005 ± 0.172

6.613 ± 0.28

5.689 ± 0.188

6.388 ± 0.18

1.126 ± 0.136

3.649 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski