Honeysuckle yellow vein virus-[Japan:Masuda:2003]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Honeysuckle yellow vein virus

Average proteome isoelectric point is 7.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4F1W4|A4F1W4_9GEMI C4 OS=Honeysuckle yellow vein virus-[Japan:Masuda:2003] OX=435489 GN=C4 PE=3 SV=1
MM1 pKa = 7.7APPKK5 pKa = 10.16RR6 pKa = 11.84FKK8 pKa = 10.74INSKK12 pKa = 10.72NYY14 pKa = 8.38FLTYY18 pKa = 7.96PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.09EE27 pKa = 4.94ALSQIQALEE36 pKa = 4.14TPTNKK41 pKa = 10.07LFIRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.54LHH51 pKa = 6.34EE52 pKa = 5.86DD53 pKa = 4.22GSPHH57 pKa = 6.11LHH59 pKa = 6.16VLIQFEE65 pKa = 4.74GKK67 pKa = 8.8YY68 pKa = 9.53QCTNQRR74 pKa = 11.84FFDD77 pKa = 3.98LVSPSRR83 pKa = 11.84SAHH86 pKa = 4.9FHH88 pKa = 6.45PNIQGAKK95 pKa = 9.45SSSDD99 pKa = 2.96VKK101 pKa = 11.2AYY103 pKa = 8.93MEE105 pKa = 4.62KK106 pKa = 10.75DD107 pKa = 2.91GDD109 pKa = 4.06VIDD112 pKa = 4.8FGVFQVDD119 pKa = 3.16GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.37SSALSILRR151 pKa = 11.84EE152 pKa = 3.92KK153 pKa = 10.75APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.05RR171 pKa = 11.84IFAPPLEE178 pKa = 4.52EE179 pKa = 3.86FVSPFSSSSFDD190 pKa = 3.44QVPDD194 pKa = 3.78EE195 pKa = 4.36LEE197 pKa = 3.49EE198 pKa = 3.89WAAEE202 pKa = 4.05NVRR205 pKa = 11.84DD206 pKa = 3.83SAARR210 pKa = 11.84PWRR213 pKa = 11.84PMSIVIEE220 pKa = 4.37GDD222 pKa = 3.13SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLSPRR237 pKa = 11.84HH238 pKa = 6.09NYY240 pKa = 10.09LCGHH244 pKa = 7.41LDD246 pKa = 4.18LSPKK250 pKa = 10.02VYY252 pKa = 10.89SNEE255 pKa = 3.02AWYY258 pKa = 10.82NVIDD262 pKa = 5.29DD263 pKa = 4.41VDD265 pKa = 3.61PHH267 pKa = 5.85YY268 pKa = 11.0LKK270 pKa = 10.7HH271 pKa = 6.2FKK273 pKa = 10.7EE274 pKa = 4.46FMGAQRR280 pKa = 11.84DD281 pKa = 3.81WQSNTKK287 pKa = 9.26YY288 pKa = 10.63GKK290 pKa = 9.49PIQIKK295 pKa = 10.42GGIPTIFLCNPGPTSSYY312 pKa = 10.65TEE314 pKa = 3.99YY315 pKa = 11.22LDD317 pKa = 3.75EE318 pKa = 4.92EE319 pKa = 4.46KK320 pKa = 11.02NAALKK325 pKa = 10.42AWAIKK330 pKa = 10.27NATFVTLYY338 pKa = 10.64GPLYY342 pKa = 10.67SGTHH346 pKa = 5.0QGATQIRR353 pKa = 11.84EE354 pKa = 4.21EE355 pKa = 4.35EE356 pKa = 4.3STSPEE361 pKa = 4.01EE362 pKa = 3.99NN363 pKa = 3.08

Molecular weight: 40.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4F1W1|A4F1W1_9GEMI Replication enhancer OS=Honeysuckle yellow vein virus-[Japan:Masuda:2003] OX=435489 GN=C3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.31RR4 pKa = 11.84PADD7 pKa = 3.24IGIFTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.18SQSANRR30 pKa = 11.84ASVPIALATNKK41 pKa = 9.29KK42 pKa = 9.97RR43 pKa = 11.84SWAQRR48 pKa = 11.84PMYY51 pKa = 9.7RR52 pKa = 11.84KK53 pKa = 8.22PRR55 pKa = 11.84MYY57 pKa = 10.82RR58 pKa = 11.84MYY60 pKa = 10.44RR61 pKa = 11.84SPDD64 pKa = 3.21VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.21VQSYY78 pKa = 7.95EE79 pKa = 3.85KK80 pKa = 10.56KK81 pKa = 10.39NDD83 pKa = 3.44VGHH86 pKa = 6.71SGTLLCVSDD95 pKa = 3.33ITRR98 pKa = 11.84GNGLTHH104 pKa = 6.89RR105 pKa = 11.84VGKK108 pKa = 9.82RR109 pKa = 11.84FCIKK113 pKa = 9.93SVYY116 pKa = 10.03IIGKK120 pKa = 8.43IWMDD124 pKa = 3.48EE125 pKa = 4.05NIKK128 pKa = 9.34TKK130 pKa = 10.62NHH132 pKa = 5.4TNNVLFWLVRR142 pKa = 11.84DD143 pKa = 4.16RR144 pKa = 11.84RR145 pKa = 11.84PGSTPYY151 pKa = 10.86GFQEE155 pKa = 4.07AFNMFEE161 pKa = 4.62NEE163 pKa = 3.81PSTATVKK170 pKa = 10.75QEE172 pKa = 3.83LRR174 pKa = 11.84DD175 pKa = 3.75RR176 pKa = 11.84LQVLHH181 pKa = 6.91RR182 pKa = 11.84FSATVTGGQYY192 pKa = 10.78ASKK195 pKa = 9.07EE196 pKa = 3.69QAVIKK201 pKa = 10.06RR202 pKa = 11.84FWKK205 pKa = 10.24LNHH208 pKa = 6.28HH209 pKa = 5.5VTYY212 pKa = 10.93NHH214 pKa = 6.12QEE216 pKa = 3.62QAKK219 pKa = 9.66YY220 pKa = 10.54EE221 pKa = 4.12NHH223 pKa = 6.33TEE225 pKa = 3.99NALLLYY231 pKa = 7.29MACTHH236 pKa = 7.07ASNPVYY242 pKa = 9.77ATLKK246 pKa = 9.27IRR248 pKa = 11.84VYY250 pKa = 10.64FYY252 pKa = 11.38DD253 pKa = 3.47SVQNN257 pKa = 3.87

Molecular weight: 29.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1102

97

363

183.7

21.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.989 ± 0.57

2.087 ± 0.376

4.9 ± 0.447

5.172 ± 0.97

4.446 ± 0.458

4.809 ± 0.532

4.265 ± 0.548

5.263 ± 0.5

5.354 ± 0.694

7.35 ± 0.643

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.996 ± 0.353

5.263 ± 0.494

5.535 ± 0.544

5.445 ± 0.647

6.624 ± 0.866

8.53 ± 1.561

6.171 ± 0.806

5.535 ± 0.972

1.361 ± 0.144

3.902 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski