Amino acid dipepetide frequency for Streptococcus phage Javan231

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.874AlaAla: 3.874 ± 1.366
0.44AlaCys: 0.44 ± 0.188
4.578AlaAsp: 4.578 ± 0.563
5.546AlaGlu: 5.546 ± 0.871
2.025AlaPhe: 2.025 ± 0.384
4.578AlaGly: 4.578 ± 0.907
0.968AlaHis: 0.968 ± 0.275
5.81AlaIle: 5.81 ± 1.0
5.898AlaLys: 5.898 ± 0.713
5.458AlaLeu: 5.458 ± 0.745
1.937AlaMet: 1.937 ± 0.423
4.226AlaAsn: 4.226 ± 0.676
1.497AlaPro: 1.497 ± 0.445
3.345AlaGln: 3.345 ± 0.691
3.433AlaArg: 3.433 ± 0.643
5.546AlaSer: 5.546 ± 0.878
4.49AlaThr: 4.49 ± 0.592
5.106AlaVal: 5.106 ± 0.784
1.409AlaTrp: 1.409 ± 0.571
2.465AlaTyr: 2.465 ± 0.649
0.0AlaXaa: 0.0 ± 0.0
Cys
0.44CysAla: 0.44 ± 0.186
0.0CysCys: 0.0 ± 0.0
0.792CysAsp: 0.792 ± 0.295
0.176CysGlu: 0.176 ± 0.128
0.088CysPhe: 0.088 ± 0.084
0.616CysGly: 0.616 ± 0.284
0.176CysHis: 0.176 ± 0.101
0.176CysIle: 0.176 ± 0.107
0.352CysLys: 0.352 ± 0.158
0.264CysLeu: 0.264 ± 0.126
0.088CysMet: 0.088 ± 0.104
0.0CysAsn: 0.0 ± 0.0
0.176CysPro: 0.176 ± 0.121
0.264CysGln: 0.264 ± 0.193
0.176CysArg: 0.176 ± 0.121
0.616CysSer: 0.616 ± 0.203
0.264CysThr: 0.264 ± 0.161
0.352CysVal: 0.352 ± 0.165
0.176CysTrp: 0.176 ± 0.13
0.352CysTyr: 0.352 ± 0.215
0.0CysXaa: 0.0 ± 0.0
Asp
2.641AspAla: 2.641 ± 0.57
0.44AspCys: 0.44 ± 0.223
4.842AspAsp: 4.842 ± 0.753
6.074AspGlu: 6.074 ± 1.158
3.257AspPhe: 3.257 ± 0.637
4.314AspGly: 4.314 ± 0.963
0.528AspHis: 0.528 ± 0.238
3.433AspIle: 3.433 ± 0.489
3.962AspLys: 3.962 ± 0.547
6.074AspLeu: 6.074 ± 0.699
2.465AspMet: 2.465 ± 0.607
3.874AspAsn: 3.874 ± 0.655
1.144AspPro: 1.144 ± 0.338
1.321AspGln: 1.321 ± 0.356
1.761AspArg: 1.761 ± 0.35
3.786AspSer: 3.786 ± 0.632
4.314AspThr: 4.314 ± 0.524
3.962AspVal: 3.962 ± 0.76
1.321AspTrp: 1.321 ± 0.345
3.521AspTyr: 3.521 ± 0.614
0.0AspXaa: 0.0 ± 0.0
Glu
6.779GluAla: 6.779 ± 0.976
0.088GluCys: 0.088 ± 0.092
3.874GluAsp: 3.874 ± 0.8
4.666GluGlu: 4.666 ± 0.83
3.345GluPhe: 3.345 ± 0.542
3.257GluGly: 3.257 ± 0.651
0.968GluHis: 0.968 ± 0.277
6.427GluIle: 6.427 ± 0.844
5.018GluLys: 5.018 ± 0.8
8.716GluLeu: 8.716 ± 1.264
2.113GluMet: 2.113 ± 0.565
3.962GluAsn: 3.962 ± 0.671
2.201GluPro: 2.201 ± 0.419
2.641GluGln: 2.641 ± 0.586
2.289GluArg: 2.289 ± 0.435
3.169GluSer: 3.169 ± 0.574
4.49GluThr: 4.49 ± 0.541
5.018GluVal: 5.018 ± 0.723
0.88GluTrp: 0.88 ± 0.353
2.553GluTyr: 2.553 ± 0.421
0.0GluXaa: 0.0 ± 0.0
Phe
2.377PheAla: 2.377 ± 0.485
0.088PheCys: 0.088 ± 0.092
3.345PheAsp: 3.345 ± 0.453
4.402PheGlu: 4.402 ± 0.867
1.673PhePhe: 1.673 ± 0.421
2.553PheGly: 2.553 ± 0.425
0.44PheHis: 0.44 ± 0.24
2.377PheIle: 2.377 ± 0.453
3.521PheLys: 3.521 ± 0.496
2.817PheLeu: 2.817 ± 0.571
1.233PheMet: 1.233 ± 0.334
2.641PheAsn: 2.641 ± 0.612
1.321PhePro: 1.321 ± 0.35
0.528PheGln: 0.528 ± 0.237
1.144PheArg: 1.144 ± 0.284
2.993PheSer: 2.993 ± 0.424
2.025PheThr: 2.025 ± 0.328
2.465PheVal: 2.465 ± 0.542
0.528PheTrp: 0.528 ± 0.201
1.585PheTyr: 1.585 ± 0.408
0.0PheXaa: 0.0 ± 0.0
Gly
4.314GlyAla: 4.314 ± 1.118
0.44GlyCys: 0.44 ± 0.247
2.729GlyAsp: 2.729 ± 0.412
3.345GlyGlu: 3.345 ± 0.507
2.641GlyPhe: 2.641 ± 0.433
3.169GlyGly: 3.169 ± 0.742
0.968GlyHis: 0.968 ± 0.284
5.634GlyIle: 5.634 ± 0.999
4.754GlyLys: 4.754 ± 0.593
5.546GlyLeu: 5.546 ± 0.918
1.321GlyMet: 1.321 ± 0.371
4.05GlyAsn: 4.05 ± 0.647
0.44GlyPro: 0.44 ± 0.269
2.201GlyGln: 2.201 ± 0.462
2.025GlyArg: 2.025 ± 0.429
4.138GlySer: 4.138 ± 0.822
3.698GlyThr: 3.698 ± 0.706
3.609GlyVal: 3.609 ± 0.538
0.792GlyTrp: 0.792 ± 0.307
2.817GlyTyr: 2.817 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
0.704HisAla: 0.704 ± 0.308
0.176HisCys: 0.176 ± 0.163
0.44HisAsp: 0.44 ± 0.204
1.056HisGlu: 1.056 ± 0.376
0.968HisPhe: 0.968 ± 0.331
0.968HisGly: 0.968 ± 0.367
0.176HisHis: 0.176 ± 0.125
0.704HisIle: 0.704 ± 0.3
0.968HisLys: 0.968 ± 0.24
0.704HisLeu: 0.704 ± 0.255
0.352HisMet: 0.352 ± 0.231
0.352HisAsn: 0.352 ± 0.161
0.44HisPro: 0.44 ± 0.219
0.792HisGln: 0.792 ± 0.285
0.176HisArg: 0.176 ± 0.124
1.144HisSer: 1.144 ± 0.255
0.704HisThr: 0.704 ± 0.257
0.88HisVal: 0.88 ± 0.328
0.264HisTrp: 0.264 ± 0.164
0.528HisTyr: 0.528 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
6.691IleAla: 6.691 ± 0.93
0.616IleCys: 0.616 ± 0.194
4.314IleAsp: 4.314 ± 0.624
5.018IleGlu: 5.018 ± 0.908
2.289IlePhe: 2.289 ± 0.505
2.905IleGly: 2.905 ± 0.448
0.616IleHis: 0.616 ± 0.252
3.345IleIle: 3.345 ± 0.69
7.219IleLys: 7.219 ± 0.84
5.106IleLeu: 5.106 ± 0.727
1.585IleMet: 1.585 ± 0.375
3.962IleAsn: 3.962 ± 0.573
1.585IlePro: 1.585 ± 0.264
2.465IleGln: 2.465 ± 0.434
1.937IleArg: 1.937 ± 0.32
4.578IleSer: 4.578 ± 0.623
4.49IleThr: 4.49 ± 1.018
5.018IleVal: 5.018 ± 0.878
1.144IleTrp: 1.144 ± 0.393
2.817IleTyr: 2.817 ± 0.554
0.0IleXaa: 0.0 ± 0.0
Lys
6.074LysAla: 6.074 ± 0.745
0.352LysCys: 0.352 ± 0.173
4.93LysAsp: 4.93 ± 0.688
7.043LysGlu: 7.043 ± 0.948
2.993LysPhe: 2.993 ± 0.521
3.433LysGly: 3.433 ± 0.603
0.528LysHis: 0.528 ± 0.218
5.546LysIle: 5.546 ± 0.811
6.163LysLys: 6.163 ± 1.075
6.603LysLeu: 6.603 ± 0.826
1.761LysMet: 1.761 ± 0.484
6.074LysAsn: 6.074 ± 0.746
1.849LysPro: 1.849 ± 0.365
4.226LysGln: 4.226 ± 0.656
4.49LysArg: 4.49 ± 0.913
4.402LysSer: 4.402 ± 0.731
4.842LysThr: 4.842 ± 0.727
5.722LysVal: 5.722 ± 0.764
0.968LysTrp: 0.968 ± 0.347
3.521LysTyr: 3.521 ± 0.674
0.0LysXaa: 0.0 ± 0.0
Leu
6.251LeuAla: 6.251 ± 0.899
0.264LeuCys: 0.264 ± 0.173
5.634LeuAsp: 5.634 ± 0.729
6.427LeuGlu: 6.427 ± 1.224
2.377LeuPhe: 2.377 ± 0.51
5.106LeuGly: 5.106 ± 0.868
1.409LeuHis: 1.409 ± 0.397
4.754LeuIle: 4.754 ± 0.798
8.539LeuLys: 8.539 ± 0.822
5.634LeuLeu: 5.634 ± 0.894
1.585LeuMet: 1.585 ± 0.393
5.81LeuAsn: 5.81 ± 1.146
2.553LeuPro: 2.553 ± 0.506
2.817LeuGln: 2.817 ± 0.609
2.993LeuArg: 2.993 ± 0.509
6.251LeuSer: 6.251 ± 0.778
4.314LeuThr: 4.314 ± 0.749
4.666LeuVal: 4.666 ± 0.597
0.616LeuTrp: 0.616 ± 0.335
2.377LeuTyr: 2.377 ± 0.533
0.0LeuXaa: 0.0 ± 0.0
Met
2.465MetAla: 2.465 ± 0.551
0.088MetCys: 0.088 ± 0.085
1.233MetAsp: 1.233 ± 0.434
1.144MetGlu: 1.144 ± 0.311
0.88MetPhe: 0.88 ± 0.294
0.704MetGly: 0.704 ± 0.338
0.704MetHis: 0.704 ± 0.345
1.056MetIle: 1.056 ± 0.309
2.201MetLys: 2.201 ± 0.433
1.497MetLeu: 1.497 ± 0.286
0.616MetMet: 0.616 ± 0.261
1.409MetAsn: 1.409 ± 0.288
0.88MetPro: 0.88 ± 0.304
1.585MetGln: 1.585 ± 0.385
1.056MetArg: 1.056 ± 0.368
1.409MetSer: 1.409 ± 0.349
2.729MetThr: 2.729 ± 0.545
0.704MetVal: 0.704 ± 0.201
0.176MetTrp: 0.176 ± 0.109
0.88MetTyr: 0.88 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
3.345AsnAla: 3.345 ± 0.633
0.264AsnCys: 0.264 ± 0.121
2.553AsnAsp: 2.553 ± 0.5
2.729AsnGlu: 2.729 ± 0.516
2.201AsnPhe: 2.201 ± 0.369
6.339AsnGly: 6.339 ± 0.596
0.968AsnHis: 0.968 ± 0.362
3.345AsnIle: 3.345 ± 0.78
5.018AsnLys: 5.018 ± 0.867
4.754AsnLeu: 4.754 ± 0.793
0.704AsnMet: 0.704 ± 0.27
4.138AsnAsn: 4.138 ± 0.906
2.377AsnPro: 2.377 ± 0.453
2.993AsnGln: 2.993 ± 0.544
2.641AsnArg: 2.641 ± 0.509
3.962AsnSer: 3.962 ± 0.878
4.666AsnThr: 4.666 ± 1.091
3.874AsnVal: 3.874 ± 0.76
1.233AsnTrp: 1.233 ± 0.325
2.905AsnTyr: 2.905 ± 0.575
0.0AsnXaa: 0.0 ± 0.0
Pro
2.113ProAla: 2.113 ± 0.376
0.088ProCys: 0.088 ± 0.084
1.937ProAsp: 1.937 ± 0.386
2.201ProGlu: 2.201 ± 0.434
0.88ProPhe: 0.88 ± 0.287
0.616ProGly: 0.616 ± 0.271
0.44ProHis: 0.44 ± 0.193
1.937ProIle: 1.937 ± 0.407
2.025ProLys: 2.025 ± 0.556
1.321ProLeu: 1.321 ± 0.3
0.44ProMet: 0.44 ± 0.171
2.025ProAsn: 2.025 ± 0.653
0.352ProPro: 0.352 ± 0.178
0.968ProGln: 0.968 ± 0.32
1.409ProArg: 1.409 ± 0.493
2.025ProSer: 2.025 ± 0.317
2.025ProThr: 2.025 ± 0.392
1.761ProVal: 1.761 ± 0.288
0.264ProTrp: 0.264 ± 0.115
1.233ProTyr: 1.233 ± 0.35
0.0ProXaa: 0.0 ± 0.0
Gln
3.698GlnAla: 3.698 ± 0.655
0.176GlnCys: 0.176 ± 0.13
1.761GlnAsp: 1.761 ± 0.385
3.345GlnGlu: 3.345 ± 0.823
1.585GlnPhe: 1.585 ± 0.428
2.025GlnGly: 2.025 ± 0.452
0.528GlnHis: 0.528 ± 0.251
2.905GlnIle: 2.905 ± 0.631
3.433GlnLys: 3.433 ± 0.704
2.905GlnLeu: 2.905 ± 0.469
0.352GlnMet: 0.352 ± 0.186
2.289GlnAsn: 2.289 ± 0.421
1.056GlnPro: 1.056 ± 0.385
2.025GlnGln: 2.025 ± 0.522
1.761GlnArg: 1.761 ± 0.406
2.289GlnSer: 2.289 ± 0.409
3.521GlnThr: 3.521 ± 0.686
3.345GlnVal: 3.345 ± 0.96
0.176GlnTrp: 0.176 ± 0.134
1.497GlnTyr: 1.497 ± 0.411
0.0GlnXaa: 0.0 ± 0.0
Arg
3.521ArgAla: 3.521 ± 0.77
0.264ArgCys: 0.264 ± 0.139
1.761ArgAsp: 1.761 ± 0.355
2.553ArgGlu: 2.553 ± 0.564
1.849ArgPhe: 1.849 ± 0.391
1.144ArgGly: 1.144 ± 0.359
0.176ArgHis: 0.176 ± 0.099
2.729ArgIle: 2.729 ± 0.549
3.962ArgLys: 3.962 ± 0.863
2.817ArgLeu: 2.817 ± 0.483
1.497ArgMet: 1.497 ± 0.275
1.585ArgAsn: 1.585 ± 0.373
0.792ArgPro: 0.792 ± 0.208
2.465ArgGln: 2.465 ± 0.532
1.321ArgArg: 1.321 ± 0.523
1.673ArgSer: 1.673 ± 0.457
1.937ArgThr: 1.937 ± 0.354
2.641ArgVal: 2.641 ± 0.552
0.176ArgTrp: 0.176 ± 0.155
2.553ArgTyr: 2.553 ± 0.518
0.0ArgXaa: 0.0 ± 0.0
Ser
4.754SerAla: 4.754 ± 0.786
0.176SerCys: 0.176 ± 0.137
4.754SerAsp: 4.754 ± 0.627
5.106SerGlu: 5.106 ± 0.633
3.345SerPhe: 3.345 ± 0.449
4.93SerGly: 4.93 ± 0.639
0.704SerHis: 0.704 ± 0.216
5.018SerIle: 5.018 ± 0.74
4.666SerLys: 4.666 ± 0.756
5.194SerLeu: 5.194 ± 0.6
1.937SerMet: 1.937 ± 0.443
4.05SerAsn: 4.05 ± 1.01
1.321SerPro: 1.321 ± 0.323
2.905SerGln: 2.905 ± 0.54
2.905SerArg: 2.905 ± 0.553
4.93SerSer: 4.93 ± 0.699
5.106SerThr: 5.106 ± 1.226
3.698SerVal: 3.698 ± 0.657
0.792SerTrp: 0.792 ± 0.248
2.201SerTyr: 2.201 ± 0.465
0.0SerXaa: 0.0 ± 0.0
Thr
5.722ThrAla: 5.722 ± 1.592
0.528ThrCys: 0.528 ± 0.182
3.962ThrAsp: 3.962 ± 0.507
4.05ThrGlu: 4.05 ± 0.613
3.257ThrPhe: 3.257 ± 0.457
4.49ThrGly: 4.49 ± 0.724
0.88ThrHis: 0.88 ± 0.291
4.578ThrIle: 4.578 ± 0.792
5.106ThrLys: 5.106 ± 0.821
5.634ThrLeu: 5.634 ± 0.625
0.968ThrMet: 0.968 ± 0.279
3.521ThrAsn: 3.521 ± 0.692
2.641ThrPro: 2.641 ± 0.679
2.817ThrGln: 2.817 ± 0.508
1.321ThrArg: 1.321 ± 0.301
4.754ThrSer: 4.754 ± 0.846
4.754ThrThr: 4.754 ± 0.879
5.458ThrVal: 5.458 ± 0.939
0.44ThrTrp: 0.44 ± 0.17
2.553ThrTyr: 2.553 ± 0.478
0.0ThrXaa: 0.0 ± 0.0
Val
4.666ValAla: 4.666 ± 0.761
0.44ValCys: 0.44 ± 0.211
4.754ValAsp: 4.754 ± 0.866
4.314ValGlu: 4.314 ± 0.678
1.673ValPhe: 1.673 ± 0.42
4.138ValGly: 4.138 ± 0.687
0.88ValHis: 0.88 ± 0.263
4.138ValIle: 4.138 ± 0.558
5.194ValLys: 5.194 ± 0.746
4.93ValLeu: 4.93 ± 0.789
1.056ValMet: 1.056 ± 0.291
3.257ValAsn: 3.257 ± 0.49
2.113ValPro: 2.113 ± 0.47
1.761ValGln: 1.761 ± 0.342
2.553ValArg: 2.553 ± 0.472
6.427ValSer: 6.427 ± 0.761
4.93ValThr: 4.93 ± 0.926
4.842ValVal: 4.842 ± 0.502
1.321ValTrp: 1.321 ± 0.428
2.377ValTyr: 2.377 ± 0.469
0.0ValXaa: 0.0 ± 0.0
Trp
0.352TrpAla: 0.352 ± 0.177
0.352TrpCys: 0.352 ± 0.157
0.44TrpAsp: 0.44 ± 0.164
1.056TrpGlu: 1.056 ± 0.431
0.88TrpPhe: 0.88 ± 0.313
0.704TrpGly: 0.704 ± 0.274
0.088TrpHis: 0.088 ± 0.085
0.352TrpIle: 0.352 ± 0.179
1.056TrpLys: 1.056 ± 0.314
1.321TrpLeu: 1.321 ± 0.31
0.088TrpMet: 0.088 ± 0.086
1.321TrpAsn: 1.321 ± 0.814
0.088TrpPro: 0.088 ± 0.078
0.792TrpGln: 0.792 ± 0.173
0.352TrpArg: 0.352 ± 0.17
1.144TrpSer: 1.144 ± 0.327
1.321TrpThr: 1.321 ± 0.403
0.704TrpVal: 0.704 ± 0.215
0.44TrpTrp: 0.44 ± 0.174
0.88TrpTyr: 0.88 ± 0.333
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.849TyrAla: 1.849 ± 0.361
0.264TyrCys: 0.264 ± 0.151
4.402TyrAsp: 4.402 ± 0.908
2.201TyrGlu: 2.201 ± 0.441
1.849TyrPhe: 1.849 ± 0.311
2.641TyrGly: 2.641 ± 0.687
0.352TyrHis: 0.352 ± 0.19
3.433TyrIle: 3.433 ± 0.631
2.553TyrLys: 2.553 ± 0.56
3.081TyrLeu: 3.081 ± 0.719
1.233TyrMet: 1.233 ± 0.253
2.465TyrAsn: 2.465 ± 0.454
1.233TyrPro: 1.233 ± 0.349
1.761TyrGln: 1.761 ± 0.523
1.673TyrArg: 1.673 ± 0.524
3.257TyrSer: 3.257 ± 0.534
2.817TyrThr: 2.817 ± 0.58
1.937TyrVal: 1.937 ± 0.353
0.616TyrTrp: 0.616 ± 0.227
1.937TyrTyr: 1.937 ± 0.47
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (11360 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski