Acetobacter oeni
Average proteome isoelectric point is 6.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3655 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A511XIX5|A0A511XIX5_9PROT Biotin--[acetyl-CoA-carboxylase] ligase OS=Acetobacter oeni OX=304077 GN=AOE01nite_10850 PE=4 SV=1
MM1 pKa = 7.6 AFFTGYY7 pKa = 8.93 MGLVAGGPASIANEE21 pKa = 4.06 VSAGGYY27 pKa = 9.93 ARR29 pKa = 11.84 LPVSFSSPGDD39 pKa = 3.31 GCLTVAASSSYY50 pKa = 10.39 IYY52 pKa = 11.18 GLATEE57 pKa = 4.54 DD58 pKa = 2.98 WGLITGIAIYY68 pKa = 10.62 SGTTPDD74 pKa = 3.91 EE75 pKa = 4.65 SPVATWAVRR84 pKa = 11.84 PRR86 pKa = 11.84 SLSLGQTYY94 pKa = 7.47 TVPLAALSLLIEE106 pKa = 4.18 PRR108 pKa = 11.84 AFFDD112 pKa = 4.44 DD113 pKa = 4.38 GDD115 pKa = 4.19 VLGVTAGGADD125 pKa = 3.93 IIAGQPLMFTDD136 pKa = 5.31 GVLTPASDD144 pKa = 4.01 GSSSSGSLTLAQLSTLVSEE163 pKa = 5.26 LMQSLPEE170 pKa = 5.05 DD171 pKa = 3.98 DD172 pKa = 5.29 PGDD175 pKa = 4.02 GTSLWVNSGLLAISRR190 pKa = 11.84 SSS192 pKa = 2.93
Molecular weight: 19.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.656
IPC_protein 3.617
Toseland 3.401
ProMoST 3.821
Dawson 3.63
Bjellqvist 3.795
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.325
Solomon 3.605
Lehninger 3.567
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.605
Sillero 3.745
Patrickios 0.769
IPC_peptide 3.605
IPC2_peptide 3.719
IPC2.peptide.svr19 3.718
Protein with the highest isoelectric point:
>tr|A0A511XQF3|A0A511XQF3_9PROT DNA-binding protein OS=Acetobacter oeni OX=304077 GN=AOE01nite_33890 PE=4 SV=1
MM1 pKa = 7.51 IKK3 pKa = 10.48 KK4 pKa = 10.19 LDD6 pKa = 3.52 ARR8 pKa = 11.84 GDD10 pKa = 3.63 RR11 pKa = 11.84 EE12 pKa = 4.03 IAPNEE17 pKa = 3.85 ANVVRR22 pKa = 11.84 RR23 pKa = 11.84 IFRR26 pKa = 11.84 DD27 pKa = 4.36 FAAGIGPRR35 pKa = 11.84 AIARR39 pKa = 11.84 TLNDD43 pKa = 3.35 EE44 pKa = 5.05 SIPGPNGKK52 pKa = 9.12 LWIDD56 pKa = 3.26 TTIRR60 pKa = 11.84 GHH62 pKa = 4.69 VQRR65 pKa = 11.84 GTGIINNEE73 pKa = 4.17 LYY75 pKa = 10.7 VGRR78 pKa = 11.84 LVWNRR83 pKa = 11.84 LRR85 pKa = 11.84 YY86 pKa = 9.73 IKK88 pKa = 10.62 DD89 pKa = 3.08 PSTGKK94 pKa = 10.14 RR95 pKa = 11.84 VSRR98 pKa = 11.84 LNPEE102 pKa = 4.12 SEE104 pKa = 4.23 WIITEE109 pKa = 4.12 VPEE112 pKa = 4.97 LRR114 pKa = 11.84 IVDD117 pKa = 3.8 DD118 pKa = 4.87 ALWQAVRR125 pKa = 11.84 EE126 pKa = 4.16 RR127 pKa = 11.84 QAKK130 pKa = 9.52 IAAQYY135 pKa = 10.51 VNVISAVRR143 pKa = 11.84 ASAANRR149 pKa = 11.84 MNGMRR154 pKa = 11.84 RR155 pKa = 11.84 PKK157 pKa = 10.45 SLFSGMVFCGVCGGTYY173 pKa = 9.16 SLRR176 pKa = 11.84 EE177 pKa = 3.97 KK178 pKa = 10.89 DD179 pKa = 5.21 RR180 pKa = 11.84 YY181 pKa = 10.11 ACSNRR186 pKa = 11.84 IANRR190 pKa = 11.84 SCHH193 pKa = 5.57 NKK195 pKa = 8.6 ATILRR200 pKa = 11.84 PPTLRR205 pKa = 11.84 SACWPASSTSS215 pKa = 3.16
Molecular weight: 24.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.575
IPC_protein 10.438
Toseland 10.628
ProMoST 10.35
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.862
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.57
IPC_peptide 10.862
IPC2_peptide 9.663
IPC2.peptide.svr19 8.613
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3655
0
3655
1131415
40
3107
309.6
33.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.593 ± 0.052
1.014 ± 0.013
5.606 ± 0.029
5.24 ± 0.038
3.621 ± 0.025
8.553 ± 0.048
2.289 ± 0.018
5.148 ± 0.028
2.876 ± 0.03
10.083 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.467 ± 0.016
2.672 ± 0.03
5.497 ± 0.034
3.133 ± 0.022
7.222 ± 0.047
6.13 ± 0.039
5.976 ± 0.041
7.237 ± 0.035
1.419 ± 0.016
2.225 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here