Xanthomonas phage PPDBI
Average proteome isoelectric point is 6.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6H0X5T3|A0A6H0X5T3_9CAUD Uncharacterized protein OS=Xanthomonas phage PPDBI OX=2723911 GN=PPDBI_00055 PE=4 SV=1
MM1 pKa = 7.53 EE2 pKa = 6.64 PDD4 pKa = 5.39 FEE6 pKa = 6.52 DD7 pKa = 5.32 CDD9 pKa = 4.24 VDD11 pKa = 6.03 DD12 pKa = 6.5 FPDD15 pKa = 4.22 DD16 pKa = 3.75 QPDD19 pKa = 3.3 EE20 pKa = 4.25 PFYY23 pKa = 11.14 YY24 pKa = 10.51 EE25 pKa = 5.09 CDD27 pKa = 3.64 DD28 pKa = 6.0 DD29 pKa = 5.7 EE30 pKa = 7.87 DD31 pKa = 5.29 IDD33 pKa = 4.55 VDD35 pKa = 3.79 TCLGCGRR42 pKa = 11.84 ATGVHH47 pKa = 6.43 PGVLAWDD54 pKa = 3.79 QRR56 pKa = 11.84 RR57 pKa = 11.84 EE58 pKa = 4.06 GCIAPQLCPRR68 pKa = 11.84 CWIDD72 pKa = 5.12 QGHH75 pKa = 7.2 PWPSAPP81 pKa = 4.71
Molecular weight: 9.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.829
IPC2_protein 3.579
IPC_protein 3.579
Toseland 3.35
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.567
Rodwell 3.414
Grimsley 3.261
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.986
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.706
Patrickios 0.248
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.676
Protein with the highest isoelectric point:
>tr|A0A6H0X629|A0A6H0X629_9CAUD Uncharacterized protein OS=Xanthomonas phage PPDBI OX=2723911 GN=PPDBI_00064 PE=4 SV=1
MM1 pKa = 7.08 QFSRR5 pKa = 11.84 DD6 pKa = 3.29 VRR8 pKa = 11.84 HH9 pKa = 5.63 YY10 pKa = 10.54 RR11 pKa = 11.84 STPRR15 pKa = 11.84 SLSSSKK21 pKa = 10.23 FGPYY25 pKa = 10.02 ARR27 pKa = 11.84 LSVEE31 pKa = 3.78 RR32 pKa = 11.84 RR33 pKa = 11.84 KK34 pKa = 10.57 SLASKK39 pKa = 10.83 AMDD42 pKa = 3.11 WAYY45 pKa = 11.27 VIGLGFWIGVMWYY58 pKa = 8.52 LTVGLRR64 pKa = 11.84 AA65 pKa = 3.78
Molecular weight: 7.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.288
IPC2_protein 10.233
IPC_protein 11.155
Toseland 11.038
ProMoST 11.125
Dawson 11.125
Bjellqvist 10.965
Wikipedia 11.462
Rodwell 11.14
Grimsley 11.184
Solomon 11.374
Lehninger 11.316
Nozaki 11.008
DTASelect 10.965
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.067
Patrickios 10.906
IPC_peptide 11.389
IPC2_peptide 10.189
IPC2.peptide.svr19 8.127
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
12361
31
711
179.1
19.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.881 ± 0.378
1.213 ± 0.159
5.663 ± 0.249
5.776 ± 0.463
3.244 ± 0.241
7.872 ± 0.376
1.934 ± 0.218
5.0 ± 0.167
4.773 ± 0.315
7.952 ± 0.258
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.896 ± 0.165
4.15 ± 0.395
5.016 ± 0.238
4.967 ± 0.399
5.639 ± 0.382
5.881 ± 0.336
6.027 ± 0.427
6.464 ± 0.266
1.861 ± 0.15
2.791 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here