Youngiibacter fragilis 232.1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Youngiibacter; Youngiibacter fragilis

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V7I4L7|V7I4L7_9CLOT 50S ribosomal protein L35 OS=Youngiibacter fragilis 232.1 OX=994573 GN=rpmI PE=3 SV=1
MM1 pKa = 7.51IVTGSSIIQPTSLTLLPSAITEE23 pKa = 3.67IHH25 pKa = 6.34PTDD28 pKa = 4.77LYY30 pKa = 10.07PTVLTIDD37 pKa = 3.54SVTPADD43 pKa = 4.63ADD45 pKa = 3.6LTDD48 pKa = 4.44IYY50 pKa = 10.29WISTDD55 pKa = 3.43PSVATVVKK63 pKa = 9.76TGLLTADD70 pKa = 3.58VAAKK74 pKa = 10.47SNGYY78 pKa = 9.68AAIQVFRR85 pKa = 11.84NDD87 pKa = 3.14GLFLGSCPVTVTTDD101 pKa = 3.12EE102 pKa = 4.35TLTDD106 pKa = 3.7PVYY109 pKa = 10.74IQATDD114 pKa = 3.77FNGVPLDD121 pKa = 3.66QFPDD125 pKa = 3.39KK126 pKa = 11.08DD127 pKa = 4.03SIYY130 pKa = 8.8ITCYY134 pKa = 10.89NLDD137 pKa = 3.83THH139 pKa = 7.14PGPYY143 pKa = 9.98AIRR146 pKa = 11.84VSEE149 pKa = 4.22KK150 pKa = 10.71SSDD153 pKa = 4.02PILGMNLIDD162 pKa = 3.97GVKK165 pKa = 10.44DD166 pKa = 3.35LVISEE171 pKa = 4.3GQSTYY176 pKa = 8.01MFRR179 pKa = 11.84LIDD182 pKa = 3.78YY183 pKa = 10.89VDD185 pKa = 5.24FEE187 pKa = 4.58PTTNFSKK194 pKa = 11.31SNFVSMSLSDD204 pKa = 3.87NFPTGDD210 pKa = 4.04YY211 pKa = 11.09EE212 pKa = 6.03DD213 pKa = 4.47GTAQTLTDD221 pKa = 3.75NFKK224 pKa = 9.48VTSPVPTGDD233 pKa = 2.79IDD235 pKa = 3.86VDD237 pKa = 3.54IRR239 pKa = 11.84QLDD242 pKa = 3.76QFDD245 pKa = 3.71KK246 pKa = 11.18ASAYY250 pKa = 9.03LHH252 pKa = 6.28PSIIGMNVILGRR264 pKa = 11.84EE265 pKa = 4.17IKK267 pKa = 10.45DD268 pKa = 3.26QTVLEE273 pKa = 4.46TTYY276 pKa = 11.51EE277 pKa = 4.25DD278 pKa = 4.18YY279 pKa = 11.46LNPAWYY285 pKa = 9.15EE286 pKa = 4.1GAPSTIPKK294 pKa = 8.54YY295 pKa = 9.14TDD297 pKa = 3.0EE298 pKa = 4.94AKK300 pKa = 10.93LIGQVADD307 pKa = 3.21VDD309 pKa = 4.37LDD311 pKa = 3.48GDD313 pKa = 4.8YY314 pKa = 10.7EE315 pKa = 4.56VHH317 pKa = 6.47WNPPKK322 pKa = 10.66EE323 pKa = 3.86PLNIGGYY330 pKa = 9.69VLLII334 pKa = 4.1

Molecular weight: 36.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V7HZJ8|V7HZJ8_9CLOT Mannose-6-phosphate isomerase OS=Youngiibacter fragilis 232.1 OX=994573 GN=T472_0218865 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.19EE3 pKa = 3.82RR4 pKa = 11.84LRR6 pKa = 11.84KK7 pKa = 9.83LMQGRR12 pKa = 11.84YY13 pKa = 9.55GVDD16 pKa = 2.83EE17 pKa = 4.8LSRR20 pKa = 11.84LMVYY24 pKa = 10.41VSFVVMLIGSFAKK37 pKa = 10.26NPYY40 pKa = 9.11INLVGFAMTIYY51 pKa = 9.83AYY53 pKa = 10.93SRR55 pKa = 11.84IFSKK59 pKa = 9.19NHH61 pKa = 6.19RR62 pKa = 11.84LCSSQNLKK70 pKa = 10.21YY71 pKa = 10.65VQVRR75 pKa = 11.84DD76 pKa = 3.22RR77 pKa = 11.84FLRR80 pKa = 11.84KK81 pKa = 9.02IANHH85 pKa = 4.73VQIMKK90 pKa = 10.56LSRR93 pKa = 11.84TYY95 pKa = 10.29RR96 pKa = 11.84VYY98 pKa = 10.78SCPGCRR104 pKa = 11.84QMVRR108 pKa = 11.84IPKK111 pKa = 10.06GKK113 pKa = 10.08GKK115 pKa = 10.75VEE117 pKa = 4.22VKK119 pKa = 10.4CPKK122 pKa = 10.02CGTRR126 pKa = 11.84FSRR129 pKa = 11.84KK130 pKa = 7.0SS131 pKa = 3.12

Molecular weight: 15.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3729

0

3729

1132455

29

7056

303.7

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.561 ± 0.049

0.995 ± 0.016

5.784 ± 0.034

7.315 ± 0.047

4.161 ± 0.031

7.818 ± 0.044

1.626 ± 0.019

7.667 ± 0.037

6.574 ± 0.038

9.296 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.214 ± 0.023

3.993 ± 0.022

3.443 ± 0.022

2.242 ± 0.019

5.023 ± 0.044

6.532 ± 0.034

5.258 ± 0.033

7.112 ± 0.035

0.779 ± 0.011

3.608 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski