Eimeria tenella (Coccidian parasite)
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8595 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U6KV80|U6KV80_EIMTE Uncharacterized protein OS=Eimeria tenella OX=5802 GN=ETH_00020980 PE=4 SV=1
MM1 pKa = 7.08 EE2 pKa = 5.34 AAAATDD8 pKa = 3.57 TAAAAAATGVAAATAAEE25 pKa = 4.49 VTTEE29 pKa = 3.73 AAAEE33 pKa = 4.25 TSCAAVQAAQATFAVSGAAASAADD57 pKa = 3.53 EE58 pKa = 4.31 AAANRR63 pKa = 11.84 IDD65 pKa = 3.85 FVRR68 pKa = 11.84 AADD71 pKa = 3.71 TAAAAAAADD80 pKa = 4.08 ADD82 pKa = 3.92 AQKK85 pKa = 10.96 PIMKK89 pKa = 9.66 EE90 pKa = 4.17 LYY92 pKa = 10.33 ASAAAAADD100 pKa = 3.53 AATAGIAADD109 pKa = 3.94 AAGEE113 pKa = 4.06 AADD116 pKa = 4.21 AAAGAADD123 pKa = 3.99 AAAAAADD130 pKa = 3.84 AAADD134 pKa = 3.48 AAAAADD140 pKa = 3.66 ASTEE144 pKa = 3.97 TSAAATDD151 pKa = 3.98 TSSLGAAAADD161 pKa = 3.68 VAAADD166 pKa = 3.89 LAAAAEE172 pKa = 4.32 VATAEE177 pKa = 4.25 LAEE180 pKa = 4.37 AAGDD184 pKa = 3.97 PADD187 pKa = 4.1 AAADD191 pKa = 3.59 AAAAAAEE198 pKa = 4.32 AACDD202 pKa = 3.59 ACNFCADD209 pKa = 3.84 FCSNSWCLRR218 pKa = 11.84 CLPLRR223 pKa = 11.84 QQTRR227 pKa = 11.84 EE228 pKa = 4.11 RR229 pKa = 11.84 LWGPCCCSCCSCCFCNTAAAAEE251 pKa = 4.0 AAAAAEE257 pKa = 4.38 AGFAAEE263 pKa = 4.89 PNSSSIDD270 pKa = 3.57 SYY272 pKa = 11.25 NAAVAAAAALAADD285 pKa = 4.18 RR286 pKa = 11.84 RR287 pKa = 11.84 RR288 pKa = 11.84 RR289 pKa = 11.84 GG290 pKa = 3.1
Molecular weight: 27.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.643
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.821
DTASelect 4.05
Thurlkill 3.554
EMBOSS 3.656
Sillero 3.834
Patrickios 0.515
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|U6KV52|U6KV52_EIMTE Rna polymerase ii b3 subunit OS=Eimeria tenella OX=5802 GN=ETH_00035430 PE=4 SV=1
RR1 pKa = 6.82 PTALVLPFGTHH12 pKa = 5.75 SSSSSSNSSSSSSSKK27 pKa = 10.39 NCSSTSSRR35 pKa = 11.84 RR36 pKa = 11.84 VKK38 pKa = 10.26 RR39 pKa = 11.84 MRR41 pKa = 11.84 MKK43 pKa = 10.53 RR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 SGKK49 pKa = 9.51 NSSKK53 pKa = 9.69 TSSSSSSKK61 pKa = 9.47 SSRR64 pKa = 11.84 RR65 pKa = 11.84 SRR67 pKa = 11.84 SKK69 pKa = 9.87 RR70 pKa = 11.84 SSSRR74 pKa = 11.84 RR75 pKa = 11.84 SSSS78 pKa = 2.78
Molecular weight: 8.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.472
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.471
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.29
Sillero 12.778
Patrickios 12.193
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.097
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8595
0
8595
4341326
10
9860
505.1
54.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.753 ± 0.089
2.015 ± 0.017
3.612 ± 0.021
6.173 ± 0.03
2.743 ± 0.018
6.457 ± 0.031
2.07 ± 0.011
2.431 ± 0.021
3.794 ± 0.029
10.069 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.387 ± 0.011
2.229 ± 0.013
6.093 ± 0.032
9.034 ± 0.079
6.151 ± 0.025
9.219 ± 0.045
4.341 ± 0.022
4.956 ± 0.025
1.0 ± 0.007
1.472 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here