Marinomonas sp. S3726
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4618 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F4QT64|A0A0F4QT64_9GAMM Ion-translocating oxidoreductase complex subunit E OS=Marinomonas sp. S3726 OX=579484 GN=rnfE PE=3 SV=1
MM1 pKa = 7.63 AIVYY5 pKa = 7.59 GTDD8 pKa = 3.12 TNNTEE13 pKa = 4.2 EE14 pKa = 4.35 IAHH17 pKa = 6.98 KK18 pKa = 10.07 IAKK21 pKa = 8.73 QWTEE25 pKa = 3.81 LGHH28 pKa = 4.64 TTEE31 pKa = 4.97 IFNISDD37 pKa = 3.21 IDD39 pKa = 4.3 ADD41 pKa = 3.82 TLAQYY46 pKa = 10.38 EE47 pKa = 4.17 FLILGIPTWDD57 pKa = 3.3 FGGIQSDD64 pKa = 3.72 WEE66 pKa = 4.78 DD67 pKa = 3.14 IGEE70 pKa = 4.43 DD71 pKa = 4.48 LAALDD76 pKa = 4.98 LKK78 pKa = 11.16 GKK80 pKa = 10.53 LIALYY85 pKa = 10.83 GLGDD89 pKa = 3.4 QFGYY93 pKa = 11.19 GDD95 pKa = 4.1 YY96 pKa = 10.82 FVDD99 pKa = 3.43 AMGWIYY105 pKa = 11.01 EE106 pKa = 4.09 HH107 pKa = 6.95 LKK109 pKa = 11.05 DD110 pKa = 5.1 SGATFIGTWSTEE122 pKa = 4.04 GYY124 pKa = 10.85 DD125 pKa = 3.83 FTASRR130 pKa = 11.84 ACIEE134 pKa = 4.61 NNDD137 pKa = 3.69 QFIGLAIDD145 pKa = 3.91 EE146 pKa = 4.65 DD147 pKa = 4.13 QQFEE151 pKa = 4.46 LTDD154 pKa = 3.39 EE155 pKa = 4.92 RR156 pKa = 11.84 VEE158 pKa = 3.79 QWVIQLYY165 pKa = 10.71 AEE167 pKa = 4.15 MMAEE171 pKa = 4.17 AEE173 pKa = 4.21
Molecular weight: 19.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.719
IPC_protein 3.706
Toseland 3.503
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.884
Wikipedia 3.617
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.63
Nozaki 3.808
DTASelect 4.012
Thurlkill 3.554
EMBOSS 3.617
Sillero 3.821
Patrickios 1.036
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A0F4R897|A0A0F4R897_9GAMM SnoaL-like domain-containing protein OS=Marinomonas sp. S3726 OX=579484 GN=TW85_01560 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.24 GGRR28 pKa = 11.84 AVITRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 SLSAA44 pKa = 3.93
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4618
0
4618
1449987
37
4016
314.0
34.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.56 ± 0.036
1.032 ± 0.011
5.578 ± 0.028
6.401 ± 0.032
4.291 ± 0.025
6.541 ± 0.034
2.167 ± 0.02
6.629 ± 0.027
5.734 ± 0.032
10.816 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.577 ± 0.019
4.383 ± 0.027
3.699 ± 0.022
4.511 ± 0.03
4.097 ± 0.027
7.074 ± 0.031
5.079 ± 0.026
6.6 ± 0.032
1.194 ± 0.013
3.037 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here