Marinomonas sp. S3726 
Average proteome isoelectric point is 6.02 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 4618 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|A0A0F4QT64|A0A0F4QT64_9GAMM Ion-translocating oxidoreductase complex subunit E OS=Marinomonas sp. S3726 OX=579484 GN=rnfE PE=3 SV=1 
MM1 pKa = 7.63  AIVYY5 pKa = 7.59  GTDD8 pKa = 3.12  TNNTEE13 pKa = 4.2  EE14 pKa = 4.35  IAHH17 pKa = 6.98  KK18 pKa = 10.07  IAKK21 pKa = 8.73  QWTEE25 pKa = 3.81  LGHH28 pKa = 4.64  TTEE31 pKa = 4.97  IFNISDD37 pKa = 3.21  IDD39 pKa = 4.3  ADD41 pKa = 3.82  TLAQYY46 pKa = 10.38  EE47 pKa = 4.17  FLILGIPTWDD57 pKa = 3.3  FGGIQSDD64 pKa = 3.72  WEE66 pKa = 4.78  DD67 pKa = 3.14  IGEE70 pKa = 4.43  DD71 pKa = 4.48  LAALDD76 pKa = 4.98  LKK78 pKa = 11.16  GKK80 pKa = 10.53  LIALYY85 pKa = 10.83  GLGDD89 pKa = 3.4  QFGYY93 pKa = 11.19  GDD95 pKa = 4.1  YY96 pKa = 10.82  FVDD99 pKa = 3.43  AMGWIYY105 pKa = 11.01  EE106 pKa = 4.09  HH107 pKa = 6.95  LKK109 pKa = 11.05  DD110 pKa = 5.1  SGATFIGTWSTEE122 pKa = 4.04  GYY124 pKa = 10.85  DD125 pKa = 3.83  FTASRR130 pKa = 11.84  ACIEE134 pKa = 4.61  NNDD137 pKa = 3.69  QFIGLAIDD145 pKa = 3.91  EE146 pKa = 4.65  DD147 pKa = 4.13  QQFEE151 pKa = 4.46  LTDD154 pKa = 3.39  EE155 pKa = 4.92  RR156 pKa = 11.84  VEE158 pKa = 3.79  QWVIQLYY165 pKa = 10.71  AEE167 pKa = 4.15  MMAEE171 pKa = 4.17  AEE173 pKa = 4.21   
 Molecular weight: 19.55 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.71 
IPC2_protein 3.719 
IPC_protein 3.706 
Toseland    3.503 
ProMoST     3.846 
Dawson      3.694 
Bjellqvist  3.884 
Wikipedia   3.617 
Rodwell     3.541 
Grimsley    3.414 
Solomon     3.681 
Lehninger   3.63 
Nozaki      3.808 
DTASelect   4.012 
Thurlkill   3.554 
EMBOSS      3.617 
Sillero     3.821 
Patrickios  1.036 
IPC_peptide 3.681 
IPC2_peptide  3.808 
IPC2.peptide.svr19  3.774 
 Protein with the highest isoelectric point: 
>tr|A0A0F4R897|A0A0F4R897_9GAMM SnoaL-like domain-containing protein OS=Marinomonas sp. S3726 OX=579484 GN=TW85_01560 PE=4 SV=1 
MM1 pKa = 7.45  KK2 pKa = 9.51  RR3 pKa = 11.84  TFQPSVLKK11 pKa = 10.46  RR12 pKa = 11.84  KK13 pKa = 9.37  RR14 pKa = 11.84  NHH16 pKa = 5.37  GFRR19 pKa = 11.84  ARR21 pKa = 11.84  MATKK25 pKa = 10.24  GGRR28 pKa = 11.84  AVITRR33 pKa = 11.84  RR34 pKa = 11.84  RR35 pKa = 11.84  ARR37 pKa = 11.84  GRR39 pKa = 11.84  KK40 pKa = 9.03  SLSAA44 pKa = 3.93   
 Molecular weight: 5.1 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.523 
IPC2_protein 11.242 
IPC_protein 12.837 
Toseland    13.013 
ProMoST     13.495 
Dawson      13.013 
Bjellqvist  12.998 
Wikipedia   13.481 
Rodwell     12.676 
Grimsley    13.042 
Solomon     13.495 
Lehninger   13.408 
Nozaki      13.013 
DTASelect   12.998 
Thurlkill   13.013 
EMBOSS      13.51 
Sillero     13.013 
Patrickios  12.398 
IPC_peptide 13.51 
IPC2_peptide  12.486 
IPC2.peptide.svr19  9.177 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        4618 
 
        
        0
 
        
        4618 
         
        1449987
 
        37
 
        4016
 
        314.0
 
        34.91
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        8.56 ± 0.036
1.032 ± 0.011
 
        5.578 ± 0.028
6.401 ± 0.032
 
        4.291 ± 0.025
6.541 ± 0.034
 
        2.167 ± 0.02
6.629 ± 0.027
       
        5.734 ± 0.032
10.816 ± 0.039
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        2.577 ± 0.019
4.383 ± 0.027
 
        3.699 ± 0.022
4.511 ± 0.03
 
        4.097 ± 0.027
7.074 ± 0.031
 
        5.079 ± 0.026
6.6 ± 0.032
       
        1.194 ± 0.013
3.037 ± 0.019
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here