Streptococcus phage IPP50

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SDG0|A0A1S5SDG0_9CAUD HTH cro/C1-type domain-containing protein OS=Streptococcus phage IPP50 OX=1916188 GN=IPP50_00008 PE=4 SV=1
MM1 pKa = 7.36TKK3 pKa = 10.42SDD5 pKa = 3.89LGPYY9 pKa = 9.9LDD11 pKa = 4.98AVTNEE16 pKa = 4.42DD17 pKa = 3.65GTLLICKK24 pKa = 8.59TEE26 pKa = 3.77QGAYY30 pKa = 9.48IGDD33 pKa = 4.28FNPSCDD39 pKa = 3.67EE40 pKa = 3.84EE41 pKa = 5.78DD42 pKa = 3.78FVLTYY47 pKa = 11.02EE48 pKa = 4.44DD49 pKa = 3.86VSVSLSYY56 pKa = 11.13AQVLSATLLKK66 pKa = 10.84VV67 pKa = 3.17

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SDT3|A0A1S5SDT3_9CAUD CI-like repressor OS=Streptococcus phage IPP50 OX=1916188 GN=IPP50_00003 PE=4 SV=1
MM1 pKa = 7.5PRR3 pKa = 11.84SLYY6 pKa = 9.91WKK8 pKa = 10.26DD9 pKa = 3.32EE10 pKa = 4.0YY11 pKa = 10.77TEE13 pKa = 4.1YY14 pKa = 10.0MHH16 pKa = 6.89EE17 pKa = 4.1ICPGRR22 pKa = 11.84LTPEE26 pKa = 3.75VTRR29 pKa = 11.84LLNEE33 pKa = 4.12KK34 pKa = 10.51FGTNYY39 pKa = 10.38NKK41 pKa = 10.51SQIGGVRR48 pKa = 11.84KK49 pKa = 9.89RR50 pKa = 11.84LGLAVGKK57 pKa = 10.01VYY59 pKa = 10.4QGRR62 pKa = 11.84LLTKK66 pKa = 9.04EE67 pKa = 3.56QHH69 pKa = 6.89DD70 pKa = 4.5YY71 pKa = 11.1LVSIQKK77 pKa = 10.36NKK79 pKa = 9.74ISRR82 pKa = 11.84NVANEE87 pKa = 3.76MNLKK91 pKa = 10.42FGLSLTEE98 pKa = 4.06KK99 pKa = 10.22QIKK102 pKa = 9.48SYY104 pKa = 10.67RR105 pKa = 11.84RR106 pKa = 11.84NNNLHH111 pKa = 6.24SGLTGRR117 pKa = 11.84FEE119 pKa = 5.1KK120 pKa = 11.07GQTPHH125 pKa = 6.58NKK127 pKa = 8.94GKK129 pKa = 10.51KK130 pKa = 8.0YY131 pKa = 10.86PNMPKK136 pKa = 10.38NSGQFKK142 pKa = 10.28KK143 pKa = 10.93GNRR146 pKa = 11.84PPNYY150 pKa = 9.98VPVGTINYY158 pKa = 7.06TTDD161 pKa = 3.55GYY163 pKa = 9.73PKK165 pKa = 10.44EE166 pKa = 4.95KK167 pKa = 9.75IGEE170 pKa = 4.09PNQWVLKK177 pKa = 9.51HH178 pKa = 5.61RR179 pKa = 11.84KK180 pKa = 8.01VWEE183 pKa = 4.09EE184 pKa = 3.39HH185 pKa = 6.51HH186 pKa = 6.78GPIPKK191 pKa = 9.74GHH193 pKa = 6.74SIVFLDD199 pKa = 4.36GDD201 pKa = 3.75KK202 pKa = 11.06TNYY205 pKa = 10.28DD206 pKa = 3.57ISNLACLSKK215 pKa = 11.52NEE217 pKa = 4.07IARR220 pKa = 11.84MNQNHH225 pKa = 7.16LFTSNADD232 pKa = 3.59LTKK235 pKa = 10.82SGIGLTKK242 pKa = 9.48LTNKK246 pKa = 9.28IRR248 pKa = 11.84EE249 pKa = 4.16VEE251 pKa = 4.19KK252 pKa = 11.28NGG254 pKa = 3.57

Molecular weight: 29.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12749

38

1871

205.6

23.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.801 ± 0.619

0.541 ± 0.091

6.212 ± 0.298

8.032 ± 0.451

3.992 ± 0.271

6.071 ± 0.616

1.459 ± 0.183

6.369 ± 0.275

9.162 ± 0.42

8.307 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.534 ± 0.209

5.412 ± 0.239

2.737 ± 0.253

3.992 ± 0.201

4.455 ± 0.308

6.0 ± 0.325

6.204 ± 0.299

6.361 ± 0.264

1.381 ± 0.14

3.977 ± 0.383
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski