Capybara microvirus Cap3_SP_188
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVR6|A0A4V1FVR6_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_188 OX=2585421 PE=3 SV=1
MM1 pKa = 7.42 FLYY4 pKa = 10.65 SIYY7 pKa = 10.91 DD8 pKa = 3.47 KK9 pKa = 11.14 VASEE13 pKa = 4.05 FSYY16 pKa = 10.73 PIVLKK21 pKa = 10.21 NDD23 pKa = 3.21 CVAIRR28 pKa = 11.84 WFEE31 pKa = 3.92 NSVSQLPAQNYY42 pKa = 8.78 FDD44 pKa = 4.27 YY45 pKa = 11.02 EE46 pKa = 4.53 LYY48 pKa = 10.73 CLSDD52 pKa = 3.17 WSSEE56 pKa = 3.95 DD57 pKa = 4.96 GILSLCKK64 pKa = 10.16 RR65 pKa = 11.84 KK66 pKa = 9.56 IDD68 pKa = 3.75 VPMEE72 pKa = 4.01 EE73 pKa = 4.54 KK74 pKa = 10.78
Molecular weight: 8.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.169
IPC2_protein 4.469
IPC_protein 4.304
Toseland 4.126
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.215
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 0.896
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.321
Protein with the highest isoelectric point:
>tr|A0A4P8W518|A0A4P8W518_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_188 OX=2585421 PE=4 SV=1
MM1 pKa = 8.14 DD2 pKa = 4.03 CTFPLTVKK10 pKa = 10.51 NPRR13 pKa = 11.84 THH15 pKa = 6.41 QYY17 pKa = 10.44 IKK19 pKa = 10.36 VPCGMCIHH27 pKa = 6.43 CRR29 pKa = 11.84 IAKK32 pKa = 9.33 VRR34 pKa = 11.84 DD35 pKa = 2.95 WSLRR39 pKa = 11.84 LIHH42 pKa = 6.87 EE43 pKa = 4.28 NSLWEE48 pKa = 4.4 DD49 pKa = 3.59 SCFVTLTYY57 pKa = 10.91 NDD59 pKa = 3.67 EE60 pKa = 4.25 NLPLTNCGKK69 pKa = 9.79 MSLYY73 pKa = 10.76 KK74 pKa = 10.12 PDD76 pKa = 3.69 FQNFMKK82 pKa = 10.61 RR83 pKa = 11.84 LRR85 pKa = 11.84 FRR87 pKa = 11.84 IPSPIKK93 pKa = 9.9 YY94 pKa = 8.35 YY95 pKa = 11.13 ACGEE99 pKa = 4.16 YY100 pKa = 10.45 GDD102 pKa = 4.24 EE103 pKa = 4.32 GNRR106 pKa = 11.84 PHH108 pKa = 5.85 YY109 pKa = 10.04 HH110 pKa = 6.84 AIIFGLSCKK119 pKa = 10.27 NIRR122 pKa = 11.84 DD123 pKa = 5.22 FMICWDD129 pKa = 3.71 KK130 pKa = 11.66 GFVTVKK136 pKa = 9.9 PVNAQRR142 pKa = 11.84 CRR144 pKa = 11.84 YY145 pKa = 7.85 VCGYY149 pKa = 8.41 IQKK152 pKa = 10.56 KK153 pKa = 8.35 IRR155 pKa = 11.84 KK156 pKa = 8.86 DD157 pKa = 3.3 PKK159 pKa = 9.77 EE160 pKa = 3.75 YY161 pKa = 9.92 QAAYY165 pKa = 10.34 GCLQPPFQLMSRR177 pKa = 11.84 GLGLGYY183 pKa = 10.16 LEE185 pKa = 4.92 KK186 pKa = 10.88 NRR188 pKa = 11.84 EE189 pKa = 4.29 EE190 pKa = 4.12 YY191 pKa = 10.14 WLTTRR196 pKa = 11.84 NTLNGVPLSIPRR208 pKa = 11.84 YY209 pKa = 7.6 YY210 pKa = 10.78 LKK212 pKa = 10.52 KK213 pKa = 10.52 DD214 pKa = 3.06 WFLHH218 pKa = 5.11 TKK220 pKa = 10.32 VGLKK224 pKa = 10.04 SDD226 pKa = 4.03 SKK228 pKa = 10.65 PATEE232 pKa = 4.09 VEE234 pKa = 4.77 SYY236 pKa = 11.39 DD237 pKa = 4.59 SAIQRR242 pKa = 11.84 DD243 pKa = 3.37 KK244 pKa = 11.45 DD245 pKa = 3.62 LRR247 pKa = 11.84 AKK249 pKa = 10.01 TNLKK253 pKa = 9.74 KK254 pKa = 10.61 RR255 pKa = 11.84 KK256 pKa = 9.13 LL257 pKa = 3.61
Molecular weight: 30.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.84
IPC2_protein 8.799
IPC_protein 8.682
Toseland 9.238
ProMoST 9.165
Dawson 9.589
Bjellqvist 9.458
Wikipedia 9.765
Rodwell 9.794
Grimsley 9.648
Solomon 9.633
Lehninger 9.589
Nozaki 9.589
DTASelect 9.355
Thurlkill 9.458
EMBOSS 9.721
Sillero 9.619
Patrickios 4.482
IPC_peptide 9.619
IPC2_peptide 8.361
IPC2.peptide.svr19 7.817
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1339
74
561
267.8
30.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.796 ± 1.067
1.718 ± 0.81
5.452 ± 0.345
5.527 ± 1.333
4.929 ± 0.65
6.423 ± 1.033
1.792 ± 0.356
5.676 ± 0.347
5.825 ± 1.204
8.215 ± 0.734
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.44
5.9 ± 0.87
4.63 ± 1.202
4.556 ± 0.872
4.182 ± 0.877
8.215 ± 1.175
5.676 ± 1.156
5.975 ± 0.831
1.419 ± 0.298
4.854 ± 0.521
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here