Amino acid dipepetide frequency for Gordonia phage Moosehead

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.92AlaAla: 15.92 ± 1.62
0.727AlaCys: 0.727 ± 0.275
8.869AlaAsp: 8.869 ± 0.815
7.415AlaGlu: 7.415 ± 0.772
3.126AlaPhe: 3.126 ± 0.514
9.523AlaGly: 9.523 ± 0.855
1.89AlaHis: 1.89 ± 0.507
5.452AlaIle: 5.452 ± 0.681
3.998AlaLys: 3.998 ± 0.538
8.796AlaLeu: 8.796 ± 0.731
3.926AlaMet: 3.926 ± 0.588
4.071AlaAsn: 4.071 ± 0.6
5.598AlaPro: 5.598 ± 0.866
4.58AlaGln: 4.58 ± 0.451
7.342AlaArg: 7.342 ± 0.833
5.67AlaSer: 5.67 ± 0.793
7.706AlaThr: 7.706 ± 1.324
7.924AlaVal: 7.924 ± 1.008
2.108AlaTrp: 2.108 ± 0.382
1.89AlaTyr: 1.89 ± 0.348
0.0AlaXaa: 0.0 ± 0.0
Cys
1.236CysAla: 1.236 ± 0.272
0.073CysCys: 0.073 ± 0.067
0.436CysAsp: 0.436 ± 0.193
0.727CysGlu: 0.727 ± 0.257
0.073CysPhe: 0.073 ± 0.085
1.745CysGly: 1.745 ± 0.405
0.436CysHis: 0.436 ± 0.186
0.073CysIle: 0.073 ± 0.082
0.218CysLys: 0.218 ± 0.127
0.8CysLeu: 0.8 ± 0.209
0.073CysMet: 0.073 ± 0.069
0.291CysAsn: 0.291 ± 0.153
0.872CysPro: 0.872 ± 0.237
0.218CysGln: 0.218 ± 0.163
0.727CysArg: 0.727 ± 0.223
0.363CysSer: 0.363 ± 0.186
0.363CysThr: 0.363 ± 0.179
0.436CysVal: 0.436 ± 0.19
0.363CysTrp: 0.363 ± 0.172
0.436CysTyr: 0.436 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
6.979AspAla: 6.979 ± 0.622
0.363AspCys: 0.363 ± 0.188
4.434AspAsp: 4.434 ± 0.678
4.434AspGlu: 4.434 ± 0.55
2.254AspPhe: 2.254 ± 0.32
6.543AspGly: 6.543 ± 0.6
2.181AspHis: 2.181 ± 0.402
3.199AspIle: 3.199 ± 0.595
1.817AspLys: 1.817 ± 0.353
6.543AspLeu: 6.543 ± 0.824
1.018AspMet: 1.018 ± 0.233
2.254AspAsn: 2.254 ± 0.465
4.362AspPro: 4.362 ± 0.649
2.108AspGln: 2.108 ± 0.442
5.743AspArg: 5.743 ± 0.574
3.489AspSer: 3.489 ± 0.498
3.707AspThr: 3.707 ± 0.459
4.725AspVal: 4.725 ± 0.457
1.745AspTrp: 1.745 ± 0.375
1.745AspTyr: 1.745 ± 0.308
0.0AspXaa: 0.0 ± 0.0
Glu
6.906GluAla: 6.906 ± 0.561
0.872GluCys: 0.872 ± 0.264
3.489GluAsp: 3.489 ± 0.509
3.053GluGlu: 3.053 ± 0.416
1.817GluPhe: 1.817 ± 0.363
3.707GluGly: 3.707 ± 0.566
2.035GluHis: 2.035 ± 0.501
2.835GluIle: 2.835 ± 0.447
2.399GluLys: 2.399 ± 0.432
4.507GluLeu: 4.507 ± 0.742
1.527GluMet: 1.527 ± 0.299
1.745GluAsn: 1.745 ± 0.305
3.417GluPro: 3.417 ± 0.553
2.908GluGln: 2.908 ± 0.462
5.089GluArg: 5.089 ± 0.622
3.78GluSer: 3.78 ± 0.562
2.762GluThr: 2.762 ± 0.497
3.635GluVal: 3.635 ± 0.523
1.163GluTrp: 1.163 ± 0.346
1.672GluTyr: 1.672 ± 0.314
0.0GluXaa: 0.0 ± 0.0
Phe
3.199PheAla: 3.199 ± 0.518
0.363PheCys: 0.363 ± 0.175
2.544PheAsp: 2.544 ± 0.505
1.163PheGlu: 1.163 ± 0.393
0.872PhePhe: 0.872 ± 0.186
2.908PheGly: 2.908 ± 0.439
0.145PheHis: 0.145 ± 0.104
1.018PheIle: 1.018 ± 0.283
1.09PheLys: 1.09 ± 0.282
1.163PheLeu: 1.163 ± 0.264
0.363PheMet: 0.363 ± 0.153
1.09PheAsn: 1.09 ± 0.267
1.454PhePro: 1.454 ± 0.286
0.872PheGln: 0.872 ± 0.216
1.599PheArg: 1.599 ± 0.348
0.945PheSer: 0.945 ± 0.221
2.617PheThr: 2.617 ± 0.477
1.745PheVal: 1.745 ± 0.343
0.945PheTrp: 0.945 ± 0.328
0.509PheTyr: 0.509 ± 0.158
0.0PheXaa: 0.0 ± 0.0
Gly
8.287GlyAla: 8.287 ± 0.98
0.727GlyCys: 0.727 ± 0.253
6.325GlyAsp: 6.325 ± 0.636
5.816GlyGlu: 5.816 ± 0.645
1.599GlyPhe: 1.599 ± 0.412
8.796GlyGly: 8.796 ± 0.935
2.326GlyHis: 2.326 ± 0.447
3.635GlyIle: 3.635 ± 0.56
3.417GlyLys: 3.417 ± 0.506
6.688GlyLeu: 6.688 ± 0.992
2.035GlyMet: 2.035 ± 0.353
2.835GlyAsn: 2.835 ± 0.48
4.653GlyPro: 4.653 ± 0.502
2.617GlyGln: 2.617 ± 0.415
6.252GlyArg: 6.252 ± 0.719
5.089GlySer: 5.089 ± 0.963
5.67GlyThr: 5.67 ± 0.769
6.906GlyVal: 6.906 ± 0.796
1.745GlyTrp: 1.745 ± 0.365
2.69GlyTyr: 2.69 ± 0.419
0.0GlyXaa: 0.0 ± 0.0
His
2.326HisAla: 2.326 ± 0.423
0.073HisCys: 0.073 ± 0.07
1.963HisAsp: 1.963 ± 0.396
0.945HisGlu: 0.945 ± 0.313
0.218HisPhe: 0.218 ± 0.134
1.963HisGly: 1.963 ± 0.468
0.8HisHis: 0.8 ± 0.251
1.09HisIle: 1.09 ± 0.291
0.436HisLys: 0.436 ± 0.161
1.817HisLeu: 1.817 ± 0.367
0.436HisMet: 0.436 ± 0.219
0.582HisAsn: 0.582 ± 0.205
1.672HisPro: 1.672 ± 0.422
0.8HisGln: 0.8 ± 0.25
1.599HisArg: 1.599 ± 0.395
1.163HisSer: 1.163 ± 0.371
1.454HisThr: 1.454 ± 0.315
1.309HisVal: 1.309 ± 0.361
0.8HisTrp: 0.8 ± 0.265
0.654HisTyr: 0.654 ± 0.247
0.0HisXaa: 0.0 ± 0.0
Ile
6.47IleAla: 6.47 ± 0.785
0.363IleCys: 0.363 ± 0.167
4.434IleAsp: 4.434 ± 0.53
3.271IleGlu: 3.271 ± 0.499
1.163IlePhe: 1.163 ± 0.351
3.926IleGly: 3.926 ± 0.525
0.872IleHis: 0.872 ± 0.224
1.817IleIle: 1.817 ± 0.341
1.527IleLys: 1.527 ± 0.334
3.053IleLeu: 3.053 ± 0.538
0.436IleMet: 0.436 ± 0.208
1.599IleAsn: 1.599 ± 0.348
2.835IlePro: 2.835 ± 0.482
2.035IleGln: 2.035 ± 0.33
3.562IleArg: 3.562 ± 0.385
2.472IleSer: 2.472 ± 0.412
2.835IleThr: 2.835 ± 0.44
3.417IleVal: 3.417 ± 0.443
1.018IleTrp: 1.018 ± 0.321
0.945IleTyr: 0.945 ± 0.305
0.0IleXaa: 0.0 ± 0.0
Lys
4.871LysAla: 4.871 ± 0.743
0.436LysCys: 0.436 ± 0.175
2.035LysAsp: 2.035 ± 0.314
1.672LysGlu: 1.672 ± 0.418
0.509LysPhe: 0.509 ± 0.2
2.472LysGly: 2.472 ± 0.448
0.727LysHis: 0.727 ± 0.294
1.09LysIle: 1.09 ± 0.27
0.945LysLys: 0.945 ± 0.282
1.745LysLeu: 1.745 ± 0.388
0.8LysMet: 0.8 ± 0.263
1.018LysAsn: 1.018 ± 0.27
2.762LysPro: 2.762 ± 0.516
1.454LysGln: 1.454 ± 0.345
2.762LysArg: 2.762 ± 0.404
2.399LysSer: 2.399 ± 0.531
2.326LysThr: 2.326 ± 0.395
2.254LysVal: 2.254 ± 0.456
0.727LysTrp: 0.727 ± 0.241
0.945LysTyr: 0.945 ± 0.189
0.0LysXaa: 0.0 ± 0.0
Leu
9.087LeuAla: 9.087 ± 0.884
1.018LeuCys: 1.018 ± 0.243
5.67LeuAsp: 5.67 ± 0.672
4.653LeuGlu: 4.653 ± 0.588
1.672LeuPhe: 1.672 ± 0.477
6.47LeuGly: 6.47 ± 0.802
1.454LeuHis: 1.454 ± 0.298
3.998LeuIle: 3.998 ± 0.464
2.472LeuLys: 2.472 ± 0.584
4.362LeuLeu: 4.362 ± 0.516
1.745LeuMet: 1.745 ± 0.422
1.89LeuAsn: 1.89 ± 0.315
3.635LeuPro: 3.635 ± 0.343
1.599LeuGln: 1.599 ± 0.261
5.016LeuArg: 5.016 ± 0.754
4.289LeuSer: 4.289 ± 0.491
5.089LeuThr: 5.089 ± 0.671
5.234LeuVal: 5.234 ± 0.627
1.018LeuTrp: 1.018 ± 0.228
1.599LeuTyr: 1.599 ± 0.306
0.0LeuXaa: 0.0 ± 0.0
Met
2.472MetAla: 2.472 ± 0.465
0.363MetCys: 0.363 ± 0.203
1.163MetAsp: 1.163 ± 0.257
0.654MetGlu: 0.654 ± 0.185
0.727MetPhe: 0.727 ± 0.175
1.89MetGly: 1.89 ± 0.33
0.436MetHis: 0.436 ± 0.197
1.236MetIle: 1.236 ± 0.295
1.018MetLys: 1.018 ± 0.248
1.672MetLeu: 1.672 ± 0.38
0.218MetMet: 0.218 ± 0.117
0.654MetAsn: 0.654 ± 0.233
1.527MetPro: 1.527 ± 0.313
1.163MetGln: 1.163 ± 0.303
1.599MetArg: 1.599 ± 0.33
1.672MetSer: 1.672 ± 0.279
2.254MetThr: 2.254 ± 0.313
1.309MetVal: 1.309 ± 0.272
0.291MetTrp: 0.291 ± 0.131
0.363MetTyr: 0.363 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.417AsnAla: 3.417 ± 0.451
0.363AsnCys: 0.363 ± 0.164
1.236AsnAsp: 1.236 ± 0.29
1.745AsnGlu: 1.745 ± 0.334
1.236AsnPhe: 1.236 ± 0.469
3.853AsnGly: 3.853 ± 0.557
0.945AsnHis: 0.945 ± 0.31
1.163AsnIle: 1.163 ± 0.334
1.09AsnLys: 1.09 ± 0.306
2.326AsnLeu: 2.326 ± 0.362
0.872AsnMet: 0.872 ± 0.247
0.945AsnAsn: 0.945 ± 0.242
2.617AsnPro: 2.617 ± 0.423
1.09AsnGln: 1.09 ± 0.338
1.599AsnArg: 1.599 ± 0.378
1.309AsnSer: 1.309 ± 0.34
1.527AsnThr: 1.527 ± 0.354
1.963AsnVal: 1.963 ± 0.373
0.582AsnTrp: 0.582 ± 0.226
0.509AsnTyr: 0.509 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
6.106ProAla: 6.106 ± 0.684
0.509ProCys: 0.509 ± 0.194
5.525ProAsp: 5.525 ± 0.615
4.216ProGlu: 4.216 ± 0.553
1.309ProPhe: 1.309 ± 0.256
4.653ProGly: 4.653 ± 0.517
0.945ProHis: 0.945 ± 0.252
2.254ProIle: 2.254 ± 0.471
2.326ProLys: 2.326 ± 0.446
3.853ProLeu: 3.853 ± 0.447
1.309ProMet: 1.309 ± 0.306
1.963ProAsn: 1.963 ± 0.417
3.853ProPro: 3.853 ± 0.611
1.309ProGln: 1.309 ± 0.289
3.562ProArg: 3.562 ± 0.813
3.562ProSer: 3.562 ± 0.518
2.908ProThr: 2.908 ± 0.504
3.998ProVal: 3.998 ± 0.499
1.527ProTrp: 1.527 ± 0.279
1.309ProTyr: 1.309 ± 0.352
0.0ProXaa: 0.0 ± 0.0
Gln
4.362GlnAla: 4.362 ± 0.599
0.218GlnCys: 0.218 ± 0.157
1.09GlnAsp: 1.09 ± 0.286
1.381GlnGlu: 1.381 ± 0.288
1.018GlnPhe: 1.018 ± 0.223
2.254GlnGly: 2.254 ± 0.411
0.654GlnHis: 0.654 ± 0.287
2.399GlnIle: 2.399 ± 0.39
1.309GlnLys: 1.309 ± 0.344
2.762GlnLeu: 2.762 ± 0.45
1.018GlnMet: 1.018 ± 0.284
0.363GlnAsn: 0.363 ± 0.158
1.672GlnPro: 1.672 ± 0.432
1.381GlnGln: 1.381 ± 0.371
4.216GlnArg: 4.216 ± 0.573
1.527GlnSer: 1.527 ± 0.426
2.326GlnThr: 2.326 ± 0.397
2.181GlnVal: 2.181 ± 0.394
0.727GlnTrp: 0.727 ± 0.204
1.09GlnTyr: 1.09 ± 0.291
0.0GlnXaa: 0.0 ± 0.0
Arg
8.723ArgAla: 8.723 ± 0.645
1.09ArgCys: 1.09 ± 0.335
5.452ArgAsp: 5.452 ± 0.588
5.016ArgGlu: 5.016 ± 0.57
2.617ArgPhe: 2.617 ± 0.544
4.943ArgGly: 4.943 ± 0.599
1.745ArgHis: 1.745 ± 0.379
3.998ArgIle: 3.998 ± 0.486
2.908ArgLys: 2.908 ± 0.44
5.598ArgLeu: 5.598 ± 0.674
1.527ArgMet: 1.527 ± 0.326
2.399ArgAsn: 2.399 ± 0.484
3.344ArgPro: 3.344 ± 0.555
2.69ArgGln: 2.69 ± 0.413
6.397ArgArg: 6.397 ± 0.844
4.216ArgSer: 4.216 ± 0.621
4.216ArgThr: 4.216 ± 0.512
4.434ArgVal: 4.434 ± 0.512
1.672ArgTrp: 1.672 ± 0.354
1.599ArgTyr: 1.599 ± 0.374
0.0ArgXaa: 0.0 ± 0.0
Ser
6.106SerAla: 6.106 ± 1.122
0.363SerCys: 0.363 ± 0.199
3.199SerAsp: 3.199 ± 0.423
2.981SerGlu: 2.981 ± 0.35
1.817SerPhe: 1.817 ± 0.407
7.27SerGly: 7.27 ± 0.883
1.163SerHis: 1.163 ± 0.293
2.69SerIle: 2.69 ± 0.408
1.454SerLys: 1.454 ± 0.308
3.78SerLeu: 3.78 ± 0.524
1.527SerMet: 1.527 ± 0.272
1.745SerAsn: 1.745 ± 0.352
2.908SerPro: 2.908 ± 0.492
1.381SerGln: 1.381 ± 0.429
3.344SerArg: 3.344 ± 0.59
3.78SerSer: 3.78 ± 0.661
2.762SerThr: 2.762 ± 0.408
4.725SerVal: 4.725 ± 0.799
1.599SerTrp: 1.599 ± 0.314
1.745SerTyr: 1.745 ± 0.394
0.0SerXaa: 0.0 ± 0.0
Thr
8.287ThrAla: 8.287 ± 1.137
0.582ThrCys: 0.582 ± 0.234
3.998ThrAsp: 3.998 ± 0.557
2.399ThrGlu: 2.399 ± 0.381
1.527ThrPhe: 1.527 ± 0.367
6.47ThrGly: 6.47 ± 0.653
0.945ThrHis: 0.945 ± 0.275
4.725ThrIle: 4.725 ± 0.676
1.381ThrLys: 1.381 ± 0.286
4.943ThrLeu: 4.943 ± 0.489
1.309ThrMet: 1.309 ± 0.305
2.035ThrAsn: 2.035 ± 0.581
4.216ThrPro: 4.216 ± 0.536
1.817ThrGln: 1.817 ± 0.384
3.635ThrArg: 3.635 ± 0.484
3.199ThrSer: 3.199 ± 0.555
4.943ThrThr: 4.943 ± 0.695
4.653ThrVal: 4.653 ± 0.704
1.381ThrTrp: 1.381 ± 0.269
1.09ThrTyr: 1.09 ± 0.235
0.0ThrXaa: 0.0 ± 0.0
Val
7.56ValAla: 7.56 ± 0.955
0.872ValCys: 0.872 ± 0.278
4.507ValAsp: 4.507 ± 0.438
5.598ValGlu: 5.598 ± 0.452
2.035ValPhe: 2.035 ± 0.4
5.67ValGly: 5.67 ± 0.673
1.236ValHis: 1.236 ± 0.265
3.344ValIle: 3.344 ± 0.444
2.544ValLys: 2.544 ± 0.5
3.998ValLeu: 3.998 ± 0.659
1.527ValMet: 1.527 ± 0.343
1.745ValAsn: 1.745 ± 0.338
3.053ValPro: 3.053 ± 0.435
1.963ValGln: 1.963 ± 0.367
6.325ValArg: 6.325 ± 0.667
4.362ValSer: 4.362 ± 0.657
5.379ValThr: 5.379 ± 0.643
6.397ValVal: 6.397 ± 0.935
1.09ValTrp: 1.09 ± 0.319
1.527ValTyr: 1.527 ± 0.379
0.0ValXaa: 0.0 ± 0.0
Trp
2.108TrpAla: 2.108 ± 0.359
0.145TrpCys: 0.145 ± 0.106
1.599TrpAsp: 1.599 ± 0.339
0.945TrpGlu: 0.945 ± 0.216
0.436TrpPhe: 0.436 ± 0.156
1.09TrpGly: 1.09 ± 0.268
0.654TrpHis: 0.654 ± 0.202
1.018TrpIle: 1.018 ± 0.277
0.8TrpLys: 0.8 ± 0.218
2.035TrpLeu: 2.035 ± 0.439
0.363TrpMet: 0.363 ± 0.13
0.654TrpAsn: 0.654 ± 0.245
1.454TrpPro: 1.454 ± 0.326
0.8TrpGln: 0.8 ± 0.214
2.399TrpArg: 2.399 ± 0.52
1.381TrpSer: 1.381 ± 0.332
1.599TrpThr: 1.599 ± 0.335
1.454TrpVal: 1.454 ± 0.322
0.509TrpTrp: 0.509 ± 0.178
0.145TrpTyr: 0.145 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.108TyrAla: 2.108 ± 0.382
0.436TyrCys: 0.436 ± 0.182
1.745TyrAsp: 1.745 ± 0.293
1.236TyrGlu: 1.236 ± 0.267
0.582TyrPhe: 0.582 ± 0.162
1.963TyrGly: 1.963 ± 0.281
0.509TyrHis: 0.509 ± 0.203
1.018TyrIle: 1.018 ± 0.264
0.8TyrLys: 0.8 ± 0.22
1.527TyrLeu: 1.527 ± 0.27
0.582TyrMet: 0.582 ± 0.167
0.509TyrAsn: 0.509 ± 0.183
1.09TyrPro: 1.09 ± 0.317
1.163TyrGln: 1.163 ± 0.298
1.817TyrArg: 1.817 ± 0.291
1.672TyrSer: 1.672 ± 0.365
1.09TyrThr: 1.09 ± 0.305
2.035TyrVal: 2.035 ± 0.325
0.582TyrTrp: 0.582 ± 0.205
0.509TyrTyr: 0.509 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (13757 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski