Erythranthe guttata (Yellow monkey flower) (Mimulus guttatus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Lamiales; Phrymaceae; Erythranthe

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28677 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A022Q254|A0A022Q254_ERYGU Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a022953mg PE=3 SV=1
MM1 pKa = 7.57SIYY4 pKa = 10.83GPFQSPFSLLIIFFLLSLSRR24 pKa = 11.84PEE26 pKa = 4.68QMTLICDD33 pKa = 3.84ASVTPMAEE41 pKa = 4.14TQEE44 pKa = 3.8KK45 pKa = 9.86RR46 pKa = 11.84LRR48 pKa = 11.84EE49 pKa = 3.92EE50 pKa = 4.01SHH52 pKa = 7.36DD53 pKa = 4.18SEE55 pKa = 4.45TPEE58 pKa = 4.4YY59 pKa = 11.18DD60 pKa = 4.02DD61 pKa = 5.75ANSNSKK67 pKa = 9.44RR68 pKa = 11.84QKK70 pKa = 9.29PYY72 pKa = 11.41NNILSLLDD80 pKa = 3.81EE81 pKa = 4.81EE82 pKa = 5.87EE83 pKa = 4.1VDD85 pKa = 3.83QDD87 pKa = 4.35EE88 pKa = 5.56EE89 pKa = 4.4PTQDD93 pKa = 3.21LSAIFTTLEE102 pKa = 3.88QEE104 pKa = 4.12ISSTTTATAASYY116 pKa = 11.08DD117 pKa = 3.4VDD119 pKa = 4.44FPPFEE124 pKa = 4.37AAAEE128 pKa = 4.29EE129 pKa = 4.53EE130 pKa = 4.41DD131 pKa = 5.23HH132 pKa = 6.5SQQTAAACSPSSGDD146 pKa = 4.02YY147 pKa = 10.93ADD149 pKa = 4.83EE150 pKa = 5.28DD151 pKa = 4.56DD152 pKa = 4.32GVKK155 pKa = 10.41SVMRR159 pKa = 11.84HH160 pKa = 5.12LLEE163 pKa = 5.67ASDD166 pKa = 4.99DD167 pKa = 3.83EE168 pKa = 5.3LGIPTTEE175 pKa = 4.0DD176 pKa = 3.08NYY178 pKa = 11.26NSVVEE183 pKa = 4.31INYY186 pKa = 10.03SEE188 pKa = 4.16NFPFGFSDD196 pKa = 3.22HH197 pKa = 7.07GIWEE201 pKa = 4.38MEE203 pKa = 3.98DD204 pKa = 3.21VAANYY209 pKa = 8.41YY210 pKa = 10.54TMLQSEE216 pKa = 5.12LFMM219 pKa = 6.79

Molecular weight: 24.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A022RKR6|A0A022RKR6_ERYGU Uncharacterized protein OS=Erythranthe guttata OX=4155 GN=MIMGU_mgv1a023577mg PE=4 SV=1
SS1 pKa = 6.44SRR3 pKa = 11.84TRR5 pKa = 11.84MRR7 pKa = 11.84IKK9 pKa = 10.41KK10 pKa = 9.38RR11 pKa = 11.84AQKK14 pKa = 10.55HH15 pKa = 4.38SLQKK19 pKa = 10.27IRR21 pKa = 11.84TT22 pKa = 3.88

Molecular weight: 2.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

27703

974

28677

10799916

8

5112

376.6

42.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.75 ± 0.013

1.849 ± 0.007

5.355 ± 0.01

6.425 ± 0.014

4.346 ± 0.01

6.56 ± 0.011

2.313 ± 0.006

5.568 ± 0.011

6.104 ± 0.013

9.554 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.007

4.694 ± 0.009

4.847 ± 0.015

3.375 ± 0.01

5.319 ± 0.011

8.784 ± 0.015

4.939 ± 0.008

6.653 ± 0.009

1.279 ± 0.005

2.931 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski