Pan troglodytes verus polyomavirus 8
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5J6XTZ8|A0A5J6XTZ8_9POLY 139 t antigen OS=Pan troglodytes verus polyomavirus 8 OX=1762023 GN=139T PE=4 SV=1
MM1 pKa = 7.91 DD2 pKa = 4.22 KK3 pKa = 10.85 VLNRR7 pKa = 11.84 EE8 pKa = 3.8 EE9 pKa = 4.74 SMEE12 pKa = 4.42 LMDD15 pKa = 5.93 LLGLEE20 pKa = 4.16 RR21 pKa = 11.84 SSWGNLPAMRR31 pKa = 11.84 KK32 pKa = 9.65 AYY34 pKa = 10.05 LKK36 pKa = 10.11 KK37 pKa = 10.51 CKK39 pKa = 9.6 EE40 pKa = 3.81 FHH42 pKa = 6.71 PDD44 pKa = 3.14 KK45 pKa = 11.47 GGDD48 pKa = 3.5 EE49 pKa = 5.03 DD50 pKa = 4.7 KK51 pKa = 10.49 MKK53 pKa = 10.95 RR54 pKa = 11.84 MNTLYY59 pKa = 10.81 KK60 pKa = 10.68 KK61 pKa = 9.75 MEE63 pKa = 4.01 QDD65 pKa = 3.2 VKK67 pKa = 10.58 IAHH70 pKa = 5.94 QPDD73 pKa = 4.13 FGAWNSSEE81 pKa = 3.55 IPTYY85 pKa = 9.72 GTEE88 pKa = 4.06 EE89 pKa = 4.09 WEE91 pKa = 4.41 SWWNSFNEE99 pKa = 4.07 KK100 pKa = 9.17 WDD102 pKa = 3.7 EE103 pKa = 3.81 EE104 pKa = 4.74 LFCHH108 pKa = 6.7 EE109 pKa = 5.9 DD110 pKa = 3.08 MFASDD115 pKa = 4.42 EE116 pKa = 4.12 EE117 pKa = 4.31 ATADD121 pKa = 4.05 SQHH124 pKa = 5.69 STPPKK129 pKa = 9.25 KK130 pKa = 9.7 KK131 pKa = 8.81 RR132 pKa = 11.84 KK133 pKa = 9.43 RR134 pKa = 11.84 RR135 pKa = 11.84 GFRR138 pKa = 11.84 NN139 pKa = 3.22
Molecular weight: 16.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.522
IPC2_protein 5.525
IPC_protein 5.474
Toseland 5.652
ProMoST 5.69
Dawson 5.575
Bjellqvist 5.639
Wikipedia 5.499
Rodwell 5.525
Grimsley 5.69
Solomon 5.575
Lehninger 5.55
Nozaki 5.753
DTASelect 5.919
Thurlkill 5.766
EMBOSS 5.715
Sillero 5.855
Patrickios 4.546
IPC_peptide 5.588
IPC2_peptide 5.842
IPC2.peptide.svr19 5.937
Protein with the highest isoelectric point:
>tr|A0A0S2VEK6|A0A0S2VEK6_9POLY Minor capsid protein OS=Pan troglodytes verus polyomavirus 8 OX=1762023 GN=VP3 PE=3 SV=1
MM1 pKa = 7.53 VLRR4 pKa = 11.84 QLSRR8 pKa = 11.84 QASVKK13 pKa = 10.28 VGKK16 pKa = 7.95 TWTGTKK22 pKa = 9.71 RR23 pKa = 11.84 RR24 pKa = 11.84 AQRR27 pKa = 11.84 IFIFILEE34 pKa = 4.21 ILLEE38 pKa = 4.16 FCRR41 pKa = 11.84 GEE43 pKa = 4.01 DD44 pKa = 4.57 SVDD47 pKa = 3.15 GKK49 pKa = 9.62 QKK51 pKa = 10.36 KK52 pKa = 9.41 RR53 pKa = 11.84 EE54 pKa = 3.99 STLTDD59 pKa = 3.02 KK60 pKa = 10.82 TGYY63 pKa = 10.17
Molecular weight: 7.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.185
IPC2_protein 9.399
IPC_protein 9.516
Toseland 10.54
ProMoST 10.058
Dawson 10.613
Bjellqvist 10.204
Wikipedia 10.73
Rodwell 11.111
Grimsley 10.643
Solomon 10.672
Lehninger 10.657
Nozaki 10.511
DTASelect 10.204
Thurlkill 10.511
EMBOSS 10.921
Sillero 10.526
Patrickios 10.906
IPC_peptide 10.687
IPC2_peptide 8.785
IPC2.peptide.svr19 8.505
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2018
63
691
288.3
32.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.095 ± 1.138
1.982 ± 0.665
5.699 ± 0.546
7.235 ± 0.579
4.46 ± 0.526
5.897 ± 0.636
2.478 ± 0.349
4.708 ± 0.615
7.334 ± 1.211
8.622 ± 0.642
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.825 ± 0.523
4.212 ± 0.379
5.104 ± 0.579
4.163 ± 0.437
5.401 ± 0.682
7.235 ± 0.662
5.798 ± 0.721
5.401 ± 0.679
1.833 ± 0.397
3.518 ± 0.224
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here