Peribacillus deserti
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4462 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N5M8D9|A0A2N5M8D9_9BACI D-alanine aminotransferase OS=Peribacillus deserti OX=673318 GN=dat PE=3 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.44 SFVVSFHH9 pKa = 7.01 QEE11 pKa = 4.01 DD12 pKa = 4.02 NVDD15 pKa = 3.08 TMQIQKK21 pKa = 10.54 LDD23 pKa = 3.59 DD24 pKa = 3.85 TEE26 pKa = 4.79 FDD28 pKa = 3.39 QATEE32 pKa = 3.8 GGARR36 pKa = 11.84 HH37 pKa = 6.68 LFDD40 pKa = 6.16 LDD42 pKa = 3.75 TNIGYY47 pKa = 9.63 FIFMDD52 pKa = 4.52 AEE54 pKa = 5.18 DD55 pKa = 3.82 IDD57 pKa = 5.6 GDD59 pKa = 4.03 VSHH62 pKa = 7.44 LMIQFEE68 pKa = 4.82 GDD70 pKa = 3.4 NEE72 pKa = 4.51 SPSAYY77 pKa = 10.35 YY78 pKa = 10.6 SFSLQDD84 pKa = 3.85 FYY86 pKa = 11.78 EE87 pKa = 4.9 FMALFLSGQEE97 pKa = 4.19 PEE99 pKa = 4.64 DD100 pKa = 3.81 EE101 pKa = 4.27 EE102 pKa = 5.04 EE103 pKa = 4.53 EE104 pKa = 4.5 GDD106 pKa = 3.81 EE107 pKa = 4.17 QEE109 pKa = 4.56 YY110 pKa = 11.02 GPMHH114 pKa = 6.52 HH115 pKa = 7.07 LGHH118 pKa = 7.27 LLHH121 pKa = 7.07 HH122 pKa = 6.51 IVEE125 pKa = 4.94 EE126 pKa = 4.63 GNSLKK131 pKa = 10.7 PP132 pKa = 3.52
Molecular weight: 15.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.948
IPC_protein 3.91
Toseland 3.719
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.783
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.177
Thurlkill 3.757
EMBOSS 3.808
Sillero 4.037
Patrickios 1.901
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A2N5M552|A0A2N5M552_9BACI Integrase catalytic domain-containing protein OS=Peribacillus deserti OX=673318 GN=CUU66_12815 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.24 QPNKK9 pKa = 8.16 RR10 pKa = 11.84 KK11 pKa = 9.54 HH12 pKa = 5.99 SKK14 pKa = 8.91 VHH16 pKa = 5.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSSKK25 pKa = 10.48 NGRR28 pKa = 11.84 KK29 pKa = 9.07 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.81 GRR39 pKa = 11.84 KK40 pKa = 8.75 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.391
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.384
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.106
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.993
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4462
0
4462
1228183
17
1517
275.3
30.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.237 ± 0.04
0.746 ± 0.011
5.005 ± 0.029
7.321 ± 0.042
4.605 ± 0.032
7.07 ± 0.039
2.108 ± 0.018
7.781 ± 0.032
7.026 ± 0.04
9.855 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.694 ± 0.019
4.301 ± 0.028
3.719 ± 0.022
3.567 ± 0.02
4.179 ± 0.026
6.383 ± 0.031
5.205 ± 0.023
6.677 ± 0.031
1.025 ± 0.015
3.493 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here