Neorhizobium sp. SOG26
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4290 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346YJY4|A0A346YJY4_9RHIZ Heme lyase NrfEFG subunit NrfE OS=Neorhizobium sp. SOG26 OX=2060726 GN=CYG48_03130 PE=3 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.3 AILILASAAAMTASSAVYY20 pKa = 9.91 AADD23 pKa = 3.27 AVEE26 pKa = 5.0 TIPAAPEE33 pKa = 4.32 AIEE36 pKa = 4.25 TVSAFTWSGPYY47 pKa = 9.52 IGLHH51 pKa = 6.27 GGYY54 pKa = 10.3 AWGNADD60 pKa = 3.58 STLLPGGSDD69 pKa = 3.51 DD70 pKa = 4.31 FDD72 pKa = 4.2 GGRR75 pKa = 11.84 FGAFVGYY82 pKa = 9.84 NWQFSNGFVAGIEE95 pKa = 4.11 GDD97 pKa = 4.35 VNYY100 pKa = 10.74 DD101 pKa = 3.13 WNEE104 pKa = 3.51 NTYY107 pKa = 11.11 AGVDD111 pKa = 3.55 AEE113 pKa = 4.79 SGFSGSVRR121 pKa = 11.84 GRR123 pKa = 11.84 VGYY126 pKa = 10.86 AFDD129 pKa = 4.09 RR130 pKa = 11.84 ALLFAAGGWTATNVNVDD147 pKa = 3.36 GGGVDD152 pKa = 5.83 DD153 pKa = 6.94 DD154 pKa = 4.61 DD155 pKa = 4.73 TLHH158 pKa = 6.42 GWTIGGGLDD167 pKa = 3.56 YY168 pKa = 11.38 AFTDD172 pKa = 3.51 NVFGRR177 pKa = 11.84 VEE179 pKa = 3.57 YY180 pKa = 10.15 RR181 pKa = 11.84 YY182 pKa = 10.56 NDD184 pKa = 3.67 FGSGDD189 pKa = 3.36 IAGTDD194 pKa = 3.06 VDD196 pKa = 4.83 FDD198 pKa = 3.61 QHH200 pKa = 5.44 VVNVGIAVKK209 pKa = 10.16 FF210 pKa = 3.63
Molecular weight: 21.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.567
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.783
Rodwell 3.63
Grimsley 3.478
Solomon 3.808
Lehninger 3.757
Nozaki 3.935
DTASelect 4.215
Thurlkill 3.643
EMBOSS 3.783
Sillero 3.935
Patrickios 1.074
IPC_peptide 3.795
IPC2_peptide 3.897
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|A0A346YJ05|A0A346YJ05_9RHIZ Two-component system response regulator OS=Neorhizobium sp. SOG26 OX=2060726 GN=CYG48_01190 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATNGGRR28 pKa = 11.84 KK29 pKa = 8.93 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4290
0
4290
1337437
34
2831
311.8
33.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.685 ± 0.049
0.781 ± 0.011
5.563 ± 0.026
6.036 ± 0.034
3.897 ± 0.028
8.213 ± 0.03
1.995 ± 0.017
5.613 ± 0.027
3.851 ± 0.031
9.94 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.68 ± 0.016
2.873 ± 0.017
4.954 ± 0.025
3.228 ± 0.02
6.604 ± 0.033
5.741 ± 0.025
5.308 ± 0.02
7.444 ± 0.03
1.273 ± 0.015
2.323 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here