Amino acid dipepetide frequency for Sphingomonas sp. BK235

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
26.155AlaAla: 26.155 ± 0.236
1.032AlaCys: 1.032 ± 0.027
7.95AlaAsp: 7.95 ± 0.078
7.788AlaGlu: 7.788 ± 0.081
4.225AlaPhe: 4.225 ± 0.063
13.333AlaGly: 13.333 ± 0.125
2.512AlaHis: 2.512 ± 0.045
6.627AlaIle: 6.627 ± 0.082
2.997AlaLys: 2.997 ± 0.062
16.61AlaLeu: 16.61 ± 0.177
3.636AlaMet: 3.636 ± 0.051
2.865AlaAsn: 2.865 ± 0.047
8.382AlaPro: 8.382 ± 0.1
4.653AlaGln: 4.653 ± 0.066
12.946AlaArg: 12.946 ± 0.139
6.657AlaSer: 6.657 ± 0.07
8.471AlaThr: 8.471 ± 0.088
10.297AlaVal: 10.297 ± 0.105
1.869AlaTrp: 1.869 ± 0.043
2.603AlaTyr: 2.603 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
0.987CysAla: 0.987 ± 0.025
0.082CysCys: 0.082 ± 0.008
0.461CysAsp: 0.461 ± 0.019
0.285CysGlu: 0.285 ± 0.013
0.226CysPhe: 0.226 ± 0.014
0.749CysGly: 0.749 ± 0.025
0.162CysHis: 0.162 ± 0.011
0.225CysIle: 0.225 ± 0.012
0.09CysLys: 0.09 ± 0.008
0.628CysLeu: 0.628 ± 0.021
0.105CysMet: 0.105 ± 0.009
0.133CysAsn: 0.133 ± 0.009
0.367CysPro: 0.367 ± 0.017
0.142CysGln: 0.142 ± 0.011
0.531CysArg: 0.531 ± 0.021
0.352CysSer: 0.352 ± 0.017
0.351CysThr: 0.351 ± 0.017
0.543CysVal: 0.543 ± 0.02
0.099CysTrp: 0.099 ± 0.008
0.161CysTyr: 0.161 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
8.766AspAla: 8.766 ± 0.086
0.371AspCys: 0.371 ± 0.017
3.507AspAsp: 3.507 ± 0.06
3.23AspGlu: 3.23 ± 0.054
1.931AspPhe: 1.931 ± 0.041
5.798AspGly: 5.798 ± 0.071
1.309AspHis: 1.309 ± 0.031
2.095AspIle: 2.095 ± 0.045
1.159AspLys: 1.159 ± 0.034
5.663AspLeu: 5.663 ± 0.065
1.129AspMet: 1.129 ± 0.03
1.161AspAsn: 1.161 ± 0.029
4.069AspPro: 4.069 ± 0.058
1.843AspGln: 1.843 ± 0.032
5.42AspArg: 5.42 ± 0.062
2.332AspSer: 2.332 ± 0.038
2.877AspThr: 2.877 ± 0.05
4.567AspVal: 4.567 ± 0.055
1.172AspTrp: 1.172 ± 0.029
1.691AspTyr: 1.691 ± 0.038
0.0AspXaa: 0.0 ± 0.0
Glu
7.378GluAla: 7.378 ± 0.09
0.204GluCys: 0.204 ± 0.011
2.247GluAsp: 2.247 ± 0.041
2.493GluGlu: 2.493 ± 0.052
1.186GluPhe: 1.186 ± 0.029
3.96GluGly: 3.96 ± 0.057
1.008GluHis: 1.008 ± 0.028
2.218GluIle: 2.218 ± 0.043
1.3GluLys: 1.3 ± 0.04
4.964GluLeu: 4.964 ± 0.071
1.078GluMet: 1.078 ± 0.03
0.948GluAsn: 0.948 ± 0.028
2.521GluPro: 2.521 ± 0.042
2.077GluGln: 2.077 ± 0.041
5.835GluArg: 5.835 ± 0.072
1.984GluSer: 1.984 ± 0.041
2.754GluThr: 2.754 ± 0.042
3.523GluVal: 3.523 ± 0.051
0.701GluTrp: 0.701 ± 0.02
0.817GluTyr: 0.817 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.818PheAla: 4.818 ± 0.056
0.244PheCys: 0.244 ± 0.015
2.539PheAsp: 2.539 ± 0.044
1.642PheGlu: 1.642 ± 0.033
1.113PhePhe: 1.113 ± 0.031
3.431PheGly: 3.431 ± 0.052
0.674PheHis: 0.674 ± 0.023
0.999PheIle: 0.999 ± 0.028
0.62PheLys: 0.62 ± 0.023
2.854PheLeu: 2.854 ± 0.052
0.575PheMet: 0.575 ± 0.02
0.894PheAsn: 0.894 ± 0.028
1.403PhePro: 1.403 ± 0.03
0.869PheGln: 0.869 ± 0.022
2.254PheArg: 2.254 ± 0.043
1.642PheSer: 1.642 ± 0.037
1.934PheThr: 1.934 ± 0.037
2.633PheVal: 2.633 ± 0.049
0.441PheTrp: 0.441 ± 0.017
0.905PheTyr: 0.905 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
12.566GlyAla: 12.566 ± 0.112
0.769GlyCys: 0.769 ± 0.023
5.204GlyAsp: 5.204 ± 0.068
4.837GlyGlu: 4.837 ± 0.07
3.459GlyPhe: 3.459 ± 0.055
8.548GlyGly: 8.548 ± 0.107
1.737GlyHis: 1.737 ± 0.035
3.702GlyIle: 3.702 ± 0.055
2.372GlyLys: 2.372 ± 0.041
8.362GlyLeu: 8.362 ± 0.087
2.007GlyMet: 2.007 ± 0.039
2.046GlyAsn: 2.046 ± 0.051
3.315GlyPro: 3.315 ± 0.053
2.78GlyGln: 2.78 ± 0.047
7.163GlyArg: 7.163 ± 0.077
4.608GlySer: 4.608 ± 0.071
5.192GlyThr: 5.192 ± 0.071
6.914GlyVal: 6.914 ± 0.068
1.782GlyTrp: 1.782 ± 0.043
2.43GlyTyr: 2.43 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.692HisAla: 2.692 ± 0.048
0.165HisCys: 0.165 ± 0.011
1.318HisAsp: 1.318 ± 0.033
0.925HisGlu: 0.925 ± 0.028
0.682HisPhe: 0.682 ± 0.025
1.866HisGly: 1.866 ± 0.04
0.581HisHis: 0.581 ± 0.025
0.602HisIle: 0.602 ± 0.022
0.265HisLys: 0.265 ± 0.015
1.868HisLeu: 1.868 ± 0.036
0.338HisMet: 0.338 ± 0.016
0.375HisAsn: 0.375 ± 0.016
1.268HisPro: 1.268 ± 0.036
0.574HisGln: 0.574 ± 0.02
1.658HisArg: 1.658 ± 0.032
0.737HisSer: 0.737 ± 0.024
0.674HisThr: 0.674 ± 0.02
1.532HisVal: 1.532 ± 0.036
0.363HisTrp: 0.363 ± 0.018
0.577HisTyr: 0.577 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.281IleAla: 7.281 ± 0.082
0.28IleCys: 0.28 ± 0.014
3.534IleAsp: 3.534 ± 0.049
2.859IleGlu: 2.859 ± 0.048
1.075IlePhe: 1.075 ± 0.028
4.499IleGly: 4.499 ± 0.065
0.705IleHis: 0.705 ± 0.022
1.267IleIle: 1.267 ± 0.037
0.846IleLys: 0.846 ± 0.026
2.935IleLeu: 2.935 ± 0.05
0.565IleMet: 0.565 ± 0.018
1.089IleAsn: 1.089 ± 0.027
1.742IlePro: 1.742 ± 0.033
0.919IleGln: 0.919 ± 0.028
2.761IleArg: 2.761 ± 0.046
1.788IleSer: 1.788 ± 0.042
2.195IleThr: 2.195 ± 0.044
3.827IleVal: 3.827 ± 0.047
0.435IleTrp: 0.435 ± 0.02
0.824IleTyr: 0.824 ± 0.021
0.0IleXaa: 0.0 ± 0.0
Lys
2.813LysAla: 2.813 ± 0.057
0.085LysCys: 0.085 ± 0.009
1.043LysAsp: 1.043 ± 0.026
0.844LysGlu: 0.844 ± 0.027
0.566LysPhe: 0.566 ± 0.024
1.861LysGly: 1.861 ± 0.043
0.37LysHis: 0.37 ± 0.017
0.929LysIle: 0.929 ± 0.031
0.701LysLys: 0.701 ± 0.023
2.327LysLeu: 2.327 ± 0.049
0.517LysMet: 0.517 ± 0.022
0.486LysAsn: 0.486 ± 0.024
1.379LysPro: 1.379 ± 0.034
0.651LysGln: 0.651 ± 0.02
1.841LysArg: 1.841 ± 0.039
1.111LysSer: 1.111 ± 0.03
1.22LysThr: 1.22 ± 0.029
1.791LysVal: 1.791 ± 0.041
0.264LysTrp: 0.264 ± 0.013
0.414LysTyr: 0.414 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
16.23LeuAla: 16.23 ± 0.147
0.713LeuCys: 0.713 ± 0.02
6.527LeuAsp: 6.527 ± 0.078
4.192LeuGlu: 4.192 ± 0.053
3.477LeuPhe: 3.477 ± 0.061
8.745LeuGly: 8.745 ± 0.09
1.855LeuHis: 1.855 ± 0.037
4.091LeuIle: 4.091 ± 0.053
2.091LeuLys: 2.091 ± 0.046
10.415LeuLeu: 10.415 ± 0.128
1.773LeuMet: 1.773 ± 0.041
2.129LeuAsn: 2.129 ± 0.041
6.015LeuPro: 6.015 ± 0.068
2.291LeuGln: 2.291 ± 0.04
7.982LeuArg: 7.982 ± 0.088
5.466LeuSer: 5.466 ± 0.072
5.816LeuThr: 5.816 ± 0.061
8.024LeuVal: 8.024 ± 0.083
1.297LeuTrp: 1.297 ± 0.031
2.02LeuTyr: 2.02 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.81MetAla: 2.81 ± 0.049
0.114MetCys: 0.114 ± 0.009
0.88MetAsp: 0.88 ± 0.027
0.783MetGlu: 0.783 ± 0.022
0.526MetPhe: 0.526 ± 0.02
1.456MetGly: 1.456 ± 0.035
0.332MetHis: 0.332 ± 0.017
1.071MetIle: 1.071 ± 0.025
0.639MetLys: 0.639 ± 0.024
2.109MetLeu: 2.109 ± 0.039
0.517MetMet: 0.517 ± 0.02
0.52MetAsn: 0.52 ± 0.019
1.279MetPro: 1.279 ± 0.029
0.535MetGln: 0.535 ± 0.018
1.705MetArg: 1.705 ± 0.04
1.237MetSer: 1.237 ± 0.03
1.656MetThr: 1.656 ± 0.035
1.479MetVal: 1.479 ± 0.032
0.19MetTrp: 0.19 ± 0.011
0.229MetTyr: 0.229 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.986AsnAla: 2.986 ± 0.049
0.171AsnCys: 0.171 ± 0.011
1.312AsnAsp: 1.312 ± 0.032
0.917AsnGlu: 0.917 ± 0.026
0.747AsnPhe: 0.747 ± 0.025
2.132AsnGly: 2.132 ± 0.05
0.403AsnHis: 0.403 ± 0.018
0.923AsnIle: 0.923 ± 0.025
0.482AsnLys: 0.482 ± 0.02
2.205AsnLeu: 2.205 ± 0.05
0.43AsnMet: 0.43 ± 0.017
0.663AsnAsn: 0.663 ± 0.027
1.472AsnPro: 1.472 ± 0.035
0.653AsnGln: 0.653 ± 0.023
1.619AsnArg: 1.619 ± 0.038
1.024AsnSer: 1.024 ± 0.047
1.073AsnThr: 1.073 ± 0.033
1.812AsnVal: 1.812 ± 0.041
0.37AsnTrp: 0.37 ± 0.017
0.659AsnTyr: 0.659 ± 0.028
0.0AsnXaa: 0.0 ± 0.0
Pro
8.897ProAla: 8.897 ± 0.099
0.29ProCys: 0.29 ± 0.014
3.673ProAsp: 3.673 ± 0.051
3.056ProGlu: 3.056 ± 0.048
1.772ProPhe: 1.772 ± 0.034
5.175ProGly: 5.175 ± 0.063
1.052ProHis: 1.052 ± 0.031
2.194ProIle: 2.194 ± 0.044
1.046ProLys: 1.046 ± 0.031
5.425ProLeu: 5.425 ± 0.067
0.962ProMet: 0.962 ± 0.025
1.084ProAsn: 1.084 ± 0.029
3.201ProPro: 3.201 ± 0.067
1.502ProGln: 1.502 ± 0.033
4.103ProArg: 4.103 ± 0.058
2.806ProSer: 2.806 ± 0.044
2.766ProThr: 2.766 ± 0.049
4.28ProVal: 4.28 ± 0.057
0.697ProTrp: 0.697 ± 0.02
1.141ProTyr: 1.141 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
4.028GlnAla: 4.028 ± 0.061
0.171GlnCys: 0.171 ± 0.011
1.299GlnAsp: 1.299 ± 0.028
1.116GlnGlu: 1.116 ± 0.028
0.929GlnPhe: 0.929 ± 0.029
2.296GlnGly: 2.296 ± 0.045
0.611GlnHis: 0.611 ± 0.021
1.383GlnIle: 1.383 ± 0.036
0.655GlnLys: 0.655 ± 0.024
3.295GlnLeu: 3.295 ± 0.057
0.626GlnMet: 0.626 ± 0.023
0.638GlnAsn: 0.638 ± 0.026
1.779GlnPro: 1.779 ± 0.036
1.245GlnGln: 1.245 ± 0.034
2.983GlnArg: 2.983 ± 0.046
1.461GlnSer: 1.461 ± 0.034
1.541GlnThr: 1.541 ± 0.034
2.43GlnVal: 2.43 ± 0.041
0.391GlnTrp: 0.391 ± 0.016
0.612GlnTyr: 0.612 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
12.284ArgAla: 12.284 ± 0.122
0.54ArgCys: 0.54 ± 0.023
5.512ArgAsp: 5.512 ± 0.065
3.933ArgGlu: 3.933 ± 0.058
3.326ArgPhe: 3.326 ± 0.045
6.366ArgGly: 6.366 ± 0.072
1.868ArgHis: 1.868 ± 0.037
3.421ArgIle: 3.421 ± 0.054
1.409ArgLys: 1.409 ± 0.038
9.411ArgLeu: 9.411 ± 0.113
1.795ArgMet: 1.795 ± 0.03
1.568ArgAsn: 1.568 ± 0.037
4.229ArgPro: 4.229 ± 0.058
2.614ArgGln: 2.614 ± 0.046
7.696ArgArg: 7.696 ± 0.091
3.777ArgSer: 3.777 ± 0.056
3.921ArgThr: 3.921 ± 0.06
6.323ArgVal: 6.323 ± 0.071
1.601ArgTrp: 1.601 ± 0.037
2.208ArgTyr: 2.208 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
6.479SerAla: 6.479 ± 0.071
0.334SerCys: 0.334 ± 0.019
2.915SerAsp: 2.915 ± 0.045
2.124SerGlu: 2.124 ± 0.041
1.942SerPhe: 1.942 ± 0.043
5.066SerGly: 5.066 ± 0.071
0.849SerHis: 0.849 ± 0.026
2.205SerIle: 2.205 ± 0.042
1.053SerLys: 1.053 ± 0.03
4.704SerLeu: 4.704 ± 0.061
0.858SerMet: 0.858 ± 0.028
1.275SerAsn: 1.275 ± 0.038
2.793SerPro: 2.793 ± 0.045
1.294SerGln: 1.294 ± 0.03
3.391SerArg: 3.391 ± 0.041
2.538SerSer: 2.538 ± 0.052
2.544SerThr: 2.544 ± 0.054
3.629SerVal: 3.629 ± 0.06
0.756SerTrp: 0.756 ± 0.023
1.437SerTyr: 1.437 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
7.383ThrAla: 7.383 ± 0.076
0.35ThrCys: 0.35 ± 0.019
2.963ThrAsp: 2.963 ± 0.047
2.142ThrGlu: 2.142 ± 0.041
1.823ThrPhe: 1.823 ± 0.038
5.392ThrGly: 5.392 ± 0.064
0.909ThrHis: 0.909 ± 0.024
2.884ThrIle: 2.884 ± 0.051
1.084ThrLys: 1.084 ± 0.028
6.521ThrLeu: 6.521 ± 0.066
1.054ThrMet: 1.054 ± 0.03
1.252ThrAsn: 1.252 ± 0.034
3.78ThrPro: 3.78 ± 0.063
1.45ThrGln: 1.45 ± 0.035
4.105ThrArg: 4.105 ± 0.058
2.678ThrSer: 2.678 ± 0.052
2.802ThrThr: 2.802 ± 0.05
4.513ThrVal: 4.513 ± 0.059
0.714ThrTrp: 0.714 ± 0.023
1.211ThrTyr: 1.211 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
12.562ValAla: 12.562 ± 0.128
0.458ValCys: 0.458 ± 0.017
4.74ValAsp: 4.74 ± 0.049
4.423ValGlu: 4.423 ± 0.066
2.194ValPhe: 2.194 ± 0.042
5.938ValGly: 5.938 ± 0.071
1.282ValHis: 1.282 ± 0.032
3.353ValIle: 3.353 ± 0.05
1.576ValLys: 1.576 ± 0.037
6.976ValLeu: 6.976 ± 0.084
1.43ValMet: 1.43 ± 0.038
1.909ValAsn: 1.909 ± 0.044
4.3ValPro: 4.3 ± 0.06
1.988ValGln: 1.988 ± 0.048
5.962ValArg: 5.962 ± 0.077
4.009ValSer: 4.009 ± 0.063
5.281ValThr: 5.281 ± 0.061
5.864ValVal: 5.864 ± 0.071
0.869ValTrp: 0.869 ± 0.025
1.459ValTyr: 1.459 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.421TrpAla: 1.421 ± 0.036
0.128TrpCys: 0.128 ± 0.009
0.769TrpAsp: 0.769 ± 0.027
0.553TrpGlu: 0.553 ± 0.023
0.54TrpPhe: 0.54 ± 0.018
1.025TrpGly: 1.025 ± 0.029
0.364TrpHis: 0.364 ± 0.016
0.602TrpIle: 0.602 ± 0.022
0.372TrpLys: 0.372 ± 0.017
1.778TrpLeu: 1.778 ± 0.038
0.319TrpMet: 0.319 ± 0.015
0.395TrpAsn: 0.395 ± 0.015
0.731TrpPro: 0.731 ± 0.025
0.667TrpGln: 0.667 ± 0.023
1.683TrpArg: 1.683 ± 0.033
0.947TrpSer: 0.947 ± 0.027
0.871TrpThr: 0.871 ± 0.026
0.869TrpVal: 0.869 ± 0.023
0.314TrpTrp: 0.314 ± 0.017
0.326TrpTyr: 0.326 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.072TyrAla: 3.072 ± 0.046
0.179TyrCys: 0.179 ± 0.011
1.696TyrAsp: 1.696 ± 0.038
1.055TyrGlu: 1.055 ± 0.026
0.742TyrPhe: 0.742 ± 0.024
2.158TyrGly: 2.158 ± 0.042
0.477TyrHis: 0.477 ± 0.018
0.633TyrIle: 0.633 ± 0.022
0.458TyrLys: 0.458 ± 0.019
2.102TyrLeu: 2.102 ± 0.04
0.351TyrMet: 0.351 ± 0.019
0.622TyrAsn: 0.622 ± 0.024
1.098TyrPro: 1.098 ± 0.028
0.741TyrGln: 0.741 ± 0.025
2.16TyrArg: 2.16 ± 0.036
1.035TyrSer: 1.035 ± 0.031
1.069TyrThr: 1.069 ± 0.031
1.726TyrVal: 1.726 ± 0.039
0.348TyrTrp: 0.348 ± 0.015
0.611TyrTyr: 0.611 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4418 proteins (1441282 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski